JP2005520000A5 - - Google Patents
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- JP2005520000A5 JP2005520000A5 JP2004541415A JP2004541415A JP2005520000A5 JP 2005520000 A5 JP2005520000 A5 JP 2005520000A5 JP 2004541415 A JP2004541415 A JP 2004541415A JP 2004541415 A JP2004541415 A JP 2004541415A JP 2005520000 A5 JP2005520000 A5 JP 2005520000A5
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- Prior art keywords
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000000758 substrate Substances 0.000 claims description 81
- 108030001720 Bontoxilysin Proteins 0.000 claims description 50
- 238000003776 cleavage reaction Methods 0.000 claims description 29
- 230000007017 scission Effects 0.000 claims description 29
- 238000000862 absorption spectrum Methods 0.000 claims description 4
- 238000000295 emission spectrum Methods 0.000 claims description 4
- 239000000370 acceptor Substances 0.000 claims 38
- 108090000765 processed proteins & peptides Proteins 0.000 claims 26
- 238000000034 method Methods 0.000 claims 19
- 230000000694 effects Effects 0.000 claims 16
- 239000003053 toxin Substances 0.000 claims 13
- 231100000765 toxin Toxicity 0.000 claims 13
- 108091005804 Peptidases Proteins 0.000 claims 11
- 239000004365 Protease Substances 0.000 claims 11
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 11
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 9
- 125000000539 amino acid group Chemical group 0.000 claims 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims 7
- 238000002165 resonance energy transfer Methods 0.000 claims 4
- OPINTGHFESTVAX-BQBZGAKWSA-N Gln-Arg Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@H](C(O)=O)CCCN=C(N)N OPINTGHFESTVAX-BQBZGAKWSA-N 0.000 claims 3
- 101000652315 Homo sapiens Synaptosomal-associated protein 25 Proteins 0.000 claims 3
- 102000004183 Synaptosomal-Associated Protein 25 Human genes 0.000 claims 3
- 108010057722 Synaptosomal-Associated Protein 25 Proteins 0.000 claims 3
- 230000005281 excited state Effects 0.000 claims 3
- 102000053886 human SNAP25 Human genes 0.000 claims 3
- 238000004020 luminiscence type Methods 0.000 claims 3
- 230000003278 mimic effect Effects 0.000 claims 3
- 229940053031 botulinum toxin Drugs 0.000 claims 2
- 150000001413 amino acids Chemical group 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 239000013592 cell lysate Substances 0.000 claims 1
- 231100001102 clostridial toxin Toxicity 0.000 description 2
- 108010057266 Type A Botulinum Toxins Proteins 0.000 description 1
- 150000001408 amides Chemical group 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/942,098 US7332567B2 (en) | 2001-08-28 | 2001-08-28 | Fret protease assays for clostridial toxins |
| PCT/US2002/027212 WO2004031773A1 (en) | 2001-08-28 | 2002-08-22 | Fret protease assays for clostridial toxins |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008062974A Division JP2008174567A (ja) | 2001-08-28 | 2008-03-12 | クロストリジウム毒素のためのｆｒｅｔプロテアーゼアッセイ |
| JP2008062977A Division JP2008201786A (ja) | 2001-08-28 | 2008-03-12 | クロストリジウム毒素のためのｆｒｅｔプロテアーゼアッセイ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005520000A JP2005520000A (ja) | 2005-07-07 |
| JP2005520000A5 true JP2005520000A5 (https=) | 2008-05-01 |
Family
ID=27613894
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004541415A Pending JP2005520000A (ja) | 2001-08-28 | 2002-08-22 | クロストリジウム毒素のためのｆｒｅｔプロテアーゼアッセイ |
| JP2008062974A Pending JP2008174567A (ja) | 2001-08-28 | 2008-03-12 | クロストリジウム毒素のためのｆｒｅｔプロテアーゼアッセイ |
| JP2008062977A Pending JP2008201786A (ja) | 2001-08-28 | 2008-03-12 | クロストリジウム毒素のためのｆｒｅｔプロテアーゼアッセイ |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008062974A Pending JP2008174567A (ja) | 2001-08-28 | 2008-03-12 | クロストリジウム毒素のためのｆｒｅｔプロテアーゼアッセイ |
| JP2008062977A Pending JP2008201786A (ja) | 2001-08-28 | 2008-03-12 | クロストリジウム毒素のためのｆｒｅｔプロテアーゼアッセイ |
Country Status (9)
| Country | Link |
|---|---|
| US (10) | US7332567B2 (https=) |
| EP (2) | EP1438586B1 (https=) |
| JP (3) | JP2005520000A (https=) |
| AT (2) | ATE541213T1 (https=) |
| AU (1) | AU2002368109B8 (https=) |
| BR (1) | BRPI0212140B8 (https=) |
| CA (1) | CA2462686C (https=) |
| DK (2) | DK1438586T3 (https=) |
| WO (1) | WO2004031773A1 (https=) |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060141572A1 (en) * | 1993-09-21 | 2006-06-29 | Smith Leonard A | Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy |
| FR2809733B1 (fr) * | 2000-06-02 | 2004-04-30 | Inst Nat Sante Rech Med | Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants |
| US7354735B2 (en) * | 2001-05-24 | 2008-04-08 | Hong Kong University Of Science And Technology | Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis |
| US7374896B2 (en) | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
| US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
| US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
| US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
| US20060063221A1 (en) * | 2004-09-21 | 2006-03-23 | Williams Dudley J | Lanthanide-based substrates and methods for determining clostridial toxin activity |
| US20080064054A1 (en) * | 2001-08-28 | 2008-03-13 | Ester Fernandez-Salas | Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity |
| US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
| WO2004031355A2 (en) * | 2002-10-01 | 2004-04-15 | University Of Maryland | Methods for identifying inhibitors of botulinum neurotoxins |
| US20040115727A1 (en) * | 2002-12-11 | 2004-06-17 | Allergan, Inc., A Corporation | Evolved clostridial toxins with altered protease specificity |
| WO2005076785A2 (en) * | 2003-12-19 | 2005-08-25 | Wisconsin Alumni Research Foundation | Method and compositions for detecting botulinum neurotoxin |
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| US8617838B2 (en) * | 2004-09-20 | 2013-12-31 | University Of Massachusetts | Fluorescent proteins and related methods and compounds |
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| WO2006068794A2 (en) | 2004-11-22 | 2006-06-29 | New York University | Genetically engineered clostridial genes, proteins encoded by the engineered genes, and uses thereof |
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| EP2293064B1 (en) * | 2005-04-05 | 2012-04-04 | Allergan, Inc. | Lipophilic dye-based FRET assays for clostridial toxin activity |
| ATE518882T1 (de) * | 2005-09-19 | 2011-08-15 | Allergan Inc | Mit clostridientoxin aktivierbare clostridientoxine |
| CA2602972C (en) * | 2005-10-12 | 2013-09-24 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
| US8753831B2 (en) * | 2007-06-05 | 2014-06-17 | City Of Hope | Methods for detection of botulinum neurotoxin |
| WO2009035476A1 (en) | 2007-09-14 | 2009-03-19 | Biosentinel, Llc | Resonance energy transfer assay with cleavage sequence and spacer |
| KR101604515B1 (ko) | 2008-03-14 | 2016-03-17 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정 |
| PT2271670E (pt) | 2008-03-14 | 2014-11-28 | Allergan Inc | Ensaio imunobaseado da atividade do serotipo a da toxina botulínica |
| CN105833254A (zh) | 2008-12-10 | 2016-08-10 | 阿勒根公司 | 梭菌毒素药物组合物 |
| KR101923847B1 (ko) | 2009-03-13 | 2018-11-29 | 알러간, 인코포레이티드 | 면역 기반 재표적화된 엔도펩티다제 활성 검정 |
| BRPI1008940A2 (pt) | 2009-03-13 | 2020-10-27 | Allergan, Inc. | linhagem de célula clonal estabelecida suscetível à intoxicação por bont/a |
| EP2425251B1 (en) * | 2009-04-27 | 2017-10-25 | Merz Pharma GmbH & Co. KGaA | Means and methods for the determination of the amount of neurotoxin polypeptide and of its catalytic and proteolytic activities |
| US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
| EP2483666A1 (en) | 2009-10-02 | 2012-08-08 | Allergan, Inc. | In vitro method of determining changes in a protein environment |
| ES2676277T3 (es) * | 2009-10-16 | 2018-07-18 | Biomadison, Inc. | Células modificadas genéticamente para el ensayo de transferencia de energía por resonancia con el resto del sustrato de sinaptobrevina |
| EP2528941A4 (en) * | 2010-01-25 | 2013-05-29 | Univ New York | FOR TRANSPORT STUDIES AND NEURONAL ADMINISTRATION, MANIPULATED RECOMBINANT DERIVATIVES FROM BOTULINUM NEUROTOXINES |
| US8569254B2 (en) | 2010-12-10 | 2013-10-29 | National Yang Ming University | Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same |
| ES2653249T3 (es) | 2011-09-29 | 2018-02-06 | Cellsnap, Llc | Composiciones y métodos para ensayos de toxigenicidad |
| EP2798077A2 (en) | 2011-12-31 | 2014-11-05 | Allergan, Inc. | Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A |
| US8962340B2 (en) | 2012-12-03 | 2015-02-24 | Src, Inc. | Real-time assay for the detection of botulinum toxin |
| GB2512691B (en) * | 2012-12-03 | 2016-06-29 | Src Inc | Real-time assay for the detection of botulinum toxin |
| WO2014108480A1 (en) * | 2013-01-09 | 2014-07-17 | Friedrich-Alexander-Universitaet Erlangen-Nuernberg | Method for in vitro detection and monitoring of a disease by measuring disease-associated protease activity in extracellular vesicles |
| US9315549B2 (en) | 2013-01-28 | 2016-04-19 | New York University | Treatment methods using atoxic neurotoxin derivatives |
| AU2014301116B2 (en) | 2013-06-28 | 2019-11-14 | Merz Pharma Gmbh & Co. Kgaa | Means and methods for the determination of the biological activity of Neurotoxin polypeptides in cells |
| KR20170026624A (ko) | 2014-07-07 | 2017-03-08 | 알러간, 인코포레이티드 | 조직 샘플에서 절단된 snap25를 검출하는 방법 |
| PT3177640T (pt) | 2014-08-08 | 2020-08-31 | Univ Leland Stanford Junior | Agentes pd-1 de alta afinidade e métodos de utilização |
| AU2015301737B2 (en) * | 2014-08-12 | 2021-01-28 | Biomadison, Inc. | Botulinum neurotoxins with modified light chain specifity and methods for producing same |
| US11897921B2 (en) | 2014-12-09 | 2024-02-13 | New York University | Propeptide fusion comprising a mutated clostridium botulinum neurotoxin and a VHH domain |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| CN106117525B (zh) * | 2016-06-27 | 2018-01-05 | 温州医科大学 | pH敏感嵌段聚合物、FRET复合物及其制备方法 |
| KR102480965B1 (ko) | 2016-09-13 | 2022-12-26 | 알레간 인코포레이티드 | 안정화된 비단백질 클로스트리듐 독소 조성물 |
| WO2018073370A1 (en) * | 2016-10-21 | 2018-04-26 | Toxogen Gmbh | A functional detection assay devoid of antibodies for serotyping of botulinum neurotoxins |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11499965B2 (en) | 2017-02-08 | 2022-11-15 | Tokyo Institute Of Technology | Kit for detecting antigen or measuring its amount |
| GB201702500D0 (en) | 2017-02-16 | 2017-04-05 | Univ Sheffield | Stable vamp reporter assay |
| CN109211863B (zh) * | 2018-10-26 | 2021-04-20 | 大连民族大学 | 利用Eu2+f-f跃迁光谱检测爆炸物TNP的方法 |
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-
2001
- 2001-08-28 US US09/942,098 patent/US7332567B2/en not_active Expired - Lifetime
-
2002
- 2002-08-22 AU AU2002368109A patent/AU2002368109B8/en not_active Expired
- 2002-08-22 WO PCT/US2002/027212 patent/WO2004031773A1/en not_active Ceased
- 2002-08-22 BR BRPI0212140A patent/BRPI0212140B8/pt not_active IP Right Cessation
- 2002-08-22 DK DK02807745.1T patent/DK1438586T3/da active
- 2002-08-22 DK DK07023843.1T patent/DK1901069T3/da active
- 2002-08-22 EP EP02807745A patent/EP1438586B1/en not_active Revoked
- 2002-08-22 AT AT02807745T patent/ATE541213T1/de active
- 2002-08-22 JP JP2004541415A patent/JP2005520000A/ja active Pending
- 2002-08-22 CA CA2462686A patent/CA2462686C/en not_active Expired - Lifetime
- 2002-08-22 AT AT07023843T patent/ATE540317T1/de active
- 2002-08-22 EP EP07023843A patent/EP1901069B1/en not_active Revoked
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2007
- 2007-07-20 US US11/780,872 patent/US20080032318A1/en not_active Abandoned
- 2007-07-20 US US11/780,925 patent/US20080038756A1/en not_active Abandoned
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2008
- 2008-03-12 JP JP2008062974A patent/JP2008174567A/ja active Pending
- 2008-03-12 JP JP2008062977A patent/JP2008201786A/ja active Pending
- 2008-10-21 US US12/192,798 patent/US20090042231A1/en not_active Abandoned
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2009
- 2009-11-17 US US12/620,388 patent/US8048643B2/en not_active Expired - Fee Related
- 2009-11-17 US US12/620,337 patent/US20100075346A1/en not_active Abandoned
- 2009-11-17 US US12/620,370 patent/US8003753B2/en not_active Expired - Fee Related
- 2009-11-17 US US12/620,417 patent/US8053209B2/en not_active Expired - Fee Related
- 2009-11-17 US US12/620,434 patent/US8013113B2/en not_active Expired - Fee Related
- 2009-11-17 US US12/620,400 patent/US8053208B2/en not_active Expired - Fee Related
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