HK1154627A1 - 位點取代或缺失的能增加熱穩定性的紅褐肉座菌 纖維素酶的新的變異體 - Google Patents

位點取代或缺失的能增加熱穩定性的紅褐肉座菌 纖維素酶的新的變異體

Info

Publication number
HK1154627A1
HK1154627A1 HK11108796.6A HK11108796A HK1154627A1 HK 1154627 A1 HK1154627 A1 HK 1154627A1 HK 11108796 A HK11108796 A HK 11108796A HK 1154627 A1 HK1154627 A1 HK 1154627A1
Authority
HK
Hong Kong
Prior art keywords
cbh1
deletion
substitution
thermal stability
cellulases
Prior art date
Application number
HK11108796.6A
Other languages
English (en)
Inventor
Anthony Day
Frits Goedegebuur
Peter Gualfetti
Colin Mitchinson
Paulien Neefe
Mats Sandgren
Andrew Shaw
Jerry Stahlberg
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of HK1154627A1 publication Critical patent/HK1154627A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Paper (AREA)
HK11108796.6A 2002-08-16 2011-08-20 位點取代或缺失的能增加熱穩定性的紅褐肉座菌 纖維素酶的新的變異體 HK1154627A1 (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40406302P 2002-08-16 2002-08-16
US45636803P 2003-03-21 2003-03-21
US45885303P 2003-03-27 2003-03-27
US45869603P 2003-03-27 2003-03-27

Publications (1)

Publication Number Publication Date
HK1154627A1 true HK1154627A1 (zh) 2012-04-27

Family

ID=31892262

Family Applications (5)

Application Number Title Priority Date Filing Date
HK08103624.0A HK1113497A1 (en) 2002-08-16 2008-04-02 Novel variant hyprocrea jecorina cbh1 cellulases
HK11108795.7A HK1154626A1 (zh) 2002-08-16 2011-08-20 紅褐肉座菌 纖維素酶的新的變異體
HK11108796.6A HK1154627A1 (zh) 2002-08-16 2011-08-20 位點取代或缺失的能增加熱穩定性的紅褐肉座菌 纖維素酶的新的變異體
HK11109033.7A HK1154889A1 (zh) 2002-08-16 2011-08-26 位點取代或缺失的能增加熱穩定性的紅褐肉座菌 纖維素酶的新的變異體
HK11109219.3A HK1154902A1 (zh) 2002-08-16 2011-08-31 位點取代或缺失的能增加熱穩定性的紅褐肉座菌 纖維素酶的新的變異體

Family Applications Before (2)

Application Number Title Priority Date Filing Date
HK08103624.0A HK1113497A1 (en) 2002-08-16 2008-04-02 Novel variant hyprocrea jecorina cbh1 cellulases
HK11108795.7A HK1154626A1 (zh) 2002-08-16 2011-08-20 紅褐肉座菌 纖維素酶的新的變異體

Family Applications After (2)

Application Number Title Priority Date Filing Date
HK11109033.7A HK1154889A1 (zh) 2002-08-16 2011-08-26 位點取代或缺失的能增加熱穩定性的紅褐肉座菌 纖維素酶的新的變異體
HK11109219.3A HK1154902A1 (zh) 2002-08-16 2011-08-31 位點取代或缺失的能增加熱穩定性的紅褐肉座菌 纖維素酶的新的變異體

Country Status (11)

Country Link
US (6) US7972832B2 (zh)
EP (6) EP2295559B1 (zh)
JP (1) JP4756861B2 (zh)
CN (2) CN101048500B (zh)
AT (1) ATE527344T1 (zh)
AU (1) AU2003263842A1 (zh)
CA (2) CA2865180A1 (zh)
DK (6) DK1578943T3 (zh)
ES (1) ES2375054T3 (zh)
HK (5) HK1113497A1 (zh)
WO (1) WO2004016760A2 (zh)

Families Citing this family (171)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1578943T3 (da) * 2002-08-16 2012-01-09 Danisco Us Inc Nye variant-Hypocrea jecorina-CBH1-cellulaser
WO2005028636A2 (en) 2003-03-21 2005-03-31 Genencor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
SI1627050T1 (sl) 2003-04-01 2014-01-31 Danisco Us Inc. Varianta humicola grisea cbh1.1
US7960148B2 (en) 2003-07-02 2011-06-14 Verenium Corporation Glucanases, nucleic acids encoding them and methods for making and using them
DK2617826T3 (en) 2003-08-25 2015-10-12 Novozymes Inc Variants of glucosidhydrolase
CN1989254B (zh) * 2004-07-27 2013-07-10 旭化成化学株式会社 生产纤维寡糖的方法
JP5343212B2 (ja) 2005-03-15 2013-11-13 ヴェレニウム コーポレイション セルラーゼ、それらをコードする核酸、並びにそれらを作製及び使用する方法
US7741093B2 (en) * 2005-04-29 2010-06-22 Ab Enzymes Oy Cellulases and their uses
FI120045B (fi) * 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
MY160710A (en) 2006-02-10 2017-03-15 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
CN101528766A (zh) 2006-08-04 2009-09-09 维莱尼姆公司 葡聚糖酶、编码它们的核酸及制备和使用它们的方法
US8017373B2 (en) * 2006-08-31 2011-09-13 Iogen Energy Corporation Process for enzymatic hydrolysis of pretreated lignocellulosic feedstocks
CA2670102A1 (en) * 2006-11-22 2008-05-29 The Trustees Of Dartmouth College Recombinant yeast strains expressing tethered cellulase enzymes
NZ598285A (en) * 2007-01-30 2013-10-25 Syngenta Participations Ag Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
BRPI0812267A2 (pt) * 2007-05-31 2014-10-14 Novozymes Inc Célula hospedeira fúngica filamentosa, método para produzir uma composição de proteína celulolítica, composição de proteína celulolítica, e, métodos para degradar ou converter um material, contendo celulose e para produzir um produto de fermentação
EA018049B1 (ru) * 2007-06-08 2013-05-30 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН Система экспрессии гетерологичных и гомологичных целлюлаз
MX2010002180A (es) * 2007-08-30 2010-06-01 Iogen Energy Corp Hidrolisis enzimatica de materiales de abastecimiento lignocelulosicos utilizando enzimas auxiliares.
AU2008343105A1 (en) 2007-12-19 2009-07-09 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
DK2288698T3 (en) * 2008-06-06 2017-03-20 Danisco Us Inc COMPOSITIONS AND PROCEDURES INCLUDING CELLULASE-VARIABLE VARIETIES FOR CELLULOSE-FREE MATERIALS
EP2318523A4 (en) * 2008-08-01 2011-11-23 Iogen Energy Corp CELLULASE OF FAMILY 6 WITH INACTIVATION BY LOWERED LIGNIN
CN102300986A (zh) 2008-12-04 2011-12-28 诺维信股份有限公司 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
US8426158B2 (en) 2008-12-19 2013-04-23 Novozymes, Inc. Methods for increasing enzymatic hydrolysis of cellulosic material in the presence of a peroxidase
CN102325889A (zh) 2008-12-19 2012-01-18 诺维信股份有限公司 增加纤维素材料水解的方法
US8338121B2 (en) 2008-12-19 2012-12-25 Novozymes, Inc. Methods for determining cellulolytic enhancing activity of a polypeptide
DK2379712T3 (en) 2008-12-19 2017-05-08 Novozymes Inc Methods for Increasing Hydrolysis of Cellulosic Material in the Presence of Cellobiose Dehydrogenase
CN102388134A (zh) 2009-01-28 2012-03-21 诺维信股份有限公司 具有β-葡糖苷酶活性的多肽和编码该多肽的多核苷酸
US8629324B2 (en) 2009-01-30 2014-01-14 Novozymes, Inc. Polypeptides having expansin activity and polynucleotides encoding same
US9279112B2 (en) 2009-02-27 2016-03-08 Iogen Energy Corporation Cellulase enzymes having a modified linker and reduced lignin binding
WO2010107303A2 (en) 2009-03-16 2010-09-23 Dyadic Nederland B.V. New fungal production system
EP2408908B1 (en) * 2009-03-17 2015-05-27 Codexis, Inc. Variant endoglucanases and related polynucleotides
WO2010108918A1 (en) 2009-03-24 2010-09-30 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
DK2435561T3 (en) 2009-05-29 2018-11-05 Novozymes Inc PROCEDURES FOR IMPROVING THE DEGRADATION OR CONVERSION OF CELLULOSE SUBSTANCES
US8278507B2 (en) 2009-06-02 2012-10-02 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
BR112012000260B1 (pt) 2009-07-07 2020-11-17 Novozymes A/S célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo, para produzir um mutante de uma célula precursora, para inibir a expressão de um polipeptídeo, para produzir uma proteína, para degradar ou converter um material celulósico, para produzir um produto de fermentação, para fermentar um material celulósico, construções de ácido nucleico, vetor de expressão, polipeptídeo isolado tendo atividade de intensificação celulolítica, e, polinucleotídeo isolado que codifica o mesmo
EP2478096B1 (en) 2009-09-17 2017-05-24 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2478095A1 (en) 2009-09-18 2012-07-25 Novozymes Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
EP3296394B1 (en) 2009-09-23 2020-11-04 Danisco US Inc. Novel glycosyl hydrolase enzymes and uses thereof
CN107338233A (zh) 2009-09-29 2017-11-10 诺维信股份有限公司 具有木聚糖酶活性的多肽和编码该多肽的多核苷酸
WO2011041397A1 (en) 2009-09-29 2011-04-07 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN102695720B (zh) 2009-09-30 2018-02-16 诺维信股份有限公司 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
US8586829B2 (en) 2009-09-30 2013-11-19 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2491122A1 (en) 2009-10-23 2012-08-29 Novozymes Inc. Cellobiohydrolase variants and polynucleotides encoding same
WO2011059740A1 (en) 2009-10-29 2011-05-19 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
ES2574054T3 (es) 2009-11-06 2016-06-14 Novozymes, Inc. Polipéptidos con actividad de xilanasa y polinucleótidos que los codifican
DK2496693T3 (en) 2009-11-06 2018-01-22 Novozymes Inc Polypeptides with cellobiohydrolase activity and polynucleotides encoding them
WO2011079048A2 (en) 2009-12-23 2011-06-30 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
WO2011097713A1 (en) 2010-02-11 2011-08-18 Iogen Energy Corporation Carbohydrate binding modules with reduced binding to lignin
DK2357227T3 (en) * 2010-02-11 2015-09-14 Süd Chemie Ip Gmbh & Co Kg Optimized cellulase enzymes
CN102918151B (zh) 2010-03-31 2015-09-09 诺维信股份有限公司 纤维二糖水解酶变体及编码其的多核苷酸
WO2012003379A1 (en) 2010-06-30 2012-01-05 Novozymes A/S Polypeptides having beta-glucosidase activity and polynucleotides encoding same
WO2012021408A1 (en) 2010-08-12 2012-02-16 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and a dioxy compound and uses thereof
WO2012030858A2 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having hemicellulolytic activity and polynucleotides encoding same
EP2611901B1 (en) 2010-08-30 2016-05-11 Novozymes A/S Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
EP2611914A1 (en) 2010-08-30 2013-07-10 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012030811A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US8624082B2 (en) 2010-08-30 2014-01-07 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
US9267126B2 (en) 2010-08-30 2016-02-23 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2616542A4 (en) * 2010-09-15 2014-01-22 Univ California THERMOPHILIC MUTANTS OF ENDOGLUCANASE I FROM TRICHODERMA REESEI
CN103282489B (zh) 2010-09-30 2016-12-14 诺维信股份有限公司 具有纤维素分解增强活性的多肽变体及其编码多核苷酸
WO2012044836A1 (en) 2010-09-30 2012-04-05 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012044915A2 (en) 2010-10-01 2012-04-05 Novozymes, Inc. Beta-glucosidase variants and polynucleotides encoding same
CN103562402A (zh) * 2010-10-20 2014-02-05 丹尼斯科美国公司 热稳定性木霉属纤维素酶
WO2012058293A1 (en) 2010-10-26 2012-05-03 Novozymes North America, Inc. Methods of saccharifying sugarcane trash
WO2012061517A1 (en) 2010-11-02 2012-05-10 Novozymes, Inc. Methods of pretreating cellulosic material with a gh61 polypeptide
EP2635594B1 (en) 2010-11-04 2017-01-11 Novozymes Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP2638153B1 (en) 2010-11-12 2017-07-12 Novozymes Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
BR112013010129A2 (pt) 2010-11-18 2016-07-05 Novozymes Inc polipeptídeo gh61 quimérico isolado, polinucleotídeo isolado, célula hospedeira, métodos para produzir um polipeptídeo gh61 quimérico, para degradas ou converter um material celulóstico, para sintetizar um produto de fermentação, para fermentar um material celulósico e para limpar ou lavar uma superfície dura ou roupa para lavar, planta transgênica, parte da planta ou célula de planta, uso do polipeptídeo gh61 quimérico, composição detergente, e, formulação de caldo ou composição de cultura de células completas
WO2012078656A1 (en) 2010-12-06 2012-06-14 Novozymes North America, Inc. Methods of hydrolyzing oligomers in hemicellulosic liquor
CN110628803A (zh) 2011-01-26 2019-12-31 诺维信公司 具有纤维二糖水解酶活性的多肽及编码该多肽的多核苷酸
DK2668268T3 (en) 2011-01-26 2017-09-11 Novozymes Inc POLYPEPTIDES WITH CELLOBIO HYDROLASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
WO2012103322A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
MX2013007720A (es) 2011-01-26 2013-08-09 Novozymes As Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican para los mismos.
MX337919B (es) 2011-01-26 2016-03-28 Novozymes As Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos.
US8790894B2 (en) * 2011-01-31 2014-07-29 Dsm Ip Assets B.V. Mutant cellobiohydrolase
US9434972B2 (en) 2011-01-31 2016-09-06 Novozymes North America, Inc. Processes for enzymatic refining of pretreated cellulosic material for saccharification
DK2678352T3 (en) 2011-02-23 2018-03-05 Novozymes Inc Polypeptides with cellulolysis enhancing activity and polynucleotides encoding them
WO2012122518A1 (en) 2011-03-09 2012-09-13 Novozymes A/S Methods of increasing the cellulolytic enhancing activity of a polypeptide
US9409958B2 (en) 2011-03-10 2016-08-09 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
BR112013023757A2 (pt) 2011-03-17 2017-06-06 Danisco Us Inc método para redução de viscosidade no processo de sacarificação
EP3333258A3 (en) 2011-03-25 2018-07-25 Novozymes A/S Method for degrading or converting cellulosic material
EP2691519A1 (en) 2011-03-31 2014-02-05 Novozymes, Inc. Cellulose binding domain variants and polynucleotides encoding same
WO2012135659A2 (en) 2011-03-31 2012-10-04 Novozymes A/S Methods for enhancing the degradation or conversion of cellulosic material
CN103649308A (zh) 2011-04-28 2014-03-19 诺维信股份有限公司 具有内切葡聚糖酶活性的多肽和编码该多肽的多核苷酸
CN103797126A (zh) 2011-04-29 2014-05-14 诺维信股份有限公司 用于增强纤维素材料的降解或转化的方法
WO2012159009A1 (en) 2011-05-19 2012-11-22 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
EP2710132A1 (en) 2011-05-19 2014-03-26 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
CN109022518A (zh) 2011-07-22 2018-12-18 诺维信北美公司 用于预处理纤维素材料和改进其水解的方法
EP2739727B1 (en) 2011-08-04 2016-05-25 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
CA2838758A1 (en) 2011-08-04 2013-02-07 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
BR112014004190A2 (pt) 2011-08-24 2017-03-01 Novozymes Inc método para construir uma cepa fúngida filamentosa, cepa fúngida filamentosa, método para produzir múltiplos polipeptídeos recombinantes, e, construto em tandem
EP2748321A2 (en) 2011-08-24 2014-07-02 Novozymes, Inc. Methods for obtaining positive transformants of a filamentous fungal host cell
US9279163B2 (en) 2011-08-31 2016-03-08 Iogen Energy Corporation Cellobiohydrolase enzymes
EP2756091A1 (en) 2011-09-13 2014-07-23 Novozymes North America, Inc. Methods of hydrolyzing and fermenting cellulosic material
WO2013043910A1 (en) 2011-09-20 2013-03-28 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN103930438A (zh) 2011-09-30 2014-07-16 诺维信股份有限公司 具有β-葡糖苷酶活性的嵌合多肽和对其进行编码的多核苷酸
AR088257A1 (es) * 2011-10-06 2014-05-21 Bp Corp North America Inc Polipeptidos de cbh (celobiohidrolasas) i variantes con reducida inhibicion de producto
EP2773656B1 (en) 2011-10-31 2019-06-19 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2780449B1 (en) 2011-11-18 2018-04-11 Novozymes, Inc. Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
CN109112118A (zh) 2011-11-21 2019-01-01 诺维信股份有限公司 Gh61多肽变体以及编码所述变体的多核苷酸
BR112014011387A2 (pt) 2011-11-22 2017-05-02 Novozymes Inc polipeptídeo e polinucleotídeo isolados, célula hospedeira recombinante, métodos para produzir um polipeptídeo, um mutante de uma célula precursora, e uma proteína, e para inibir a expressão de um polipeptídeo, planta transgênica, parte da planta ou célula de planta, molécula de rna, processos para degradar ou converter um material celulósico ou contendo xilano, para sintetizar um produto de fermentação, e para fermentar um material celulósico ou contendo xilano, e, formulação de caldo completo ou composição de cultura de célula
US9624481B2 (en) 2011-12-01 2017-04-18 Novozymes, Inc. Polypeptides having beta-xylosidase activity and polynucleotides encoding same
EP2791330B1 (en) 2011-12-16 2017-07-26 Novozymes, Inc. Polypeptides having laccase activity and polynucleotides encoding same
WO2013096369A1 (en) 2011-12-19 2013-06-27 Novozymes A/S Processes and compositions for increasing the digestibility of cellulosic materials
BR112014014697A2 (pt) 2011-12-19 2020-10-27 Novozymes, Inc. polipeptídeo isolado, composição, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, métodos para produzir um polipeptídeo e uma proteína, para gerar oxigênio molecular, e para remover peróxido de hidrogênio do tecido, processos para degradar ou converter um material celulósico, e para produzir um produto de fermentação, e, formulação de caldo integral ou composição de cultura de células
WO2013096603A2 (en) 2011-12-20 2013-06-27 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
US20150017670A1 (en) 2011-12-21 2015-01-15 Novozymes, Inc. Methods For Determining The Degradation Of A Biomass Material
CN104245929A (zh) 2012-04-23 2014-12-24 诺维信公司 具有α-葡糖醛酸糖苷酶活性的多肽以及编码其的多核苷酸
DK2841570T3 (en) 2012-04-23 2018-03-12 Novozymes As POLYPEPTIDES WITH GLUCURONYL STERASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
BR112014026625A2 (pt) 2012-04-27 2017-07-18 Novozymes Inc E Novozymes As variante do polipeptídeo, polinucleotídeo isolado, célula hospedeira recombinante, métodos para produção de uma variante do polipeptídeo e para obtenção de uma variante do polipeptídeo, planta, parte de planta ou célula de planta transgênica, processos para degradação ou conversão de um material celulósico, para produção de um produto da fermentação e para fermentação de um material celulósico, composição, e, formulação ou composição de cultura celular de caldo inteiro
WO2014092832A2 (en) 2012-09-19 2014-06-19 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
EP3586610B1 (en) 2012-10-08 2024-09-25 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US20150275194A1 (en) 2012-10-24 2015-10-01 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
US20150315560A1 (en) * 2012-12-12 2015-11-05 Danisco Us Inc. Variants of cellobiohydrolases
EP2931886A1 (en) * 2012-12-12 2015-10-21 Danisco US Inc. Variants of cellobiohydrolases
BR112015013759A2 (pt) * 2012-12-12 2017-11-14 Danisco Us Inc variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo de variante de cbh e sobrenadante de cultura celular
CA2892786A1 (en) * 2012-12-12 2014-06-19 Danisco Us Inc. Variants of cellobiohydrolases
KR20150095726A (ko) * 2012-12-12 2015-08-21 다니스코 유에스 인크. 셀로바이오하이드롤라제의 변이체
CN104870639A (zh) 2012-12-14 2015-08-26 诺维信公司 具有纤维素分解增强活性的多肽以及编码它们的多核苷酸
WO2014099798A1 (en) 2012-12-19 2014-06-26 Novozymes A/S Polypeptides having cellulolytic enhancinc activity and polynucleotides encoding same
WO2014138672A1 (en) 2013-03-08 2014-09-12 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
WO2014155566A1 (ja) 2013-03-27 2014-10-02 本田技研工業株式会社 耐熱性セロビオハイドロラーゼ
BR112016002067A2 (pt) 2013-07-29 2017-08-29 Danisco Us Inc Variantes de enzimas
JP6357702B2 (ja) 2014-03-13 2018-07-18 本田技研工業株式会社 耐熱性セロビオハイドロラーゼ及びそのアミノ酸置換変異体
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
JP6441330B2 (ja) * 2014-05-27 2018-12-19 国立研究開発法人産業技術総合研究所 熱安定性が改善されたセロビオハイドロラーゼ
FR3022557B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
CN107002056A (zh) 2014-09-05 2017-08-01 诺维信公司 碳水化合物结合模块变体以及编码它们的多核苷酸
WO2016040265A1 (en) 2014-09-08 2016-03-17 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
JP6429377B2 (ja) * 2014-12-12 2018-11-28 本田技研工業株式会社 耐熱性セロビオハイドロラーゼ
WO2016120298A1 (en) 2015-01-28 2016-08-04 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
PL3250698T3 (pl) 2015-01-28 2020-06-29 Dsm Ip Assets B.V. Sposób enzymatycznej hydrolizy materiału lignocelulozowego i fermentacji cukrów
CA2973303C (en) 2015-01-28 2023-01-03 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP3262165B1 (en) 2015-02-24 2020-06-03 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
EP3067428A1 (en) 2015-03-12 2016-09-14 BETA RENEWABLES S.p.A. A process for producing a hydrolyzed mixture from a pre-treated ligno-cellulosic slurry comprising a slurry liquid and slurry solids
WO2016169892A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016169893A1 (en) 2015-04-20 2016-10-27 Dsm Ip Assets B.V. Whole fermentation broth
WO2016207144A1 (en) 2015-06-22 2016-12-29 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP3320106A1 (en) 2015-07-07 2018-05-16 Danisco US Inc. Induction of gene expression using a high concentration sugar mixture
EP4410974A2 (en) 2016-03-02 2024-08-07 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
DK3433358T3 (da) * 2016-03-24 2022-09-26 Novozymes As Cellobiohydrolasevarianter og polynukleotider, der koder for dem
WO2017211957A1 (en) 2016-06-09 2017-12-14 Dsm Ip Assets B.V. Seed train for large scale enzyme production
CN107540689B (zh) * 2016-06-23 2019-09-17 南方科技大学 抗肿瘤药物的合成方法
CN107540688B (zh) * 2016-06-23 2019-09-17 南方科技大学 抗肿瘤药物的合成方法
US10913938B2 (en) 2016-07-29 2021-02-09 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
WO2018053058A1 (en) 2016-09-14 2018-03-22 Danisco Us Inc. Lignocellulosic biomass fermentation-based processes
EP3523415A1 (en) 2016-10-04 2019-08-14 Danisco US Inc. Protein production in filamentous fungal cells in the absence of inducing substrates
EP3545100A1 (en) 2016-11-24 2019-10-02 DSM IP Assets B.V. Enzyme composition
US20190276809A1 (en) 2016-11-24 2019-09-12 Dsm Ip Assets B.V. Enzyme composition
EP3558026A1 (en) 2016-12-21 2019-10-30 DuPont Nutrition Biosciences ApS Methods of using thermostable serine proteases
WO2018185071A1 (en) 2017-04-03 2018-10-11 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2019074828A1 (en) 2017-10-09 2019-04-18 Danisco Us Inc CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE
FI3695001T3 (fi) 2017-10-09 2024-02-14 Versalis Spa Menetelmä lignoselluloosamateriaalin entsymaattista hydrolyysiä ja sokerien fermentointia varten
WO2019086369A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2019086370A1 (en) 2017-10-30 2019-05-09 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
EP3775189A1 (en) 2018-03-28 2021-02-17 DSM IP Assets B.V. Enzyme composition
WO2019185681A1 (en) 2018-03-28 2019-10-03 Dsm Ip Assets B.V. Enzyme composition
BR112020023198A2 (pt) 2018-05-17 2021-02-09 Dsm Ip Assets B.V. processo para produção de um polipeptídeo
WO2019229108A1 (en) 2018-05-30 2019-12-05 Dsm Ip Assets B.V. Process for producing sugars from carbohydrate materials
CN109234277B (zh) * 2018-09-06 2021-05-25 中国农业科学院北京畜牧兽医研究所 转录效率提高的cbh1定向改造启动子Su-m3及其应用
WO2020058249A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058253A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
WO2020058248A1 (en) 2018-09-18 2020-03-26 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of carbohydrate material and fermentation of sugars
CN112912511A (zh) 2018-10-24 2021-06-04 帝斯曼知识产权资产管理有限公司 用于碳水化合物材料酶促水解和糖发酵的方法
EP3938525A1 (en) 2019-03-12 2022-01-19 DSM IP Assets B.V. Process for producing a fermentation broth
EP4028536A1 (en) 2019-09-10 2022-07-20 DSM IP Assets B.V. Enzyme composition
WO2022013148A1 (en) 2020-07-13 2022-01-20 Dsm Ip Assets B.V. Process for the production of biogas
EP4320252A1 (en) 2021-04-06 2024-02-14 DSM IP Assets B.V. Enzyme composition
CA3213845A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
WO2022214459A1 (en) 2021-04-06 2022-10-13 Dsm Ip Assets B.V. Enzyme composition
BR112023014100A2 (pt) 2021-04-08 2023-10-17 Versalis Spa Processo para a preparação de um produto de açúcar e um produto de fermentação

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1368599A (en) 1970-09-29 1974-10-02 Unilever Ltd Softening compositions
GB1358599A (en) 1970-12-22 1974-07-03 Monacelli Umberto Gun for firing tacks
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
GB2095275B (en) 1981-03-05 1985-08-07 Kao Corp Enzyme detergent composition
GB2094826B (en) 1981-03-05 1985-06-12 Kao Corp Cellulase enzyme detergent composition
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4822516A (en) 1986-12-08 1989-04-18 Kao Corporation Detergent composition for clothing incorporating a cellulase
WO1989009259A1 (en) * 1988-03-24 1989-10-05 Novo-Nordisk A/S A cellulase preparation
US5776757A (en) 1988-03-24 1998-07-07 Novo Nordisk A/S Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
DK494089D0 (zh) 1989-10-06 1989-10-06 Novo Nordisk As
US5650322A (en) 1990-10-05 1997-07-22 Genencor International, Inc. Methods for stonewashing fabrics using endoglucanases
US5246853A (en) 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
DK0551386T3 (da) 1990-10-05 2004-05-10 Genencor Int Fremgangsmåde til behandling af bomuldsholdige stoffer med cellulase
US5475101A (en) 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
CA2093422C (en) * 1990-10-05 2001-04-03 DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS
CN1065915C (zh) * 1991-03-18 2001-05-16 佛罗里达大学 通过重组宿主生产乙醇
FI932521A0 (fi) 1993-06-02 1993-06-02 Alko Ab Oy Nytt endoglukanasenzym
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
GB9400623D0 (en) * 1994-01-14 1994-03-09 Univ Leeds Exploitation of the cellulase enzyme complex of neurospora
ATE452979T1 (de) 1996-11-13 2010-01-15 Du Pont Herstellungsverfahren von 1,3-propandiol durch rekombinante organismen
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
DE69822142T2 (de) * 1997-04-07 2005-03-03 Unilever N.V. Transformation von schimmeln durch agrobacterium, insbesondere von der gattung aspergillus
WO1999006574A1 (en) * 1997-07-31 1999-02-11 Dsm N.V. Cellulose degrading enzymes of aspergillus
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
US6599722B2 (en) * 1998-12-22 2003-07-29 Genencor International, Inc. Method for producing ascorbic acid intermediates
WO2000039288A1 (fr) * 1998-12-24 2000-07-06 Takara Shuzo Co., Ltd. Polypeptides
WO2001004284A1 (en) * 1999-07-13 2001-01-18 Midwest Research Institute Cellobiohydrolase reduced glycosylation variants: cbhin45a; cbhin270a; and cbhin384a
US7375197B2 (en) * 2002-01-14 2008-05-20 Midwest Research Institute Cellobiohydrolase I gene and improved variants
DK1578943T3 (da) 2002-08-16 2012-01-09 Danisco Us Inc Nye variant-Hypocrea jecorina-CBH1-cellulaser
DK2617826T3 (en) * 2003-08-25 2015-10-12 Novozymes Inc Variants of glucosidhydrolase

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