MX2019013952A - Ensayo enzimatico con fluoroforos duplicados. - Google Patents

Ensayo enzimatico con fluoroforos duplicados.

Info

Publication number
MX2019013952A
MX2019013952A MX2019013952A MX2019013952A MX2019013952A MX 2019013952 A MX2019013952 A MX 2019013952A MX 2019013952 A MX2019013952 A MX 2019013952A MX 2019013952 A MX2019013952 A MX 2019013952A MX 2019013952 A MX2019013952 A MX 2019013952A
Authority
MX
Mexico
Prior art keywords
region
fluorophores
duplicate
enzyme assay
cleavage site
Prior art date
Application number
MX2019013952A
Other languages
English (en)
Inventor
C Tucker Ward
Original Assignee
Biomadison Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomadison Inc filed Critical Biomadison Inc
Publication of MX2019013952A publication Critical patent/MX2019013952A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/952—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se describen composiciones y métodos que proporcionan un ensayo a base de células rápido, sensible y exacto para la actividad enzimática, en particular para las actividades enzimáticas asociadas con las toxinas botulínicas. Se proporciona una célula que expresa una construcción que incluye una región de anclaje, un sitio de escisión y una región de reporte que tiene dos o más péptidos reporteros idénticos. La actividad enzimática en el sitio de escisión libera la región reportera en el citosol, donde se producen varios eventos de degradación. El cambio observado en la señal es proporcional a la actividad enzimática.
MX2019013952A 2013-10-25 2016-04-22 Ensayo enzimatico con fluoroforos duplicados. MX2019013952A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361895533P 2013-10-25 2013-10-25
US201361897352P 2013-10-30 2013-10-30
US201462014586P 2014-06-19 2014-06-19
US201462058532P 2014-10-01 2014-10-01

Publications (1)

Publication Number Publication Date
MX2019013952A true MX2019013952A (es) 2020-01-30

Family

ID=52001049

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2016005270A MX2016005270A (es) 2013-10-25 2014-10-24 Ensayo enzimatico con fluoroforos duplicados.
MX2019013952A MX2019013952A (es) 2013-10-25 2016-04-22 Ensayo enzimatico con fluoroforos duplicados.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2016005270A MX2016005270A (es) 2013-10-25 2014-10-24 Ensayo enzimatico con fluoroforos duplicados.

Country Status (13)

Country Link
US (3) US20150118701A1 (es)
EP (2) EP3404112B1 (es)
JP (2) JP6636919B2 (es)
KR (1) KR20160103977A (es)
CN (2) CN113073129A (es)
AU (1) AU2014339884B2 (es)
CA (1) CA2928609C (es)
ES (2) ES2686141T3 (es)
LT (2) LT3060677T (es)
MX (2) MX2016005270A (es)
RU (1) RU2683207C2 (es)
TW (1) TWI647237B (es)
WO (1) WO2015061738A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015021433A1 (en) * 2013-08-09 2015-02-12 Biomadison, Inc. Botulinum toxin assay with improved sensitivity
AU2015301737B2 (en) 2014-08-12 2021-01-28 Biomadison, Inc. Botulinum neurotoxins with modified light chain specifity and methods for producing same
US12019066B2 (en) * 2016-05-16 2024-06-25 Biomadison, Inc. Assay with synaptobrevin based moiety
US11396499B2 (en) 2018-12-12 2022-07-26 University Of Washington Lysosomal acid lipase assay
WO2022216778A1 (en) * 2021-04-06 2022-10-13 Berkeley Lights, Inc. Methods of microfluidic assay and bioproduction from non-mammalian cells and kits therefor

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3863372B2 (ja) * 1998-10-30 2006-12-27 セロミックス インコーポレイテッド プロテアーゼバイオセンサーをコードする組換え核酸、組換え発現ベクター、プロテアーゼバイオセンサー、化合物を同定する方法、及び化合物を同定するためのキット
WO2002048174A2 (en) 2000-12-15 2002-06-20 Stratagene Dimeric fluorescent polypeptides
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
US8137924B2 (en) * 2003-12-19 2012-03-20 Wisconsin Alumni Research Foundation Method and compositions for detecting botulinum neurotoxin
ES2351942T3 (es) * 2005-04-05 2011-02-14 Allergan Inc Analisis de la actividad de una toxina clostridial.
EP1997885A1 (en) 2007-05-29 2008-12-03 AXXAM S.p.A. Fluorescent proteins and methods of use thereof
US8753831B2 (en) * 2007-06-05 2014-06-17 City Of Hope Methods for detection of botulinum neurotoxin
SG174353A1 (en) * 2009-03-13 2011-11-28 Allergan Inc Immuno-based retargeted endopeptidase activity assays
US9453254B2 (en) * 2009-10-16 2016-09-27 Biomadison, Inc. Resonance energy transfer assay with synaptobrevin substrate moiety
EP2715355B1 (en) * 2011-06-01 2017-03-08 Biomadison, Inc. Non-fret botulinum assay
WO2015021433A1 (en) 2013-08-09 2015-02-12 Biomadison, Inc. Botulinum toxin assay with improved sensitivity

Also Published As

Publication number Publication date
MX2016005270A (es) 2017-02-02
JP2016533733A (ja) 2016-11-04
LT3060677T (lt) 2018-08-27
US20240117408A1 (en) 2024-04-11
CN106414763B (zh) 2021-04-09
RU2016118286A (ru) 2017-11-30
AU2014339884A1 (en) 2016-05-12
ES2686141T3 (es) 2018-10-16
TW201531481A (zh) 2015-08-16
WO2015061738A1 (en) 2015-04-30
CN113073129A (zh) 2021-07-06
RU2016118286A3 (es) 2018-05-21
EP3404112A1 (en) 2018-11-21
EP3060677B1 (en) 2018-07-04
JP6636919B2 (ja) 2020-01-29
JP2020062032A (ja) 2020-04-23
EP3060677A1 (en) 2016-08-31
KR20160103977A (ko) 2016-09-02
AU2014339884B2 (en) 2020-03-05
LT3404112T (lt) 2020-12-28
CA2928609C (en) 2022-03-15
CN106414763A (zh) 2017-02-15
EP3404112B1 (en) 2020-08-26
TWI647237B (zh) 2019-01-11
CA2928609A1 (en) 2015-04-30
US20150118701A1 (en) 2015-04-30
US11685945B2 (en) 2023-06-27
ES2829612T3 (es) 2021-06-01
RU2683207C2 (ru) 2019-03-26
WO2015061738A4 (en) 2015-07-02
US20210032675A1 (en) 2021-02-04

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