AU2005333632B2 - Fluorescence polarization assays for determining clostridial toxin activity - Google Patents

Fluorescence polarization assays for determining clostridial toxin activity Download PDF

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Publication number
AU2005333632B2
AU2005333632B2 AU2005333632A AU2005333632A AU2005333632B2 AU 2005333632 B2 AU2005333632 B2 AU 2005333632B2 AU 2005333632 A AU2005333632 A AU 2005333632A AU 2005333632 A AU2005333632 A AU 2005333632A AU 2005333632 B2 AU2005333632 B2 AU 2005333632B2
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AU
Australia
Prior art keywords
bont
residues
clostridial toxin
vamp
recognition sequence
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Ceased
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AU2005333632A
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English (en)
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AU2005333632A1 (en
Inventor
Kei Roger Aoki
Marcella A. Gilmore
Lance E. Steward
Marc Verhagen
Dudley J. Williams
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Allergan Inc
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Allergan Inc
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Application filed by Allergan Inc filed Critical Allergan Inc
Publication of AU2005333632A1 publication Critical patent/AU2005333632A1/en
Application granted granted Critical
Publication of AU2005333632B2 publication Critical patent/AU2005333632B2/en
Priority to AU2012201501A priority Critical patent/AU2012201501A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Prostheses (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AU2005333632A 2004-09-22 2005-09-09 Fluorescence polarization assays for determining clostridial toxin activity Ceased AU2005333632B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2012201501A AU2012201501A1 (en) 2004-09-22 2012-03-13 Fluorescence polarization assays for determining clostridial toxin activity

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/948,097 US7399607B2 (en) 2004-09-22 2004-09-22 Fluorescence polarization assays for determining clostridial toxin activity
US10/948,097 2004-09-22
PCT/US2005/032328 WO2007001358A2 (en) 2004-09-22 2005-09-09 Fluorescence polarization assays for determining clostridial toxin activity

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2012201501A Division AU2012201501A1 (en) 2004-09-22 2012-03-13 Fluorescence polarization assays for determining clostridial toxin activity

Publications (2)

Publication Number Publication Date
AU2005333632A1 AU2005333632A1 (en) 2007-01-04
AU2005333632B2 true AU2005333632B2 (en) 2011-12-22

Family

ID=36074524

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2005333632A Ceased AU2005333632B2 (en) 2004-09-22 2005-09-09 Fluorescence polarization assays for determining clostridial toxin activity

Country Status (11)

Country Link
US (7) US7399607B2 (https=)
EP (2) EP1807698B1 (https=)
JP (2) JP2008513038A (https=)
KR (1) KR101442246B1 (https=)
AT (1) ATE472101T1 (https=)
AU (1) AU2005333632B2 (https=)
CA (1) CA2581231A1 (https=)
DE (1) DE602005021981D1 (https=)
DK (1) DK1807698T3 (https=)
ES (2) ES2345780T3 (https=)
WO (1) WO2007001358A2 (https=)

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US7374896B2 (en) * 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US20080064054A1 (en) * 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
EP2293064B1 (en) * 2005-04-05 2012-04-04 Allergan, Inc. Lipophilic dye-based FRET assays for clostridial toxin activity
CA2605160A1 (en) * 2005-04-05 2006-10-05 Allergan, Inc. Clostridial toxin activity assays
ATE518882T1 (de) * 2005-09-19 2011-08-15 Allergan Inc Mit clostridientoxin aktivierbare clostridientoxine
CA2602972C (en) * 2005-10-12 2013-09-24 Allergan, Inc. Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret)
US8003314B2 (en) 2007-04-16 2011-08-23 Diagnostic Hybrids, Inc. Methods for direct fluorescent antibody virus detection in liquids
US8753831B2 (en) 2007-06-05 2014-06-17 City Of Hope Methods for detection of botulinum neurotoxin
WO2009008595A1 (en) 2007-07-10 2009-01-15 Medy-Tox, Inc. Pharmaceutical liquid composition of botulinum toxin with improved stability
KR101604515B1 (ko) * 2008-03-14 2016-03-17 알러간, 인코포레이티드 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정
PT2271670E (pt) 2008-03-14 2014-11-28 Allergan Inc Ensaio imunobaseado da atividade do serotipo a da toxina botulínica
BRPI1008940A2 (pt) 2009-03-13 2020-10-27 Allergan, Inc. linhagem de célula clonal estabelecida suscetível à intoxicação por bont/a
KR101923847B1 (ko) * 2009-03-13 2018-11-29 알러간, 인코포레이티드 면역 기반 재표적화된 엔도펩티다제 활성 검정
US8129139B2 (en) 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
EP2483666A1 (en) 2009-10-02 2012-08-08 Allergan, Inc. In vitro method of determining changes in a protein environment
EP2798077A2 (en) 2011-12-31 2014-11-05 Allergan, Inc. Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A
LT2800973T (lt) 2012-01-04 2018-09-10 Biomadison, Inc. Būdai ir junginiai, skirti botulino tyrimų jautrumo padidinimui
US8962340B2 (en) 2012-12-03 2015-02-24 Src, Inc. Real-time assay for the detection of botulinum toxin
LT3030905T (lt) 2013-08-09 2018-01-25 Biomadison, Inc. Padidinto jautrumo botulino toksino tyrimas
KR20170026624A (ko) 2014-07-07 2017-03-08 알러간, 인코포레이티드 조직 샘플에서 절단된 snap25를 검출하는 방법
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
FR3053689B1 (fr) * 2016-07-06 2021-01-08 Univ Claude Bernard Lyon Polypeptides substrats des proteases de la famille des astacines et leurs utilisations
KR102480965B1 (ko) 2016-09-13 2022-12-26 알레간 인코포레이티드 안정화된 비단백질 클로스트리듐 독소 조성물
WO2018073370A1 (en) * 2016-10-21 2018-04-26 Toxogen Gmbh A functional detection assay devoid of antibodies for serotyping of botulinum neurotoxins
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria

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Also Published As

Publication number Publication date
US7674601B2 (en) 2010-03-09
US20080305509A1 (en) 2008-12-11
DE602005021981D1 (de) 2010-08-05
US7632655B2 (en) 2009-12-15
KR20070055609A (ko) 2007-05-30
AU2005333632A1 (en) 2007-01-04
ES2928863T5 (en) 2025-11-25
US7635574B2 (en) 2009-12-22
US20080293084A1 (en) 2008-11-27
US7399607B2 (en) 2008-07-15
US20080305510A1 (en) 2008-12-11
US20080293085A1 (en) 2008-11-27
EP1807698A2 (en) 2007-07-18
ES2928863T3 (es) 2022-11-23
EP1807698B1 (en) 2010-06-23
US20100081155A1 (en) 2010-04-01
WO2007001358A3 (en) 2007-06-28
US7638294B2 (en) 2009-12-29
CA2581231A1 (en) 2007-01-04
DK1807698T3 (da) 2010-09-20
US20060063222A1 (en) 2006-03-23
JP2008513038A (ja) 2008-05-01
ATE472101T1 (de) 2010-07-15
JP2012210215A (ja) 2012-11-01
KR101442246B1 (ko) 2014-10-01
WO2007001358A2 (en) 2007-01-04
ES2345780T3 (es) 2010-10-01
EP2107112A1 (en) 2009-10-07
US20100081156A1 (en) 2010-04-01

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