HRP20220205T1 - Transgena mišja životinja koja proizvodi antitijela - Google Patents

Transgena mišja životinja koja proizvodi antitijela Download PDF

Info

Publication number
HRP20220205T1
HRP20220205T1 HRP20220205TT HRP20220205T HRP20220205T1 HR P20220205 T1 HRP20220205 T1 HR P20220205T1 HR P20220205T T HRP20220205T T HR P20220205TT HR P20220205 T HRP20220205 T HR P20220205T HR P20220205 T1 HRP20220205 T1 HR P20220205T1
Authority
HR
Croatia
Prior art keywords
host according
light chain
transgenic host
transgenic
nucleic acid
Prior art date
Application number
HRP20220205TT
Other languages
English (en)
Inventor
Erwin Houtzager
Rui Daniel Pinto
Ton Logtenberg
Mark Throsby
Original Assignee
Merus N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41280336&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HRP20220205(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Merus N.V. filed Critical Merus N.V.
Publication of HRP20220205T1 publication Critical patent/HRP20220205T1/hr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Claims (19)

1. Transgeni mišji domaćin koji je u stanju da proizvodi imunoglobuline koji sadrže ljudsku VL regiju, pri čemu navedeni mišji domaćin sadrži integriranu u svojoj germinativnoj liniji preuređenu nukleinsku kiselinu koja sadrži jedan ljudske germinativne linije V i jedan ljudske germinativne linije J genski segment koji kodira preuređenu ljudsku VL regiju i konstantnu regiju lakog lanca bilo koje životinjske vrste, pri čemu je navedena varijabilna regija lakog lanca u stanju da se upari sa najmanje dva različita teška lanca kodirana od strane domaćina, tako da se raznolikost u specifičnosti antitijela koja sadrže navedenu ljudsku VL regiju zadržava kroz preuređivanja i hipermutacije u teškim lancima.
2. Transgeni domaćin prema patentnom zahtjevu 1, pri čemu je domaćin miš.
3. Transgeni domaćin prema patentnom zahtjevu 1 ili 2, pri čemu navedena konstantna regija lakog lanca je mišja konstantna regija lakog lanca.
4. Transgeni domaćin prema bilo kojem od patentnih zahtjeva 1-3, pri čemu je integracija u lokusu koji je otporan na utišavanje.
5. Transgeni domaćin prema bilo kojem od patentnih zahtjeva 1-3, pri čemu je integracija u Rosa-lokusu.
6. Transgeni domaćin prema bilo kojem od patentnih zahtjeva 1-5, pri čemu je varijabilna regija lakog lanca koja kodira nukleinsku kiselinu osigurana sredstvom koje omogućava eksprimiranje spomenute nukleinske kiseline suštinski ograničeno na stanice B stanične loze.
7. Transgeni domaćin prema patentnom zahtjevu 6, pri čemu je varijabilna regija lakog lanca koja kodira nukleinsku kiselinu osigurana sredstvom koje omogućava eksprimiranje varijabilne regije lakog lanca koja kodira nukleinsku kiselinu pretežno tokom određene faze razvoja B stanica.
8. Transgeni domaćin prema patentnom zahtjevu 7, pri čemu navedeno sredstvo sadrži promoter odabran iz grupe CD19, CD20, µHC, VpreB1, VpreB2, VpreB3, λ5, Igα, Igβ, κLC, λLC, i BSAP (Pax5).
9. Transgeni domaćin prema patentnom zahtjevu 7 ili 8, pri čemu navedeno sredstvo sadrži cre-lox sustav.
10. Transgeni domaćin prema bilo kojem od patentnih zahtjeva 1-9, pri čemu je navedena varijabilna regija lakog lanca sposobna za uparivanje sa najmanje dva teška lanca domaćina.
11. Transgeni domaćin prema bilo kojem od patentnih zahtjeva 1-9, pri čemu je navedena varijabilna regija lakog lanca sposobna za uparivanje sa najmanje dva ljudska teška lanca.
12. Transgeni domaćin prema bilo kojem od patentnih zahtjeva 1-11, pri čemu je najmanje jedan od endogenih lokusa koji kodira endogeni laki lanac funkcionalno utišan.
13. Transgeni domaćin prema bilo kojem od patentnih zahtjeva 1-12, pri čemu je lokus endogenog κ lakog lanca funkcionalno utišan.
14. Transgeni domaćin prema bilo kojem od patentnih zahtjeva 1-13, pri čemu sekvenca varijabilne regije lakog lanca koja kodira nukleinsku kiselinu je sekvenca vκ ljudske germinativne linije.
15. Transgeni domaćin prema patentnom zahtjevu 14, pri čemu sekvenca vκ germinativne linije je na bazi 012.
16. Transgeni domaćin prema patentnom zahtjevu 15, pri čemu sekvenca vκ germinativne linije je IGKV1-39*01 / IGKJ1*01.
17. Postupak za proizvodnju željenog antitijela koji obuhvaća izlaganje domaćina prema bilo kojem od patentnih zahtjeva 1-16 antigenu tako da je odgovor antitijela induciran i izoliranje antitijela specifičnih za antigen.
18. Postupak za proizvodnju željenog antitijela koji obuhvaća izlaganje domaćina prema bilo kojem od patentnih zahtjeva 1-16 antigenu tako da je odgovor antitijela induciran i izoliranje stanica koje proizvode takva antitijela, kultivaciju i prikupljanje spomenutih antitijela.
19. Postupak za proizvodnju željenog antitijela koji obuhvaća izlaganje domaćina prema bilo kojem od patentnih zahtjeva 1-16 antigenu tako da je odgovor antitijela induciran i izoliranje nukleinske kiseline koja kodira najmanje dio takvog antitijela, umetanje navedene nukleinske kiseline ili kopije ili derivata iste u ekspresijsku kasetu i eksprimiranje navedenog antitijela u stanici domaćina.
HRP20220205TT 2008-06-27 2009-06-29 Transgena mišja životinja koja proizvodi antitijela HRP20220205T1 (hr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL2008050430 2008-06-27
EP18183980.4A EP3456190B1 (en) 2008-06-27 2009-06-29 Antibody producing transgenic murine animal

Publications (1)

Publication Number Publication Date
HRP20220205T1 true HRP20220205T1 (hr) 2022-05-27

Family

ID=41280336

Family Applications (2)

Application Number Title Priority Date Filing Date
HRP20220205TT HRP20220205T1 (hr) 2008-06-27 2009-06-29 Transgena mišja životinja koja proizvodi antitijela
HRP20140139AT HRP20140139T1 (hr) 2008-06-27 2014-02-13 Protutijela koja proizvode nehumani sisavci

Family Applications After (1)

Application Number Title Priority Date Filing Date
HRP20140139AT HRP20140139T1 (hr) 2008-06-27 2014-02-13 Protutijela koja proizvode nehumani sisavci

Country Status (23)

Country Link
EP (6) EP3456191A1 (hr)
JP (6) JP5749161B2 (hr)
KR (5) KR20110029156A (hr)
CN (3) CN102123582B (hr)
AU (1) AU2009263082C1 (hr)
CA (1) CA2729095C (hr)
CY (2) CY1114830T1 (hr)
DK (3) DK2147594T3 (hr)
ES (3) ES2906344T3 (hr)
HK (1) HK1154463A1 (hr)
HR (2) HRP20220205T1 (hr)
HU (1) HUE057994T2 (hr)
LT (1) LT3456190T (hr)
MX (1) MX2010014542A (hr)
NZ (1) NZ590118A (hr)
PL (2) PL2147594T3 (hr)
PT (3) PT2147594E (hr)
RS (2) RS53146B (hr)
RU (2) RU2731084C2 (hr)
SG (3) SG10201408500RA (hr)
SI (2) SI2147594T1 (hr)
WO (1) WO2009157771A2 (hr)
ZA (1) ZA201009312B (hr)

Families Citing this family (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2965865C (en) 2002-07-18 2021-10-19 Merus N.V. Recombinant production of mixtures of antibodies
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
EP2395016A3 (en) 2003-05-30 2012-12-19 Merus B.V. Design and use of paired variable regions of specific binding molecules
GB2404382B (en) 2003-07-28 2008-01-30 Oxitec Ltd Pest control
GB2443186A (en) 2006-10-25 2008-04-30 Oxitec Ltd Expression system for mediating alternative splicing
ES2906344T3 (es) * 2008-06-27 2022-04-18 Merus Nv Animal murino transgénico productor de anticuerpos
LT2346994T (lt) 2008-09-30 2022-03-10 Ablexis, Llc Knock-in pelė, skirta chimerinių antikūnų gamybai
CN102638971B (zh) 2009-07-08 2015-10-07 科马布有限公司 动物模型及治疗分子
US9445581B2 (en) 2012-03-28 2016-09-20 Kymab Limited Animal models and therapeutic molecules
US20130045492A1 (en) 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
US9796788B2 (en) 2010-02-08 2017-10-24 Regeneron Pharmaceuticals, Inc. Mice expressing a limited immunoglobulin light chain repertoire
RU2724663C2 (ru) * 2010-02-08 2020-06-25 Ридженерон Фармасьютикалз, Инк. Мышь с общей легкой цепью
US20120021409A1 (en) 2010-02-08 2012-01-26 Regeneron Pharmaceuticals, Inc. Common Light Chain Mouse
AU2013204019B2 (en) * 2010-02-08 2014-02-27 Regeneron Pharmaceuticals, Inc. Common light chain mouse
US9580491B2 (en) 2010-03-31 2017-02-28 Ablexis, Llc Genetic engineering of non-human animals for the production of chimeric antibodies
CN103228130B (zh) * 2010-06-17 2016-03-16 科马布有限公司 动物模型及治疗分子
CA2806233C (en) 2010-07-26 2021-12-07 Trianni, Inc. Transgenic animals and methods of use
US10662256B2 (en) 2010-07-26 2020-05-26 Trianni, Inc. Transgenic mammals and methods of use thereof
US10793829B2 (en) 2010-07-26 2020-10-06 Trianni, Inc. Transgenic mammals and methods of use thereof
NZ606824A (en) * 2010-08-02 2015-05-29 Regeneron Pharma Mice that make binding proteins comprising vl domains
MY172718A (en) * 2011-08-05 2019-12-11 Regeneron Pharma Humanized universal light chain mice
JP2014533930A (ja) * 2011-09-19 2014-12-18 カイマブ・リミテッド 免疫グロブリン遺伝子多様性の操作およびマルチ抗体治療薬
CA2791109C (en) 2011-09-26 2021-02-16 Merus B.V. Generation of binding molecules
EP2761008A1 (en) 2011-09-26 2014-08-06 Kymab Limited Chimaeric surrogate light chains (slc) comprising human vpreb
US9253965B2 (en) 2012-03-28 2016-02-09 Kymab Limited Animal models and therapeutic molecules
EP3816284A1 (en) 2011-12-22 2021-05-05 F. Hoffmann-La Roche AG Expression vector for antibody production in eukaryotic cells
PL2809150T3 (pl) * 2012-02-01 2020-04-30 Regeneron Pharmaceuticals, Inc. Humanizowane myszy wykazujące ekspresję łańcuchów ciężkich zawierających domeny VL
GB2500113A (en) 2012-03-05 2013-09-11 Oxitec Ltd Arthropod male germline gene expression system
EP3165086A1 (en) * 2012-03-06 2017-05-10 Regeneron Pharmaceuticals, Inc. Common light chain mouse
PT2825037T (pt) 2012-03-16 2019-08-07 Regeneron Pharma Animais não humanos que expressam sequências de imunoglobulinas sensíveis ao ph
SG10201607727PA (en) 2012-03-16 2016-11-29 Regeneron Pharma Mice that produce antigen-binding proteins with ph-dependent binding characteristics
US20140013456A1 (en) 2012-03-16 2014-01-09 Regeneron Pharmaceuticals, Inc. Histidine Engineered Light Chain Antibodies and Genetically Modified Non-Human Animals for Generating the Same
HUE053310T2 (hu) 2012-03-16 2021-06-28 Regeneron Pharma Hisztidinmódosított könnyûlánc antitestek és genetikailag módosított rágcsálók ugyanennek az elõállítására
US10251377B2 (en) 2012-03-28 2019-04-09 Kymab Limited Transgenic non-human vertebrate for the expression of class-switched, fully human, antibodies
GB2502127A (en) * 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
AU2013249985B2 (en) 2012-04-20 2017-11-23 Merus N.V. Methods and means for the production of Ig-like molecules
JP2015525071A (ja) * 2012-06-05 2015-09-03 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 共通の軽鎖を用いて完全ヒト型二重特異性抗体を作製するための方法
SG11201408646VA (en) 2012-07-06 2015-01-29 Genmab Bv Dimeric protein with triple mutations
CN103571872B (zh) * 2012-08-09 2016-11-23 山东国际生物科技园发展有限公司 一种能够表达人抗体的转基因动物的制备方法
ES2692951T3 (es) 2012-09-27 2018-12-05 Merus N.V. Anticuerpos IgG biespecíficos como acopladores de células T
GB201303932D0 (en) 2013-03-05 2013-04-17 Oxitec Ltd Muscle actin promoter
RU2689664C2 (ru) * 2013-03-13 2019-05-28 Регенерон Фарматютикалз, Инк. Мыши, экспрессирующие ограниченный репертуар легких цепей иммуноглобулина
MX368258B (es) 2013-03-15 2019-09-25 Zymeworks Inc Compuestos citotoxicos y antimitoticos y metodos de uso de los mismos.
US9788534B2 (en) 2013-03-18 2017-10-17 Kymab Limited Animal models and therapeutic molecules
US9783618B2 (en) 2013-05-01 2017-10-10 Kymab Limited Manipulation of immunoglobulin gene diversity and multi-antibody therapeutics
US9783593B2 (en) 2013-05-02 2017-10-10 Kymab Limited Antibodies, variable domains and chains tailored for human use
US11707056B2 (en) 2013-05-02 2023-07-25 Kymab Limited Animals, repertoires and methods
SG10201802295XA (en) 2013-10-01 2018-04-27 Kymab Ltd Animal Models and Therapeutic Molecules
CA2926090C (en) * 2013-10-15 2022-07-05 Regeneron Pharmaceuticals, Inc. Humanized il-15 animals
CN106255513B (zh) 2013-12-27 2022-01-14 酵活有限公司 用于药物偶联物的含磺酰胺连接系统
KR20240042540A (ko) 2014-02-28 2024-04-02 메뤼스 엔.페. ErbB-2와 ErbB-3에 결합하는 항체
JP6771385B2 (ja) 2014-02-28 2020-10-21 メルス ナムローゼ フェンノートシャップ 二重特異性抗体および医薬組成物
KR102601491B1 (ko) 2014-03-21 2023-11-13 리제너론 파마슈티칼스 인코포레이티드 단일 도메인 결합 단백질을 생산하는 비-인간 동물
AU2015231025A1 (en) 2014-03-21 2016-09-15 Regeneron Pharmaceuticals, Inc. Vl antigen binding proteins exhibiting distinct binding characteristics
GB2526867A (en) * 2014-06-05 2015-12-09 Oxitec Ltd Gene expression system
IL287645B2 (en) 2014-09-17 2024-04-01 Zymeworks Bc Inc Cytotoxic and anti-mitotic compounds and methods for their use
CN107438622A (zh) 2015-03-19 2017-12-05 瑞泽恩制药公司 选择结合抗原的轻链可变区的非人动物
CA2991880A1 (en) 2015-07-10 2017-01-19 Merus N.V. Human cd3 binding antibody
WO2017035241A1 (en) 2015-08-24 2017-03-02 Trianni, Inc. Enhanced production of immunoglobulins
SI3365373T1 (sl) 2015-10-23 2021-08-31 Merus N.V. Vezne molekule, ki zaviranjo rast raka
US10813346B2 (en) 2015-12-03 2020-10-27 Trianni, Inc. Enhanced immunoglobulin diversity
DK3411476T3 (da) 2016-02-04 2024-07-15 Trianni Inc Forøget produktion af immunoglobuliner
CA3026088A1 (en) 2016-06-03 2017-12-07 Regeneron Pharmaceuticals, Inc. Non-human animals expressing exogenous terminal deoxynucleotidyltransferase
US11827876B2 (en) 2016-08-12 2023-11-28 Oxitec Ltd. Self-limiting, sex-specific gene and methods of using
CN109983033B (zh) 2016-09-23 2023-10-31 美勒斯公司 调节细胞表达的生物活性的结合分子
WO2018175390A1 (en) * 2017-03-20 2018-09-27 Washington University Cells and methods of uses and making the same
MX2019011660A (es) 2017-03-31 2019-11-18 Merus Nv Anticuerpos biespecificos que se unen al receptor 2 del factor de crecimiento humano (erbb-2) y receptor 3 del factor de crecimiento humano (erbb3) para usarse en el tratamiento de celulas que tienen un gen de fusion de neuregulina-1 (nrg1).
BR112019020507A2 (pt) 2017-03-31 2020-08-04 Merus N.V. agente de alvejamento de erbb-2 e um anticorpo bispecífico com locais de ligação de antígenos que ligam um epítopo em uma parte extracelular de erbb-2 e erbb-3 para tratamento de um indivíduo com um tumor positivo erbb-2, erbb-2 / erbb-3
CN111148764A (zh) 2017-05-17 2020-05-12 美勒斯公司 用于乳腺癌的ErbB-2/ErbB-3双特异性抗体与内分泌治疗的组合
IL271833B2 (en) 2017-07-06 2024-09-01 Merus Nv Antibodies that regulate biological activity expressed by a cell
WO2019009727A1 (en) 2017-07-06 2019-01-10 Merus N.V. ANTI-PD1 ANTI-TIM3 BISPECIFIC ANTIBODIES
EP3649154A1 (en) 2017-07-06 2020-05-13 Merus N.V. Binding molecules that modulate a biological activity expressed by a cell
GB201710984D0 (en) * 2017-07-07 2017-08-23 Kymab Ltd Cells, vertebrates, populations & methods
KR20200042485A (ko) 2017-08-09 2020-04-23 메뤼스 엔.페. EGFR 및 cMET에 결합하는 항체
EP3717008A1 (en) 2017-12-01 2020-10-07 Merus N.V. Use of bispecific antibody and il-15 for combination therapy
JP7430636B2 (ja) * 2017-12-05 2024-02-13 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 操作された免疫グロブリンラムダ軽鎖を有する非ヒト動物及びその使用
TWI849895B (zh) 2018-02-09 2024-07-21 日商小野藥品工業股份有限公司 雙特異性抗體
WO2019190922A1 (en) 2018-03-24 2019-10-03 Regeneron Pharmaceuticals, Inc. Genetically modified non-human animals for generating therapeutic antibodies against peptide-mhc complexes, methods of making and uses thereof
AR115320A1 (es) 2018-03-30 2020-12-23 Merus Nv Anticuerpo multivalente
CN108624622A (zh) * 2018-05-16 2018-10-09 湖南艾佳生物科技股份有限公司 一种基于CRISPR-Cas9系统构建的能分泌小鼠白细胞介素-6的基因工程细胞株
KR102480493B1 (ko) * 2018-06-08 2022-12-21 크리스탈 바이오사이언스 주식회사 동일한 경쇄 i을 갖는 다양한 항체를 생산하기 위한 트랜스제닉 동물
IL305557A (en) 2018-06-14 2023-10-01 Regeneron Pharma Non-human animals with transgenic DH–DH rearrangement capacity, and their uses
CN113366018A (zh) 2018-12-20 2021-09-07 美勒斯公司 用于治疗疾病的CLEC12AxCD3双特异性抗体和方法
US20220090125A1 (en) 2018-12-21 2022-03-24 Compass Therapeutics Llc Transgenic mouse expressing common human light chain
CA3124768A1 (en) * 2018-12-31 2020-07-09 Merus N.V. Mixed binding domains
CN113439089A (zh) 2018-12-31 2021-09-24 美勒斯公司 截短的多价多元体
WO2020154293A1 (en) 2019-01-22 2020-07-30 Bristol-Myers Squibb Company Antibodies against il-7r alpha subunit and uses thereof
KR20210126703A (ko) 2019-02-14 2021-10-20 메뤼스 엔.페. Egfr, her2 및 her3에 결합하는 결합 모이어티들의 병용물
WO2020167122A1 (en) 2019-02-14 2020-08-20 Merus N.V. Producing compositions comprising two or more antibodies.
CA3125380A1 (en) 2019-02-18 2020-08-27 Biocytogen Pharmaceuticals (Beijing) Co., Ltd. Genetically modified non-human animals with humanized immunoglobulin locus
TW202039578A (zh) 2019-03-29 2020-11-01 荷蘭商美勒斯公司 Cd3結合分子
TW202102544A (zh) * 2019-04-04 2021-01-16 日商小野藥品工業股份有限公司 雙特異性抗體
TW202108613A (zh) 2019-05-09 2021-03-01 荷蘭商美勒斯公司 用於多聚化蛋白質及其分離的變異區域
WO2021003149A1 (en) * 2019-07-01 2021-01-07 Trianni, Inc. Transgenic mammals and methods of use
KR20220030956A (ko) 2019-07-05 2022-03-11 오노 야꾸힝 고교 가부시키가이샤 Pd-1/cd3 이중 특이성 단백질에 의한 혈액암 치료
KR20220039720A (ko) 2019-07-30 2022-03-29 오노 야꾸힝 고교 가부시키가이샤 이중 특이성 항체
EP3772518A1 (en) 2019-08-07 2021-02-10 Merus N.V. Modified human variable domains
EP4017879A2 (en) 2019-08-19 2022-06-29 Merus N.V. Treatment of cancer with a combination of an antibody that binds lgr5 and egfr and a topoisomerase i inhibitor
GB201912008D0 (en) 2019-08-21 2019-10-02 Cambridge Entpr Ltd Antibody
JP2023504172A (ja) 2019-12-02 2023-02-01 リジェネロン・ファーマシューティカルズ・インコーポレイテッド ペプチド－ｍｈｃ ｉｉタンパク質構築物およびそれらの使用
CN115038497A (zh) 2019-12-24 2022-09-09 美勒斯公司 TGF-β-RII结合蛋白质
AU2021214622A1 (en) 2020-01-29 2022-07-21 Merus N.V. Means and method for modulating immune cell engaging effects.
EP4139357A1 (en) 2020-04-24 2023-03-01 Merus N.V. Treatment of cancers with an antibody that binds lgr5 and egfr
EP4158034A4 (en) * 2020-06-02 2024-07-03 Biocytogen Pharmaceuticals Beijing Co Ltd GENETICALLY MODIFIED NON-HUMAN ANIMALS HAVING A LIGHT-CHAIN IMMUNOGLOBULIN LOCUS
EP4211155A1 (en) 2020-09-11 2023-07-19 Regeneron Pharmaceuticals, Inc. Identification and production of antigen-specific antibodies
WO2022131912A1 (en) 2020-12-15 2022-06-23 Merus N.V. Treatment of cancers with an antibody that binds lgr5 and egfr
IL303626A (en) 2020-12-16 2023-08-01 Regeneron Pharma Mice expressing human FC alpha receptors
AU2021398976A1 (en) 2020-12-16 2023-07-06 Merus N.V. Multispecific antibodies for the treatment of cancer
WO2022131918A1 (en) 2020-12-18 2022-06-23 Merus N.V. Antibody composition
US20240317849A1 (en) 2020-12-23 2024-09-26 Regeneron Pharmaceuticals, Inc. Nucleic acids encoding anchor modified antibodies and uses thereof
MX2024004113A (es) 2021-10-06 2024-04-19 Merus Nv Tratamiento de canceres tratados con inhibidores de punto de control inmunitarios con alta expresion del receptor del factor de crecimiento epidermico (egfr) mediante el uso de un anticuerpo que se une al menos al receptor del factor de crecimiento epidermico (egfr).
CN118632872A (zh) 2022-01-25 2024-09-10 美勒斯公司 用于治疗癌症的组合疗法
AU2023231050A1 (en) 2022-03-07 2024-09-26 Merus N.V. Treatment with an antibody that binds egfr and cmet.
WO2023172133A1 (en) 2022-03-07 2023-09-14 Merus N.V. Combination therapy including antibodies that bind egfr and cmet
GB202216503D0 (en) 2022-11-05 2022-12-21 Quadrucept Bio Ltd Non-human vertebrates & cells
GB202217978D0 (en) 2022-11-30 2023-01-11 Petmedix Ltd Rodents expressing a common light chain
WO2024136658A1 (en) 2022-12-23 2024-06-27 Merus N.V. Combination treatment of cancers using an antibody that binds at least egfr and an immune checkpoint inhibitor
WO2024144401A1 (en) 2022-12-28 2024-07-04 Merus N.V. Treatment of cancer with a combination of an antibody that binds egfr and cytotoxic drugs

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4531078A (en) 1983-10-27 1985-07-23 Polaroid Corporation Control circuit for electronic flash
GB8909218D0 (en) 1989-04-22 1989-06-07 Medical Res Council Improvements in or relating to enhancers
ATE158021T1 (de) * 1990-08-29 1997-09-15 Genpharm Int Produktion und nützung nicht-menschliche transgentiere zur produktion heterologe antikörper
US7041871B1 (en) * 1995-10-10 2006-05-09 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5770429A (en) * 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
JPH07503132A (ja) * 1991-12-17 1995-04-06 ジェンファーム　インターナショナル，インコーポレイティド 異種抗体を産生することができるトランスジェニック非ヒト動物
JPH08140528A (ja) * 1993-12-03 1996-06-04 Genpharm Internatl Inc 異種抗体を産生することができるトランスジェニック非ヒト動物
US5885827A (en) * 1996-01-23 1999-03-23 The Regents Of The Universtiy Of California Eukaryotic high rate mutagenesis system
IL132560A0 (en) 1997-05-02 2001-03-19 Genentech Inc A method for making multispecific antibodies having heteromultimeric and common components
JP2002512624A (ja) 1997-05-21 2002-04-23 バイオベーション リミテッド 非免疫原性タンパク質の製造方法
EP1068524A4 (en) * 1998-03-30 2005-01-26 Res Dev Foundation CORTICOTROPHIN RELEASE FACTOR RECEPTOR DEFICIENT MOUSE 1
US20020028488A1 (en) * 2000-06-19 2002-03-07 Sujay Singh Transgenic avian species for making human and chimeric antibodies
US6596541B2 (en) * 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
CA2965865C (en) 2002-07-18 2021-10-19 Merus N.V. Recombinant production of mixtures of antibodies
EP1439234A1 (en) 2003-01-08 2004-07-21 ARTEMIS Pharmaceuticals GmbH Targeted transgenesis using the rosa26 locus
EP2395016A3 (en) 2003-05-30 2012-12-19 Merus B.V. Design and use of paired variable regions of specific binding molecules
DK1737971T3 (da) 2004-01-20 2017-11-13 Merus Nv Blandinger af bindingsproteiner
JP4487068B2 (ja) * 2004-10-12 2010-06-23 国立大学法人 岡山大学 細胞の遺伝子変異機能の制御による変異タンパク質の作製方法
JP2008538912A (ja) * 2005-04-29 2008-11-13 イナート・ファルマ 遺伝子導入動物および組換え抗体の製造方法
EP2505058A1 (en) * 2006-03-31 2012-10-03 Medarex, Inc. Transgenic animals expressing chimeric antibodies for use in preparing human antibodies
PL2041177T3 (pl) 2006-06-02 2012-09-28 Regeneron Pharma Przeciwciała o wysokim powinowactwie przeciw ludzkiemu receptorowi IL 6
DK2769992T3 (da) 2006-10-02 2021-03-22 Regeneron Pharma Humane antistoffer med høj affinitet for human IL-4-receptor
NO347649B1 (no) 2006-12-14 2024-02-12 Regeneron Pharma Humant antistoff eller antistoff fragment som spesifikt binder human deltaliknende ligand 4 (hDII4), nukleinsyremolekyl som koder for slike og vektor og vert-vektorsystemer, samt fremgangsmåte for fremstilling, sammensetning og anvendelse.
ES2906344T3 (es) * 2008-06-27 2022-04-18 Merus Nv Animal murino transgénico productor de anticuerpos
RU2724663C2 (ru) * 2010-02-08 2020-06-25 Ридженерон Фармасьютикалз, Инк. Мышь с общей легкой цепью

Also Published As

Publication number Publication date
CN105191863B (zh) 2020-12-22
JP6824807B2 (ja) 2021-02-03
WO2009157771A2 (en) 2009-12-30
KR102261586B1 (ko) 2021-06-08
DK3456190T3 (da) 2022-02-14
EP3456191A1 (en) 2019-03-20
SG10201914019YA (en) 2020-03-30
HRP20140139T1 (hr) 2014-03-14
ES2906344T3 (es) 2022-04-18
KR101990228B1 (ko) 2019-06-17
DK2556747T3 (da) 2021-02-15
CN105191863A (zh) 2015-12-30
ES2853226T3 (es) 2021-09-15
RU2559524C2 (ru) 2015-08-10
PT2556747T (pt) 2021-02-15
SG10201914027VA (en) 2020-03-30
JP7439201B2 (ja) 2024-02-27
MX2010014542A (es) 2011-04-05
KR20170066688A (ko) 2017-06-14
KR20190025068A (ko) 2019-03-08
JP7242611B2 (ja) 2023-03-20
EP3456190B1 (en) 2021-11-24
EP2556747B1 (en) 2020-12-02
JP2015156871A (ja) 2015-09-03
RU2731084C2 (ru) 2020-08-28
EP2147594A1 (en) 2010-01-27
EP3456190A1 (en) 2019-03-20
PT3456190T (pt) 2022-02-15
SI3456190T1 (sl) 2022-06-30
CA2729095C (en) 2018-12-04
AU2009263082A1 (en) 2009-12-30
JP2011525808A (ja) 2011-09-29
CN102123582A (zh) 2011-07-13
PL2147594T3 (pl) 2014-04-30
RS62925B1 (sr) 2022-03-31
EP3456193A1 (en) 2019-03-20
KR102306491B1 (ko) 2021-09-29
CN112481300A (zh) 2021-03-12
KR102112655B1 (ko) 2020-05-19
RU2020127564A (ru) 2022-02-21
WO2009157771A3 (en) 2010-02-18
EP2556747A3 (en) 2016-08-03
SI2147594T1 (sl) 2014-02-28
JP2024063038A (ja) 2024-05-10
AU2009263082B9 (en) 2014-07-17
KR20110029156A (ko) 2011-03-22
AU2009263082C1 (en) 2018-11-01
JP2022188085A (ja) 2022-12-20
RU2015119670A (ru) 2015-12-10
RS53146B (en) 2014-06-30
NZ590118A (en) 2012-10-26
CY1125119T1 (el) 2023-06-09
EP3456192A1 (en) 2019-03-20
ES2445193T3 (es) 2014-02-28
JP2017121256A (ja) 2017-07-13
ZA201009312B (en) 2011-08-31
HK1154463A1 (zh) 2012-04-27
SG10201408500RA (en) 2015-01-29
KR20200057093A (ko) 2020-05-25
CY1114830T1 (el) 2016-12-14
EP2147594B1 (en) 2013-11-13
RU2011102962A (ru) 2012-08-10
JP5749161B2 (ja) 2015-07-15
LT3456190T (lt) 2022-03-10
DK2147594T3 (da) 2014-02-10
RU2020127564A3 (hr) 2022-02-21
EP2556747A2 (en) 2013-02-13
CN102123582B (zh) 2015-09-30
JP2020191882A (ja) 2020-12-03
AU2009263082B2 (en) 2014-03-20
HUE057994T2 (hu) 2022-06-28
PL3456190T3 (pl) 2022-06-06
RU2015119670A3 (hr) 2019-01-22
KR20140127914A (ko) 2014-11-04
CA2729095A1 (en) 2009-12-30
PT2147594E (pt) 2014-02-17

Similar Documents

Publication Publication Date Title
HRP20220205T1 (hr) Transgena mišja životinja koja proizvodi antitijela
JP2020191882A5 (hr)
Santulli-Marotto et al. Autoreactive B cell regulation: peripheral induction of developmental arrest by lupus-associated autoantigens
HRP20120702T1 (hr) Ip-10 protutijela i njihova uporaba
ATE342282T1 (de) Humane monoklonale antikörper gegen prostata spezifisches membranantigen
SV2006001990A (es) Anticuerpos contra madcam
BRPI0921665A2 (pt) humanizado anti-il-6 anticorpos
BRPI0922479A2 (pt) Animais transgênicos não humanos que expressam os anticorpos humanizados e uso deles
WO2012148873A3 (en) Non-human animals expressing antibodies having a common light chain
RU2012128856A (ru) Мыши, которые производят антитела, имеющие только тяжелую цепь
LTPA2017040I1 (lt) Antikūnai prieš CD38 antigeną, skirti išsėtinės mielomos gydymui
PA8608401A1 (es) ANTICUERPOS DIRIGIDOS A c-MET
AR045563A1 (es) Anticuerpos dirigidos a m-csf
RU2008134517A (ru) Получение антител из одних тяжелых цепей в трансгенных животных
FI3653049T3 (fi) Polynukleotidit, jotka koodaavat jyrsijän vasta-aineita, joissa on ihmisen idiotyyppejä, ja näitä käsittävät eläimet
NO20040752L (no) Humane antistoffer spesifikke for interleukin 15 (IL-15)
PA8672101A1 (es) Anticuerpos anti-il-6, composiciones, métodos y usos
JP2011182801A5 (hr)
RS54468B1 (en) MONOCLONAL ANTIBODIES AGAINST CLAUDINE-18 FOR THE CANCER TREATMENT
BR112019008675A2 (pt) roedor, célula isolada de roedor, e, métodos de produção de um roedor e de um anticorpo em um roedor.
ATE540978T1 (de) Humane monoklonale antikörper gegen cd20
DK0942968T3 (da) Fuldt humane antistoffer, der binder EGFR
ATE553131T1 (de) Humane monoklonale antikörper gegen dendritische zellen
TR201909967T4 (tr) Histidin ile işlenmiş hafif zincirli antikorlar ve bunu üretmeye yönelik genetik olarak modifiye edilmiş insan olmayan hayvanlar.
Lee et al. Development and characterization of a CRISPR/Cas9-mediated RAG1 knockout chicken model lacking mature B and T cells