HUE019019T5 - Methods for Using miRNA for In vivo Cell Death Detection - Google Patents
Methods for Using miRNA for In vivo Cell Death Detection Download PDFInfo
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- HUE019019T5 HUE019019T5 HUE08795523A HUE08795523A HUE019019T5 HU E019019 T5 HUE019019 T5 HU E019019T5 HU E08795523 A HUE08795523 A HU E08795523A HU E08795523 A HUE08795523 A HU E08795523A HU E019019 T5 HUE019019 T5 HU E019019T5
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- mirna
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- cell death
- tissue
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| EP3433381B1 (en) | 2016-03-21 | 2024-07-24 | Diamir, LLC | Methods of using mirnas from bodily fluids for detection and differentiation of neurodegenerative diseases |
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| US11001891B2 (en) | 2016-11-14 | 2021-05-11 | Berg Llc | Methods for treating Parkinson's disease |
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| US10907211B1 (en) | 2017-02-16 | 2021-02-02 | Quantgene Inc. | Methods and compositions for detecting cancer biomarkers in bodily fluids |
| US10781487B2 (en) | 2017-07-24 | 2020-09-22 | Diamir, Llc | miRNA-based methods for detecting and monitoring aging |
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| GB2615975B (en) | 2019-02-14 | 2023-11-29 | Mirvie Inc | Methods and systems for determining a pregnancy-related state of a subject |
| WO2021085505A1 (ja) * | 2019-10-29 | 2021-05-06 | 国立研究開発法人 国立循環器病研究センター | 脳梗塞治療支援システム |
| US11211144B2 (en) | 2020-02-18 | 2021-12-28 | Tempus Labs, Inc. | Methods and systems for refining copy number variation in a liquid biopsy assay |
| US11211147B2 (en) | 2020-02-18 | 2021-12-28 | Tempus Labs, Inc. | Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing |
| US11475981B2 (en) | 2020-02-18 | 2022-10-18 | Tempus Labs, Inc. | Methods and systems for dynamic variant thresholding in a liquid biopsy assay |
Family Cites Families (27)
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| US5168039A (en) | 1990-09-28 | 1992-12-01 | The Board Of Trustees Of The University Of Arkansas | Repetitive DNA sequence specific for mycobacterium tuberculosis to be used for the diagnosis of tuberculosis |
| US6020124A (en) | 1992-04-27 | 2000-02-01 | Trustees Of Dartmouth College | Detection of soluble gene sequences in biological fluids |
| CA2167693C (en) | 1993-07-20 | 2007-01-30 | Ulrich Bogdahn | Melanoma-inhibiting protein |
| AU7375794A (en) | 1993-07-28 | 1995-02-28 | Akzo Nobel N.V. | Process for isolating nucleic acid from gram positive microorganisms |
| JP3696238B2 (ja) | 1993-08-30 | 2005-09-14 | プロメガ・コーポレイシヨン | 核酸精製用組成物及び方法 |
| US5631130A (en) | 1994-05-13 | 1997-05-20 | Abbott Laboratories | Materials and methods for the detection of Mycobacterium tuberculosis |
| ATE261496T1 (de) | 1994-06-09 | 2004-03-15 | Innogenetics Nv | Verfahren zum nachweis des antibiotikaresistenzspektrums von mykobakterium spezies |
| US5731150A (en) | 1995-11-01 | 1998-03-24 | Chiron Diagnostic Corporation | IS6110 based molecular detection of mycobacterium tuberculosis |
| US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| US6492144B1 (en) | 1997-05-30 | 2002-12-10 | Diagen Corporation | Methods for detection of nucleic acid sequences in urine |
| US20020119478A1 (en) | 1997-05-30 | 2002-08-29 | Diagen Corporation | Methods for detection of nucleic acid sequences in urine |
| PT2216416E (pt) | 1997-05-30 | 2012-08-16 | Trovagene Inc | Métodos para detecção de sequências de ácido nucleico na urina |
| US5962665A (en) | 1997-06-16 | 1999-10-05 | Abbott Laboratories | Nucleic acid primers and probes for detecting HIV-1 and HIV-2 |
| US20060204989A1 (en) * | 1998-09-22 | 2006-09-14 | Kopreski Michael S | Comparative analysis of extracellular RNA species |
| US7196183B2 (en) | 2001-08-31 | 2007-03-27 | Innogenetics N.V. | Hepatitis C virus genotype, and its use as prophylactic, therapeutic and diagnostic agent |
| US20040053264A1 (en) | 2002-02-01 | 2004-03-18 | Park Sung Sup | Clinical panel assay using DNA chips |
| EP2471924A1 (en) * | 2004-05-28 | 2012-07-04 | Asuragen, INC. | Methods and compositions involving microRNA |
| ITRM20050068A1 (it) | 2005-02-17 | 2006-08-18 | Istituto Naz Per Le Malattie I | Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine. |
| US7914982B2 (en) | 2005-02-17 | 2011-03-29 | Trovagene, Inc. | Methods for detecting pathogen specific nucleic acids in urine |
| ITRM20050067A1 (it) | 2005-02-17 | 2006-08-18 | Istituto Naz Per Le Malattie I | Metodo per la rivelazione di acidi nucleici virali o di origine virale nelle urine. |
| EP1885877A2 (en) | 2005-02-17 | 2008-02-13 | Istituto Nazionale per le Malattie Infettive "Lazzaro Spallanzani" IRCCS | Compositions and methods for detecting pathogen specific nucleic acids in urine |
| CA2597948A1 (en) | 2005-02-17 | 2006-08-24 | Istituto Nazionale Per Le Malattie Infettive Irccs Lazzaro Spallanzani | Compositions and methods for detecting viral specific nucleic acids in urine |
| US20060292616A1 (en) * | 2005-06-23 | 2006-12-28 | U.S. Genomics, Inc. | Single molecule miRNA-based disease diagnostic methods |
| DE102005059315A1 (de) * | 2005-12-09 | 2007-06-14 | Qiagen Gmbh | Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren |
| US20070202511A1 (en) * | 2006-02-28 | 2007-08-30 | Sigma-Aldrich Co. | Methods and compositions for the rapid isolation of small RNA molecules |
| ES2532153T3 (es) | 2008-07-18 | 2015-03-24 | Trovagene, Inc. | Métodos para la detección de secuencias de ácidos nucleicos "ultracortos" basados en PCR |
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2008
- 2008-08-22 WO PCT/US2008/009991 patent/WO2009025852A2/en not_active Ceased
- 2008-08-22 PL PL08795523T patent/PL2195450T3/pl unknown
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- 2008-08-22 AU AU2008289447A patent/AU2008289447B2/en not_active Ceased
- 2008-08-22 CN CN200880113585.0A patent/CN101861399B/zh not_active Expired - Fee Related
- 2008-08-22 ES ES13161985.0T patent/ES2660785T3/es active Active
- 2008-08-22 TR TR2018/02758T patent/TR201802758T4/tr unknown
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- 2008-08-22 EP EP13161985.0A patent/EP2612926B1/en not_active Not-in-force
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2017
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