DE602005021981D1 - Assay zur Bestimmung der Aktivität von Clostridien-Toxin mittels Kombination von FRET und Fluoreszenzpolarisation - Google Patents

Assay zur Bestimmung der Aktivität von Clostridien-Toxin mittels Kombination von FRET und Fluoreszenzpolarisation

Info

Publication number
DE602005021981D1
DE602005021981D1 DE602005021981T DE602005021981T DE602005021981D1 DE 602005021981 D1 DE602005021981 D1 DE 602005021981D1 DE 602005021981 T DE602005021981 T DE 602005021981T DE 602005021981 T DE602005021981 T DE 602005021981T DE 602005021981 D1 DE602005021981 D1 DE 602005021981D1
Authority
DE
Germany
Prior art keywords
clostridial toxin
activity
fluorescence polarization
determining
fret
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602005021981T
Other languages
German (de)
English (en)
Inventor
Dudley J Williams
Marcella A Gilmore
Lance E Steward
Marc Verhagen
Kei Roger Aoki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Allergan Inc
Original Assignee
Allergan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36074524&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE602005021981(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Allergan Inc filed Critical Allergan Inc
Publication of DE602005021981D1 publication Critical patent/DE602005021981D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Prostheses (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE602005021981T 2004-09-22 2005-09-09 Assay zur Bestimmung der Aktivität von Clostridien-Toxin mittels Kombination von FRET und Fluoreszenzpolarisation Expired - Lifetime DE602005021981D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/948,097 US7399607B2 (en) 2004-09-22 2004-09-22 Fluorescence polarization assays for determining clostridial toxin activity
PCT/US2005/032328 WO2007001358A2 (en) 2004-09-22 2005-09-09 Fluorescence polarization assays for determining clostridial toxin activity

Publications (1)

Publication Number Publication Date
DE602005021981D1 true DE602005021981D1 (de) 2010-08-05

Family

ID=36074524

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005021981T Expired - Lifetime DE602005021981D1 (de) 2004-09-22 2005-09-09 Assay zur Bestimmung der Aktivität von Clostridien-Toxin mittels Kombination von FRET und Fluoreszenzpolarisation

Country Status (11)

Country Link
US (7) US7399607B2 (https=)
EP (2) EP1807698B1 (https=)
JP (2) JP2008513038A (https=)
KR (1) KR101442246B1 (https=)
AT (1) ATE472101T1 (https=)
AU (1) AU2005333632B2 (https=)
CA (1) CA2581231A1 (https=)
DE (1) DE602005021981D1 (https=)
DK (1) DK1807698T3 (https=)
ES (2) ES2345780T3 (https=)
WO (1) WO2007001358A2 (https=)

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US20080064054A1 (en) * 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
EP2293064B1 (en) * 2005-04-05 2012-04-04 Allergan, Inc. Lipophilic dye-based FRET assays for clostridial toxin activity
CA2605160A1 (en) * 2005-04-05 2006-10-05 Allergan, Inc. Clostridial toxin activity assays
ATE518882T1 (de) * 2005-09-19 2011-08-15 Allergan Inc Mit clostridientoxin aktivierbare clostridientoxine
CA2602972C (en) * 2005-10-12 2013-09-24 Allergan, Inc. Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret)
US8003314B2 (en) 2007-04-16 2011-08-23 Diagnostic Hybrids, Inc. Methods for direct fluorescent antibody virus detection in liquids
US8753831B2 (en) 2007-06-05 2014-06-17 City Of Hope Methods for detection of botulinum neurotoxin
WO2009008595A1 (en) 2007-07-10 2009-01-15 Medy-Tox, Inc. Pharmaceutical liquid composition of botulinum toxin with improved stability
KR101604515B1 (ko) * 2008-03-14 2016-03-17 알러간, 인코포레이티드 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정
PT2271670E (pt) 2008-03-14 2014-11-28 Allergan Inc Ensaio imunobaseado da atividade do serotipo a da toxina botulínica
BRPI1008940A2 (pt) 2009-03-13 2020-10-27 Allergan, Inc. linhagem de célula clonal estabelecida suscetível à intoxicação por bont/a
KR101923847B1 (ko) * 2009-03-13 2018-11-29 알러간, 인코포레이티드 면역 기반 재표적화된 엔도펩티다제 활성 검정
US8129139B2 (en) 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
EP2483666A1 (en) 2009-10-02 2012-08-08 Allergan, Inc. In vitro method of determining changes in a protein environment
EP2798077A2 (en) 2011-12-31 2014-11-05 Allergan, Inc. Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A
LT2800973T (lt) 2012-01-04 2018-09-10 Biomadison, Inc. Būdai ir junginiai, skirti botulino tyrimų jautrumo padidinimui
US8962340B2 (en) 2012-12-03 2015-02-24 Src, Inc. Real-time assay for the detection of botulinum toxin
LT3030905T (lt) 2013-08-09 2018-01-25 Biomadison, Inc. Padidinto jautrumo botulino toksino tyrimas
KR20170026624A (ko) 2014-07-07 2017-03-08 알러간, 인코포레이티드 조직 샘플에서 절단된 snap25를 검출하는 방법
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
FR3053689B1 (fr) * 2016-07-06 2021-01-08 Univ Claude Bernard Lyon Polypeptides substrats des proteases de la famille des astacines et leurs utilisations
KR102480965B1 (ko) 2016-09-13 2022-12-26 알레간 인코포레이티드 안정화된 비단백질 클로스트리듐 독소 조성물
WO2018073370A1 (en) * 2016-10-21 2018-04-26 Toxogen Gmbh A functional detection assay devoid of antibodies for serotyping of botulinum neurotoxins
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria

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Also Published As

Publication number Publication date
US7674601B2 (en) 2010-03-09
US20080305509A1 (en) 2008-12-11
US7632655B2 (en) 2009-12-15
KR20070055609A (ko) 2007-05-30
AU2005333632A1 (en) 2007-01-04
ES2928863T5 (en) 2025-11-25
US7635574B2 (en) 2009-12-22
US20080293084A1 (en) 2008-11-27
US7399607B2 (en) 2008-07-15
US20080305510A1 (en) 2008-12-11
US20080293085A1 (en) 2008-11-27
EP1807698A2 (en) 2007-07-18
ES2928863T3 (es) 2022-11-23
AU2005333632B2 (en) 2011-12-22
EP1807698B1 (en) 2010-06-23
US20100081155A1 (en) 2010-04-01
WO2007001358A3 (en) 2007-06-28
US7638294B2 (en) 2009-12-29
CA2581231A1 (en) 2007-01-04
DK1807698T3 (da) 2010-09-20
US20060063222A1 (en) 2006-03-23
JP2008513038A (ja) 2008-05-01
ATE472101T1 (de) 2010-07-15
JP2012210215A (ja) 2012-11-01
KR101442246B1 (ko) 2014-10-01
WO2007001358A2 (en) 2007-01-04
ES2345780T3 (es) 2010-10-01
EP2107112A1 (en) 2009-10-07
US20100081156A1 (en) 2010-04-01

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