HRP20220572T1 - Dobivanje radnih sojeva kvasca za metaboliziranje arabinoze - Google Patents
Dobivanje radnih sojeva kvasca za metaboliziranje arabinoze Download PDFInfo
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- HRP20220572T1 HRP20220572T1 HRP20220572TT HRP20220572T HRP20220572T1 HR P20220572 T1 HRP20220572 T1 HR P20220572T1 HR P20220572T T HRP20220572T T HR P20220572TT HR P20220572 T HRP20220572 T HR P20220572T HR P20220572 T1 HRP20220572 T1 HR P20220572T1
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- arabinose
- acetic acid
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- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 21
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims 20
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 19
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 15
- 238000000034 method Methods 0.000 claims 11
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 6
- 229910052799 carbon Inorganic materials 0.000 claims 6
- 238000000855 fermentation Methods 0.000 claims 6
- 230000004151 fermentation Effects 0.000 claims 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 5
- 239000008103 glucose Substances 0.000 claims 5
- 238000004519 manufacturing process Methods 0.000 claims 5
- 101150118141 GRE3 gene Proteins 0.000 claims 4
- 239000000463 material Substances 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 3
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 claims 3
- 102000016912 Aldehyde Reductase Human genes 0.000 claims 2
- 108010053754 Aldehyde reductase Proteins 0.000 claims 2
- 206010021143 Hypoxia Diseases 0.000 claims 2
- 241000235648 Pichia Species 0.000 claims 2
- 101150035354 araA gene Proteins 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 230000001146 hypoxic effect Effects 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- 241000194108 Bacillus licheniformis Species 0.000 claims 1
- 239000002028 Biomass Substances 0.000 claims 1
- 241000722885 Brettanomyces Species 0.000 claims 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 101150103567 HAA1 gene Proteins 0.000 claims 1
- 241001149669 Hanseniaspora Species 0.000 claims 1
- 229920002488 Hemicellulose Polymers 0.000 claims 1
- 241000235652 Pachysolen Species 0.000 claims 1
- OIRDTQYFTABQOQ-UHTZMRCNSA-N Vidarabine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@@H]1O OIRDTQYFTABQOQ-UHTZMRCNSA-N 0.000 claims 1
- 241000235013 Yarrowia Species 0.000 claims 1
- 240000008042 Zea mays Species 0.000 claims 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 1
- OIRDTQYFTABQOQ-UHFFFAOYSA-N ara-adenosine Natural products Nc1ncnc2n(cnc12)C1OC(CO)C(O)C1O OIRDTQYFTABQOQ-UHFFFAOYSA-N 0.000 claims 1
- 230000002759 chromosomal effect Effects 0.000 claims 1
- 235000005822 corn Nutrition 0.000 claims 1
- 239000000835 fiber Substances 0.000 claims 1
- MNQZXJOMYWMBOU-UHFFFAOYSA-N glyceraldehyde Chemical compound OCC(O)C=O MNQZXJOMYWMBOU-UHFFFAOYSA-N 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 230000010354 integration Effects 0.000 claims 1
- 230000000241 respiratory effect Effects 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
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Claims (12)
1. Postupak dobivanja i odabira sojeva kvasca koji mogu fermentirati arabinozu, glukozu i ksiloza u prisutnosti octene kiseline u nedisociranom obliku, naznačen time što se sastoji u:
– kromosomskoj integraciji gena araA, araB i araD, po mogućnosti araA iz vrste B. licheniformis, te araB i araD iz bakterije E. coli, kod soja kvasca koji može fermentirati ksilozu u prisutnosti octene kiseline u nedisociranom obliku i ima najmanje jedan inaktivirani ili izbačeni gen GRE3;
– stavljanju transformiranih sojeva u izravni uzgoj na mediju koji sadrži arabinozu kao jedini izvor ugljika radi odabira sojeva koji mogu metabolizirati navedenu arabinozu;
– mogućem odabiru transformiranih sojeva koji su metabolizirali arabinoza na njihovu aktivnost reduktaze aldoze manju od ili jednaku 0,002 jedinica po gramu protein, ili čak manju od ili jednaku 0,0005 jedinica po gramu proteina.
2. Postupak u skladu s patentnim zahtjevom 1, naznačen time što se soj kvasca koji može fermentirati ksilozu u prisutnosti octene kiseline u nedisociranom obliku i ima najmanje jedan inaktivirani ili izbačeni gen GRE3 bira između rodova Saccharomices, Schizosaccharomices, Pichia, Yarrowia, Paffia, Kluyveromices, Candida, Talaromices, Brettanomices, Pachysolen, Hansenula, Kloeckera, Schwanniomices i Debaryomices, po mogućnosti soj vrste Saccharomices cerevisiae.
3. Postupak u skladu s patentnim zahtjevom 2, naznačen time što je soj kvasca koji može fermentirati ksilozu u prisutnosti octene kiseline u nedisociranom obliku i ima najmanje jedan inaktivirani ili izbačeni gen GRE3 soj registriran kod CNCM 29. siječnja 2015. pod brojem 1-4953.
4. Soj kvasca dobiven postupkom u skladu s bilo kojim od patentnih zahtjeva 1 do 3, naznačen time što dodatno pokazuje aktivnost reduktaze aldoze manju od ili jednaku 0,002 jedinica po gramu proteina, ili čak manju od ili jednaku 0,0005 jedinica po gramu proteina, te je soj 1-5085, registriran kod CNCM (Collection Nationale de Cultures de Microorganismes, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15) 19. svibnja 2016.
5. Postupak dobivanja i odabira soja kvasca s poboljšanom sposobnosti fermentiranja arabinoze, glukoze i ksiloze u prisutnosti octene kiseline u nedisociranom obliku, gdje soj kvasca može fermentirati arabinozu, glukozu i ksiloza u prisutnosti octene kiseline u nedisociranom obliku i dobiva se postupkom u skladu s bilo kojim od patentnih zahtjeva 1 do 3, naznačen time što se po mogućnosti soj u skladu s patentnim zahtjevom 4 uzastopno uzgaja u sljedećim uvjetima:
– anaerobnog ili hipoksičnog uzgoja na mediju koji kao jedine izvore ugljika sadrži arabinozu i ksilozu u ograničavajućim koncentracijama radi proizvodnje biomase, po mogućnosti u količini od 5 g/l odnosno 4 g/l; zatim
– dvije uzastopne anaerobne ili hipoksične kulture u prisutnosti octene kiseline u nedisociranom obliku, jednu na mediju koji sadrži glukozu kao jedini izvor ugljika, a drugu na mediju koji sadrži ksilozu kao jedini izvor ugljika;
– mogućeg aerobnog uzgoja u minimalnom mediju koji kao jedini izvor ugljika sadrži izvor strogo respiratornog ugljika, po mogućnosti glicerol.
6. Postupak u skladu s patentnim zahtjevom 5, naznačen time što se uzastopni uzgoj soja u različitim medijima ponavlja najmanje 2 puta.
7. Soj kvasca kojeg se može dobiti postupkom u skladu s bilo kojim od patentnih zahtjeva 5 do 6, naznačen time što je soj 1-5086, registriran kod CNCM (Collection Nationale de Cultures de Microorganismes, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15) 19. svibnja 2016.
8. Soj kvasca u skladu s bilo kojim od patentnim zahtjevom 4 ili 7, naznačen time što ima najmanje jednu dodatnu kopiju gena HAA1, ubačenu po mogućnosti na razini gena GRE3.
9. Upotreba soja u skladu s bilo kojim od patentnih zahtjeva 4, 7 i 8 naznačen time što je namijenjen fermentaciji materijala, koji po mogućnosti sadrži arabinozu i/ili ksilozu, i/ili proizvodnji etanola.
10. Postupak proizvodnje produkata fermentacije ili etanola, naznačen time što se sastoji u sljedećim koracima:
– inkubacije materijala ili medija koji sadrži arabinozu i/ili ksilozu sa sojem u skladu s bilo kojim od patentnih zahtjeva 4, 7 i 8;
– fermentacije u anaerobnim ili poluanaerobnim uvjetima;
– prikupljanje jednog ili više produkata fermentacije, ili etanola.
11. Postupak proizvodnje produkata fermentacije ili etanola u skladu s patentnim zahtjevom 12, naznačen time što materijal ili medij također sadrži glukozu.
12. Postupak proizvodnje produkata fermentacije ili etanola u skladu s patentnim zahtjevom 10 ili 11, naznačen time što je materijal ili medij hemiceluloza ili "kukuruzna vlakna".
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1750550A FR3062134B1 (fr) | 2017-01-24 | 2017-01-24 | Obtention de souches de levure performantes pour la metabolisation de l'arabinose |
| PCT/EP2018/051708 WO2018138135A1 (fr) | 2017-01-24 | 2018-01-24 | Obtention de souches de levure performantes pour la metabolisation de l'arabinose |
| EP18712080.3A EP3574120B1 (fr) | 2017-01-24 | 2018-01-24 | Obtention de souches de levure performantes pour la metabolisation de l'arabinose |
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| Publication Number | Publication Date |
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| HRP20220572T1 true HRP20220572T1 (hr) | 2022-06-24 |
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| HRP20220572TT HRP20220572T1 (hr) | 2017-01-24 | 2018-01-24 | Dobivanje radnih sojeva kvasca za metaboliziranje arabinoze |
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| US (1) | US10920232B2 (hr) |
| EP (1) | EP3574120B1 (hr) |
| JP (1) | JP7181876B2 (hr) |
| CN (1) | CN110199026A (hr) |
| AR (1) | AR110848A1 (hr) |
| AU (1) | AU2018213056B2 (hr) |
| BR (1) | BR112019014115A2 (hr) |
| CA (1) | CA3049415A1 (hr) |
| DK (1) | DK3574120T3 (hr) |
| ES (1) | ES2912995T3 (hr) |
| FR (1) | FR3062134B1 (hr) |
| HR (1) | HRP20220572T1 (hr) |
| HU (1) | HUE058951T2 (hr) |
| MY (1) | MY193450A (hr) |
| PL (1) | PL3574120T3 (hr) |
| PT (1) | PT3574120T (hr) |
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| CN110872596B (zh) * | 2018-09-04 | 2021-08-24 | 中国科学院分子植物科学卓越创新中心 | 木糖阿拉伯糖共利用的酿酒酵母菌的构建方法 |
| CN113215203B (zh) * | 2021-02-26 | 2023-06-09 | 中粮生化能源（肇东）有限公司 | 共发酵酵母菌扩培发酵产乙醇的方法 |
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| SE0202090D0 (sv) | 2002-05-08 | 2002-07-04 | Forskarpatent I Syd Ab | A modifierd yeast consuming L-arabinose |
| CA2664646C (en) * | 2006-10-02 | 2016-09-20 | Dsm Ip Assets B.V. | Metabolic engineering of arabinose- fermenting yeast cells |
| DE102007016534B4 (de) | 2007-04-05 | 2019-03-28 | Butalco Gmbh | Neuer Expressionsvektor von Codon-optimierten Genen eines Arabinose-Stoffwechselweges zur Arabinose-Umsetzung in Hefe |
| JP5219074B2 (ja) * | 2008-01-24 | 2013-06-26 | 独立行政法人産業技術総合研究所 | キシロース発酵能が優れた六炭糖・五炭糖同時発酵酵母およびそれを用いたエタノールの高効率生産方法 |
| US20110104736A1 (en) * | 2008-03-13 | 2011-05-05 | Jacobus Thomas Pronk | Selection of organisms capable of fermenting mixed substrates |
| DE102008031350B4 (de) | 2008-07-02 | 2011-02-10 | Johann Wolfgang Goethe-Universität Frankfurt am Main | Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen |
| UA108853C2 (uk) * | 2009-07-10 | 2015-06-25 | Спосіб ферментації галактози | |
| FR2953857B1 (fr) | 2009-12-15 | 2012-10-12 | Lesaffre & Cie | Nouvelles souches de levure pour la production d'alcool |
| CN102869766B (zh) * | 2010-04-21 | 2015-11-25 | 帝斯曼知识产权资产管理有限公司 | 适于发酵混合的糖组合物的细胞 |
| KR101246780B1 (ko) * | 2010-10-06 | 2013-03-26 | 한국과학기술원 | 아라비노스 대사경로가 도입된 자일리톨 생산균주 및 이를 이용한 자일리톨 생산방법 |
| FR2968313B1 (fr) | 2010-12-03 | 2014-10-10 | Lesaffre & Cie | Procede de preparation d'une levure industrielle, levure industrielle et application a la production d'ethanol a partir d'au moins un pentose |
| MX2013012269A (es) * | 2011-04-22 | 2013-11-22 | Dsm Ip Assets Bv | Celula de levadura capaz de convertir azucares que incluyen arabinosa y xilosa. |
| AR087423A1 (es) * | 2011-08-04 | 2014-03-19 | Dsm Ip Assets Bv | Celula capaz de fermentar azucares pentosas |
| FR2991340B1 (fr) | 2012-06-01 | 2016-02-19 | Lesaffre & Cie | Procede d'obtention de souches de levure ameliorees par modification genetique et croisement |
| FR2991339B1 (fr) * | 2012-06-01 | 2016-02-19 | Lesaffre & Cie | Souches de levure aptes a metaboliser le xylose et resistantes a au moins un inhibiteur de fermentation, procede d'obtention et utilisation |
| JP6236634B2 (ja) * | 2012-08-24 | 2017-11-29 | 国立大学法人神戸大学 | バイオマスからのエタノールの生産方法 |
| WO2014207087A1 (en) * | 2013-06-26 | 2014-12-31 | Abengoa Bioenergia Nuevas Tecnologias S.A. | Production of advanced fuels and of chemicals by yeasts on the basis of second generation feedstocks |
| FR3017623B1 (fr) | 2014-02-17 | 2018-08-10 | Lesaffre Et Compagnie | Souche fermentant les pentoses a propagation optimisee |
| FR3035405B1 (fr) * | 2015-04-27 | 2019-04-19 | Lesaffre Et Compagnie | Souche de levure presentant une capacite amelioree a fermenter le xylose en presence d'acide acetique |
-
2017
- 2017-01-24 FR FR1750550A patent/FR3062134B1/fr active Active
-
2018
- 2018-01-24 CN CN201880006369.XA patent/CN110199026A/zh active Pending
- 2018-01-24 AR ARP180100162A patent/AR110848A1/es unknown
- 2018-01-24 DK DK18712080.3T patent/DK3574120T3/da active
- 2018-01-24 PT PT187120803T patent/PT3574120T/pt unknown
- 2018-01-24 CA CA3049415A patent/CA3049415A1/en active Pending
- 2018-01-24 AU AU2018213056A patent/AU2018213056B2/en active Active
- 2018-01-24 ES ES18712080T patent/ES2912995T3/es active Active
- 2018-01-24 WO PCT/EP2018/051708 patent/WO2018138135A1/fr unknown
- 2018-01-24 JP JP2019537238A patent/JP7181876B2/ja active Active
- 2018-01-24 PL PL18712080T patent/PL3574120T3/pl unknown
- 2018-01-24 BR BR112019014115-4A patent/BR112019014115A2/pt unknown
- 2018-01-24 EP EP18712080.3A patent/EP3574120B1/fr active Active
- 2018-01-24 HR HRP20220572TT patent/HRP20220572T1/hr unknown
- 2018-01-24 US US16/475,845 patent/US10920232B2/en active Active
- 2018-01-24 HU HUE18712080A patent/HUE058951T2/hu unknown
- 2018-01-24 MY MYPI2019003706A patent/MY193450A/en unknown
-
2019
- 2019-06-28 ZA ZA2019/04267A patent/ZA201904267B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2912995T3 (es) | 2022-05-30 |
| EP3574120B1 (fr) | 2022-04-06 |
| WO2018138135A1 (fr) | 2018-08-02 |
| HUE058951T2 (hu) | 2022-09-28 |
| AU2018213056A1 (en) | 2019-07-25 |
| AU2018213056B2 (en) | 2023-10-19 |
| US20190330645A1 (en) | 2019-10-31 |
| AR110848A1 (es) | 2019-05-08 |
| JP2020506678A (ja) | 2020-03-05 |
| US10920232B2 (en) | 2021-02-16 |
| EP3574120A1 (fr) | 2019-12-04 |
| MY193450A (en) | 2022-10-13 |
| PT3574120T (pt) | 2022-05-13 |
| CA3049415A1 (en) | 2018-08-02 |
| DK3574120T3 (da) | 2022-05-23 |
| BR112019014115A2 (pt) | 2020-02-11 |
| FR3062134B1 (fr) | 2023-07-21 |
| ZA201904267B (en) | 2022-03-30 |
| FR3062134A1 (fr) | 2018-07-27 |
| PL3574120T3 (pl) | 2022-07-04 |
| CN110199026A (zh) | 2019-09-03 |
| JP7181876B2 (ja) | 2022-12-01 |
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