JP2003506093A5 - - Google Patents

Info

Publication number

JP2003506093A5

JP2003506093A5 JP2001515842A JP2001515842A JP2003506093A5 JP 2003506093 A5 JP2003506093 A5 JP 2003506093A5 JP 2001515842 A JP2001515842 A JP 2001515842A JP 2001515842 A JP2001515842 A JP 2001515842A JP 2003506093 A5 JP2003506093 A5 JP 2003506093A5

Authority

JP

Japan

Prior art keywords

polypeptide

csfv

amino acid

fusion protein

pro

Prior art date

2000-08-07

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 229920001184 polypeptide Polymers 0.000 description 18
• 102000004196 processed proteins & peptides Human genes 0.000 description 18
• 108090000765 processed proteins & peptides Proteins 0.000 description 18
• 238000000034 method Methods 0.000 description 7
• 150000001413 amino acids Chemical group 0.000 description 6
• 230000000468 autoproteolytic effect Effects 0.000 description 6
• LXWYCLOUQZZDBD-LIYNQYRNSA-N csfv Chemical compound C([C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)[C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O)C1=CC=C(O)C=C1 LXWYCLOUQZZDBD-LIYNQYRNSA-N 0.000 description 6
• 108020001507 fusion proteins Proteins 0.000 description 6
• 102000037865 fusion proteins Human genes 0.000 description 6
• 210000004027 cell Anatomy 0.000 description 4
• 241000710778 Pestivirus Species 0.000 description 3
• 239000004365 Protease Substances 0.000 description 3
• 210000003000 inclusion body Anatomy 0.000 description 3
• 238000004519 manufacturing process Methods 0.000 description 3
• 108091005804 Peptidases Proteins 0.000 description 2
• 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
• 230000001580 bacterial effect Effects 0.000 description 2
• 239000013604 expression vector Substances 0.000 description 2
• 108020004707 nucleic acids Proteins 0.000 description 2
• 102000039446 nucleic acids Human genes 0.000 description 2
• 150000007523 nucleic acids Chemical class 0.000 description 2
• 241000710780 Bovine viral diarrhea virus 1 Species 0.000 description 1
• 108020004414 DNA Proteins 0.000 description 1
• 102000053602 DNA Human genes 0.000 description 1
• 241000588724 Escherichia coli Species 0.000 description 1
• 230000015572 biosynthetic process Effects 0.000 description 1
• PPBOKXIGFIBOGK-BDTUAEFFSA-N bvdv Chemical compound C([C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)C(C)C)[C@@H](C)CC)C1=CN=CN1 PPBOKXIGFIBOGK-BDTUAEFFSA-N 0.000 description 1
• 238000006243 chemical reaction Methods 0.000 description 1
• 238000003776 cleavage reaction Methods 0.000 description 1
• 238000012258 culturing Methods 0.000 description 1
• 238000007865 diluting Methods 0.000 description 1
• 230000001747 exhibiting effect Effects 0.000 description 1
• 239000006166 lysate Substances 0.000 description 1
• 239000013612 plasmid Substances 0.000 description 1
• 108010043393 protease N Proteins 0.000 description 1
• 230000007017 scission Effects 0.000 description 1
• 230000003381 solubilizing effect Effects 0.000 description 1