IL99611A - Small stabilized ILOC .E plasmids - Google Patents
Small stabilized ILOC .E plasmidsInfo
- Publication number
- IL99611A IL99611A IL9961184A IL9961184A IL99611A IL 99611 A IL99611 A IL 99611A IL 9961184 A IL9961184 A IL 9961184A IL 9961184 A IL9961184 A IL 9961184A IL 99611 A IL99611 A IL 99611A
- Authority
- IL
- Israel
- Prior art keywords
- plasmid
- sequence
- gene
- dna
- plasmids
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title claims description 347
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- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 10
- 102000013275 Somatomedins Human genes 0.000 description 10
- 229960000723 ampicillin Drugs 0.000 description 10
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 10
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- 239000006166 lysate Substances 0.000 description 2
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/73—Expression systems using phage (lambda) regulatory sequences
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Diabetes (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52196483A | 1983-08-10 | 1983-08-10 | |
| US06/636,727 US4710473A (en) | 1983-08-10 | 1984-08-06 | DNA plasmids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL99611A true IL99611A (en) | 1994-01-25 |
Family
ID=27060650
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL72642A IL72642A (en) | 1983-08-10 | 1984-08-10 | Circular dna plasmid for use as transformation vector |
| IL9961184A IL99611A (en) | 1983-08-10 | 1984-08-10 | Small stabilized ILOC .E plasmids |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL72642A IL72642A (en) | 1983-08-10 | 1984-08-10 | Circular dna plasmid for use as transformation vector |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US4710473A (de) |
| EP (1) | EP0136490B1 (de) |
| JP (1) | JPH0797991B2 (de) |
| CA (1) | CA1312026C (de) |
| DE (1) | DE3481716D1 (de) |
| IL (2) | IL72642A (de) |
| WO (1) | WO1985000829A1 (de) |
Families Citing this family (71)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1985000831A1 (en) * | 1983-08-10 | 1985-02-28 | Amgen | Microbial expression of insulin-like growth factor |
| KR850004274A (ko) * | 1983-12-13 | 1985-07-11 | 원본미기재 | 에리트로포이에틴의 제조방법 |
| NZ210501A (en) * | 1983-12-13 | 1991-08-27 | Kirin Amgen Inc | Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence |
| US4703008A (en) * | 1983-12-13 | 1987-10-27 | Kiren-Amgen, Inc. | DNA sequences encoding erythropoietin |
| US5126252A (en) * | 1984-08-27 | 1992-06-30 | Bio-Technology General Corp. | Expressions vectors containing Lambda PL promoter and T1 T2 RNA termination sequence, plasmids containing the vectors, hosts containing the plasmids and relatedmethods |
| US5081020A (en) * | 1984-08-27 | 1992-01-14 | Bio-Technology General Corp. | Expression vectors containing λPL promoter, T1 T2 rRNA termination sequence and origin of replication derived from a constitutive high copy number plasmid hosts and related methods |
| US5759816A (en) * | 1984-08-27 | 1998-06-02 | Bio-Technology General Corp. | Expression vectors containing λPL promoter and T1 T2 rRNA termination sequence plasmids containing the vectors hosts containing the plasmids and related methods |
| JP2594535B2 (ja) * | 1984-11-30 | 1997-03-26 | サントリー株式会社 | 高発現ベクターおよびその利用 |
| US4810643A (en) * | 1985-08-23 | 1989-03-07 | Kirin- Amgen Inc. | Production of pluripotent granulocyte colony-stimulating factor |
| JPS63500636A (ja) * | 1985-08-23 | 1988-03-10 | 麒麟麦酒株式会社 | 多分化能性顆粒球コロニー刺激因子をコードするｄｎａ |
| US6004548A (en) | 1985-08-23 | 1999-12-21 | Amgen, Inc. | Analogs of pluripotent granulocyte colony-stimulating factor |
| US5110731A (en) * | 1985-08-26 | 1992-05-05 | Amgen, Inc. | System for biotin synthesis |
| US4743679A (en) * | 1986-02-24 | 1988-05-10 | Creative Biomolecules, Inc. | Process for producing human epidermal growth factor and analogs thereof |
| JP2580303B2 (ja) * | 1987-02-19 | 1997-02-12 | ジ・アップジョン・カンパニー | 異種蛋白をコ−ド付けするｃＤＮＡ類、発現ベクタ−類および宿主類 |
| US5240837A (en) * | 1987-02-19 | 1993-08-31 | The Upjohn Company | CDNAS encoding somatotropin, expression vectors and hosts |
| US5222978A (en) | 1987-08-26 | 1993-06-29 | United States Surgical Corporation | Packaged synthetic absorbable surgical elements |
| US5472702A (en) * | 1987-08-26 | 1995-12-05 | United States Surgical Corporation | Sterilization of growth factors |
| US5366081A (en) | 1987-08-26 | 1994-11-22 | United States Surgical Corporation | Packaged synthetic absorbable surgical elements |
| US4885252A (en) * | 1987-09-08 | 1989-12-05 | Eli Lilly And Company | Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from aspergillus nidulans |
| US5599690A (en) * | 1988-02-01 | 1997-02-04 | Amgen Inc. | Control of norleucine incorporation into recombinant proteins |
| JP2877509B2 (ja) | 1989-05-19 | 1999-03-31 | アムジエン・インコーポレーテツド | メタロプロテイナーゼ阻害剤 |
| US5714465A (en) * | 1989-05-19 | 1998-02-03 | Amgen Inc. | Method of inhibiting tumor cell dissemination with a metalloproteinase inhibitor |
| US5359831A (en) | 1989-08-01 | 1994-11-01 | United States Surgical Corporation | Molded suture retainer |
| US5434135A (en) * | 1990-08-02 | 1995-07-18 | Indu Parikh | Growth factor compositions, preparation and use |
| IL99100A0 (en) * | 1990-08-13 | 1992-07-15 | Lilly Co Eli | Improved bacteriophage lambda pl promoters |
| WO1992006186A1 (en) * | 1990-09-28 | 1992-04-16 | Amgen Inc. | Cloning cartridges and expression vectors in gram-negative bacteria |
| CA2057715A1 (en) * | 1990-12-19 | 1992-06-20 | Charles L. Hershberger | Method for enhancing the structural stability of recombinant dna expression vectors |
| CA2059245C (en) * | 1991-02-08 | 2004-07-06 | Michael P. Chesterfield | Method and apparatus for calendering and coating/filling sutures |
| US5395761A (en) * | 1991-11-27 | 1995-03-07 | Eli Lilly And Company | Plasmid pHKY334, an expression vector for EK-BGH and host cells transformed therewith |
| IL107642A0 (en) * | 1992-11-20 | 1994-02-27 | Amgen Inc | Progenitor b cell stimulating factor |
| US5407810A (en) * | 1993-08-20 | 1995-04-18 | Genentech, Inc. | Aqueous multiple-phase isolation of polypeptide |
| US5663304A (en) * | 1993-08-20 | 1997-09-02 | Genentech, Inc. | Refolding of misfolded insulin-like growth factor-I |
| US6562596B1 (en) * | 1993-10-06 | 2003-05-13 | Amgen Inc. | Tissue inhibitor of metalloproteinase type three (TIMP-3) composition and methods |
| US5446024A (en) * | 1993-12-17 | 1995-08-29 | Genentech, Inc. | Purification of insulin-like growth factor |
| US5574008A (en) | 1994-08-30 | 1996-11-12 | Eli Lilly And Company | Biologically active fragments of glucagon-like insulinotropic peptide |
| IL117175A (en) * | 1995-02-20 | 2005-11-20 | Sankyo Co | Osteoclastogenesis inhibitory factor protein |
| US5904716A (en) * | 1995-04-26 | 1999-05-18 | Gendler; El | Method for reconstituting cartilage tissue using demineralized bone and product thereof |
| US7632922B1 (en) * | 1995-12-22 | 2009-12-15 | Amgen, Inc. | Osteoprotegerin |
| US20030207827A1 (en) * | 1995-12-22 | 2003-11-06 | William J. Boyle | Osteoprotegerin |
| US6369027B1 (en) | 1995-12-22 | 2002-04-09 | Amgen Inc. | Osteoprotegerin |
| US20050147611A1 (en) * | 1995-12-22 | 2005-07-07 | Amgen Inc. | Combination therapy for conditions leading to bone loss |
| IL129843A0 (en) * | 1996-11-08 | 2000-02-29 | Cytel Corp | Improved expression vectors |
| CZ300296B6 (cs) * | 1996-11-15 | 2009-04-15 | Genentech, Inc. | Zpusob izolace rekombinantního lidského neurotrofinu, neurotrofinový prostredek a zpusob cištení neurotrofinu |
| DE69738841D1 (de) | 1996-12-23 | 2008-08-28 | Immunex Corp | Ligand für rezeptor aktivator of nf-kappa b, ligand ist mitglied der tnf superfamilie |
| KR100496063B1 (ko) * | 1997-04-15 | 2005-06-21 | 산쿄 가부시키가이샤 | 신규단백질 및 그 제조방법 |
| DK0975754T4 (en) | 1997-04-16 | 2016-03-21 | Amgen Inc | Osteoprotegerin binding proteins and their receptors |
| US6316408B1 (en) * | 1997-04-16 | 2001-11-13 | Amgen Inc. | Methods of use for osetoprotegerin binding protein receptors |
| US6265542B1 (en) | 1997-10-24 | 2001-07-24 | Genentech, Inc. | Purification of molecules |
| EP1140136B1 (de) * | 1999-09-03 | 2007-05-16 | Amgen Inc. | Verfahren und zusammensetzungen zur prevention oder behandlung von krebs und von krebs assoziertem knochenverlust |
| US7223559B2 (en) * | 2000-04-06 | 2007-05-29 | Kyowa Hakko Kogyo Co., Ltd. | Plasmids for chromosomal recombination of Escherichia coli |
| US6780405B1 (en) * | 2000-04-28 | 2004-08-24 | Avant Immunotherapeutics, Inc. | Regulated antigen delivery system (RADS) |
| KR20030046411A (ko) * | 2000-09-08 | 2003-06-12 | 그리폰 테라퓨틱스, 인코포레이티드 | '위'-천연 화학적 라이게이션 |
| ATE426674T1 (de) | 2000-11-03 | 2009-04-15 | Genentech Inc | Stoffwechselanderungen in garungen zur produktion rekombinanter proteine |
| EP2292655B1 (de) | 2001-05-11 | 2012-03-14 | Amgen SF, LLC | Peptide und damit zusammenhängende an Tall-1 bindende Moleküle |
| US7138370B2 (en) | 2001-10-11 | 2006-11-21 | Amgen Inc. | Specific binding agents of human angiopoietin-2 |
| JP4761710B2 (ja) | 2002-04-05 | 2011-08-31 | アムジェン インコーポレイテッド | 選択的ｏｐｇｌ経路インヒビターとしてのヒト抗ｏｐｇｌ中和抗体 |
| US6919426B2 (en) | 2002-09-19 | 2005-07-19 | Amgen Inc. | Peptides and related molecules that modulate nerve growth factor activity |
| EP1684791A4 (de) | 2003-10-27 | 2009-07-01 | Amgen Inc | Zusammensetzungen und verfahren zur modulierung einer immunantwort auf ein immunogenes therapeutisches mittel |
| ME00226B (me) | 2004-07-15 | 2011-02-10 | Medarex Llc | Humana anti-ngf neutrališuća antitijela kao selektivni inhibitori ngf signalne kaskade |
| ES2446416T3 (es) * | 2005-12-09 | 2014-03-07 | Amgen, Inc. | Compuestos basados en quinolona que presentan actividad inhibidora de prolil hidroxilasa, composiciones y usos de los mismos |
| NZ568809A (en) | 2005-12-22 | 2011-08-26 | Genentech Inc | Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents |
| AR062069A1 (es) | 2006-07-14 | 2008-10-15 | Genentech Inc | Replegado de proteinas recombinantes |
| US7635715B2 (en) * | 2006-12-18 | 2009-12-22 | Amgen Inc. | Naphthalenone compounds exhibiting prolyl hydroxylase inhibitory activity, compositions, and uses thereof |
| ES2393326T3 (es) * | 2006-12-18 | 2012-12-20 | Amgen, Inc | Compuestos basados en azaquinolona que presentan actividad inhibidora de prolil hidroxilasas, composiciones y usos de los mismos |
| ES2442847T3 (es) * | 2007-04-18 | 2014-02-13 | Amgen, Inc | Quinolonas y azaquinolonas que inhiben la prolil hidroxilasa |
| ES2446418T3 (es) * | 2007-04-18 | 2014-03-07 | Amgen, Inc | Derivados de indanona que inhiben la prolil hidroxilasa |
| WO2008137060A1 (en) * | 2007-05-04 | 2008-11-13 | Amgen Inc. | Thienopyridine and thiazolopyridine derivatives that inhibit prolyl hydroxylase activity |
| AU2008248165B2 (en) * | 2007-05-04 | 2011-12-08 | Amgen Inc. | Diazaquinolones that inhibit prolyl hydroxylase activity |
| US20090192554A1 (en) | 2008-01-29 | 2009-07-30 | Confluent Surgical, Inc. | Bioabsorbable block copolymer |
| US8748097B1 (en) | 2011-12-02 | 2014-06-10 | President And Fellows Of Harvard College | Identification of agents for treating calcium disorders and uses thereof |
| CN115885045A (zh) * | 2020-06-17 | 2023-03-31 | 巴斯夫欧洲公司 | 质粒拷贝数调节和整合 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2759053A1 (de) * | 1977-12-30 | 1979-07-12 | Uhlin | Verfahren zum herstellen von genprodukten von plasmid-dns |
| FI793884A (fi) * | 1978-12-26 | 1980-06-27 | Cetus Corp | Stabila plasmider med stort kopieantal |
| US4374927A (en) * | 1981-02-24 | 1983-02-22 | The Board Of Trustees Of The Leland Stanford Jr. University | Extrachromosomal regulation of expression |
| CA1198067A (en) * | 1981-02-27 | 1985-12-17 | David H. Gelfand | Stable high copy number plasmids |
| EP0108132A1 (de) * | 1982-05-06 | 1984-05-16 | Applied Molecular Genetics Inc. | Herstellung und expression von genen für urogastron und dessen polypeptidanalogen |
| JPS59501852A (ja) * | 1982-09-16 | 1984-11-08 | アムジエン | 鳥類の成長ホルモン |
| DK410783D0 (da) * | 1982-09-16 | 1983-09-09 | Benzon A Salfred | Fremgangsmade til stabilisering af plasmider |
| CA1209501A (en) * | 1982-09-16 | 1986-08-12 | Nikos Panayotatos | Expression vector |
| AU569043B2 (en) * | 1982-09-16 | 1988-01-21 | Benzon Pharma A/S | Plasmids with conditional uncontrolled replication behaviour |
| NL8203716A (nl) * | 1982-09-24 | 1984-04-16 | Tno | Dna en plasmiden. |
| GB8308236D0 (en) * | 1983-03-25 | 1983-05-05 | Celltech Ltd | Dna vectors |
-
1984
- 1984-08-06 US US06/636,727 patent/US4710473A/en not_active Expired - Lifetime
- 1984-08-09 JP JP59503110A patent/JPH0797991B2/ja not_active Expired - Lifetime
- 1984-08-09 WO PCT/US1984/001251 patent/WO1985000829A1/en unknown
- 1984-08-10 EP EP84109538A patent/EP0136490B1/de not_active Expired
- 1984-08-10 CA CA000460748A patent/CA1312026C/en not_active Expired - Lifetime
- 1984-08-10 DE DE8484109538T patent/DE3481716D1/de not_active Expired - Lifetime
- 1984-08-10 IL IL72642A patent/IL72642A/xx not_active IP Right Cessation
- 1984-08-10 IL IL9961184A patent/IL99611A/en not_active IP Right Cessation
-
1994
- 1994-05-16 US US08/243,604 patent/US5674746A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO1985000829A1 (en) | 1985-02-28 |
| US5674746A (en) | 1997-10-07 |
| IL72642A0 (en) | 1984-11-30 |
| JPS60501988A (ja) | 1985-11-21 |
| EP0136490B1 (de) | 1990-03-21 |
| EP0136490A3 (en) | 1985-10-09 |
| JPH0797991B2 (ja) | 1995-10-25 |
| IL72642A (en) | 1993-04-04 |
| US4710473A (en) | 1987-12-01 |
| EP0136490A2 (de) | 1985-04-10 |
| DE3481716D1 (de) | 1990-04-26 |
| CA1312026C (en) | 1992-12-29 |
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