JP2008513038A5 - - Google Patents
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- Publication number
- JP2008513038A5 JP2008513038A5 JP2007533521A JP2007533521A JP2008513038A5 JP 2008513038 A5 JP2008513038 A5 JP 2008513038A5 JP 2007533521 A JP2007533521 A JP 2007533521A JP 2007533521 A JP2007533521 A JP 2007533521A JP 2008513038 A5 JP2008513038 A5 JP 2008513038A5
- Authority
- JP
- Japan
- Prior art keywords
- recognition sequence
- bont
- peptidomimetic
- clostridial toxin
- consecutive residues
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/948,097 US7399607B2 (en) | 2004-09-22 | 2004-09-22 | Fluorescence polarization assays for determining clostridial toxin activity |
| PCT/US2005/032328 WO2007001358A2 (en) | 2004-09-22 | 2005-09-09 | Fluorescence polarization assays for determining clostridial toxin activity |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012125033A Division JP2012210215A (ja) | 2004-09-22 | 2012-05-31 | クロストリジウム毒素活性の測定のための蛍光偏光アッセイ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008513038A JP2008513038A (ja) | 2008-05-01 |
| JP2008513038A5 true JP2008513038A5 (https=) | 2008-10-30 |
Family
ID=36074524
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007533521A Pending JP2008513038A (ja) | 2004-09-22 | 2005-09-09 | クロストリジウム毒素活性の測定のための蛍光偏光アッセイ |
| JP2012125033A Pending JP2012210215A (ja) | 2004-09-22 | 2012-05-31 | クロストリジウム毒素活性の測定のための蛍光偏光アッセイ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012125033A Pending JP2012210215A (ja) | 2004-09-22 | 2012-05-31 | クロストリジウム毒素活性の測定のための蛍光偏光アッセイ |
Country Status (11)
| Country | Link |
|---|---|
| US (7) | US7399607B2 (https=) |
| EP (2) | EP1807698B1 (https=) |
| JP (2) | JP2008513038A (https=) |
| KR (1) | KR101442246B1 (https=) |
| AT (1) | ATE472101T1 (https=) |
| AU (1) | AU2005333632B2 (https=) |
| CA (1) | CA2581231A1 (https=) |
| DE (1) | DE602005021981D1 (https=) |
| DK (1) | DK1807698T3 (https=) |
| ES (2) | ES2345780T3 (https=) |
| WO (1) | WO2007001358A2 (https=) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
| US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
| US7374896B2 (en) * | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
| US20080064054A1 (en) * | 2001-08-28 | 2008-03-13 | Ester Fernandez-Salas | Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity |
| US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
| US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
| US7399607B2 (en) * | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
| EP2293064B1 (en) * | 2005-04-05 | 2012-04-04 | Allergan, Inc. | Lipophilic dye-based FRET assays for clostridial toxin activity |
| CA2605160A1 (en) * | 2005-04-05 | 2006-10-05 | Allergan, Inc. | Clostridial toxin activity assays |
| ATE518882T1 (de) * | 2005-09-19 | 2011-08-15 | Allergan Inc | Mit clostridientoxin aktivierbare clostridientoxine |
| CA2602972C (en) * | 2005-10-12 | 2013-09-24 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
| US8003314B2 (en) | 2007-04-16 | 2011-08-23 | Diagnostic Hybrids, Inc. | Methods for direct fluorescent antibody virus detection in liquids |
| US8753831B2 (en) | 2007-06-05 | 2014-06-17 | City Of Hope | Methods for detection of botulinum neurotoxin |
| WO2009008595A1 (en) | 2007-07-10 | 2009-01-15 | Medy-Tox, Inc. | Pharmaceutical liquid composition of botulinum toxin with improved stability |
| KR101604515B1 (ko) * | 2008-03-14 | 2016-03-17 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 a 활성 검정 |
| PT2271670E (pt) | 2008-03-14 | 2014-11-28 | Allergan Inc | Ensaio imunobaseado da atividade do serotipo a da toxina botulínica |
| BRPI1008940A2 (pt) | 2009-03-13 | 2020-10-27 | Allergan, Inc. | linhagem de célula clonal estabelecida suscetível à intoxicação por bont/a |
| KR101923847B1 (ko) * | 2009-03-13 | 2018-11-29 | 알러간, 인코포레이티드 | 면역 기반 재표적화된 엔도펩티다제 활성 검정 |
| US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
| EP2483666A1 (en) | 2009-10-02 | 2012-08-08 | Allergan, Inc. | In vitro method of determining changes in a protein environment |
| EP2798077A2 (en) | 2011-12-31 | 2014-11-05 | Allergan, Inc. | Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A |
| LT2800973T (lt) | 2012-01-04 | 2018-09-10 | Biomadison, Inc. | Būdai ir junginiai, skirti botulino tyrimų jautrumo padidinimui |
| US8962340B2 (en) | 2012-12-03 | 2015-02-24 | Src, Inc. | Real-time assay for the detection of botulinum toxin |
| LT3030905T (lt) | 2013-08-09 | 2018-01-25 | Biomadison, Inc. | Padidinto jautrumo botulino toksino tyrimas |
| KR20170026624A (ko) | 2014-07-07 | 2017-03-08 | 알러간, 인코포레이티드 | 조직 샘플에서 절단된 snap25를 검출하는 방법 |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| FR3053689B1 (fr) * | 2016-07-06 | 2021-01-08 | Univ Claude Bernard Lyon | Polypeptides substrats des proteases de la famille des astacines et leurs utilisations |
| KR102480965B1 (ko) | 2016-09-13 | 2022-12-26 | 알레간 인코포레이티드 | 안정화된 비단백질 클로스트리듐 독소 조성물 |
| WO2018073370A1 (en) * | 2016-10-21 | 2018-04-26 | Toxogen Gmbh | A functional detection assay devoid of antibodies for serotyping of botulinum neurotoxins |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
Family Cites Families (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5804604A (en) * | 1989-12-21 | 1998-09-08 | Biogen, Inc. | Tat-derived transport polypeptides and fusion proteins |
| GB9411138D0 (en) * | 1994-06-03 | 1994-07-27 | Microbiological Res Authority | Toxin assay |
| US5962637A (en) * | 1994-06-03 | 1999-10-05 | Microbiological Research Authority | Toxin assay |
| US20010046668A1 (en) * | 1994-10-21 | 2001-11-29 | G.D. Searle & Co. | Fluorescence polarization method for determining protease activity |
| US5693476A (en) * | 1995-02-24 | 1997-12-02 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of screening for compounds capable of modulating vesicular release |
| GB9508204D0 (en) * | 1995-04-21 | 1995-06-07 | Speywood Lab Ltd | A novel agent able to modify peripheral afferent function |
| US5804395A (en) * | 1995-12-01 | 1998-09-08 | The United States Of America As Represented By The Secretary Of The Navy | Fluorescence polarization assays of enzymes and substrates therefore |
| US6803188B1 (en) * | 1996-01-31 | 2004-10-12 | The Regents Of The University Of California | Tandem fluorescent protein constructs |
| US6169074B1 (en) * | 1996-03-18 | 2001-01-02 | The Regents Of The University Of California | Peptide inhibitors of neurotransmitter secretion by neuronal cells |
| FI102922B (fi) * | 1996-06-28 | 1999-03-15 | Valtion Teknillinen | Fluoresenssiin perustuva immunomääritysmenetelmä |
| WO1998005962A1 (en) * | 1996-08-02 | 1998-02-12 | Panvera Corporation | A method for quantitating competitive binding of molecules to proteins utilizing fluorescence polarization |
| US5965699A (en) * | 1996-11-06 | 1999-10-12 | The United States Of America As Represented By The Secretary Of The Army | Assay for the proteolytic activity of serotype a from clostridium botulinum |
| US6197928B1 (en) | 1997-03-14 | 2001-03-06 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
| US6180340B1 (en) | 1997-10-31 | 2001-01-30 | Gen-Probe Incorporated | Extended dynamic range assays |
| WO1999029721A1 (en) | 1997-12-10 | 1999-06-17 | Washington University | Anti-pathogen system and methods of use thereof |
| JP2002507410A (ja) * | 1998-03-27 | 2002-03-12 | プロルーム・リミテッド | ルシフェラーゼ、蛍光タンパク質、ルシフェラーゼおよび蛍光タンパク質をコードする核酸および、診断、高処理スクリーニングおよび新規アイテムにおけるその使用 |
| US6251583B1 (en) * | 1998-04-27 | 2001-06-26 | Schering Corporation | Peptide substrates for HCV NS3 protease assays |
| EP1073763A4 (en) | 1998-04-28 | 2002-08-07 | Univ Washington | METHODS FOR TRANSDUCING FUSION MOLECULES |
| DE69941085D1 (de) * | 1998-07-17 | 2009-08-20 | Univ Maryland | Manipulierte proteine zum analyten nachweis |
| JP2002531113A (ja) | 1998-12-10 | 2002-09-24 | ワシントン大学 | 蛋白質の形質導入システムおよびその使用法 |
| US6469154B1 (en) | 1999-05-21 | 2002-10-22 | The Regents Of The University Of California | Fluorescent protein indicators |
| US6323039B1 (en) * | 1999-06-22 | 2001-11-27 | Mitokor | Compositions and methods for assaying subcellular conditions and processes using energy transfer |
| US7105310B1 (en) * | 2000-07-19 | 2006-09-12 | California Institute Of Technology | Detection of biomolecules by sensitizer-linked substrates |
| WO2001018038A2 (en) | 1999-08-20 | 2001-03-15 | Imperial College Innovations Limited | Isoforms of snare molecules and the uses thereof in modulation of cellular exocytosis |
| US6903187B1 (en) * | 2000-07-21 | 2005-06-07 | Allergan, Inc. | Leucine-based motif and clostridial neurotoxins |
| US20030219462A1 (en) * | 2000-07-21 | 2003-11-27 | Allergan Sales, Inc | Clostridial neurotoxin compositions and modified clostridial neurotoxins |
| AU2001279134A1 (en) * | 2000-07-31 | 2002-02-13 | Maxygen, Inc. | Nucleotide incorporating enzymes |
| DE60131468D1 (de) | 2000-09-25 | 2007-12-27 | U S Medical Res Inst Of Infect | Hoch-durchsatz assays für proteolytische aktivitäten von clostridium neurotoxinen |
| DE50212934D1 (de) * | 2001-01-26 | 2008-12-04 | Evotec Ag | Verfahren und mittel zur detektion enzymatischer spalt- und verknüpfungsreaktionen |
| US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
| US20060063221A1 (en) * | 2004-09-21 | 2006-03-23 | Williams Dudley J | Lanthanide-based substrates and methods for determining clostridial toxin activity |
| US7374896B2 (en) * | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
| US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
| US6504006B1 (en) * | 2001-10-12 | 2003-01-07 | Nancy Rose Shine | Substrate peptides and assays for detecting and measuring proteolytic activity of serotype A neurotoxin from clostridium botulinum |
| US7361478B2 (en) * | 2001-11-20 | 2008-04-22 | Allergan, Inc. | High-throughput screen for identifying selective persistent sodium channels channel blockers |
| WO2003065004A2 (en) * | 2002-01-29 | 2003-08-07 | Discoverx, Inc. | Enzyme activation protease assay |
| US20040038307A1 (en) * | 2002-05-10 | 2004-02-26 | Engeneos, Inc. | Unique recognition sequences and methods of use thereof in protein analysis |
| US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
| WO2004031355A2 (en) | 2002-10-01 | 2004-04-15 | University Of Maryland | Methods for identifying inhibitors of botulinum neurotoxins |
| US20040115727A1 (en) * | 2002-12-11 | 2004-06-17 | Allergan, Inc., A Corporation | Evolved clostridial toxins with altered protease specificity |
| US7452690B2 (en) * | 2003-01-28 | 2008-11-18 | Discoverx Corporation | Protease EFC cell surface fusion protein assay |
| US7504202B2 (en) * | 2003-03-28 | 2009-03-17 | The United States Of America As Represented By The Secretary Of The Navy | Rapid immunoassay of anthrax protective antigen in vaccine cultures and bodily fluids by fluorescence polarization |
| US7432070B2 (en) * | 2003-06-16 | 2008-10-07 | Pierce Biotechnology, Inc. | Homogenous assay for enzymatic activity |
| US7198928B2 (en) * | 2003-09-15 | 2007-04-03 | Allergan, Inc. | Human COX-1 alternatively spliced variants and methods of using same |
| US7408640B2 (en) * | 2003-11-05 | 2008-08-05 | The United States Of America As Represented By The Secretary Of The Navy | Fluorescence polarization instruments and methods for detection of exposure to biological materials by fluorescence polarization immunoassay of saliva, oral or bodily fluids |
| US7531317B2 (en) * | 2003-11-25 | 2009-05-12 | Wisconsin Alumni Research Foundation | Fluorescence polarization assay to detect protease cleavage |
| WO2005076785A2 (en) * | 2003-12-19 | 2005-08-25 | Wisconsin Alumni Research Foundation | Method and compositions for detecting botulinum neurotoxin |
| US20060024794A1 (en) * | 2004-07-30 | 2006-02-02 | Shengwen Li | Novel methods for production of di-chain botulinum toxin |
| US7399607B2 (en) * | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
| JP2008514240A (ja) * | 2004-10-01 | 2008-05-08 | インヴィトロジェン コーポレーション | 生体分子のインビトロ合成用の供給バッファー、系、及び方法 |
| CA2602972C (en) * | 2005-10-12 | 2013-09-24 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
-
2004
- 2004-09-22 US US10/948,097 patent/US7399607B2/en not_active Expired - Lifetime
-
2005
- 2005-09-09 DK DK05858107.5T patent/DK1807698T3/da active
- 2005-09-09 KR KR1020077009002A patent/KR101442246B1/ko not_active Expired - Fee Related
- 2005-09-09 AU AU2005333632A patent/AU2005333632B2/en not_active Ceased
- 2005-09-09 JP JP2007533521A patent/JP2008513038A/ja active Pending
- 2005-09-09 DE DE602005021981T patent/DE602005021981D1/de not_active Expired - Lifetime
- 2005-09-09 CA CA002581231A patent/CA2581231A1/en not_active Abandoned
- 2005-09-09 EP EP05858107A patent/EP1807698B1/en not_active Revoked
- 2005-09-09 WO PCT/US2005/032328 patent/WO2007001358A2/en not_active Ceased
- 2005-09-09 EP EP09160607A patent/EP2107112A1/en not_active Withdrawn
- 2005-09-09 ES ES05858107T patent/ES2345780T3/es not_active Expired - Lifetime
- 2005-09-09 AT AT05858107T patent/ATE472101T1/de not_active IP Right Cessation
-
2008
- 2008-05-21 US US12/124,412 patent/US7638294B2/en not_active Expired - Lifetime
- 2008-05-22 US US12/125,723 patent/US7632655B2/en not_active Expired - Lifetime
- 2008-05-22 US US12/125,235 patent/US7635574B2/en not_active Expired - Lifetime
- 2008-05-22 US US12/125,327 patent/US7674601B2/en not_active Expired - Lifetime
-
2009
- 2009-04-23 ES ES21203994T patent/ES2928863T5/es active Active
- 2009-10-22 US US12/604,059 patent/US20100081155A1/en not_active Abandoned
- 2009-10-22 US US12/604,138 patent/US20100081156A1/en not_active Abandoned
-
2012
- 2012-05-31 JP JP2012125033A patent/JP2012210215A/ja active Pending
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