HRP20180506T1 - Pripravci i postupci za inhibiranje endogenih imunoglobinskih gena i proizvodnju transgenskih humanih idiotipnih protutijela - Google Patents

Pripravci i postupci za inhibiranje endogenih imunoglobinskih gena i proizvodnju transgenskih humanih idiotipnih protutijela Download PDF

Info

Publication number
HRP20180506T1
HRP20180506T1 HRP20180506TT HRP20180506T HRP20180506T1 HR P20180506 T1 HRP20180506 T1 HR P20180506T1 HR P20180506T T HRP20180506T T HR P20180506TT HR P20180506 T HRP20180506 T HR P20180506T HR P20180506 T1 HRP20180506 T1 HR P20180506T1
Authority
HR
Croatia
Prior art keywords
locus
meganuclease
human
artificial
cell
Prior art date
Application number
HRP20180506TT
Other languages
English (en)
Inventor
Ronald Buelow
Original Assignee
Open Monoclonal Technology, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40111008&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HRP20180506(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Open Monoclonal Technology, Inc. filed Critical Open Monoclonal Technology, Inc.
Publication of HRP20180506T1 publication Critical patent/HRP20180506T1/hr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/08—Antiallergic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8775—Murine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0609—Oocytes, oogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Communicable Diseases (AREA)
  • Pulmonology (AREA)
  • Transplantation (AREA)
  • Oncology (AREA)

Claims (16)

1. Postupak za ciljanu integraciju umjetnog imunoglobulinskog (Ig) mjesta u primateljskoj ne-humanoj zametnoj stanici, oplođenoj jajnoj stanici ili embrijima, koji obuhvaća: uvođenje u ne-ljudsku zametnu stanicu primatelja, gnojivo jajne stanice ili embrij mjesto specifične meganukleaze usmjerene na endogeni Ig lokus i transgenski vektor koji sadrži umjetni Ig lokus tako da umjetni Ig lokus integrira u genom primateljske ne-humane zametne stanice, oplođene oocite ili embrije ciljanom integracijom i zamjenjuje endogeni Ig lokus ili njihove dijelove homolognim rekombinacijom; gdje meganukleaza povećavaju učestalost homologne rekombinacije kroz dvostruko DNA cijepanje.
2. Postupak sukladno patentnom zahtjevu 1, naznačena time, da je meganukleaza osigurana u konstruktu ekspresije koju je moguće regulirati.
3. Postupak sukladno patentnom zahtjevu 2, naznačena time, da je meganukleaza operativno povezana s inducibilnim promotorom.
4. Postupak sukladno bilo kojem od patentnih zahtjeva 1 do 3, naznačen time, što se nadalje sastoji od odabira stanice u kojoj je umjetni Ig lokus integriran na ciljano mjesto i u kojem su regulativni konstrukti ekspresije meganukleaze integrirani u genom.
5. Postupak sukladno patentnom zahtjevu 4, naznačena time, da nadalje uključuje spajanje odabrane stanice sa stanicom za nuklearnu prijenosnu jedinicu koja nije ljudska, totipotentna i sposobna za tvorbu funkcionalnog novorođenog tkiva, kako bi se dobila rezultirajuća stanica.
6. Postupak sukladno patentnom zahtjevu 4 ili 5, naznačena time, da je enukleizirana stanična jedinica nuklearnog prijenosa oocit ili embrionalna matična stanica.
7. Postupak sukladno bilo kojem od patentnih zahtjeva 4 do 6, naznačen time, da nadalje sadrži: I. Injektirane odabrane stanice u razvoj ne-ljudskog embrija; ili II. kultiviranje rezultirajuće stanice u odgovarajućem mediju i prenošenje u nastalu stanicu u sinkronizirani primatelj za generiranje transgenične ne-humane životinje.
8. Postupak sukladno bilo kojem prethodnom patentnom zahtjevu, naznačen time, da umjetni Ig lokus sadrži fragmente ljudskih i ne-humanih Ig-lokusa.
9. Postupak sukladno bilo kojem prethodnom patentnom zahtjevu, naznačen time, da umjetni Ig lokus sadrži najmanje jedan segment humanog V gena.
10. Postupak sukladno bilo kojem prethodnom patentnom zahtjevu, naznačen time, da umjetni Ig lokus obuhvaća više segmenata imunoglobulinskog gena, pri čemu segmenti gena sadrže najmanje jedan segment genske regije V, jedan ili više segmenata J, jedan ili više segmenata gena D i jednu ili više konstanta regijskih gena.
11. Postupak sukladno bilo kojem prethodnom patentnom zahtjevu, naznačen time, da ciljana sekvenca meganukleaze je prisutna u ili proksimalno za segmente gena odabranih od: J segment gena; i genski segment konstantne regije.
12. Postupak sukladno bilo kojem prethodnom patentnom zahtjevu, naznačen time, da sukladno bilo kojem prethodnom patentnom zahtjevu, nadalje obuhvaća uporabu druge meganukleaze, pri čemu druga meganukleaza prepoznaje drugu ciljnu sekvencu meganukleaze prisutnu u ili proksimalnom endogenom Ig lokusu i selektivno cijepa endogeni Ig lokus zajedno s prvom meganukazom, ali na mjestu različitom od prve meganukleaze, čime se inaktivira najmanje jedan endogeni Ig lokus
13. Transgenska ne-humana životinja, koja se može dobiti metodom sukladno zahtjevu 9, naznačena time, da životinja sadrži: I. najmanje jedan regulirabilni konstrukt genomskog meganukleaza ekspresije; i II. umjetni Ig lokus koji zamjenjuje endogeni Ig lokus; pri čemu je životinja sposobna proizvesti protutijelo koje ima ljudski idiotip.
14. Transgenska životinja sukladno patentnog zahtjevu 13, naznačena time, da je ta životinja odabrana između ptice, glodara, i lasice.
15. Transgenska životinja sukladno patentnom zahtjevu 14, naznačeno time, da je ta životinja štakor.
16. In vitro primjena transgenskog vektora za ciljanu integraciju, pri čemu transgeni vektor sadrži induskcijsku ekspresijsku kontrolnu regiju koju je moguće koja je operabilno povezana s nukleinskom kiselinom koja kodira mjesno specifičnu meganukleazu koja cilja endogeni Ig lokus, pri čemu uporaba obuhvaća: uvođenje transgeničnog vektora u primateljsku ne-ljudsku zametnu stanicu, oplođene oocite ili embrij tako da se transgenski vektor integrira u genom zametne stanice, oplođene oocite ili embrija; i uvođenje transgenskog vektora koji sadrži umjetni Ig lokus u primateljsku ne-humanu zametnu stanicu, oplođeno oocite ili embrij, tako da se umjetni lokus integrira u genom primateljske ne-humane zametne stanice, oplođene oocite ili embrija ciljanom integracijom
HRP20180506TT 2007-06-01 2018-03-27 Pripravci i postupci za inhibiranje endogenih imunoglobinskih gena i proizvodnju transgenskih humanih idiotipnih protutijela HRP20180506T1 (hr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94161907P 2007-06-01 2007-06-01
US4432408P 2008-04-11 2008-04-11
EP12187787.2A EP2602323B1 (en) 2007-06-01 2008-05-30 Compositions and methods for inhibiting endogenous immunoglobin genes and producing transgenic human idiotype antibodies

Publications (1)

Publication Number Publication Date
HRP20180506T1 true HRP20180506T1 (hr) 2018-05-04

Family

ID=40111008

Family Applications (1)

Application Number Title Priority Date Filing Date
HRP20180506TT HRP20180506T1 (hr) 2007-06-01 2018-03-27 Pripravci i postupci za inhibiranje endogenih imunoglobinskih gena i proizvodnju transgenskih humanih idiotipnih protutijela

Country Status (22)

Country Link
US (6) US8703485B2 (hr)
EP (4) EP3382022A1 (hr)
JP (6) JP5823690B2 (hr)
KR (4) KR101886610B1 (hr)
CN (1) CN101784664B (hr)
AT (1) ATE522611T1 (hr)
AU (1) AU2008259939B2 (hr)
CA (2) CA2688834C (hr)
CY (1) CY1120383T1 (hr)
DK (3) DK2602323T3 (hr)
ES (3) ES2393826T3 (hr)
HK (1) HK1135138A1 (hr)
HR (1) HRP20180506T1 (hr)
HU (1) HUE037302T2 (hr)
IL (2) IL202302A (hr)
LT (1) LT2602323T (hr)
NZ (1) NZ581396A (hr)
PL (3) PL2336329T3 (hr)
PT (3) PT2152880E (hr)
SG (2) SG182144A1 (hr)
SI (1) SI2602323T1 (hr)
WO (1) WO2008151081A1 (hr)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI2311874T1 (sl) 2004-07-22 2017-12-29 Erasmus University Medical Center Rotterdam Department of Cell Biology and Genetics Vezavne molekule
AU2008259939B2 (en) 2007-06-01 2014-03-13 Open Monoclonal Technology, Inc. Compositions and methods for inhibiting endogenous immunoglobulin genes and producing transgenic human idiotype antibodies
WO2010026443A1 (en) * 2008-09-08 2010-03-11 Cellectis Meganuclease variants cleaving a dna target sequence from a glutamine synthetase gene and uses thereof
LT2346994T (lt) 2008-09-30 2022-03-10 Ablexis, Llc Knock-in pelė, skirta chimerinių antikūnų gamybai
EP3156494B8 (en) * 2008-12-04 2018-09-19 Sangamo Therapeutics, Inc. Genome editing in rats using zinc-finger nucleases
JP5827127B2 (ja) 2008-12-18 2015-12-02 エラスムス・ユニヴァーシティ・メディカル・センター・ロッテルダム ヒト化抗体を発現する非ヒトトランスジェニック動物及びその使用
GB0905023D0 (en) * 2009-03-24 2009-05-06 Univ Erasmus Medical Ct Binding molecules
US9445581B2 (en) 2012-03-28 2016-09-20 Kymab Limited Animal models and therapeutic molecules
PT2564695E (pt) 2009-07-08 2015-06-03 Kymab Ltd Modelos animais e moléculas terapêuticas
ES2595376T3 (es) 2009-12-10 2016-12-29 Regeneron Pharmaceuticals, Inc. Ratones que producen anticuerpos de cadena pesada
US9796788B2 (en) 2010-02-08 2017-10-24 Regeneron Pharmaceuticals, Inc. Mice expressing a limited immunoglobulin light chain repertoire
US20130045492A1 (en) 2010-02-08 2013-02-21 Regeneron Pharmaceuticals, Inc. Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain
RS55315B2 (sr) 2010-02-08 2020-08-31 Regeneron Pharma Miš sa zajedničkim lakim lancem
LT3248462T (lt) * 2010-03-31 2024-05-27 Ablexis, Llc Pelių genų inžinerija, skirta chimerinių antikūnų gamybai
CN103228130B (zh) * 2010-06-17 2016-03-16 科马布有限公司 动物模型及治疗分子
US10662256B2 (en) 2010-07-26 2020-05-26 Trianni, Inc. Transgenic mammals and methods of use thereof
US10881084B2 (en) 2010-07-26 2021-01-05 Trianni, Inc Transgenic animals and methods of use
US10793829B2 (en) 2010-07-26 2020-10-06 Trianni, Inc. Transgenic mammals and methods of use thereof
KR102434557B1 (ko) 2010-08-02 2022-08-23 리제너론 파아마슈티컬스, 인크. Ｖｌ 도메인을 포함하는 결합 단백질을 생성하는 마우스
US9920110B2 (en) 2011-03-09 2018-03-20 Cell Signaling Technology, Inc. Methods and reagents for creating monoclonal antibodies
BR112013025567B1 (pt) * 2011-04-27 2021-09-21 Amyris, Inc Métodos para modificação genômica
PT2739740T (pt) 2011-08-05 2020-01-09 Regeneron Pharma Cadeia leve universal humanizada de murganhos
JP2014533930A (ja) * 2011-09-19 2014-12-18 カイマブ・リミテッド 免疫グロブリン遺伝子多様性の操作およびマルチ抗体治療薬
ES2961613T3 (es) 2011-09-21 2024-03-12 Sangamo Therapeutics Inc Métodos y composiciones para la regulación de la expresión transgénica
EP2761008A1 (en) 2011-09-26 2014-08-06 Kymab Limited Chimaeric surrogate light chains (slc) comprising human vpreb
US10246509B2 (en) 2011-10-17 2019-04-02 Regeneron Pharmaceuticals, Inc. Restricted immunoglobulin heavy chain mice
US9253965B2 (en) 2012-03-28 2016-02-09 Kymab Limited Animal models and therapeutic molecules
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
US10251377B2 (en) 2012-03-28 2019-04-09 Kymab Limited Transgenic non-human vertebrate for the expression of class-switched, fully human, antibodies
PL2847335T3 (pl) * 2012-04-25 2019-01-31 Regeneron Pharmaceuticals, Inc. Celowanie dużymi wektorami do celowania wspomagane nukleazą
SG10201609277PA (en) 2012-06-12 2017-01-27 Regeneron Pharma Humanized non-human animals with restricted immunoglobulin heavy chain loci
KR20150023670A (ko) * 2012-06-12 2015-03-05 제넨테크, 인크. 조건적 녹아웃 대립유전자의 생성 방법 및 이를 위한 조성물
WO2014093908A2 (en) * 2012-12-14 2014-06-19 Omt, Inc. Polynucleotides encoding rodent antibodies with human idiotypes and animals comprising same
MY185881A (en) 2013-02-20 2021-06-14 Regeneron Pharma Non-human animals with modified immunoglobulin heavy chain sequences
PL2967012T3 (pl) 2013-03-14 2021-04-19 Erasmus University Medical Center Rotterdam Transgeniczne ssaki inne niż człowiek do wytwarzania przeciwciał
US10993420B2 (en) 2013-03-15 2021-05-04 Erasmus University Medical Center Production of heavy chain only antibodies in transgenic mammals
US9788534B2 (en) 2013-03-18 2017-10-17 Kymab Limited Animal models and therapeutic molecules
US20160040155A1 (en) * 2013-04-16 2016-02-11 University Of Washington Through Its Center For Commercialization Activating an alternative pathway for homology-directed repair to stimulate targeted gene correction and genome engineering
AU2014253942B9 (en) 2013-04-16 2020-08-13 Regeneron Pharmaceuticals, Inc. Targeted modification of rat genome
US9783618B2 (en) 2013-05-01 2017-10-10 Kymab Limited Manipulation of immunoglobulin gene diversity and multi-antibody therapeutics
US9783593B2 (en) 2013-05-02 2017-10-10 Kymab Limited Antibodies, variable domains and chains tailored for human use
US11707056B2 (en) 2013-05-02 2023-07-25 Kymab Limited Animals, repertoires and methods
CA2925723A1 (en) 2013-10-01 2015-04-09 Kymab Limited Animal models and therapeutic molecules
RU2725520C2 (ru) 2013-12-11 2020-07-02 Регенерон Фармасьютикалс, Инк. Способы и композиции для направленной модификации генома
US20150266976A1 (en) 2014-03-21 2015-09-24 Regeneron Pharmaceuticals, Inc. Vl antigen binding proteins exhibiting distinct binding characteristics
KR20210088756A (ko) 2014-03-21 2021-07-14 리제너론 파마슈티칼스 인코포레이티드 단일 도메인 결합 단백질을 생산하는 비-인간 동물
EA038470B1 (ru) 2014-04-30 2021-09-01 Ханол Биофарма Ко., Лтд. СВЯЗЫВАЮЩЕЕСЯ С FcRn АНТИТЕЛО ДЛЯ ЛЕЧЕНИЯ АУТОИММУННЫХ ЗАБОЛЕВАНИЙ
US10336825B2 (en) 2014-04-30 2019-07-02 Hanall Biopharma Co., Ltd. Antibody binding to FcRn for treating autoimmune diseases
GB201407852D0 (en) 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
KR102374379B1 (ko) 2014-06-06 2022-03-17 리제너론 파마슈티칼스 인코포레이티드 표적화된 좌를 변형시키는 방법 및 조성물
SG11201610633QA (en) 2014-06-26 2017-01-27 Regeneron Pharma Methods and compositions for targeted genetic modifications and methods of use
WO2016016442A1 (en) 2014-08-01 2016-02-04 INSERM (Institut National de la Santé et de la Recherche Médicale) An anti-cd45rc antibody for use as drug
KR102531016B1 (ko) 2014-11-21 2023-05-10 리제너론 파마슈티칼스 인코포레이티드 쌍 형성된 가이드 rna를 사용하는 표적화된 유전자 변형을 위한 방법 및 조성물
CA2971213C (en) 2014-12-19 2023-09-26 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modification through single-step multiple targeting
JP2018508224A (ja) 2015-03-19 2018-03-29 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 抗原を結合する軽鎖可変領域を選択する非ヒト動物
WO2016161446A1 (en) * 2015-04-03 2016-10-06 Dana-Farber Cancer Institute, Inc. Composition and methods of genome editing of b-cells
WO2017020291A1 (en) 2015-08-06 2017-02-09 Wuxi Biologics (Shanghai) Co. Ltd. Novel anti-pd-l1 antibodies
BR112018002824A2 (pt) 2015-08-11 2018-11-06 Open Monoclonal Tech Inc anticorpo, polinucleotídeo, vetor, célula, métodos para expressar o anticorpo, para tratar uma condição associada com pd-1 e para tratar uma condição em um sujeito, kit, composição farmacêutica e uso do anticorpo
AU2016311268B2 (en) * 2015-08-24 2023-02-02 Trianni, Inc. Enhanced production of immunoglobulins
WO2017095939A1 (en) 2015-12-03 2017-06-08 Trianni, Inc. Enhanced immunoglobulin diversity
AU2017213633B2 (en) 2016-02-04 2023-08-03 Trianni, Inc. Enhanced production of immunoglobulins
KR102598120B1 (ko) 2016-06-03 2023-11-07 리제너론 파마슈티칼스 인코포레이티드 외인성 말단 데옥시뉴클레오타이드 전달효소를 발현하는 비인간 동물
PT4050034T (pt) 2016-09-14 2024-05-27 Teneoone Inc Anticorpos de ligação a cd3
JP2020502991A (ja) 2016-09-20 2020-01-30 ウーシー バイオロジクス アイルランド リミテッド 新規抗ｐｃｓｋ９抗体
WO2018065552A1 (en) 2016-10-06 2018-04-12 Innate Pharma Anti-cd39 antibodies
IL300729A (en) 2016-12-21 2023-04-01 Teneobio Inc Anti-BCMA antibodies containing only heavy chains
EA201991514A1 (ru) 2016-12-21 2019-12-30 Сефалон, Инк. Антитела, которые специфически связываются с ил-15, и их применение
SG11201906540WA (en) 2017-01-19 2019-08-27 Open Monoclonal Tech Inc Human antibodies from transgenic rodents with multiple heavy chain immunoglobulin loci
WO2018237006A1 (en) 2017-06-20 2018-12-27 Teneoone, Inc. ANTIBODIES ONLY TO HEAVY ANTI-BCMA CHAINS
IL271194B2 (en) 2017-06-20 2024-10-01 Teneobio Inc Anti-BCMA antibodies containing only heavy chains
MX2020002612A (es) 2017-09-07 2020-07-13 Univ Res Inst Inc Augusta Anticuerpos de la proteina de muerte celular programada 1.
KR20200044094A (ko) 2017-09-13 2020-04-28 테네오바이오, 인코포레이티드 엑토효소에 결합하는 중쇄 항체
EP3498293A1 (en) 2017-12-15 2019-06-19 Institut National De La Sante Et De La Recherche Medicale (Inserm) Treatment of monogenic diseases with an anti-cd45rc antibody
WO2019126756A1 (en) 2017-12-22 2019-06-27 Teneobio, Inc. Heavy chain antibodies binding to cd22
SG11202006042SA (en) 2017-12-27 2020-07-29 Teneobio Inc Cd3-delta/epsilon heterodimer specific antibodies
CN116874591A (zh) 2018-03-24 2023-10-13 瑞泽恩制药公司 用于产生针对肽-mhc复合物的治疗性抗体的经过基因修饰的非人动物、其制造方法和用途
CA3102125A1 (en) * 2018-06-13 2019-12-19 Crystal Bioscience Inc. Transgenic chicken that makes antibodies with long cdr-h3s stabilized by multiple disulfide bridges and diversified by gene conversion
US12102070B2 (en) * 2018-06-13 2024-10-01 Crystal Bioscience Inc. Camelization of a human variable domain by gene conversion
SG11202012084WA (en) 2018-06-14 2021-01-28 Regeneron Pharma Non-human animals capable of dh-dh rearrangement in the immunoglobulin heavy chain coding sequences
SG11202011597RA (en) 2018-07-20 2020-12-30 Teneobio Inc Heavy chain antibodies binding to cd19
AU2019312576A1 (en) 2018-08-01 2021-02-25 Cephalon, LLC Anti-CXCR2 antibodies and uses thereof
EP3626265A1 (en) 2018-09-21 2020-03-25 INSERM (Institut National de la Santé et de la Recherche Médicale) Anti-human cd45rc antibodies and uses thereof
EP3914616A1 (en) 2019-01-23 2021-12-01 Encefa Cd31 competitors and uses thereof
CN117099741A (zh) 2019-02-18 2023-11-24 百奥赛图(北京)医药科技股份有限公司 具有人源化免疫球蛋白基因座的经遗传修饰的非人动物
JP2022538733A (ja) 2019-05-20 2022-09-06 インセルム（インスティチュート ナショナル デ ラ サンテ エ デ ラ リシェルシェ メディカル） 新規抗ｃｄ２５抗体
MX2021015337A (es) 2019-06-14 2022-01-18 Teneobio Inc Anticuerpos multiespecificos de cadena pesada que se unen a cd22 y cd3.
WO2021058795A2 (en) 2019-09-27 2021-04-01 Stark Labs Senescent cell-associated antigen-binding domains, antibodies and chimeric antigen receptors comprising the same, and uses thereof
CA3163549A1 (en) 2019-12-02 2021-06-10 Regeneron Pharmaceuticals, Inc. Peptide-mhc ii protein constructs and uses thereof
PE20230431A1 (es) 2020-04-29 2023-03-08 Teneobio Inc Anticuerpos de cadena pesada multiespecificos con regiones constantes de cadena pesada modificadas
CN115667532A (zh) 2020-06-02 2023-01-31 百奥赛图(北京)医药科技股份有限公司 具有共有轻链免疫球蛋白基因座的经遗传修饰的非人动物
TW202229328A (zh) 2020-09-11 2022-08-01 美商再生元醫藥公司 抗原特異性抗體之鑑定及產生
US20240002522A1 (en) 2020-11-20 2024-01-04 Inserm (Institut National De La Sante Et De La Recherche Medicale Anti-cd25 antibodies
JP2024504547A (ja) 2020-11-20 2024-02-01 インセルム（インスティチュート ナショナル デ ラ サンテ エ デ ラ リシェルシェ メディカル） 抗ｃｄ２５抗体
KR20230147048A (ko) 2020-12-16 2023-10-20 리제너론 파마슈티칼스 인코포레이티드 인간화 Fc 알파 수용체를 발현하는 마우스
US20240317849A1 (en) 2020-12-23 2024-09-26 Regeneron Pharmaceuticals, Inc. Nucleic acids encoding anchor modified antibodies and uses thereof
WO2023170207A1 (en) 2022-03-09 2023-09-14 Alderaan Biotechnology Anti-cd160 transmembrane isoform antibodies
AU2023281650A1 (en) 2022-05-30 2024-10-17 Hanall Biopharma Co., Ltd. Anti-fcrn antibody or antigen binding fragment thereof with improved stability
WO2024062019A1 (en) 2022-09-21 2024-03-28 Synabs Anti-ccr8 antibodies and uses thereof

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4977081A (en) 1987-05-04 1990-12-11 Adi Diagnostics, Inc. Stable rabbit-mouse hybridomas and secretion products thereof
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US5175384A (en) 1988-12-05 1992-12-29 Genpharm International Transgenic mice depleted in mature t-cells and methods for making transgenic mice
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5574205A (en) * 1989-07-25 1996-11-12 Cell Genesys Homologous recombination for universal donor cells and chimeric mammalian hosts
DE69120146T2 (de) 1990-01-12 1996-12-12 Cell Genesys Inc Erzeugung xenogener antikörper
US6075181A (en) * 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6657103B1 (en) * 1990-01-12 2003-12-02 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6150584A (en) * 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6713610B1 (en) * 1990-01-12 2004-03-30 Raju Kucherlapati Human antibodies derived from immunized xenomice
US6673986B1 (en) * 1990-01-12 2004-01-06 Abgenix, Inc. Generation of xenogeneic antibodies
WO1992001043A1 (fr) 1990-07-10 1992-01-23 Nkk Corporation Hydridomes produisant une immunoglobuline g aviaire specifique
US5612205A (en) 1990-08-29 1997-03-18 Genpharm International, Incorporated Homologous recombination in mammalian cells
US5770429A (en) 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US7041871B1 (en) * 1995-10-10 2006-05-09 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
DK0814159T3 (da) 1990-08-29 2005-10-24 Genpharm Int Transgene, ikke-humane dyr, der er i stand til at danne heterologe antistoffer
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
DE4119749A1 (de) 1991-06-15 1992-12-17 Claas Ohg Verfahren zum entholzen von flachs und flachsaufbereitungsmaschine zur durchfuehrung dieses verfahrens
CA2140638C (en) 1992-07-24 2010-05-04 Raju Kucherlapati Generation of xenogeneic antibodies
EP0583980A1 (en) 1992-08-20 1994-02-23 Eli Lilly And Company Method for generating monoclonal antibodies from rabbits
EP2192131A1 (en) 1992-08-21 2010-06-02 Vrije Universiteit Brussel Immunoglobulins devoid of light chains
US6765087B1 (en) 1992-08-21 2004-07-20 Vrije Universiteit Brussel Immunoglobulins devoid of light chains
US6005079A (en) 1992-08-21 1999-12-21 Vrije Universiteit Brussels Immunoglobulins devoid of light chains
US5795974A (en) 1993-12-10 1998-08-18 University Of Utah Research Foundation Mycoplasma arthritidis superantigen
US6130364A (en) 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US6091001A (en) 1995-03-29 2000-07-18 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
EP1709970A1 (en) 1995-04-27 2006-10-11 Abgenix, Inc. Human antibodies against EGFR, derived from immunized xenomice
AU7326796A (en) 1995-10-30 1997-05-22 Spectral Diagnostics Inc. Stable chicken b-cell line and method of use thereof
US5716081A (en) 1996-03-11 1998-02-10 Automotive Products (Usa), Inc. Spring clip for quick connect coupling
CA2273194C (en) * 1996-12-03 2011-02-01 Abgenix, Inc. Transgenic mammals having human ig loci including plural vh and vk regions and antibodies produced therefrom
GB9823930D0 (en) 1998-11-03 1998-12-30 Babraham Inst Murine expression of human ig\ locus
AU2391000A (en) * 1998-12-31 2000-07-31 J. David Gladstone Institutes, The Transgenic rodents and rodent cell lines expressing hiv co-receptors
US6833268B1 (en) * 1999-06-10 2004-12-21 Abgenix, Inc. Transgenic animals for producing specific isotypes of human antibodies via non-cognate switch regions
AU7676300A (en) * 1999-10-12 2001-04-23 Cambridge Antibody Technology Limited Human anti-adipocyte monoclonal antibodies and their use
AU5561301A (en) * 2000-04-24 2001-11-20 American Home Prod Transgenic animal
IL154751A0 (en) * 2000-08-03 2003-10-31 Humanized antibodies, recombination vectors, transgenic vectors and methods for the preparation of humanized antibodies from transgenic animals
US6596541B2 (en) 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US6586251B2 (en) 2000-10-31 2003-07-01 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
EP1916303B1 (en) * 2000-11-30 2013-02-27 Medarex, Inc. Nucleic acids encoding rearranged human immunoglobulin sequences from transgenic transchromosomal mice
GB0110029D0 (en) 2001-04-24 2001-06-13 Grosveld Frank Transgenic animal
GB0115256D0 (en) 2001-06-21 2001-08-15 Babraham Inst Mouse light chain locus
WO2003016495A2 (en) 2001-08-20 2003-02-27 Merck & Co., Inc. Transgenic rodents as animal models for modulation of b1 bradykinin receptor protein
ATE466088T1 (de) * 2001-09-14 2010-05-15 Cellectis Zufällige integration von polynukleotide nach in vivo linearisierung
ES2645698T3 (es) * 2001-11-30 2017-12-07 Amgen Fremont Inc. Animales transgénicos que portan genes de cadena ligera de Ig humana
CA2479153C (en) 2002-03-15 2015-06-02 Cellectis Hybrid and single chain meganucleases and use thereof
ATE531796T1 (de) * 2002-03-21 2011-11-15 Sangamo Biosciences Inc Verfahren und zusammensetzungen zur verwendung von zinkfinger-endonukleasen zur verbesserung der homologen rekombination
GB0228210D0 (en) 2002-12-03 2003-01-08 Babraham Inst Single chain antibodies
JP4966006B2 (ja) * 2003-01-28 2012-07-04 セレクティス カスタムメイドメガヌクレアーゼおよびその使用
WO2004072115A2 (en) * 2003-02-05 2004-08-26 Therapeutic Human Polyclonals, Inc. Suppression of endogenous immunoglobulin expression in transgenic non-human animals expressing humanized or human antibodies
GB2398784B (en) 2003-02-26 2005-07-27 Babraham Inst Removal and modification of the immunoglobulin constant region gene cluster of a non-human mammal
RS20050934A (en) * 2003-06-16 2008-04-04 Celltech R. & D. Inc., Antibodies specific for sclerostin and methods fo r increasing bone mineralization
JP2007537704A (ja) * 2003-07-15 2007-12-27 セラピューティック ヒューマン ポリクローナルズ， インコーポレイテッド ヒト化免疫グロブリン遺伝子座
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
WO2005019463A1 (en) * 2003-08-11 2005-03-03 Therapeutic Human Polyclonals, Inc. Improved transgenesis with humanized immunoglobulin loci
WO2005038001A2 (en) * 2003-10-14 2005-04-28 Therapeutic Human Polyclonals, Inc. Improved transgenesis by sperm-mediated gene transfer
KR20120091471A (ko) 2004-03-04 2012-08-17 도쿠리츠교세이호진 고쿠리츠간켄큐센터 래트 배아 줄기 세포
SI2311874T1 (sl) 2004-07-22 2017-12-29 Erasmus University Medical Center Rotterdam Department of Cell Biology and Genetics Vezavne molekule
KR20070072608A (ko) * 2004-10-22 2007-07-04 레비비코르 인코포레이션 유전자 변형된 면역계를 가지는 유제류
CA2584814A1 (en) * 2004-10-22 2006-05-04 Therapeutic Human Polyclonals, Inc. Suppression of endogenous immunoglobulin expression in non-human transgenic animals
WO2006093337A1 (ja) * 2005-03-03 2006-09-08 Takeda Pharmaceutical Company Limited 癌の予防・治療剤
ATE466933T1 (de) 2005-03-15 2010-05-15 Cellectis I-crei-meganuklease-varianten mit modifizierter spezifität sowie verfahren zu ihrer herstellung und verwendung
WO2006097784A1 (en) 2005-03-15 2006-09-21 Cellectis I-crei meganuclease variants with modified specificity, method of preparation and uses thereof
WO2007060495A1 (en) 2005-10-25 2007-05-31 Cellectis I-crei homing endonuclease variants having novel cleavage specificity and use thereof
US7491866B2 (en) 2005-11-09 2009-02-17 Board Of Regents University Of Texas System Transgenic rats and spermatogonial stem cells
KR101481843B1 (ko) 2006-01-25 2015-01-12 에라스무스 유니버시티 메디컬 센터 로테르담 형질전환 동물 내에서의 중쇄만의 항체의 생성
JP5514539B2 (ja) 2006-03-31 2014-06-04 メダレックス・リミテッド・ライアビリティ・カンパニー ヒト抗体の調製に用いるためのキメラ抗体を発現するトランスジェニック動物
AU2008259939B2 (en) * 2007-06-01 2014-03-13 Open Monoclonal Technology, Inc. Compositions and methods for inhibiting endogenous immunoglobulin genes and producing transgenic human idiotype antibodies
MY185881A (en) * 2013-02-20 2021-06-14 Regeneron Pharma Non-human animals with modified immunoglobulin heavy chain sequences

Also Published As

Publication number Publication date
DK2152880T3 (da) 2011-11-28
CA2688834A1 (en) 2008-12-11
JP6220827B2 (ja) 2017-10-25
ES2664218T3 (es) 2018-04-18
EP3382022A1 (en) 2018-10-03
EP2602323A1 (en) 2013-06-12
EP2152880A4 (en) 2010-06-16
ATE522611T1 (de) 2011-09-15
KR101886610B1 (ko) 2018-08-09
PL2336329T3 (pl) 2013-04-30
JP6712254B2 (ja) 2020-06-17
HUE037302T2 (hu) 2018-08-28
ES2372718T3 (es) 2012-01-25
WO2008151081A1 (en) 2008-12-11
JP2022118217A (ja) 2022-08-12
PT2336329E (pt) 2012-12-24
US9388233B2 (en) 2016-07-12
PL2602323T3 (pl) 2018-06-29
US20190169270A1 (en) 2019-06-06
IL202302A (en) 2013-07-31
NZ581396A (en) 2012-07-27
DK2602323T3 (en) 2018-04-16
US8703485B2 (en) 2014-04-22
CN101784664A (zh) 2010-07-21
PT2602323T (pt) 2018-04-02
KR102096731B1 (ko) 2020-04-02
US10072069B2 (en) 2018-09-11
US20090098134A1 (en) 2009-04-16
JP2010529042A (ja) 2010-08-26
CN102850452A (zh) 2013-01-02
WO2008151081A8 (en) 2010-02-04
JP2020125360A (ja) 2020-08-20
IL220209A0 (en) 2012-07-31
JP5823690B2 (ja) 2015-11-25
KR101703299B1 (ko) 2017-02-06
EP2152880A1 (en) 2010-02-17
EP2152880B1 (en) 2011-08-31
KR20150101475A (ko) 2015-09-03
IL202302A0 (en) 2011-08-01
CA3054224A1 (en) 2008-12-11
IL220209A (en) 2013-11-28
SI2602323T1 (en) 2018-05-31
KR20170016518A (ko) 2017-02-13
AU2008259939B2 (en) 2014-03-13
DK2336329T3 (da) 2013-01-07
US8907157B2 (en) 2014-12-09
AU2008259939A1 (en) 2008-12-11
PL2152880T3 (pl) 2012-03-30
EP2336329A1 (en) 2011-06-22
KR101661357B1 (ko) 2016-09-29
CY1120383T1 (el) 2019-07-10
US20210253673A1 (en) 2021-08-19
EP2336329B1 (en) 2012-10-10
EP2336329B8 (en) 2012-11-21
US20160311888A1 (en) 2016-10-27
JP2014027947A (ja) 2014-02-13
JP2015204840A (ja) 2015-11-19
JP2018000201A (ja) 2018-01-11
CA2688834C (en) 2020-01-07
US20120090041A1 (en) 2012-04-12
ES2393826T3 (es) 2012-12-28
SG182144A1 (en) 2012-07-30
CN101784664B (zh) 2013-04-10
US20100212035A1 (en) 2010-08-19
EP2602323B1 (en) 2018-02-28
HK1135138A1 (en) 2010-05-28
PT2152880E (pt) 2011-12-15
KR20100037027A (ko) 2010-04-08
KR20180090395A (ko) 2018-08-10
SG10201509853UA (en) 2015-12-30
LT2602323T (lt) 2018-04-10

Similar Documents

Publication Publication Date Title
HRP20180506T1 (hr) Pripravci i postupci za inhibiranje endogenih imunoglobinskih gena i proizvodnju transgenskih humanih idiotipnih protutijela
Kuroiwa et al. Cloned transchromosomic calves producing human immunoglobulin
Dyck et al. Making recombinant proteins in animals–different systems, different applications
Cooper et al. Innovative approaches to genome editing in avian species
ES2827482T3 (es) Uso de un ratón para producir anticuerpos híbridos que contienen regiones variables humanas y regiones constantes de ratón como fuente de ADN que codifica una región variable humana de dicho anticuerpo
BR112014026294B1 (pt) Método para modificar um lócus genômico alvo em uma célula tronco embrionária (es) de camundongo
CN111471719A (zh) 有效的非减数分裂等位基因渐渗
KR20200093570A (ko) 조작된 면역글로불린 람다 경쇄를 갖는 비-인간 동물 및 그의 용도
Tizard et al. Potential benefits of gene editing for the future of poultry farming
CN105658050A (zh) 用于制备具有可分选精子的动物的遗传技术
CN109112159A (zh) 基于Cas9介导的定点整合FABP4基因和MSTN基因点突变打靶载体及重组细胞
Jones Bicentennial: Genetic engineering of a mouse: Dr. Frank Ruddle and somatic cell genetics
CA2501415A1 (en) Method of selecting cells for somatic cell nuclear transfer
CA2300972A1 (en) Use of mariner transposan in the production of transgenic animals
Zou et al. Dominant expression of a 1.3 Mb human Igk locus replacing mouse light chain production
JP7426120B2 (ja) ノックイン細胞の作製方法
KR20210106412A (ko) 유전적으로 변형된 불임 조류 및 이의 재구성 방법
CN106978416A (zh) 一种基因定位整合表达系统及其应用
US20190045761A1 (en) Inducible disease models methods of making them and use in tissue complementation
Duncan et al. Chromosomal engineering
Cyriac et al. Transgenic chicken: methods and applications.
CN114606270B (zh) 一种Mia3条件性基因敲除小鼠模型的构建方法
Guo et al. Multiplexed genome engineering for porcine fetal fibroblasts with gRNA–tRNA arrays based on CRISPR/Cas9
KR101724805B1 (ko) 유전자 적중 물질을 이용한 조류 유전자 편집방법 및 유전자 편집 조류
US20240090480A1 (en) Split-intein methods and compositions for generation of single sex offspring