LT3404112T - Fermento analizė su dublikuotais fluoroforais - Google Patents

Fermento analizė su dublikuotais fluoroforais

Info

Publication number
LT3404112T
LT3404112T LTEP18176287.3T LT18176287T LT3404112T LT 3404112 T LT3404112 T LT 3404112T LT 18176287 T LT18176287 T LT 18176287T LT 3404112 T LT3404112 T LT 3404112T
Authority
LT
Lithuania
Prior art keywords
fluorophores
duplicate
enzyme assay
assay
enzyme
Prior art date
Application number
LTEP18176287.3T
Other languages
English (en)
Inventor
Ward C. Tucker
Original Assignee
Biomadison, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomadison, Inc. filed Critical Biomadison, Inc.
Publication of LT3404112T publication Critical patent/LT3404112T/lt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24069Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/533Production of labelled immunochemicals with fluorescent label
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/95Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/948Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/952Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
LTEP18176287.3T 2013-10-25 2014-10-24 Fermento analizė su dublikuotais fluoroforais LT3404112T (lt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361895533P 2013-10-25 2013-10-25
US201361897352P 2013-10-30 2013-10-30
US201462014586P 2014-06-19 2014-06-19
US201462058532P 2014-10-01 2014-10-01

Publications (1)

Publication Number Publication Date
LT3404112T true LT3404112T (lt) 2020-12-28

Family

ID=52001049

Family Applications (2)

Application Number Title Priority Date Filing Date
LTEP14805702.9T LT3060677T (lt) 2013-10-25 2014-10-24 Fermentų tyrimo su dublikuotais fluoroforais būdas
LTEP18176287.3T LT3404112T (lt) 2013-10-25 2014-10-24 Fermento analizė su dublikuotais fluoroforais

Family Applications Before (1)

Application Number Title Priority Date Filing Date
LTEP14805702.9T LT3060677T (lt) 2013-10-25 2014-10-24 Fermentų tyrimo su dublikuotais fluoroforais būdas

Country Status (13)

Country Link
US (3) US20150118701A1 (lt)
EP (2) EP3404112B1 (lt)
JP (2) JP6636919B2 (lt)
KR (1) KR20160103977A (lt)
CN (2) CN113073129A (lt)
AU (1) AU2014339884B2 (lt)
CA (1) CA2928609C (lt)
ES (2) ES2686141T3 (lt)
LT (2) LT3060677T (lt)
MX (2) MX2016005270A (lt)
RU (1) RU2683207C2 (lt)
TW (1) TWI647237B (lt)
WO (1) WO2015061738A1 (lt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015021433A1 (en) * 2013-08-09 2015-02-12 Biomadison, Inc. Botulinum toxin assay with improved sensitivity
AU2015301737B2 (en) 2014-08-12 2021-01-28 Biomadison, Inc. Botulinum neurotoxins with modified light chain specifity and methods for producing same
US12019066B2 (en) * 2016-05-16 2024-06-25 Biomadison, Inc. Assay with synaptobrevin based moiety
US11396499B2 (en) 2018-12-12 2022-07-26 University Of Washington Lysosomal acid lipase assay
WO2022216778A1 (en) * 2021-04-06 2022-10-13 Berkeley Lights, Inc. Methods of microfluidic assay and bioproduction from non-mammalian cells and kits therefor

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3863372B2 (ja) * 1998-10-30 2006-12-27 セロミックス インコーポレイテッド プロテアーゼバイオセンサーをコードする組換え核酸、組換え発現ベクター、プロテアーゼバイオセンサー、化合物を同定する方法、及び化合物を同定するためのキット
WO2002048174A2 (en) 2000-12-15 2002-06-20 Stratagene Dimeric fluorescent polypeptides
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
US8137924B2 (en) * 2003-12-19 2012-03-20 Wisconsin Alumni Research Foundation Method and compositions for detecting botulinum neurotoxin
ES2351942T3 (es) * 2005-04-05 2011-02-14 Allergan Inc Analisis de la actividad de una toxina clostridial.
EP1997885A1 (en) 2007-05-29 2008-12-03 AXXAM S.p.A. Fluorescent proteins and methods of use thereof
US8753831B2 (en) * 2007-06-05 2014-06-17 City Of Hope Methods for detection of botulinum neurotoxin
SG174353A1 (en) * 2009-03-13 2011-11-28 Allergan Inc Immuno-based retargeted endopeptidase activity assays
US9453254B2 (en) * 2009-10-16 2016-09-27 Biomadison, Inc. Resonance energy transfer assay with synaptobrevin substrate moiety
EP2715355B1 (en) * 2011-06-01 2017-03-08 Biomadison, Inc. Non-fret botulinum assay
WO2015021433A1 (en) 2013-08-09 2015-02-12 Biomadison, Inc. Botulinum toxin assay with improved sensitivity

Also Published As

Publication number Publication date
MX2016005270A (es) 2017-02-02
JP2016533733A (ja) 2016-11-04
LT3060677T (lt) 2018-08-27
US20240117408A1 (en) 2024-04-11
CN106414763B (zh) 2021-04-09
RU2016118286A (ru) 2017-11-30
AU2014339884A1 (en) 2016-05-12
ES2686141T3 (es) 2018-10-16
TW201531481A (zh) 2015-08-16
WO2015061738A1 (en) 2015-04-30
CN113073129A (zh) 2021-07-06
RU2016118286A3 (lt) 2018-05-21
EP3404112A1 (en) 2018-11-21
EP3060677B1 (en) 2018-07-04
JP6636919B2 (ja) 2020-01-29
JP2020062032A (ja) 2020-04-23
EP3060677A1 (en) 2016-08-31
KR20160103977A (ko) 2016-09-02
AU2014339884B2 (en) 2020-03-05
CA2928609C (en) 2022-03-15
CN106414763A (zh) 2017-02-15
MX2019013952A (es) 2020-01-30
EP3404112B1 (en) 2020-08-26
TWI647237B (zh) 2019-01-11
CA2928609A1 (en) 2015-04-30
US20150118701A1 (en) 2015-04-30
US11685945B2 (en) 2023-06-27
ES2829612T3 (es) 2021-06-01
RU2683207C2 (ru) 2019-03-26
WO2015061738A4 (en) 2015-07-02
US20210032675A1 (en) 2021-02-04

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