HK1198661A1 - 核酸編碼反應 - Google Patents

核酸編碼反應

Info

Publication number
HK1198661A1
HK1198661A1 HK14112205.0A HK14112205A HK1198661A1 HK 1198661 A1 HK1198661 A1 HK 1198661A1 HK 14112205 A HK14112205 A HK 14112205A HK 1198661 A1 HK1198661 A1 HK 1198661A1
Authority
HK
Hong Kong
Prior art keywords
nucleic acid
acid encoding
encoding reactions
reactions
nucleic
Prior art date
Application number
HK14112205.0A
Other languages
English (en)
Inventor
梅甘．安德森
陳佩林
布賴恩．福勒
羅伯特．
．瓊斯
菲奧娜．卡佩爾
羅納德．列波夫斯基
安德魯．梅
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of HK1198661A1 publication Critical patent/HK1198661A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK14112205.0A 2011-05-20 2014-12-03 核酸編碼反應 HK1198661A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161519348P 2011-05-20 2011-05-20
PCT/US2012/038894 WO2012162267A2 (en) 2011-05-20 2012-05-21 Nucleic acid encoding reactions

Publications (1)

Publication Number Publication Date
HK1198661A1 true HK1198661A1 (zh) 2015-05-22

Family

ID=47218012

Family Applications (1)

Application Number Title Priority Date Filing Date
HK14112205.0A HK1198661A1 (zh) 2011-05-20 2014-12-03 核酸編碼反應

Country Status (6)

Country Link
US (3) US9074204B2 (zh)
EP (1) EP2710172B1 (zh)
CN (2) CN103890245B (zh)
HK (1) HK1198661A1 (zh)
SG (2) SG10201605049QA (zh)
WO (1) WO2012162267A2 (zh)

Families Citing this family (190)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2313143T3 (es) 2005-04-06 2009-03-01 Maurice Stroun Metodo para el diagnostico de cancer mediante la deteccion de adn y arn circulantes.
US8697363B2 (en) * 2008-08-26 2014-04-15 Fluidigm Corporation Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags
SG10201402770YA (en) 2009-04-02 2014-08-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
WO2011056643A2 (en) * 2009-10-27 2011-05-12 The Regents Of The University Of Michigan Microfluidic platform for discrete cell assay
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
CN110878345A (zh) 2010-09-21 2020-03-13 安捷伦科技有限公司 通过分子计数提高等位基因调用的置信度
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
CA2826131C (en) 2011-02-02 2019-11-05 Jay Ashok Shendure Massively parallel continguity mapping
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
CN103890245B (zh) 2011-05-20 2020-11-17 富鲁达公司 核酸编码反应
BR112013033462A2 (pt) * 2011-06-27 2017-03-01 Univ Florida método para a redução de complexidade do genoma e detecção de polimorfismo
AU2012304328B2 (en) 2011-09-09 2017-07-20 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
LT3363901T (lt) * 2012-02-17 2021-04-12 Fred Hutchinson Cancer Research Center Kompozicijos ir būdai, skirti tiksliam mutacijų nustatymui
ES2776673T3 (es) 2012-02-27 2020-07-31 Univ North Carolina Chapel Hill Métodos y usos para etiquetas moleculares
GB2504240B (en) 2012-02-27 2015-05-27 Cellular Res Inc Compositions and kits for molecular counting of nucleic acids
US9670529B2 (en) 2012-02-28 2017-06-06 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
WO2013134261A1 (en) 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
EP2825675B1 (en) * 2012-03-13 2017-12-27 Patel, Abhijit Ajit Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
US9487828B2 (en) 2012-05-10 2016-11-08 The General Hospital Corporation Methods for determining a nucleotide sequence contiguous to a known target nucleotide sequence
CN104471077B (zh) 2012-05-21 2017-05-24 富鲁达公司 颗粒群的单颗粒分析
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
GB2519906B (en) 2012-08-13 2017-02-08 Univ California Methods for detecting target nucleic acids in sample lysate droplets
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN110872617A (zh) 2012-09-04 2020-03-10 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2914379A1 (en) * 2012-11-01 2015-09-09 The Charles Stark Draper Laboratory, Inc. Ex vivo microfluidic analysis of biologic samples
AU2013337280B2 (en) * 2012-11-05 2018-11-08 Takara Bio Usa, Inc. Barcoding nucleic acids
US20150322508A1 (en) * 2012-12-28 2015-11-12 Fleury S.A. Method for complete tracking of a set of biological samples containing dna or rna through molecular barcode identification during laboratorial workflow and kit for collecting biological samples containing dna or rna
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
ES2724824T3 (es) * 2013-03-13 2019-09-16 Illumina Inc Métodos para la secuenciación de ácidos nucleicos
WO2014152155A1 (en) * 2013-03-14 2014-09-25 The Broad Institute, Inc. Massively multiplexed rna sequencing
US9416387B2 (en) 2013-03-15 2016-08-16 Theranos, Inc. Nucleic acid amplification
US9725760B2 (en) 2013-03-15 2017-08-08 Theranos, Inc. Nucleic acid amplification
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
EP2970960B1 (en) 2013-03-15 2019-03-13 Theranos IP Company, LLC Nucleic acid amplification
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
EP3578667B1 (en) 2013-04-17 2023-10-04 Pioneer Hi-Bred International, Inc. Methods for characterizing dna sequence composition in a genome
EP2805769A1 (en) * 2013-05-24 2014-11-26 European Molecular Biology Laboratory Methods for nano-scale single cell analysis
SG10201806428YA (en) 2013-06-28 2018-08-30 X Body Inc Target antigen discovery, phenotypic screens and use thereof for identification of target cell specific target epitopes
CN105637097A (zh) 2013-08-05 2016-06-01 特韦斯特生物科学公司 从头合成的基因文库
JP6545682B2 (ja) 2013-08-28 2019-07-17 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 大規模並列単一細胞分析
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
CN105849264B (zh) 2013-11-13 2019-09-27 纽亘技术公司 用于鉴别重复测序读数的组合物和方法
CA2933387C (en) 2013-12-11 2023-05-02 The Regents Of The University Of California Methods for labeling dna fragments to reconstruct physical linkage and phase
WO2015100427A1 (en) 2013-12-28 2015-07-02 Guardant Health, Inc. Methods and systems for detecting genetic variants
AU2015209718B2 (en) * 2014-01-25 2021-03-25 Psomagen, Inc. Method and system for microbiome analysis
EP4219744A3 (en) * 2014-01-27 2023-08-30 The General Hospital Corporation Methods of preparing nucleic acids for sequencing
WO2015112948A2 (en) * 2014-01-27 2015-07-30 Iafrate Anthony John Methods for determining a nucleotide sequence
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN105087756A (zh) * 2014-04-23 2015-11-25 北京贝瑞和康生物技术有限公司 一种无创检测胎儿耳聋致病基因突变的方法及试剂盒
SG11201609055RA (en) * 2014-05-08 2016-11-29 Fluidigm Corp Integrated single cell sequencing
CN107075509B (zh) * 2014-05-23 2021-03-09 数字基因公司 通过数字化转座子的单倍体组测定
GB201409282D0 (en) * 2014-05-23 2014-07-09 Univ Sydney Tech Sequencing process
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
SG11201610910QA (en) * 2014-06-30 2017-01-27 Illumina Inc Methods and compositions using one-sided transposition
EP3428185A1 (en) * 2014-07-28 2019-01-16 Fundacio Institut mar d'Investigacions Médiques (IMIM) Mutations in the extracellular domain iii of epidermal growth factor receptor gene
EP3174980A4 (en) 2014-08-01 2018-01-17 Dovetail Genomics, LLC Tagging nucleic acids for sequence assembly
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
CA2955967A1 (en) * 2014-08-14 2016-02-18 Abbott Molecular Inc. Multifunctional oligonucleotides
WO2016037358A1 (zh) 2014-09-12 2016-03-17 深圳华大基因科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
EP3209419A4 (en) 2014-10-22 2018-10-03 The Regents of The University of California High definition microdroplet printer
CN104313172A (zh) * 2014-11-06 2015-01-28 中国海洋大学 一种大量样本同时分型的方法
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
EP3237636B1 (en) * 2014-12-22 2020-10-21 Anapa Biotech A/S Dual quenching assay for multiplex detection of target nucleic acids
WO2016115457A1 (en) 2015-01-16 2016-07-21 California Institute Of Technology Methods and devices for micro-isolation, extraction, and/or analysis of microscale components in an array
US10760120B2 (en) * 2015-01-23 2020-09-01 Qiagen Sciences, Llc High multiplex PCR with molecular barcoding
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
CN115011670A (zh) 2015-02-04 2022-09-06 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
EP3725893A1 (en) * 2015-02-10 2020-10-21 Illumina, Inc. Compositions for analyzing cellular components
WO2016134034A1 (en) 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
CN107250379B (zh) 2015-02-19 2021-12-28 贝克顿迪金森公司 结合蛋白质组信息和基因组信息的高通量单细胞分析
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
WO2016138490A1 (en) * 2015-02-27 2016-09-01 Fluidigm Corporation Single-cell nucleic acids for high-throughput studies
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
US10011869B2 (en) 2015-03-06 2018-07-03 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
ES2934982T3 (es) * 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
US11746367B2 (en) 2015-04-17 2023-09-05 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US10385387B2 (en) 2015-04-20 2019-08-20 Pacific Biosciences Of California, Inc. Methods for selectively amplifying and tagging nucleic acids
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
CN107849603B (zh) 2015-04-24 2022-01-28 阿提拉生物系统公司 利用有限核苷酸组成的引物扩增
BR122023026077A2 (pt) * 2015-05-27 2024-02-27 Quest Diagnostics Investments Incorporated Métodos para detecção de pelo menos uma mutação em uma pluralidade de genes relacionados ao câncer, selecionar um indivíduo para tratamento e prever a probabilidade de capacidade de resposta ao tratamento
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
GB2539675B (en) * 2015-06-23 2017-11-22 Cs Genetics Ltd Libraries of multimeric barcoding reagents and kits thereof for labelling nucleic acids for sequencing
US10280449B2 (en) * 2015-06-29 2019-05-07 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
CA2991401C (en) 2015-07-07 2023-12-05 Pillar Biosciences Inc. Method for reducing primer-dimer amplification
JP6847496B2 (ja) * 2015-08-06 2021-03-24 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 単一プローブプライマー伸長による標的濃縮
US11302416B2 (en) 2015-09-02 2022-04-12 Guardant Health Machine learning for somatic single nucleotide variant detection in cell-free tumor nucleic acid sequencing applications
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation BANKS OF OLIGONUCLEIC ACID VARIANTS AND SYNTHESIS THEREOF
KR20180058772A (ko) 2015-09-22 2018-06-01 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
WO2017070123A1 (en) 2015-10-19 2017-04-27 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
WO2017074934A1 (en) * 2015-10-28 2017-05-04 Wafergen, Inc. Single cell capture with capture chips
JP7064439B2 (ja) * 2015-11-04 2022-05-10 アトレカ インコーポレイテッド 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
CN108603180A (zh) 2015-11-25 2018-09-28 豪夫迈·罗氏有限公司 聚合酶复合体的纯化
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
CN117174167A (zh) 2015-12-17 2023-12-05 夸登特健康公司 通过分析无细胞dna确定肿瘤基因拷贝数的方法
CA3014911A1 (en) 2016-02-23 2017-08-31 Dovetail Genomics, Llc Generation of phased read-sets for genome assembly and haplotype phasing
US9976181B2 (en) 2016-03-25 2018-05-22 Karius, Inc. Synthetic nucleic acid spike-ins
WO2017181146A1 (en) 2016-04-14 2017-10-19 Guardant Health, Inc. Methods for early detection of cancer
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
AU2017261189B2 (en) 2016-05-02 2023-02-09 Becton, Dickinson And Company Accurate molecular barcoding
EP3954771A1 (en) 2016-05-13 2022-02-16 Dovetail Genomics, LLC Recovering long-range linkage information from preserved samples
WO2017201331A2 (en) * 2016-05-20 2017-11-23 Trovagene, Inc. Oligonucleotide sequences for detection of low abundance target sequences and kits thereof
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CN107557355A (zh) * 2016-07-01 2018-01-09 Pgi股份有限公司 建构环状模板和检测dna分子的方法
CA3030430A1 (en) 2016-07-15 2018-01-18 The Regents Of The University Of California Methods of producing nucleic acid libraries
JP2019521713A (ja) * 2016-07-22 2019-08-08 ヌクレオトレイス プロプライアタリー リミティド 核酸配列の増幅方法
WO2018027048A1 (en) * 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
KR102212257B1 (ko) 2016-08-22 2021-02-04 트위스트 바이오사이언스 코포레이션 드 노보 합성된 핵산 라이브러리
GB2569252A (en) * 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
WO2018057526A2 (en) 2016-09-21 2018-03-29 Twist Bioscience Corporation Nucleic acid based data storage
KR102522023B1 (ko) 2016-09-26 2023-04-17 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
KR102344635B1 (ko) 2016-09-30 2021-12-31 가던트 헬쓰, 인크. 무세포 핵산의 다중-해상도 분석 방법
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
IL266197B2 (en) 2016-10-24 2024-03-01 Geneinfosec Inc Hiding information contained within nucleic acids
EP3539035B1 (en) 2016-11-08 2024-04-17 Becton, Dickinson and Company Methods for expression profile classification
JP7228510B2 (ja) 2016-11-08 2023-02-24 ベクトン・ディキンソン・アンド・カンパニー 細胞標識分類の方法
EA201991262A1 (ru) 2016-12-16 2020-04-07 Твист Байосайенс Корпорейшн Библиотеки вариантов иммунологического синапса и их синтез
AU2017382905A1 (en) 2016-12-21 2019-07-04 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
JP6234542B1 (ja) 2016-12-27 2017-11-22 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ 胎児細胞由来染色体ｄｎａの取得方法
WO2018132610A1 (en) 2017-01-13 2018-07-19 Cellular Research, Inc. Hydrophilic coating of fluidic channels
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CN118116478A (zh) 2017-02-22 2024-05-31 特韦斯特生物科学公司 基于核酸的数据存储
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
EP3606669A1 (en) 2017-04-03 2020-02-12 The Charles Stark Draper Laboratory, Inc. Microfluidic system for evaluation of chemotherapeutic and immunotherapeutic drugs
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
KR20240013290A (ko) 2017-06-12 2024-01-30 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
EP3642363A1 (en) * 2017-06-20 2020-04-29 Bio-Rad Laboratories, Inc. Mda using bead oligonucleotide
US11505826B2 (en) * 2017-07-12 2022-11-22 Agilent Technologies, Inc. Sequencing method for genomic rearrangement detection
JP2020532964A (ja) 2017-08-11 2020-11-19 アティラ バイオシステムズ インコーポレイテッドＡｔｉｌａ Ｂｉｏｓｙｓｔｅｍｓ Ｉｎｃｏｒｐｏｒａｔｅｄ ヌクレオチド組成が限定されたプライマーを用いたデジタル増幅
KR20200047706A (ko) 2017-09-11 2020-05-07 트위스트 바이오사이언스 코포레이션 Gpcr 결합 단백질 및 이의 합성 방법
WO2019053215A1 (en) 2017-09-15 2019-03-21 F. Hoffmann-La Roche Ag HYBRIDIZATION-EXTENSION-LIGATURE STRATEGY TO GENERATE CIRCULAR SINGLE-STRAND DNA BANKS
US11680283B2 (en) 2017-09-22 2023-06-20 University Of Washington In situ combinatorial labeling of cellular molecules
CN111971124B (zh) * 2017-09-25 2022-08-23 普莱克斯姆公司 寡核苷酸编码的化学文库
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP3697529B1 (en) 2017-10-20 2023-05-24 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
US10267790B1 (en) * 2017-11-17 2019-04-23 Ultima Genomics, Inc. Systems for biological sample processing and analysis
EP3728636A1 (en) 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
WO2019136175A1 (en) 2018-01-04 2019-07-11 Twist Bioscience Corporation Dna-based digital information storage
US11584929B2 (en) 2018-01-12 2023-02-21 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
CN111801427B (zh) * 2018-02-05 2023-12-05 豪夫迈·罗氏有限公司 用于单分子的单链环状dna模板的产生
WO2019157034A1 (en) * 2018-02-07 2019-08-15 Nugen Technologies, Inc. Library preparation
US11807903B2 (en) * 2018-02-08 2023-11-07 Universal Sequencing Technology Corporation Methods and compositions for tracking nucleic acid fragment origin for nucleic acid sequencing
WO2019195701A1 (en) 2018-04-05 2019-10-10 Massachusetts Eye And Ear Infirmary Methods of making and using combinatorial barcoded nucleic acid libraries having defined variation
CN112243461B (zh) 2018-05-03 2024-07-12 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
KR20210016560A (ko) 2018-06-06 2021-02-16 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 핵산 라이브러리의 생성 방법 및 이를 실행하기 위한 조성물 및 키트
WO2020072380A1 (en) 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
CN109411011B (zh) * 2018-11-06 2022-05-17 苏州泓迅生物科技股份有限公司 一种引物组的设计方法及其应用
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
FI3899037T3 (fi) * 2018-12-19 2023-11-21 Illumina Inc Menetelmiä polynukleotidiklusterin klonaalisuusprioriteetin parantamiseksi
CN111379032A (zh) * 2018-12-28 2020-07-07 北京贝瑞和康生物技术有限公司 一种用于构建同时实现基因组拷贝数变异检测和基因突变检测的测序文库的方法和试剂盒
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
US11661631B2 (en) 2019-01-23 2023-05-30 Becton, Dickinson And Company Oligonucleotides associated with antibodies
JP2022519045A (ja) 2019-01-31 2022-03-18 ガーダント ヘルス， インコーポレイテッド 無細胞ｄｎａを単離するための組成物および方法
WO2020176678A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
KR20220004646A (ko) * 2019-04-05 2022-01-11 코넬 유니버시티 인공 대사를 가지는 동적 물질을 생성하기 위한 시스템 및 방법
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
AU2020280104A1 (en) 2019-05-22 2022-01-20 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
EP3980537A4 (en) * 2019-06-04 2023-11-22 Universal Sequencing Technology Corporation METHOD FOR BARCODING NUCLEIC ACIDS FOR DETECTION AND SEQUENCING
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
WO2021097252A1 (en) * 2019-11-13 2021-05-20 Bradley Bernstein Methylation assays and uses thereof
EP4073270A4 (en) * 2019-12-09 2024-01-10 Laboratorios Maymó S.A. RAPID AMPLIFICATION AND GENOTYPING OF NUCLEIC ACID SEQUENCES
GB2589869A (en) * 2019-12-09 2021-06-16 Univ Oxford Innovation Ltd Method for whole genome sequencing of picogram quantities of DNA
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
WO2021202970A1 (en) * 2020-04-02 2021-10-07 The Broad Institute, Inc. Sequencing-based population scale screening
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN113252892B (zh) * 2021-06-22 2021-11-02 北京市肝病研究所 一种用于提高病原微生物抗原检测灵敏度的探针及试剂盒
WO2023235179A1 (en) * 2022-05-30 2023-12-07 The Regents Of The University Of California Methods and compositions for generating spatially resolved genomic profiles from tissues

Family Cites Families (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0197196A1 (en) 1985-03-08 1986-10-15 The University Of Rochester Electro-electron optical oscilloscope system for time-resolving picosecond electrical waveforms
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
CA2206486A1 (en) * 1994-12-08 1996-06-13 Peter Alestrom Chemical labelling of objects
EP1736554B1 (en) 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
CN1195872C (zh) * 1996-06-06 2005-04-06 林克斯治疗公司 通过连接经编码的衔接子进行测序
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6521428B1 (en) 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
US6709816B1 (en) * 1999-10-18 2004-03-23 Affymetrix, Inc. Identification of alleles
AU2001234608A1 (en) 2000-01-28 2001-08-07 Genetrace Systems, Inc. Methods for analysis of gene expression
US20010031468A1 (en) 2000-02-08 2001-10-18 Alex Chenchik Analyte assays employing universal arrays
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
CA2419159A1 (en) 2000-08-11 2002-02-21 Agilix Corporation Ultra-sensitive detection systems
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
EP1334347A1 (en) 2000-09-15 2003-08-13 California Institute Of Technology Microfabricated crossflow devices and methods
US20040110191A1 (en) 2001-01-31 2004-06-10 Winkler Matthew M. Comparative analysis of nucleic acids using population tagging
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn AMPLIFICATION OF NUCLEIC ACID USING MICROFLUIDIC DEVICES
US20030170675A1 (en) 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
US7153656B2 (en) 2001-09-11 2006-12-26 Los Alamos National Security, Llc Nucleic acid sequence detection using multiplexed oligonucleotide PCR
WO2003033741A1 (en) 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
US20030119004A1 (en) 2001-12-05 2003-06-26 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
AU2003205817A1 (en) 2002-01-15 2003-07-30 Matforsk Methods of nucleic acid amplification
EP2666849A3 (en) 2002-04-01 2014-05-28 Fluidigm Corporation Microfluidic particle-analysis systems
CN102344960B (zh) 2002-09-06 2014-06-18 波士顿大学信托人 基因表达的定量
WO2004027082A2 (en) 2002-09-19 2004-04-01 Applera Corporation Methods and compositions for detecting targets
EP1551753A2 (en) 2002-09-25 2005-07-13 California Institute Of Technology Microfluidic large scale integration
EP1546412B1 (en) 2002-10-02 2014-05-21 California Institute Of Technology Microfluidic nucleic acid analysis
US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
EP3000899A1 (en) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplex amplification of polynucleotides
US20040110153A1 (en) 2002-12-10 2004-06-10 Affymetrix, Inc. Compleixity management of genomic DNA by semi-specific amplification
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
EP1590477B1 (en) * 2003-01-29 2009-07-29 454 Corporation Methods of amplifying and sequencing nucleic acids
US20040209299A1 (en) 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
DK1620568T3 (da) * 2003-04-24 2009-03-30 Afshin Ahmadian Analyse til allelspecifik mutationspåvisning
ATE431854T1 (de) * 2003-06-27 2009-06-15 Nanosphere Inc Auf bio-barcodes beruhender nachweis von zielanalyten
CN101001960A (zh) * 2003-06-27 2007-07-18 西北大学 基于生物条形码检测靶分析物
DE602004026077D1 (de) 2003-09-10 2010-04-29 Althea Technologies Inc Erstellung von expressionsprofilen unter verwendung von mikroarrays
KR100571817B1 (ko) 2003-09-19 2006-04-17 삼성전자주식회사 태그 서열에 혼성화하는 검출 프로브를 이용하는 표적핵산의 검출방법
NZ546903A (en) 2003-10-13 2009-06-26 Genaco Biomedical Products Inc Method and kit for primer based amplification of nucleic acids
EP1678327A4 (en) 2003-10-16 2007-10-10 Genomic Health Inc QRT-PCR TEST SYSTEM FOR GENE EXPRESSION PROFILING
US20090233272A1 (en) 2003-12-23 2009-09-17 Autogenomics, Inc. Multiplexed Nucleic Acid Analysis with High Specificity
DE602005014453D1 (de) 2004-03-24 2009-06-25 Applied Biosystems Llc Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
JP5502275B2 (ja) 2004-05-02 2014-05-28 フルイディグム コーポレイション 熱反応デバイスおよびその熱反応デバイスの使用方法
US20060053503A1 (en) 2004-07-30 2006-03-09 Ut-Battelle, Llc Cranial and vertebral defects associated with loss-of-function of Nell
US7709201B2 (en) 2004-08-24 2010-05-04 Cornell Research Foundation, Inc. Detection of nucleic acid differences using endonuclease cleavage/ligase resealing reactions and capillary electrophoresis or microarrays
EP1831401B1 (en) 2004-12-29 2010-02-10 Applied Biosystems, LLC Methods, compositions, and kits for forming self-complementary polynucleotides
WO2006112780A1 (en) * 2005-04-20 2006-10-26 Sedna Biotechnologies Ab Method for amplification
US20060263789A1 (en) * 2005-05-19 2006-11-23 Robert Kincaid Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using
CA2609328A1 (en) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
US20070077570A1 (en) 2005-05-31 2007-04-05 Applera Corporation Multiplexed amplification of short nucleic acids
EP2477029A1 (en) 2005-06-02 2012-07-18 Fluidigm Corporation Analysis using microfluidic partitioning devices
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
WO2007024798A2 (en) 2005-08-22 2007-03-01 Applera Corporation Apparatus, system, and method using immiscible-fluid-discrete-volumes
EP1938101A2 (en) 2005-09-13 2008-07-02 Fluidigm Corporation Microfluidic assay devices and methods
JP5237099B2 (ja) 2005-09-29 2013-07-17 キージーン ナムローゼ フェンノートシャップ 変異させた集団のハイスループットスクリーニング
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
EP1994179A2 (en) 2006-02-18 2008-11-26 Michael Strathmann Massively multiplexed sequencing
ES2301342B1 (es) * 2006-03-14 2009-05-01 Oryzon Genomics, S.A. "metodo de analisis de acidos nucleicos".
US8828661B2 (en) 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
WO2007147018A1 (en) * 2006-06-14 2007-12-21 Cellpoint Diagnostics, Inc. Analysis of rare cell-enriched samples
WO2007149789A2 (en) 2006-06-22 2007-12-27 Applera Corporation Conversion of target specific amplification to universal sequencing
CN1896284B (zh) * 2006-06-30 2013-09-11 博奥生物有限公司 一种鉴别等位基因类型的方法
WO2008015396A2 (en) * 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
WO2008061193A2 (en) 2006-11-15 2008-05-22 Biospherex Llc Multitag sequencing and ecogenomics analysis
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
US8157434B2 (en) 2007-01-19 2012-04-17 Fluidigm Corporation High efficiency and high precision microfluidic devices and methods
WO2008097841A2 (en) 2007-02-02 2008-08-14 California Institute Of Technology Surface chemistry and deposition techniques
EP2121983A2 (en) * 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
CN101067156A (zh) 2007-05-18 2007-11-07 中国人民解放军第三军医大学第一附属医院 一种基于选择性探针的多重pcr方法及其应用
GB0712882D0 (en) 2007-07-03 2007-08-15 Leicester University Of Nucleic acid amplification
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
US20090053719A1 (en) 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
US9440231B2 (en) 2007-08-14 2016-09-13 Fluidigm Corporation Polymer microfluidic biochip fabrication
WO2009036525A2 (en) 2007-09-21 2009-03-26 Katholieke Universiteit Leuven Tools and methods for genetic tests using next generation sequencing
US8268564B2 (en) * 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
US20090163366A1 (en) 2007-12-24 2009-06-25 Helicos Biosciences Corporation Two-primer sequencing for high-throughput expression analysis
WO2009102878A2 (en) 2008-02-12 2009-08-20 Nugen Technologies, Inc. Method for archiving and clonal expansion
WO2009105609A1 (en) 2008-02-22 2009-08-27 Fluidigm Corporation Integrated carrier for microfluidic device
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
US8309035B2 (en) * 2008-04-05 2012-11-13 Single Cell Technology, Inc. Multi-well system
US9068181B2 (en) 2008-05-23 2015-06-30 The General Hospital Corporation Microfluidic droplet encapsulation
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
US20100069250A1 (en) 2008-08-16 2010-03-18 The Board Of Trustees Of The Leland Stanford Junior University Digital PCR Calibration for High Throughput Sequencing
US8697363B2 (en) 2008-08-26 2014-04-15 Fluidigm Corporation Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags
US8748103B2 (en) * 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
SG172965A1 (en) 2009-01-13 2011-08-29 Fluidigm Corp Single-cell nucleic acid analysis
US8450063B2 (en) 2009-01-28 2013-05-28 Fluidigm Corporation Determination of copy number differences by amplification
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
ES2555389T3 (es) * 2009-03-30 2015-12-30 Illumina, Inc. Análisis de expresión génica en células individuales
SG10201402770YA (en) * 2009-04-02 2014-08-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
US20100285537A1 (en) 2009-04-02 2010-11-11 Fluidigm Corporation Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation
JP5663491B2 (ja) * 2009-10-29 2015-02-04 日本碍子株式会社 標的核酸の検出方法
WO2011091438A1 (en) 2010-01-25 2011-07-28 George Mason Intellectual Properties, Inc. Improved one-step cell and tissue preservative for morphologic and molecular analysis
EP2534247B1 (en) * 2010-02-12 2015-10-28 Genisphere, LLC Whole transcriptome sequencing
SG185543A1 (en) 2010-05-14 2012-12-28 Fluidigm Corp Assays for the detection of genotype, mutations, and/or aneuploidy
WO2011143659A2 (en) 2010-05-14 2011-11-17 Fluidigm Corporation Nucleic acid isolation methods
US20120034603A1 (en) * 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
EP2601307A4 (en) * 2010-08-06 2014-01-01 Capitalbio Corp MICROARRAY TEST WITH PARTICLES FOR THE ANALYSIS OF MOLECULAR INTERACTIONS
GB2497912B (en) * 2010-10-08 2014-06-04 Harvard College High-throughput single cell barcoding
MX340258B (es) * 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
AU2012209329A1 (en) 2011-01-24 2013-09-12 Epic Sciences, Inc. Methods for obtaining single cells and applications of single cell omics
US9150852B2 (en) * 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
KR101322880B1 (ko) * 2011-03-04 2013-10-29 한국과학기술원 Ｓｄｌ－ｐｃｒ을 이용한 유전자 분석방법
WO2012129363A2 (en) * 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
US20120316075A1 (en) * 2011-03-30 2012-12-13 Noblegen Biosciences, Inc. Sequence preserved dna conversion for optical nanopore sequencing
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
EP2710144B1 (en) * 2011-05-19 2020-10-07 Agena Bioscience, Inc. Processes for multiplex nucleic acid identification
CN103890245B (zh) 2011-05-20 2020-11-17 富鲁达公司 核酸编码反应
US9617598B2 (en) 2011-05-27 2017-04-11 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
US9586987B2 (en) 2011-09-08 2017-03-07 Kabushiki Kaisha Dnaform Primer set for isothermal amplication of a target nucleic acid sequence
GB2504240B (en) * 2012-02-27 2015-05-27 Cellular Res Inc Compositions and kits for molecular counting of nucleic acids
CN104471077B (zh) 2012-05-21 2017-05-24 富鲁达公司 颗粒群的单颗粒分析
CA2876209A1 (en) 2012-06-15 2013-12-19 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
WO2013188872A1 (en) * 2012-06-15 2013-12-19 The Board Of Regents Of The University Of Texas System High throughput sequencing of multiple transcripts of a single cell
WO2014008447A1 (en) * 2012-07-03 2014-01-09 Integrated Dna Technologies, Inc. Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
WO2014144495A1 (en) * 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
WO2014145128A2 (en) 2013-03-15 2014-09-18 Arnold Lyle J Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
JP6545682B2 (ja) * 2013-08-28 2019-07-17 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 大規模並列単一細胞分析
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
JP6672310B2 (ja) 2014-09-15 2020-03-25 アブビトロ， エルエルシー ハイスループットヌクレオチドライブラリーシークエンシング
WO2016100977A1 (en) 2014-12-19 2016-06-23 The Broad Institute Inc. Methods for profiling the t-cel- receptor repertoire
WO2016138490A1 (en) 2015-02-27 2016-09-01 Fluidigm Corporation Single-cell nucleic acids for high-throughput studies
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
EP3485043A4 (en) 2016-07-14 2020-03-25 Fluidigm Corporation SEQUENCING OF SINGLE-CELL TRANSCRIPTION PRODUCTS

Also Published As

Publication number Publication date
US9074204B2 (en) 2015-07-07
EP2710172A4 (en) 2015-06-17
WO2012162267A2 (en) 2012-11-29
US12018323B2 (en) 2024-06-25
US20220119874A1 (en) 2022-04-21
US20130005585A1 (en) 2013-01-03
SG194745A1 (en) 2013-12-30
US20160208322A1 (en) 2016-07-21
EP2710172B1 (en) 2017-03-29
CN112592960A (zh) 2021-04-02
WO2012162267A3 (en) 2014-05-15
CN103890245A (zh) 2014-06-25
CN103890245B (zh) 2020-11-17
EP2710172A2 (en) 2014-03-26
SG10201605049QA (en) 2016-07-28
US10501786B2 (en) 2019-12-10

Similar Documents

Publication Publication Date Title
HK1198661A1 (zh) 核酸編碼反應
EP2729182A4 (en) NUCLEIC COMPLEX
IL231983A0 (en) Dna containing tricyclo-phosphorothioate
EP2799548A4 (en) ANTISENSE NUCLEIC ACID
EP2917372A4 (en) NUCLEIC ACID SEQUENCING USING LABELS
LT3623481T (lt) Kompozicijos, skirtos nukleorūgščių sekoskaitai
EP2612917A4 (en) ANTISENSE NUCLEIC ACID
ZA201205891B (en) Dna polymerases
GB2494239B (en) Nucleic acid amplification
HK1203400A1 (zh) 提供過水解酶催化反應的系統
EP2764112A4 (en) DIRECT NUCLEIC ACID ANALYSIS
GB201204407D0 (en) Nucleic acid molecule
GB201118636D0 (en) Nucleotide sequence
GB2503856B (en) Methods for nucleic acid purification
EP2915815A4 (en) NEW MODIFIED NUCLEIC ACID
EP2798092A4 (en) nucleic acid hybridization probes
ZA201206793B (en) Nucleic acid complexes
LT2993232T (lt) Nukleorūgščių gavimo būdas
EP2700414A4 (en) SELF-GELATINABLE NUCLEIC ACID
EP2771462A4 (en) PURIFICATION OF A NUCLEIC ACID
EP2682462A4 (en) (Nucleic acid) polysaccharide COMPLEX
HK1193407A1 (zh) 吡咯烷- -基乙酸衍生物
EP2594592A4 (en) COMPLEX (NUCLEIC ACID) -POLYSACCHARIDE
GB201107286D0 (en) Nucleic acid preparation
EP2811020A4 (en) METHOD FOR ENHANCING A NUCLEIC ACID SYNTHESIS REACTION