HK1127066A1 - Droplet-based biochemistry - Google Patents

Droplet-based biochemistry

Info

Publication number
HK1127066A1
HK1127066A1 HK09105694.9A HK09105694A HK1127066A1 HK 1127066 A1 HK1127066 A1 HK 1127066A1 HK 09105694 A HK09105694 A HK 09105694A HK 1127066 A1 HK1127066 A1 HK 1127066A1
Authority
HK
Hong Kong
Prior art keywords
protocols
droplet microactuator
droplet
reagents
based biochemistry
Prior art date
Application number
HK09105694.9A
Other languages
English (en)
Inventor
Michael G Pollack
Vamsee K Pamula
Vijay Srinivasan
Philip Y Paik
Allen E Eckhardt
Richard B Fair
Original Assignee
Advanced Liquid Logic Inc
Univ Duke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Liquid Logic Inc, Univ Duke filed Critical Advanced Liquid Logic Inc
Publication of HK1127066A1 publication Critical patent/HK1127066A1/xx

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3031—Micromixers using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0436—Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
    • B01L2400/0448—Marangoni flow; Thermocapillary effect
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Colloid Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
HK09105694.9A 2006-04-18 2009-06-24 Droplet-based biochemistry HK1127066A1 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US74505806P 2006-04-18 2006-04-18
US74503906P 2006-04-18 2006-04-18
US74505906P 2006-04-18 2006-04-18
US74507306P 2006-04-18 2006-04-18
US74595006P 2006-04-28 2006-04-28
US74591406P 2006-04-28 2006-04-28
US74680106P 2006-05-09 2006-05-09
US74679706P 2006-05-09 2006-05-09
US80641206P 2006-06-30 2006-06-30
US80710406P 2006-07-12 2006-07-12
PCT/US2006/047486 WO2007120241A2 (en) 2006-04-18 2006-12-11 Droplet-based biochemistry

Publications (1)

Publication Number Publication Date
HK1127066A1 true HK1127066A1 (en) 2009-09-18

Family

ID=38609969

Family Applications (2)

Application Number Title Priority Date Filing Date
HK09105694.9A HK1127066A1 (en) 2006-04-18 2009-06-24 Droplet-based biochemistry
HK09106032.8A HK1127091A1 (en) 2006-04-18 2009-07-06 Droplet-based pyrosequencing

Family Applications After (1)

Application Number Title Priority Date Filing Date
HK09106032.8A HK1127091A1 (en) 2006-04-18 2009-07-06 Droplet-based pyrosequencing

Country Status (7)

Country Link
EP (2) EP2016189B1 (xx)
JP (1) JP5054096B2 (xx)
AT (2) ATE542921T1 (xx)
CA (2) CA2680532C (xx)
DE (1) DE602006018794D1 (xx)
HK (2) HK1127066A1 (xx)
WO (2) WO2007120240A2 (xx)

Families Citing this family (218)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
KR101198038B1 (ko) 2005-01-28 2012-11-06 듀크 유니버서티 인쇄 회로 기판 위의 액적 조작을 위한 기구 및 방법
WO2006124458A2 (en) 2005-05-11 2006-11-23 Nanolytics, Inc. Method and device for conducting biochemical or chemical reactions at multiple temperatures
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
DK2001990T3 (en) 2006-03-24 2016-10-03 Handylab Inc Integrated microfluidic sample processing system and method for its use
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
WO2007123908A2 (en) 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Droplet-based multiwell operations
US7727723B2 (en) 2006-04-18 2010-06-01 Advanced Liquid Logic, Inc. Droplet-based pyrosequencing
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
WO2009111769A2 (en) 2008-03-07 2009-09-11 Advanced Liquid Logic, Inc. Reagent and sample preparation and loading on a fluidic device
JP5266208B2 (ja) * 2006-05-09 2013-08-21 アドヴァンスト リキッド ロジック インコーポレイテッド 液滴処置システム
US8296088B2 (en) 2006-06-02 2012-10-23 Luminex Corporation Systems and methods for performing measurements of one or more materials
WO2007143615A2 (en) * 2006-06-02 2007-12-13 Luminex Corporation Systems and methods for performing measurements of one or more analytes comprising using magnetic particles and applying a magnetic field
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
WO2008091848A2 (en) 2007-01-22 2008-07-31 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
WO2008098236A2 (en) * 2007-02-09 2008-08-14 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
US8872527B2 (en) 2007-02-15 2014-10-28 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
WO2008116209A1 (en) 2007-03-22 2008-09-25 Advanced Liquid Logic, Inc. Enzymatic assays for a droplet actuator
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
US8202686B2 (en) 2007-03-22 2012-06-19 Advanced Liquid Logic, Inc. Enzyme assays for a droplet actuator
WO2009002920A1 (en) 2007-06-22 2008-12-31 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
EP2171460B1 (en) 2007-07-13 2017-08-30 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
WO2009029561A2 (en) 2007-08-24 2009-03-05 Advanced Liquid Logic, Inc. Bead manipulations on a droplet actuator
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US8460528B2 (en) 2007-10-17 2013-06-11 Advanced Liquid Logic Inc. Reagent storage and reconstitution for a droplet actuator
WO2009052354A2 (en) 2007-10-17 2009-04-23 Advanced Liquid Logic, Inc. Droplet actuator structures
EP2232535A4 (en) 2007-12-10 2016-04-13 Advanced Liquid Logic Inc DROPLET ACTUATOR CONFIGURATIONS AND METHODS
BRPI0821734A2 (pt) 2007-12-23 2022-10-25 Advanced Liquid Logic Inc Configurações de autuador de gotículas e métodos para conduzir operações de gotícula.
CA2639954C (en) 2008-02-11 2017-08-15 Aaron R. Wheeler Droplet-based cell culture and cell assays using digital microfluidics
CN102027367B (zh) * 2008-02-29 2016-03-23 西北大学 促进生物反应的屏障
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
JP5592355B2 (ja) * 2008-05-13 2014-09-17 アドヴァンスト リキッド ロジック インコーポレイテッド 液滴アクチュエータ装置、システム、および方法
EP2286228B1 (en) 2008-05-16 2019-04-03 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for manipulating beads
JP5504587B2 (ja) * 2008-05-27 2014-05-28 東レ株式会社 分析用チップ
US8364315B2 (en) * 2008-08-13 2013-01-29 Advanced Liquid Logic Inc. Methods, systems, and products for conducting droplet operations
JP2010099061A (ja) * 2008-09-26 2010-05-06 Sekisui Chem Co Ltd 検出用カートリッジ及び被検出物質の検出方法
US8187864B2 (en) 2008-10-01 2012-05-29 The Governing Council Of The University Of Toronto Exchangeable sheets pre-loaded with reagent depots for digital microfluidics
US8053239B2 (en) 2008-10-08 2011-11-08 The Governing Council Of The University Of Toronto Digital microfluidic method for protein extraction by precipitation from heterogeneous mixtures
CA2740113C (en) 2008-10-10 2019-12-24 The Governing Council Of The University Of Toronto Hybrid digital and channel microfluidic devices and methods of use thereof
EP2344887A1 (en) * 2008-10-31 2011-07-20 Koninklijke Philips Electronics N.V. Biosensor with multi-chamber cartridge
US20110311980A1 (en) * 2008-12-15 2011-12-22 Advanced Liquid Logic, Inc. Nucleic Acid Amplification and Sequencing on a Droplet Actuator
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
US9851365B2 (en) 2009-02-26 2017-12-26 The Governing Council Of The University Of Toronto Digital microfluidic liquid-liquid extraction device and method of use thereof
US8202736B2 (en) 2009-02-26 2012-06-19 The Governing Council Of The University Of Toronto Method of hormone extraction using digital microfluidics
WO2011020011A2 (en) * 2009-08-13 2011-02-17 Advanced Liquid Logic, Inc. Droplet actuator and droplet-based techniques
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US8846414B2 (en) 2009-09-29 2014-09-30 Advanced Liquid Logic, Inc. Detection of cardiac markers on a droplet actuator
SE534542C2 (sv) * 2009-09-30 2011-09-27 Calmark Sweden Ab Testsystem för bestämning av hypoxiutlöst cellskada
EP2485844B1 (en) * 2009-10-06 2020-07-22 Koninklijke Philips N.V. Magnetic sample purification
JP5748228B2 (ja) * 2009-10-15 2015-07-15 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 放射化学のためのデジタル微小流体プラットフォーム
WO2011057197A2 (en) 2009-11-06 2011-05-12 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel electrophoresis and molecular analysis
HUE027972T2 (en) * 2010-02-25 2016-11-28 Advanced Liquid Logic Inc A method for generating nucleic acid libraries
US8399198B2 (en) * 2010-03-02 2013-03-19 Bio-Rad Laboratories, Inc. Assays with droplets transformed into capsules
US8734628B2 (en) * 2010-03-10 2014-05-27 Empire Technology Development, Llc Microfluidic channel device with array of drive electrodes
WO2011126892A2 (en) 2010-03-30 2011-10-13 Advanced Liquid Logic, Inc. Droplet operations platform
CN105385756B (zh) 2010-04-05 2020-12-25 普罗格诺西斯生物科学公司 空间编码的生物学测定
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
JP5573335B2 (ja) * 2010-04-28 2014-08-20 株式会社島津製作所 磁性体粒子操作デバイス及び磁性体粒子操作方法
EP2567213B1 (en) 2010-05-05 2018-01-24 The Governing Council of the Universtiy of Toronto Method of processing dried samples using digital microfluidic device
US9011662B2 (en) 2010-06-30 2015-04-21 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
CN105964312A (zh) 2010-07-22 2016-09-28 基因细胞生物系统有限公司 复合液体池
JP5694726B2 (ja) 2010-09-30 2015-04-01 富士フイルム株式会社 検査方法および装置
WO2012040861A1 (en) 2010-10-01 2012-04-05 The Governing Council Of The University Of Toronto Digital microfluidic devices and methods incorporating a solid phase
CA2829185C (en) 2011-03-08 2017-11-14 Japan Science And Technology Agency Bead sealing method, method for detecting target molecule, array, kit, and target molecule detection device
US10018627B2 (en) * 2011-03-08 2018-07-10 Japan Science And Technology Agency Method for sealing substances, method for detecting target molecule, array, kit, and target molecule detection device
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CA3082652A1 (en) 2011-04-15 2012-10-18 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
US8339711B2 (en) * 2011-04-22 2012-12-25 Sharp Kabushiki Kaisha Active matrix device and method of driving the same
CA2833897C (en) 2011-05-09 2020-05-19 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
WO2012154794A2 (en) 2011-05-10 2012-11-15 Advanced Liquid Logic, Inc. Enzyme concentration and assays
EP2729792A4 (en) 2011-07-06 2015-03-18 Advanced Liquid Logic Inc STORING REAGENTS ON A DROPLET HANDLING ACTUATOR
US8901043B2 (en) 2011-07-06 2014-12-02 Advanced Liquid Logic, Inc. Systems for and methods of hybrid pyrosequencing
WO2013009927A2 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based assays
US9446404B2 (en) 2011-07-25 2016-09-20 Advanced Liquid Logic, Inc. Droplet actuator apparatus and system
WO2013066441A2 (en) * 2011-07-29 2013-05-10 The Texas A&M University System Digital microfluidic platform for actuating and heating individual liquid droplets
JP5965908B2 (ja) * 2011-08-22 2016-08-10 パナソニック株式会社 マイクロ流体デバイス
US8691067B2 (en) * 2011-09-16 2014-04-08 International Business Machines Corporation Charged entities as locomotive to control motion of polymers through a nanochannel
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use
WO2013078216A1 (en) 2011-11-21 2013-05-30 Advanced Liquid Logic Inc Glucose-6-phosphate dehydrogenase assays
PL397071A1 (pl) * 2011-11-21 2013-05-27 Scope Fluidics Spólka Z Ograniczona Odpowiedzialnoscia Sposób oznaczania parametrów biochemicznych plynu ustrojowego
WO2013090889A1 (en) * 2011-12-16 2013-06-20 Advanced Liquid Logic Inc Sample preparation on a droplet actuator
JP5807542B2 (ja) 2011-12-22 2015-11-10 株式会社島津製作所 対象成分を操作するためのチップデバイス及びそれを用いた方法
ES2642862T3 (es) * 2012-01-25 2017-11-20 Gencell Biosystems Limited Aislamiento de biomoléculas
US10564147B2 (en) 2012-05-25 2020-02-18 The Regents Of The University Of California Microfluidic systems for particle trapping and separation using cavity acoustic transducers
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
JP6222671B2 (ja) * 2012-06-27 2017-11-01 アドバンスト リキッド ロジック インコーポレイテッドＡｄｖａｎｃｅｄ Ｌｉｑｕｉｄ Ｌｏｇｉｃ， Ｉｎｃ． 泡形成を低減するための技術および液滴アクチュエーターの設計
GB201217772D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Sequencing method
US10000802B2 (en) 2012-10-04 2018-06-19 Base4 Innovation Ltd Sequencing method
US9863913B2 (en) 2012-10-15 2018-01-09 Advanced Liquid Logic, Inc. Digital microfluidics cartridge and system for operating a flow cell
EP2909337B1 (en) 2012-10-17 2019-01-09 Spatial Transcriptomics AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
JP1628116S (xx) * 2012-10-24 2019-04-01
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
CN104812492A (zh) 2012-11-27 2015-07-29 基因细胞生物系统有限公司 处理液体样品
EP2869922B1 (en) * 2013-01-09 2019-11-20 Tecan Trading AG Disposable cartridge for microfluidics systems
US9492824B2 (en) * 2013-01-16 2016-11-15 Sharp Kabushiki Kaisha Efficient dilution method, including washing method for immunoassay
GB201300957D0 (en) * 2013-01-18 2013-03-06 Base4 Innovation Ltd Sequencing method
EP3034171B1 (en) 2013-03-15 2019-04-24 Genmark Diagnostics Inc. Systems, methods and apparatus for manipulating deformable fluid vessels
CN107817232B (zh) * 2013-03-15 2021-08-17 Hycor生物医学有限责任公司 用于进行过敏症和自身免疫性疾病的诊断测定的自动化免疫分析系统
GB201306444D0 (en) * 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
GB201306445D0 (en) * 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
GB201310584D0 (en) * 2013-06-13 2013-07-31 Base4 Innovation Ltd Droplet storage method
CN105339497A (zh) * 2013-06-18 2016-02-17 基因细胞生物系统有限公司 生物分子分离和热加工
DK3013984T3 (da) 2013-06-25 2023-06-06 Prognosys Biosciences Inc Metode til bestemmelse af spatiale mønstre i biologiske targets i en prøve
WO2015023747A1 (en) 2013-08-13 2015-02-19 Advanced Liquid Logic, Inc. Methods of improving accuracy and precision of droplet metering using an on-actuator reservoir as the fluid input
WO2015031849A1 (en) 2013-08-30 2015-03-05 Illumina, Inc. Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
CN107267383A (zh) 2013-12-18 2017-10-20 东洋制罐集团控股株式会社 培养容器的制造方法和固相化装置
ITRM20130700A1 (it) * 2013-12-19 2015-06-20 Stichting Katholieke Univ Metodo per il rilevamento di cellule tumorali circolanti
US10384187B2 (en) 2014-02-10 2019-08-20 Gencell Biosystems Ltd Composite liquid cell (CLC) mediated nucleic acid library preparation device, and methods for using the same
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
CN106459967A (zh) 2014-04-29 2017-02-22 Illumina公司 使用模板转换和标签化的多重单细胞基因表达分析
WO2015188165A1 (en) * 2014-06-06 2015-12-10 The Regents Of The University Of California Self-shielded, benchtop chemistry system
SG11201700133SA (en) 2014-07-08 2017-03-30 Japan Science & Tech Agency Substance sealing method and target molecule detecting method
US10059922B2 (en) 2014-07-31 2018-08-28 Becton, Dickinson And Company Methods and systems for separating components of a biological sample with gravity sedimentation
GB201414177D0 (en) 2014-08-11 2014-09-24 Sphere Fluidics Ltd Droplet Sorting
RU2690346C2 (ru) 2014-10-09 2019-05-31 Иллюмина, Инк. Способ и устройство для разделения несмешиваемых жидкостей с целью эффективного отделения по меньшей мере одной из жидкостей
US11369962B2 (en) 2014-10-24 2022-06-28 National Technology & Engineering Solutions Of Sandia, Llc Method and device for tracking and manipulation of droplets
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
JP6307179B2 (ja) * 2015-02-03 2018-04-04 株式会社日立製作所 単一細胞解析用フローセルデバイス及び単一細胞解析装置
SG11201706504RA (en) 2015-02-10 2017-09-28 Illumina Inc Methods and compositions for analyzing cellular components
EP3822361A1 (en) * 2015-02-20 2021-05-19 Takara Bio USA, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
US10576471B2 (en) 2015-03-20 2020-03-03 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
AU2016243034A1 (en) * 2015-04-03 2017-10-19 Abbott Laboratories Devices and methods for sample analysis
CA2981515A1 (en) * 2015-04-03 2016-10-06 Abbott Laboratories Devices and methods for sample analysis
ES2972835T3 (es) 2015-04-10 2024-06-17 10X Genomics Sweden Ab Análisis multiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
IL293366B2 (en) 2015-04-22 2023-10-01 Berkeley Lights Inc Kits and methods for preparing a microfluidic device for cell culture
KR102175826B1 (ko) 2015-05-11 2020-11-06 일루미나, 인코포레이티드 치료제의 발견 및 분석을 위한 플랫폼
WO2016197106A1 (en) 2015-06-05 2016-12-08 Miroculus Inc. Evaporation management in digital microfluidic devices
WO2016197103A1 (en) 2015-06-05 2016-12-08 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
GB2556713B (en) 2015-07-06 2021-06-23 Illumina Inc Balanced AC modulation for driving droplet operations electrodes
IL295355B2 (en) 2015-08-14 2023-11-01 Illumina Inc System and methods for determining genetic characteristics using magnetically responsive sensors
EP3341494B1 (en) 2015-08-28 2024-08-07 Illumina, Inc. Nucleic acid sequence analysis from single cells
WO2017037078A1 (en) 2015-09-02 2017-03-09 Illumina Cambridge Limited Systems and methods of improving droplet operations in fluidic systems
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
GB2542372A (en) * 2015-09-16 2017-03-22 Sharp Kk Microfluidic device and a method of loading fluid therein
US9862941B2 (en) 2015-10-14 2018-01-09 Pioneer Hi-Bred International, Inc. Single cell microfluidic device
AU2016341948A1 (en) 2015-10-22 2018-05-10 Illumina Cambridge Limited Filler fluid for fluidic devices
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
SG11201804563SA (en) * 2015-12-01 2018-06-28 Illumina Inc Digital microfluidic system for single-cell isolation and characterization of analytes
CN108602066B (zh) 2015-12-01 2021-08-17 亿明达股份有限公司 液体存储输送机构以及方法
CA3016221C (en) 2016-04-07 2021-09-28 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
AU2017271673B2 (en) 2016-05-26 2022-04-14 Berkeley Lights, Inc. Covalently modified surfaces, kits, and methods of preparation and use
JP6829950B2 (ja) * 2016-05-31 2021-02-17 シスメックス株式会社 分析方法、分析装置および分析システム
WO2017210199A1 (en) 2016-05-31 2017-12-07 Oregon State University Fluidic devices for chromatographic separation and methods of making and using the same
GB2551116B (en) 2016-05-31 2019-05-15 Solentim Ltd Dispensing method and apparatus
EP3500660A4 (en) 2016-08-22 2020-03-04 Miroculus Inc. FEEDBACK SYSTEM FOR CONTROLLING PARALLEL DROPLETS IN A DIGITAL MICROFLUIDIC DEVICE
US11198129B2 (en) 2016-10-05 2021-12-14 Abbott Laboratories Devices and methods for sample analysis
CN109863391B (zh) * 2016-10-05 2021-11-05 雅培实验室 用于样品分析的装置和方法
EP3525933B1 (en) * 2016-10-11 2024-07-03 The Regents of the University of California Systems and methods to encapsulate and preserve organic matter for analysis
WO2018126082A1 (en) 2016-12-28 2018-07-05 Miroculis Inc. Digital microfluidic devices and methods
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
WO2018227210A1 (en) 2017-06-09 2018-12-13 The Regents Of The University Of California High-efficiency encapsulation in droplets based on hydrodynamic vortices control
US11517901B2 (en) 2017-06-09 2022-12-06 The Regents Of The University Of California High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting
IL313689A (en) * 2017-06-21 2024-08-01 Lightcast Discovery Ltd Manipulation device for microdroplets
CN110892258A (zh) 2017-07-24 2020-03-17 米罗库鲁斯公司 具有集成的血浆收集设备的数字微流控系统和方法
WO2019046860A1 (en) 2017-09-01 2019-03-07 Miroculus Inc. DIGITAL MICROFLUIDIC DEVICES AND METHODS OF USE
WO2019075409A1 (en) 2017-10-12 2019-04-18 The Regents Of The University Of California ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM)
WO2019079787A1 (en) 2017-10-20 2019-04-25 The Regents Of The University Of California MICROFLUIDIC SYSTEMS AND METHODS FOR CELL TRANSFECTION VIA LIPOFLEX
US11499127B2 (en) 2017-10-20 2022-11-15 The Regents Of The University Of California Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks
CN112041459B (zh) 2018-01-29 2024-09-10 圣祖德儿童研究医院 核酸扩增方法
US11207455B2 (en) * 2018-05-14 2021-12-28 Oregon State University Membrane device for blood separation and methods of making and using the same
CA3096855A1 (en) 2018-05-23 2019-11-28 Miroculus Inc. Control of evaporation in digital microfluidics
CA3108408A1 (en) 2018-08-06 2020-02-13 Nicoya Lifesciences Inc. Plasmon resonance (pr) system, instrument, cartridge, and methods and configurations thereof
JP7571011B2 (ja) * 2018-09-06 2024-10-22 ニコヤ ライフサイエンシーズ インコーポレイテッド プラズモン共鳴（ｐｒ）システムおよび機器、デジタルマイクロ流体（ｄｍｆ）カートリッジ、ならびに検体の分析のために局在表面プラズモン共鳴（ｌｓｐｒ）を使用する方法
JP7022844B2 (ja) * 2018-10-17 2022-02-18 株式会社日立ハイテク 生化学用カートリッジ及び生化学分析装置
CN112585474B (zh) * 2018-10-19 2024-06-21 株式会社日立高新技术 生物化学用盒和生物化学分析装置
EP3693086B1 (en) * 2019-02-08 2020-12-09 Lightcast Discovery Ltd Microdroplet manipulation method
EP3930901A4 (en) * 2019-02-28 2023-01-04 Miroculus Inc. DIGITAL MICROFLUID DEVICES AND METHODS OF USE THEREOF
GB201902810D0 (en) * 2019-03-01 2019-04-17 Vidya Holdings Ltd Disposition of reagents in assay device
WO2020180327A1 (en) * 2019-03-07 2020-09-10 Hewlett-Packard Development Company, L.P. Nucleic acid detection
CA3133124A1 (en) 2019-04-08 2020-10-15 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
US11724260B2 (en) 2019-04-08 2023-08-15 The Regents Of The University Of Michigan Microfluidic sensor
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
KR102308065B1 (ko) * 2019-11-08 2021-09-30 경북대학교 산학협력단 입자검출장치 및 이를 이용한 입자검출방법
WO2021102134A1 (en) 2019-11-20 2021-05-27 E Ink Corporation Spatially variable hydrophobic layers for digital microfluidics
WO2021113065A1 (en) * 2019-12-04 2021-06-10 Becton, Dickinson And Company Magnetic capture bead mediated molecular barcoding of nucleic acid targets in single particles and compositions for use in the same
CN114945426A (zh) 2020-01-17 2022-08-26 核酸有限公司 用于数字微流体的空间可变介电层
US11946901B2 (en) 2020-01-27 2024-04-02 Nuclera Ltd Method for degassing liquid droplets by electrical actuation at higher temperatures
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
JP2023513832A (ja) 2020-02-18 2023-04-03 ヌークレラ ヌクリークス， リミテッド Ｅｗｏｄアレイの高周波ａｃ駆動のためのアダプティブゲート駆動
JP2023514278A (ja) 2020-02-19 2023-04-05 ヌークレラ ヌクリークス， リミテッド ＥＷｏＤアレイの高周波数ＡＣ駆動のためのラッチ付きトランジスタ駆動
WO2021222061A1 (en) 2020-04-27 2021-11-04 Nuclera Nucleics Ltd. Segmented top plate for variable driving and short protection for digital microfluidics
US20230176047A1 (en) * 2020-04-29 2023-06-08 Abbott Laboratories Systems and methods for sample analysis
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2022038304A1 (en) * 2020-08-19 2022-02-24 Aalto University Foundation Sr Method of characterizing the properties of a surface
WO2022054700A1 (ja) * 2020-09-10 2022-03-17 ニプロ株式会社 血液検査装置及び血液検査方法
TWI781660B (zh) * 2020-09-30 2022-10-21 富佳生技股份有限公司 核酸檢測盒及核酸檢測設備
JP2022058244A (ja) * 2020-09-30 2022-04-11 富佳生技股▲ふん▼有限公司 核酸検出箱及び核酸検出デバイス
TW202228845A (zh) 2020-10-08 2022-08-01 英商核酸有限公司 微流體系統中試劑特異驅動ewod（介電質上電潤濕）陣列的方法
CA3198842A1 (en) 2020-10-21 2022-04-28 Illumina, Inc. Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
WO2022096882A1 (en) 2020-11-04 2022-05-12 Nuclera Nucleics Ltd Dielectric layers for digital microfluidic devices
GB2602105A (en) * 2020-12-18 2022-06-22 Solentim Ltd Cell dispenser
US20220236249A1 (en) * 2021-01-25 2022-07-28 Koninklijke Philips N.V. Apparatus and method for sputum conditioning and analysis
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
EP4387768A2 (en) * 2021-08-18 2024-06-26 Nuclera Ltd Methods and compositions for improved biomolecule assays on digital microfluidic devices
EP4426489A2 (en) * 2021-11-03 2024-09-11 Abbott Laboratories Systems and methods for sample analysis
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6319668B1 (en) * 1995-04-25 2001-11-20 Discovery Partners International Method for tagging and screening molecules
US5851769A (en) * 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
US6379929B1 (en) * 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
FI980874A (fi) * 1998-04-20 1999-10-21 Wallac Oy Menetelmä ja laite pienten nestemäärien kemiallisen analyysin suorittamiseksi
US6226082B1 (en) * 1998-06-25 2001-05-01 Amira Medical Method and apparatus for the quantitative analysis of a liquid sample with surface enhanced spectroscopy
US20030012699A1 (en) * 1998-11-18 2003-01-16 Thomas Moore Simultaneous handling of magnetic beads in a two-dimensional arrangement
US6225061B1 (en) * 1999-03-10 2001-05-01 Sequenom, Inc. Systems and methods for performing reactions in an unsealed environment
US6432290B1 (en) * 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US6442413B1 (en) * 2000-05-15 2002-08-27 James H. Silver Implantable sensor
AU2001290879A1 (en) * 2000-09-15 2002-03-26 California Institute Of Technology Microfabricated crossflow devices and methods
US6844028B2 (en) * 2001-06-26 2005-01-18 Accelr8 Technology Corporation Functional surface coating
US20030119057A1 (en) * 2001-12-20 2003-06-26 Board Of Regents Forming and modifying dielectrically-engineered microparticles
US6911132B2 (en) * 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7217542B2 (en) * 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
FR2846957B1 (fr) * 2002-11-13 2005-09-30 Commissariat Energie Atomique Micro-systeme a remplissage de micro-billes et procede d'obtention
US20040239080A1 (en) * 2003-05-30 2004-12-02 Berrahou Philip F. Housing retention mechanism
TWI230760B (en) * 2003-07-29 2005-04-11 Univ Tsinghua Electrowetting electrode design with electromagnetic field for actuation of the magnetic-beads biochemical detection system
US7767435B2 (en) * 2003-08-25 2010-08-03 University Of Washington Method and device for biochemical detection and analysis of subcellular compartments from a single cell
CA2479452C (en) * 2003-08-30 2008-11-04 F.Hoffmann-La Roche Ag Method and device for determining analytes in a liquid
KR100647282B1 (ko) * 2003-12-24 2006-11-17 삼성전자주식회사 ＤＮＡ 마이크로어레이 스폿팅(Ｓｐｏｔｉｎｇ) 장치 및이를 이용한 스폿팅 방법
KR20070037432A (ko) * 2004-01-15 2007-04-04 도꾸리쯔교세이호징 가가꾸 기쥬쯔 신꼬 기꼬 화학 분석장치 및 화학 분석방법
CA2553833C (en) * 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
KR100583231B1 (ko) * 2004-04-13 2006-05-26 한국과학기술연구원 물방울형 세포 부유액을 이용한 세포 분리 장치
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7300993B2 (en) * 2004-06-03 2007-11-27 Shell Oil Company Process for the preparation of polyether polyols
FR2871076A1 (fr) * 2004-06-04 2005-12-09 Univ Lille Sciences Tech Dispositif pour desorption par rayonnement laser incorporant une manipulation de l'echantillon liquide sous forme de gouttes individuelles permettant leur traitement chimique et biochimique
US7693666B2 (en) * 2004-07-07 2010-04-06 Rensselaer Polytechnic Institute Method, system, and program product for controlling chemical reactions in a digital microfluidic system
JP4047314B2 (ja) * 2004-09-07 2008-02-13 株式会社東芝 微細流路構造体

Also Published As

Publication number Publication date
EP2016091B1 (en) 2010-12-08
JP5054096B2 (ja) 2012-10-24
CA2680532A1 (en) 2007-10-25
EP2016189B1 (en) 2012-01-25
WO2007120240A3 (en) 2008-08-28
WO2007120241A2 (en) 2007-10-25
WO2007120240A2 (en) 2007-10-25
EP2016091A2 (en) 2009-01-21
EP2016189A2 (en) 2009-01-21
EP2016091A4 (en) 2009-09-09
WO2007120241A3 (en) 2008-08-28
CA2680061A1 (en) 2007-10-25
ATE542921T1 (de) 2012-02-15
CA2680532C (en) 2017-03-21
EP2016189A4 (en) 2010-12-08
HK1127091A1 (en) 2009-09-18
JP2009534653A (ja) 2009-09-24
ATE490971T1 (de) 2010-12-15
CA2680061C (en) 2015-10-13
DE602006018794D1 (de) 2011-01-20

Similar Documents

Publication Publication Date Title
HK1127066A1 (en) Droplet-based biochemistry
WO2008109176A8 (en) Assays and other reactions involving droplets
EP2044191A4 (en) SYSTEMS AND METHOD FOR MONITORING THE AMPLIFICATION AND DISSOCIATION BEHAVIOR OF DNA MOLECULES
WO2008024319A3 (en) Microfluidic devices for nucleic acid assembly
CA2555060C (en) Method and device for manipulating liquids in microfluidic systems
Kodzius et al. Inhibitory effect of common microfluidic materials on PCR outcome
WO2010117420A3 (en) Fret-labeled compounds and uses therefor
WO2010026488A3 (en) Methods and kits for nucleic acid sequencing
WO2007041342A3 (en) Labeled nucleotide analogs and uses therefor
WO2008005248A3 (en) Real-time pcr in micro-channels
WO2001034302A3 (en) Biochannel assay for hybridization with biomaterial
WO2004103565A3 (de) Vorrichtung und verfahren zur strukturierung von flüssigkeiten und zum zudosieren von reaktionsflüssigkeiten zu in separationsmedium eingebetteten flüssigkeitskompartimenten
WO2008149346A3 (en) Enhanced sensitivity polymerase chain reactions
WO2006127191A3 (en) Analysis engine and database for manipulating parameters for fluidic systems on a chip
WO2003056030A3 (en) Methods and systems of nucleic acid sequencing
WO2010091246A3 (en) Burstable liquid packaging and uses thereof
WO2001041931A3 (en) Multilayered microfluidic devices for analyte reactions
WO2008063227A3 (en) Microfluidic devices
BRPI0913107A2 (pt) Reagente de lise, ligação e/ou lavagem, uso do mesmo, método e kit para isolamento e/ou purificação de ácidos nucleicos de uma amostra biológica contendo ácidos nucleicos, e, uso de tensoativos não-iônicos com base em polixietileno.
WO2007024798A3 (en) Apparatus, system, and method using immiscible-fluid-discrete-volumes
WO2009092564A3 (en) Integrated instrument performing synthesis and amplification
WO2007123744A3 (en) Systems and devices for sequence by synthesis analysis
EA201290612A1 (ru) Способ выделения нуклеиновой кислоты и набор для этого
WO2008133640A3 (en) Disposable micropurification cards, methods, and systems thereof
WO2007057167A3 (en) A method for enhancing enzymatic dna polymerase reactions