JP5059100B2 - 水素製造方法及び装置並びにそれに用いる微生物固定化担体 - Google Patents
水素製造方法及び装置並びにそれに用いる微生物固定化担体 Download PDFInfo
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Description
・グラム染色:陽性
・形態 :桿菌
・酸素要求性:嫌気性(偏性)
であり、分類学上の位置は、
属:Thermoanaerobacterium
種:thermosaccharolyticum
である。
12…水素発酵槽
14…pH制御装置
16…供給管
18…担体
20…ガス管
22…廃液管
24…調整剤供給管
26…開閉弁
28…pH測定計
ウレタンアクリレートプレポリマー 15%
滅菌水 64.25%
NNN’N’テトラメチルエチレンジアミン 0.5%
過硫酸カリウム 0.25%
このゲルを3mm角に成形し、包括固定化担体を得た。得られた担体を500mLジャーファーメンターに10%投入し、表1に示す培地を用いて各種pHでの連続発酵を行った。図2はその結果である。なお、運転条件は、55℃、培地投入速度100〜300mL/日である。
実施例1として、Thermoanaerobacterium PEH8株が固定化されている包括固定化水素発酵担体を用い、図3の水素製造装置10で水素発酵を行った。担体は以下のように作製した。すなわち、Thermoanaerobacterium PEH8株をウレタンアクリレートプレポリマー液に懸濁し、過硫酸カリウムとNNN’N’テトラメチルエチレンジアミンを添加することにより重合し、菌を包括したゲルを得た。それぞれの添加量は次のとおりである。
Thermoanaerobacterium PEH8株 7×107cells/ml溶液 20%
ウレタンアクリレートプレポリマー 15%
滅菌水 64.25%
NNN’N’テトラメチルエチレンジアミン 0.5%
過硫酸カリウム 0.25%
このゲルを3mm角に成形し、包括固定化担体を得た。得られた担体を1Lジャーファーメンターに10%投入し、表1で示した培地を滞留時間48時間で投入し、水素発酵槽pH5で水素発酵試験を行った。
第2試験では、バイオマスを原料として水素発酵の試験を行った。
[原料調製法]
バガス(サトウキビ搾り粕)等リグノセルロース系資源の可溶化及び糖化を行う方法としては、濃硫酸や希硫酸等の酸を用いる方法、水酸化ナトリウムやアンモニウム等のアルカリを用いる方法、超臨界水を用いる方法、酵素を用いる方法などがある。先ず、バガス1.6kg及び2%(w/w)水酸化ナトリウム水溶液16kgを30L培養槽(HMF−30F01、日立製作所(株)製)に投入し、121℃、15分の条件で熱処理を行った。冷却後80メッシュの篩にて固液分離を行い、篩上の固形分4.5kgに水18kgを添加し、ホモジナイズ処理を行った。硫酸にてpHを5.0に調整した後、市販の酵素(GC220;32g、Multifect Xylanase;32g、Genencor International)を添加し、55℃、72時間の条件で酵素処理を行った。その後、酵母エキス(PRONAL NS 55 T4、PRODESA)230gを添加し、これを原料液とした。原料液の組成は、フェノール硫酸法による全糖値が9500mg/L、HPLCによる還元糖分析値が、グルコース5973mg/L、キシロース1776mg/L、アラビノース31mg/Lであった。
[微生物包括固定化担体作製法]
実施例1に同じ。
[水素発酵]
得られた担体を1Lジャーファーメンター(エイブル(株)製)に10%投入し、上記原料を滞留時間72時間で投入し、水素発酵槽pH5.0で水素発酵を行った。バイオガスの発生が安定した培養開始後10日目から19日目までの連続運転の結果を図5に示す。なお、図5横軸の培養日数(日)1、2〜10はそれぞれ培養開始後10日目、11日目〜19日目を示している。また、図5中の折れ線グラフは、水素の体積比率を示すと共に、図5中の棒グラフは、発酵ガス生産量(ml/日)を示している。
Claims (4)
- 微生物を利用して有機物から水素を生成させる水素製造方法において、
耐酸性を有する水素生成菌を包括固定化した担体を用い、該担体をpH4〜6の環境下で前記有機物と接触反応させることによって水素を生成させると共に、
前記水素生成菌として、独立行政法人産業技術総合研究所特許生物寄託センターにThermoanaerobacterium PEH8株として寄託されている国際寄託の受託番号FERM BP-10804菌株を用いることを特徴とする水素製造方法。 - 微生物を利用して有機物から水素を生成させる水素製造装置において、
耐酸性を有する水素生成菌であって、独立行政法人産業技術総合研究所特許生物寄託センターにThermoanaerobacterium PEH8株として寄託されている国際寄託の受託番号FERM BP-10804菌株を用いた水素生成菌が包括固定化された担体が投入されるとともに、該担体が前記有機物と接触反応することによって水素を生成する水素発酵槽と、
前記水素発酵槽のpHを4〜6の範囲に制御するpH制御装置と、を備えたことを特徴とする水素製造装置。 - 前記水素発酵槽の後段には、該水素発酵槽から流出する廃液をメタン発酵させるメタン発酵槽が設けられることを特徴とする請求項2に記載の水素製造装置。
- 耐酸性を有する水素生成菌であって、独立行政法人産業技術総合研究所特許生物寄託センターにThermoanaerobacterium PEH8株として寄託されている国際寄託の受託番号FERM BP-10804菌株を用いた水素生成菌が包括固定化されたことを特徴とする微生物固定化担体。
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JP2009507479A JP5059100B2 (ja) | 2007-03-29 | 2008-03-26 | 水素製造方法及び装置並びにそれに用いる微生物固定化担体 |
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PCT/JP2008/055689 WO2008120629A1 (ja) | 2007-03-29 | 2008-03-26 | 水素製造方法及び装置並びにそれに用いる微生物固定化担体 |
JP2009507479A JP5059100B2 (ja) | 2007-03-29 | 2008-03-26 | 水素製造方法及び装置並びにそれに用いる微生物固定化担体 |
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US (1) | US8227222B2 (ja) |
JP (1) | JP5059100B2 (ja) |
CN (1) | CN101668862B (ja) |
BR (1) | BRPI0809362A2 (ja) |
WO (1) | WO2008120629A1 (ja) |
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US9528656B1 (en) | 2013-10-14 | 2016-12-27 | Arrowhead Center, Inc. | Elastomeric, hydrogen-resistant biopolymer and its use in oil and gas refining, and in the storage and transport of hydrogen gas |
CN105219667B (zh) * | 2014-06-30 | 2018-09-21 | 北京化工大学 | 用于木糖发酵制氢的菌株及制氢方法 |
CN106959714B (zh) * | 2017-03-17 | 2019-05-14 | 贵州省煤炭产品质量监督检验院 | 一种智能清洁能源的微生物反应控制系统 |
EP3676364A1 (de) * | 2017-08-30 | 2020-07-08 | Helmholtz-Zentrum für Umweltforschung GmbH - UFZ | Verfahren zur einstellung des gehalts von flüchtigen organischen säuren (fos) in einem mit organischem substrat beschickten biogasreaktor sowie mit mikroorganismen beladene pellets |
EP4379059A1 (en) * | 2022-12-01 | 2024-06-05 | Université d'Aix-Marseille | New thermophilic bacteria strains to produce hydrogen in acidic conditions |
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JPH0731998A (ja) | 1993-07-13 | 1995-02-03 | Ebara Res Co Ltd | 有機性廃棄物の微嫌気水素発酵法 |
JP2001023677A (ja) | 1999-07-13 | 2001-01-26 | Ebara Corp | 燃料電池発電方法及び燃料電池発電システム |
JP2001229955A (ja) | 2000-02-14 | 2001-08-24 | Toto Ltd | 発電システム |
JP3851806B2 (ja) | 2001-11-06 | 2006-11-29 | 株式会社タクマ | 微生物を用いた水素製造方法及び装置 |
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- 2008-03-26 WO PCT/JP2008/055689 patent/WO2008120629A1/ja active Application Filing
- 2008-03-26 BR BRPI0809362-8A patent/BRPI0809362A2/pt not_active IP Right Cessation
- 2008-03-26 CN CN2008800108663A patent/CN101668862B/zh not_active Expired - Fee Related
- 2008-03-26 JP JP2009507479A patent/JP5059100B2/ja not_active Expired - Fee Related
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CN101668862B (zh) | 2013-06-26 |
CN101668862A (zh) | 2010-03-10 |
US8227222B2 (en) | 2012-07-24 |
BRPI0809362A2 (pt) | 2014-09-02 |
WO2008120629A1 (ja) | 2008-10-09 |
JPWO2008120629A1 (ja) | 2010-07-15 |
US20100136643A1 (en) | 2010-06-03 |
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