IL240616B - Methods for the generation of hepatocytes and cholangiocytes from multipotential gas cells - Google Patents

Methods for the generation of hepatocytes and cholangiocytes from multipotential gas cells

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Publication number
IL240616B
IL240616B IL240616A IL24061615A IL240616B IL 240616 B IL240616 B IL 240616B IL 240616 A IL240616 A IL 240616A IL 24061615 A IL24061615 A IL 24061615A IL 240616 B IL240616 B IL 240616B
Authority
IL
Israel
Prior art keywords
cholangiocytes
methods
stem cells
pluripotent stem
generating hepatocytes
Prior art date
Application number
IL240616A
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English (en)
Hebrew (he)
Other versions
IL240616A0 (en
Original Assignee
Univ Health Network
Hospital For Sick Children
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Filing date
Publication date
Application filed by Univ Health Network, Hospital For Sick Children filed Critical Univ Health Network
Publication of IL240616A0 publication Critical patent/IL240616A0/en
Publication of IL240616B publication Critical patent/IL240616B/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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    • C12N5/0679—Cells of the gastro-intestinal tract
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
IL240616A 2013-02-18 2015-08-17 Methods for the generation of hepatocytes and cholangiocytes from multipotential gas cells IL240616B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361766002P 2013-02-18 2013-02-18
PCT/CA2014/000122 WO2014124527A1 (en) 2013-02-18 2014-02-18 Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells

Publications (2)

Publication Number Publication Date
IL240616A0 IL240616A0 (en) 2015-10-29
IL240616B true IL240616B (en) 2021-07-29

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Family Applications (2)

Application Number Title Priority Date Filing Date
IL240616A IL240616B (en) 2013-02-18 2015-08-17 Methods for the generation of hepatocytes and cholangiocytes from multipotential gas cells
IL284523A IL284523A (en) 2013-02-18 2021-06-30 Methods for generating hepatocytes and cholangiocytes from multipotential gas cells

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IL284523A IL284523A (en) 2013-02-18 2021-06-30 Methods for generating hepatocytes and cholangiocytes from multipotential gas cells

Country Status (12)

Country Link
US (2) US20160002595A1 (ko)
EP (1) EP2956540A4 (ko)
JP (3) JP2016517266A (ko)
KR (5) KR20230019992A (ko)
CN (2) CN113913366A (ko)
AU (1) AU2014218290B2 (ko)
CA (1) CA2901377C (ko)
HK (1) HK1218137A1 (ko)
IL (2) IL240616B (ko)
SG (2) SG11201506520TA (ko)
WO (1) WO2014124527A1 (ko)
ZA (1) ZA201506189B (ko)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE049976T2 (hu) 2005-12-28 2020-11-30 Vertex Pharma N-[2,4-bisz(1,1-dimetil-etil)-5-hidroxi-fenil]-1,4-dihidro-4-oxo-kinolin-3-karboxamid amorf alakjának gyógyászati kompozíciói
JP5458112B2 (ja) 2009-02-03 2014-04-02 コーニンクレッカ ネザーランド アカデミー ヴァン ウェテンシャッペン 上皮幹細胞および該幹細胞を含むオルガノイドのための培養培地
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
SG11201506520TA (en) * 2013-02-18 2015-09-29 Univ Health Network Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells
WO2015020234A1 (ja) 2013-08-06 2015-02-12 武田薬品工業株式会社 ドパミン神経細胞の製造方法
CN104694456B (zh) * 2013-12-06 2018-05-29 中国科学院上海药物研究所 体外培养肝样细胞的方法及采用该方法培养的优化后肝样细胞
US10597633B2 (en) 2014-05-16 2020-03-24 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for organoids
CN107075467B (zh) * 2014-09-19 2020-08-21 新加坡科技研究局 由干细胞分化肝细胞样细胞
CN107250113B (zh) 2014-10-07 2019-03-29 弗特克斯药品有限公司 囊性纤维化跨膜传导调节蛋白的调节剂的共晶
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
JP5777127B1 (ja) * 2014-12-09 2015-09-09 公立大学法人横浜市立大学 原始腸内胚葉細胞及びその作製方法
RS58070B2 (sr) * 2015-02-20 2022-10-31 Inst Nat Sante Rech Med Upotreba laminina za diferencijaciju pluripotentnih ćelija u ćelije hepatocitne linije
WO2016183231A1 (en) 2015-05-12 2016-11-17 Baker Group, LLP Method and system for a bioartificial organ
GB201510950D0 (en) * 2015-06-22 2015-08-05 Cambridge Entpr Ltd In vitro Production of Cholangiocytes
KR20180055901A (ko) 2015-10-05 2018-05-25 오리그3엔, 인코포레이티드 간 기능장애의 확인 및 개선에 기반한 파킨슨병의 진단 및 치료
WO2017070145A1 (en) * 2015-10-19 2017-04-27 Cellular Dynamics International, Inc. Production of virus-receptive pluripotent stem cell (psc)-derived hepatocytes
CN106754636B (zh) * 2015-11-19 2019-08-30 中国人民解放军第二军医大学 体外诱导原代肝细胞胆管化并长期培养、扩增和分化的方法及其应用
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
GB201610748D0 (en) * 2016-06-20 2016-08-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved diffrentation method
US11116799B2 (en) * 2016-07-14 2021-09-14 Wisconsin Alumni Research Foundation Generation of uniform hepatocytes from human embryonic stem cells by inhibiting TGF-beta and methods of maintaining hepatic cultures
WO2018097127A1 (ja) * 2016-11-22 2018-05-31 国立大学法人京都大学 肝芽細胞から胆管上皮前駆細胞への段階的誘導方法
CA3043509A1 (en) * 2016-11-23 2018-05-31 Massimiliano PAGANELLI Encapsulated liver tissue
WO2018152120A1 (en) 2017-02-14 2018-08-23 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Methods of engineering human induced pluripotent stem cells to produce liver tissue
CN108660156A (zh) * 2017-03-16 2018-10-16 中国人民解放军军事科学院军事医学研究院 Cps1报告基因干细胞及其构建方法与应用
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
CN108865969B (zh) * 2017-05-11 2022-04-01 北京大学 Mapk/pkc信号通路激活剂促进人类胆管细胞分化和成熟
US10633907B2 (en) 2017-06-06 2020-04-28 Gto Access Systems, Llc Edge sensor for movable barrier
JP2020533025A (ja) * 2017-09-12 2020-11-19 ベス イスラエル デアコネス メディカル センター インコーポレイティッド 肝疾患および機能不全を処置するための組成物および方法
EP3702444A4 (en) * 2017-10-12 2021-06-02 Tokyo Institute of Technology METHOD FOR INDUCING THE DIFFERENTIATION OF PLURIPOTENT STEM CELLS IN HEPATOCYTE
CN107904207A (zh) * 2017-11-13 2018-04-13 广东艾时代生物科技有限责任公司 一种利用三维培养系统诱导人诱导性多能干细胞获得成熟肝细胞的方法
CN108004201B (zh) * 2017-12-07 2021-06-04 香港大学深圳医院 一种成熟的多能干细胞来源肝脏细胞的获取方法
US20230026916A1 (en) * 2018-07-05 2023-01-26 Active Genomes Expressed Diagnostics, Inc Viral Oncogene Influences and Gene Expression Patterns as Indicators of Early Tumorigenesis
US11566229B2 (en) 2018-08-31 2023-01-31 Wisconsin Alumni Research Foundation Expansion and maintenance of adult primary human hepatocytes in culture
CN109337858B (zh) * 2018-09-20 2022-03-15 中国人民解放军第二军医大学 用于乙肝病毒感染的原代肝细胞来源的肝前体样细胞模型、制备方法及应用
CN117802031A (zh) * 2018-09-30 2024-04-02 中国科学院分子细胞科学卓越创新中心 肝细胞的体外扩增培养方法及应用
US20210395679A1 (en) * 2018-11-09 2021-12-23 Children's Hospital Medical Center In vitro cell culture system for producing hepatocyte-like cells and uses thereof
CN111304147A (zh) * 2018-12-11 2020-06-19 中国科学院分子细胞科学卓越创新中心 利用内胚层干细胞规模化制备功能性胆管细胞的方法及其应用
KR102146932B1 (ko) * 2018-12-18 2020-08-21 한양대학교 산학협력단 간 줄기세포의 담관 세포로의 분화 유도용 배지 조성물 및 이의 용도
CN111500525B (zh) * 2019-01-30 2023-05-02 中国科学院广州生物医药与健康研究院 一种组合物及其应用
CN113423818A (zh) * 2019-02-26 2021-09-21 北京大学 长期培养肝细胞的组合物和方法
CN111849859B (zh) * 2019-04-04 2023-04-07 中国科学院分子细胞科学卓越创新中心 一种经基因编辑的功能性肝实质细胞的制备方法及其应用
US20220220440A1 (en) 2019-05-09 2022-07-14 FUJIFILM Cellular Dynamics, Inc. Methods for the production of hepatocytes
BR112021023389A2 (pt) * 2019-05-22 2022-02-01 Cleveland Clinic Found Geração de células de endoderma de intestino anterior dorsal e domínio anterior
US20220233605A1 (en) * 2019-06-04 2022-07-28 University Health Network Methods of making and using liver cells
CN110373380B (zh) * 2019-06-14 2022-01-28 中国科学院生态环境研究中心 一种肝脏类器官模型及其建立方法和应用
KR102275911B1 (ko) 2019-08-27 2021-07-12 (주)유앤아이씨 모바일 앱 가상화 기술과 비콘을 이용한 가변형 매장 관리 시스템
CN111004770B (zh) * 2019-09-25 2022-08-23 中国人民解放军军事科学院军事医学研究院 功能性肝细胞诱导方法及其专用三维诱导培养基和应用
CN113151147B (zh) * 2020-01-23 2023-07-25 北京大学 功能性肝实质细胞及其制备方法
US20230383261A1 (en) * 2020-10-14 2023-11-30 Sana Biotechnology, Inc. Hepatocyte-like cells
CN115216436A (zh) * 2021-04-16 2022-10-21 中国科学院分子细胞科学卓越创新中心 利用多能干细胞建立具备双向分化潜能的肝干细胞系的方法及其应用
US20230014010A1 (en) 2021-06-23 2023-01-19 Crispr Therapeutics Ag Engineered cells with improved protection from natural killer cell killing
CN113667603B (zh) * 2021-08-13 2023-12-22 合肥燃音生物科技有限公司 一种肝脏类器官培养芯片及其制备方法与应用
CN114085805B (zh) * 2021-11-23 2024-05-24 中山大学附属第三医院（中山大学肝脏病医院） 冬眠动物干细胞分化为肝细胞的方法、利用该肝细胞筛选人体外细胞保护的代谢产物的方法
CN114181893B (zh) * 2022-02-09 2022-09-13 天津外泌体科技有限公司 肝脏双表型细胞的成熟方法
CN114149961B (zh) * 2022-02-09 2022-04-22 天九再生医学(天津)科技有限公司 一种多谱系肝脏类器官及其构建方法和应用
CN114874973B (zh) * 2022-04-24 2023-03-21 深圳市三启生物技术有限公司 获得成熟肝细胞的培养基及培养方法
CN115011550B (zh) * 2022-07-14 2024-04-26 广东乾晖生物科技有限公司 一种用于悬浮培养条件下诱导肝细胞分化成熟的诱导培养基以及方法
CN115851578B (zh) * 2022-12-23 2023-11-21 华南理工大学 一种3d悬浮诱导持续扩增肝祖细胞类器官和/或肝细胞类器官的试剂盒及其应用
CN118667753A (zh) * 2023-03-17 2024-09-20 上海交通大学 制备成熟卵母细胞的方法及应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101627112A (zh) * 2006-06-28 2010-01-13 堪萨斯大学 将来自脐带基质的干细胞分化为肝细胞谱系细胞
WO2009013254A1 (en) * 2007-07-20 2009-01-29 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts stem cells
JP5560270B2 (ja) * 2008-07-08 2014-07-23 オンコメッド ファーマシューティカルズ インコーポレイテッド Ｎｏｔｃｈ結合剤およびアンタゴニストならびにその使用方法
WO2010049752A1 (en) * 2008-10-31 2010-05-06 Katholieke Universiteit Leuven Optimized methods for differentiation of cells into cells with hepatocyte and hepatocyte progenitor phenotypes, cells produced by the methods, and methods for using the cells
US20100329986A1 (en) * 2009-01-06 2010-12-30 Greenbaum Linda E Adult Hepatic Progenitor Cells and Methods of Use Thereof
CN102459574B (zh) * 2009-06-18 2016-06-29 塞拉提斯股份公司 用于人多潜能干(hPS)细胞的生长和分化的3D培养系统
US20120190059A1 (en) * 2009-07-23 2012-07-26 Beijing Huayuanbochuang Technology Co., Ltd. Methods for obtaining hepatocytes, hepatic endoderm cells and hepatic progenitor cells by induced differentiation
JP5968871B2 (ja) * 2010-04-13 2016-08-10 セルラー ダイナミクス インターナショナル， インコーポレイテッド フォワードプログラミングによる肝細胞の産生
US9127255B2 (en) * 2010-06-11 2015-09-08 Takara Bio Europe Ab 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes
WO2012178215A1 (en) * 2011-06-23 2012-12-27 The Children's Hospital Of Philadelphia Self-renewing endodermal progenitor lines generated from human pluripotent stem cells and methods of use thereof
SG11201506520TA (en) 2013-02-18 2015-09-29 Univ Health Network Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells

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