ATE490971T1 - Biochemie auf tröpfchenbasis - Google Patents

Biochemie auf tröpfchenbasis

Info

Publication number
ATE490971T1
ATE490971T1 AT06849949T AT06849949T ATE490971T1 AT E490971 T1 ATE490971 T1 AT E490971T1 AT 06849949 T AT06849949 T AT 06849949T AT 06849949 T AT06849949 T AT 06849949T AT E490971 T1 ATE490971 T1 AT E490971T1
Authority
AT
Austria
Prior art keywords
protocols
droplet microactuator
reagents
droplets
biochemistry
Prior art date
Application number
AT06849949T
Other languages
English (en)
Inventor
Michael Pollack
Vamsee Pamula
Vijay Srinivasan
Philip Paik
Allen Eckhardt
Richard Fair
Original Assignee
Advanced Liquid Logic Inc
Univ Duke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Liquid Logic Inc, Univ Duke filed Critical Advanced Liquid Logic Inc
Application granted granted Critical
Publication of ATE490971T1 publication Critical patent/ATE490971T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3031—Micromixers using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0436—Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet
    • B01L2400/0448—Marangoni flow; Thermocapillary effect
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Saccharide Compounds (AREA)
  • Colloid Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT06849949T 2006-04-18 2006-12-11 Biochemie auf tröpfchenbasis ATE490971T1 (de)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US74505806P 2006-04-18 2006-04-18
US74507306P 2006-04-18 2006-04-18
US74503906P 2006-04-18 2006-04-18
US74505906P 2006-04-18 2006-04-18
US74591406P 2006-04-28 2006-04-28
US74595006P 2006-04-28 2006-04-28
US74679706P 2006-05-09 2006-05-09
US74680106P 2006-05-09 2006-05-09
US80641206P 2006-06-30 2006-06-30
US80710406P 2006-07-12 2006-07-12
PCT/US2006/047486 WO2007120241A2 (en) 2006-04-18 2006-12-11 Droplet-based biochemistry

Publications (1)

Publication Number Publication Date
ATE490971T1 true ATE490971T1 (de) 2010-12-15

Family

ID=38609969

Family Applications (2)

Application Number Title Priority Date Filing Date
AT06849949T ATE490971T1 (de) 2006-04-18 2006-12-11 Biochemie auf tröpfchenbasis
AT06847588T ATE542921T1 (de) 2006-04-18 2006-12-11 Pyrosequenzierung auf tröpfchenbasis

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT06847588T ATE542921T1 (de) 2006-04-18 2006-12-11 Pyrosequenzierung auf tröpfchenbasis

Country Status (6)

Country Link
EP (2) EP2016189B1 (de)
JP (1) JP5054096B2 (de)
AT (2) ATE490971T1 (de)
CA (2) CA2680532C (de)
DE (1) DE602006018794D1 (de)
WO (2) WO2007120240A2 (de)

Families Citing this family (233)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005011867A2 (en) 2003-07-31 2005-02-10 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
EP1859330B1 (de) 2005-01-28 2012-07-04 Duke University Vorrichtungen und verfahren zur manipulation von tropfen auf einer leiterplatte
EP1885885A4 (de) 2005-05-11 2008-08-27 Nanolytics Inc Verfahren und vorrichtung zur ausführung biochemischer oder chemischer reaktionen bei mehreren temperaturen
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
ES2587007T3 (es) 2006-03-24 2016-10-20 Handylab, Inc. Sistema integrado para procesar muestras microfluídicas, y métodos de uso del mismo
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7727723B2 (en) 2006-04-18 2010-06-01 Advanced Liquid Logic, Inc. Droplet-based pyrosequencing
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
WO2007123908A2 (en) 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Droplet-based multiwell operations
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
US8927296B2 (en) 2006-04-18 2015-01-06 Advanced Liquid Logic, Inc. Method of reducing liquid volume surrounding beads
WO2009111769A2 (en) 2008-03-07 2009-09-11 Advanced Liquid Logic, Inc. Reagent and sample preparation and loading on a fluidic device
EP2021103B1 (de) * 2006-05-09 2017-07-12 Advanced Liquid Logic, Inc. Elektrobenetzungströpfchenmikroaktuator gesteuert mit grafischer Benutzeroberfläche
US8889347B2 (en) * 2006-06-02 2014-11-18 Luminex Corporation Systems and methods for performing measurements of one or more materials
US8296088B2 (en) 2006-06-02 2012-10-23 Luminex Corporation Systems and methods for performing measurements of one or more materials
EP2091647A2 (de) 2006-11-14 2009-08-26 Handylab, Inc. Mikrofluidisches system für parallele amplifikation und erkennung von polynukleotiden
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
ES2423930T3 (es) * 2007-02-09 2013-09-25 Advanced Liquid Logic, Inc. Dispositivos actuadores de gotitas y métodos que emplean perlas magnéticas
US8872527B2 (en) 2007-02-15 2014-10-28 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
US8440392B2 (en) 2007-03-22 2013-05-14 Advanced Liquid Logic Inc. Method of conducting a droplet based enzymatic assay
US8202686B2 (en) 2007-03-22 2012-06-19 Advanced Liquid Logic, Inc. Enzyme assays for a droplet actuator
US8951732B2 (en) 2007-06-22 2015-02-10 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
WO2009012185A1 (en) 2007-07-13 2009-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
WO2009029561A2 (en) 2007-08-24 2009-03-05 Advanced Liquid Logic, Inc. Bead manipulations on a droplet actuator
WO2009032863A2 (en) 2007-09-04 2009-03-12 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US8460528B2 (en) 2007-10-17 2013-06-11 Advanced Liquid Logic Inc. Reagent storage and reconstitution for a droplet actuator
US8454905B2 (en) 2007-10-17 2013-06-04 Advanced Liquid Logic Inc. Droplet actuator structures
WO2009076414A2 (en) 2007-12-10 2009-06-18 Advanced Liquid Logic, Inc. Droplet actuator configurations and methods
EP2237955A4 (de) 2007-12-23 2016-04-20 Advanced Liquid Logic Inc Tropfenaktuatorkonfigurationen und verfahren zum durchführen von tropfenbetrieb
CA2639954C (en) 2008-02-11 2017-08-15 Aaron R. Wheeler Droplet-based cell culture and cell assays using digital microfluidics
JP5390540B2 (ja) * 2008-02-29 2014-01-15 ノースウエスタン ユニバーシティ 生物反応促進用バリア
WO2009137415A2 (en) 2008-05-03 2009-11-12 Advanced Liquid Logic, Inc. Reagent and sample preparation, loading, and storage
JP5592355B2 (ja) * 2008-05-13 2014-09-17 アドヴァンスト リキッド ロジック インコーポレイテッド 液滴アクチュエータ装置、システム、および方法
JP5504587B2 (ja) * 2008-05-27 2014-05-28 東レ株式会社 分析用チップ
WO2010019782A2 (en) * 2008-08-13 2010-02-18 Advanced Liquid Logic, Inc. Methods, systems, and products for conducting droplet operations
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
JP2010099061A (ja) * 2008-09-26 2010-05-06 Sekisui Chem Co Ltd 検出用カートリッジ及び被検出物質の検出方法
US8187864B2 (en) 2008-10-01 2012-05-29 The Governing Council Of The University Of Toronto Exchangeable sheets pre-loaded with reagent depots for digital microfluidics
US8053239B2 (en) 2008-10-08 2011-11-08 The Governing Council Of The University Of Toronto Digital microfluidic method for protein extraction by precipitation from heterogeneous mixtures
US9039973B2 (en) 2008-10-10 2015-05-26 The Governing Council Of The University Of Toronto Hybrid digital and channel microfluidic devices and methods of use thereof
JP2012507703A (ja) * 2008-10-31 2012-03-29 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ マルチチャンバー・カートリッジを有するバイオセンサー
WO2010077859A2 (en) * 2008-12-15 2010-07-08 Advanced Liquid Logic, Inc. Nucleic acid amplification and sequencing on a droplet actuator
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
US8202736B2 (en) 2009-02-26 2012-06-19 The Governing Council Of The University Of Toronto Method of hormone extraction using digital microfluidics
US9851365B2 (en) 2009-02-26 2017-12-26 The Governing Council Of The University Of Toronto Digital microfluidic liquid-liquid extraction device and method of use thereof
US20130288254A1 (en) * 2009-08-13 2013-10-31 Advanced Liquid Logic, Inc. Droplet Actuator and Droplet-Based Techniques
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US8846414B2 (en) 2009-09-29 2014-09-30 Advanced Liquid Logic, Inc. Detection of cardiac markers on a droplet actuator
SE534542C2 (sv) * 2009-09-30 2011-09-27 Calmark Sweden Ab Testsystem för bestämning av hypoxiutlöst cellskada
KR20120093258A (ko) * 2009-10-06 2012-08-22 코닌클리케 필립스 일렉트로닉스 엔.브이. 샘플 정화
EP2488293A4 (de) 2009-10-15 2018-05-23 The Regents of The University of California Digitale mikrofluidische plattform für die radiochemie
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
EP2516669B1 (de) 2009-12-21 2016-10-12 Advanced Liquid Logic, Inc. Enzymassays auf einem tropfenaktuator
PL2539450T3 (pl) * 2010-02-25 2016-08-31 Advanced Liquid Logic Inc Sposób wytwarzania bibliotek kwasu nukleinowego
US8734628B2 (en) 2010-03-10 2014-05-27 Empire Technology Development, Llc Microfluidic channel device with array of drive electrodes
WO2011126892A2 (en) 2010-03-30 2011-10-13 Advanced Liquid Logic, Inc. Droplet operations platform
US9371598B2 (en) 2010-04-05 2016-06-21 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
JP5573335B2 (ja) * 2010-04-28 2014-08-20 株式会社島津製作所 磁性体粒子操作デバイス及び磁性体粒子操作方法
WO2011137533A1 (en) 2010-05-05 2011-11-10 The Governing Council Of The University Of Toronto Method of processing dried samples using digital microfluidic device
US9011662B2 (en) 2010-06-30 2015-04-21 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
ES2669870T3 (es) 2010-07-22 2018-05-29 Gencell Biosystems Limited Células de líquido compuesto
JP5694726B2 (ja) 2010-09-30 2015-04-01 富士フイルム株式会社 検査方法および装置
CA2813090C (en) 2010-10-01 2019-11-12 The Governing Council Of The University Of Toronto Digital microfluidic devices and methods incorporating a solid phase
US10018627B2 (en) * 2011-03-08 2018-07-10 Japan Science And Technology Agency Method for sealing substances, method for detecting target molecule, array, kit, and target molecule detection device
WO2012121310A1 (ja) 2011-03-08 2012-09-13 独立行政法人科学技術振興機構 ビーズ封入方法、ターゲット分子を検出する方法、アレイ、キット及びターゲット分子検出装置
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
ES2617599T3 (es) 2011-04-15 2017-06-19 Becton, Dickinson And Company Termociclador microfluídico de exploración en tiempo real y métodos para termociclado sincronizado y detección óptica de exploración
US8339711B2 (en) * 2011-04-22 2012-12-25 Sharp Kabushiki Kaisha Active matrix device and method of driving the same
US9188615B2 (en) 2011-05-09 2015-11-17 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
CN103597356A (zh) 2011-05-10 2014-02-19 先进流体逻辑公司 酶浓缩与分析
AU2012279420A1 (en) 2011-07-06 2014-01-30 Advanced Liquid Logic Inc Reagent storage on a droplet actuator
US8901043B2 (en) 2011-07-06 2014-12-02 Advanced Liquid Logic, Inc. Systems for and methods of hybrid pyrosequencing
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
WO2013016413A2 (en) 2011-07-25 2013-01-31 Advanced Liquid Logic Inc Droplet actuator apparatus and system
US8980075B2 (en) 2011-07-29 2015-03-17 The Texas A & M University System Digital microfluidic platform for actuating and heating individual liquid droplets
JP5965908B2 (ja) * 2011-08-22 2016-08-10 パナソニック株式会社 マイクロ流体デバイス
US8691067B2 (en) * 2011-09-16 2014-04-08 International Business Machines Corporation Charged entities as locomotive to control motion of polymers through a nanochannel
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
EP2776165A2 (de) 2011-11-07 2014-09-17 Illumina, Inc. Integrierte sequenzierungsvorrichtungen und verwendungsverfahren
WO2013078216A1 (en) 2011-11-21 2013-05-30 Advanced Liquid Logic Inc Glucose-6-phosphate dehydrogenase assays
PL397071A1 (pl) * 2011-11-21 2013-05-27 Scope Fluidics Spólka Z Ograniczona Odpowiedzialnoscia Sposób oznaczania parametrów biochemicznych plynu ustrojowego
WO2013090889A1 (en) * 2011-12-16 2013-06-20 Advanced Liquid Logic Inc Sample preparation on a droplet actuator
JP5807542B2 (ja) 2011-12-22 2015-11-10 株式会社島津製作所 対象成分を操作するためのチップデバイス及びそれを用いた方法
ES2642862T3 (es) * 2012-01-25 2017-11-20 Gencell Biosystems Limited Aislamiento de biomoléculas
WO2013177560A1 (en) 2012-05-25 2013-11-28 The Regents Of The University Of California Microfluidic systems for particle trapping and separation
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
CA2877950C (en) 2012-06-27 2021-06-22 Advanced Liquid Logic Inc. Techniques and droplet actuator designs for reducing bubble formation
GB201217772D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Sequencing method
US10000802B2 (en) 2012-10-04 2018-06-19 Base4 Innovation Ltd Sequencing method
US9863913B2 (en) 2012-10-15 2018-01-09 Advanced Liquid Logic, Inc. Digital microfluidics cartridge and system for operating a flow cell
EP3511423B2 (de) 2012-10-17 2024-05-29 Spatial Transcriptomics AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
EP3919174A3 (de) * 2012-10-24 2022-03-09 Genmark Diagnostics Inc. Integrierte multiplex-zielanalyse
US9776182B2 (en) 2012-11-27 2017-10-03 Gencell Biosystems Ltd. Handling liquid samples
JP6033959B2 (ja) * 2013-01-09 2016-11-30 テカン・トレーディング・アクチェンゲゼルシャフトＴＥＣＡＮ Ｔｒａｄｉｎｇ ＡＧ マイクロ流体システム用使い捨てカートリッジ
US9492824B2 (en) * 2013-01-16 2016-11-15 Sharp Kabushiki Kaisha Efficient dilution method, including washing method for immunoassay
GB201300957D0 (en) * 2013-01-18 2013-03-06 Base4 Innovation Ltd Sequencing method
EP2972230B1 (de) 2013-03-15 2021-07-21 Hycor Biomedical LLC Vorrichtung und zugehöriges verfahren zur durchführung von lumineszenz- und fluoreszenzmessungen in einer probe
JP6351702B2 (ja) 2013-03-15 2018-07-04 ジェンマーク ダイアグノスティクス， インコーポレイテッド 変形可能流体容器を操作するためのシステム、方法、および装置
GB201306444D0 (en) * 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
GB201306445D0 (en) * 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
GB201310584D0 (en) * 2013-06-13 2013-07-31 Base4 Innovation Ltd Droplet storage method
KR20160020559A (ko) * 2013-06-18 2016-02-23 젠셀 바이오시스템즈 리미티드 생체분자 단리 및 열가공
LT3013983T (lt) 2013-06-25 2023-05-10 Prognosys Biosciences, Inc. Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį
EP3033599A4 (de) 2013-08-13 2017-03-22 Advanced Liquid Logic, Inc. Verfahren zur verbesserung der genauigkeit und präzision der tröpfchendosierung unter verwendung eines behälters auf einem aktuator als flüssigkeitseingabe
KR20160081896A (ko) 2013-08-30 2016-07-08 일루미나, 인코포레이티드 친수성 또는 다양한-친수성 표면 상에서의 액적의 조작
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
EP3085767B1 (de) 2013-12-18 2018-10-31 Toyo Seikan Group Holdings, Ltd. Kulturgefäss und verfahren zur kultivierung von lymphozyten
ITRM20130700A1 (it) * 2013-12-19 2015-06-20 Stichting Katholieke Univ Metodo per il rilevamento di cellule tumorali circolanti
WO2015120398A1 (en) 2014-02-10 2015-08-13 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
WO2015168161A2 (en) 2014-04-29 2015-11-05 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
WO2015188165A1 (en) * 2014-06-06 2015-12-10 The Regents Of The University Of California Self-shielded, benchtop chemistry system
WO2016006208A1 (ja) 2014-07-08 2016-01-14 国立研究開発法人科学技術振興機構 物質封入方法及びターゲット分子を検出する方法
WO2016018726A1 (en) 2014-07-31 2016-02-04 Becton, Dickinson And Company Methods and systems for separating components of a biological sample with gravity sedimentation
GB201414177D0 (en) * 2014-08-11 2014-09-24 Sphere Fluidics Ltd Droplet Sorting
BR112017005038B1 (pt) 2014-10-09 2022-01-25 Illumina, Inc Método e dispositivo para separar líquidos imiscíveis para isolar efetivamente pelo menos um dos líquidos
US11369962B2 (en) 2014-10-24 2022-06-28 National Technology & Engineering Solutions Of Sandia, Llc Method and device for tracking and manipulation of droplets
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
WO2016125251A1 (ja) * 2015-02-03 2016-08-11 株式会社日立製作所 単一細胞解析用フローセルデバイス及び単一細胞解析装置
EP3725893A1 (de) 2015-02-10 2020-10-21 Illumina, Inc. Zusammensetzungen zur analyse von zellkomponenten
EP3822361A1 (de) * 2015-02-20 2021-05-19 Takara Bio USA, Inc. Verfahren zur schnellen präzisen abgabe, visualisierung und analyse von einzelzellen
EP3271073B1 (de) 2015-03-20 2019-06-12 Illumina, Inc. Fluidikkassette zur verwendung in vertikaler oder weitgehend vertikaler position
AU2016243036B2 (en) 2015-04-03 2022-02-17 Abbott Laboratories Devices and methods for sample analysis
BR112017021256A2 (pt) * 2015-04-03 2018-06-26 Abbott Laboratories dispositivos e métodos para a análise de amostras
SG11201707515SA (en) 2015-04-10 2017-10-30 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10723988B2 (en) 2015-04-22 2020-07-28 Berkeley Lights, Inc. Microfluidic cell culture
ES2826880T3 (es) 2015-05-11 2021-05-19 Illumina Inc Plataforma para el descubrimiento y análisis de agentes terapéuticos
WO2016197106A1 (en) 2015-06-05 2016-12-08 Miroculus Inc. Evaporation management in digital microfluidic devices
EP3303547A4 (de) 2015-06-05 2018-12-19 Miroculus Inc. Vorrichtung und verfahren für digitale luftmatrix-mikrofluidik zur begrenzung der verdampfung und oberflächenverunreinigung
US10857537B2 (en) 2015-07-06 2020-12-08 Illumina, Inc. Balanced AC modulation for driving droplet operations electrodes
IL295355B2 (en) 2015-08-14 2023-11-01 Illumina Inc Systems And Methods Using Magnetically-Responsive Sensors For Determining A Genetic Characteristic
ES2988429T3 (es) 2015-08-28 2024-11-20 Illumina Inc Análisis de secuencia de ácidos nucleicos de células individuales
CN107921432A (zh) 2015-09-02 2018-04-17 伊卢米纳剑桥有限公司 改善流控系统中的液滴操作的系统和方法
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
GB2542372A (en) * 2015-09-16 2017-03-22 Sharp Kk Microfluidic device and a method of loading fluid therein
US9862941B2 (en) 2015-10-14 2018-01-09 Pioneer Hi-Bred International, Inc. Single cell microfluidic device
US20190217300A1 (en) 2015-10-22 2019-07-18 Illumina, Inc. Filler fluid for fluidic devices
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
CA3006985C (en) * 2015-12-01 2021-11-09 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes
WO2017095845A1 (en) 2015-12-01 2017-06-08 Illumina, Inc. Liquid storage and delivery mechanisms and methods
CN109312396A (zh) 2016-04-07 2019-02-05 伊鲁米那股份有限公司 用于构建标准化核酸文库的方法和系统
SG11201809539RA (en) 2016-05-26 2018-12-28 Berkeley Lights Inc Covalently modified surfaces, kits, and methods of preparation and use
JP6829950B2 (ja) * 2016-05-31 2021-02-17 シスメックス株式会社 分析方法、分析装置および分析システム
WO2017210199A1 (en) 2016-05-31 2017-12-07 Oregon State University Fluidic devices for chromatographic separation and methods of making and using the same
GB2551116B (en) 2016-05-31 2019-05-15 Solentim Ltd Dispensing method and apparatus
EP3500660A4 (de) 2016-08-22 2020-03-04 Miroculus Inc. Rückkopplungssystem für parallele tröpfchensteuerung in einer digitalen mikrofluidischen vorrichtung
CA3029276C (en) 2016-10-05 2023-03-07 Abbott Laboratories Devices and methods for sample analysis
EP3523640B1 (de) * 2016-10-05 2023-01-18 Abbott Laboratories Vorrichtungen zur probenanalyse
US11090653B2 (en) 2016-10-11 2021-08-17 The Regents Of The University Of California Systems and methods to encapsulate and preserve organic matter for analysis
JP2020515815A (ja) 2016-12-28 2020-05-28 ミロキュラス インコーポレイテッド デジタルマイクロ流体デバイスおよび方法
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
US11517901B2 (en) 2017-06-09 2022-12-06 The Regents Of The University Of California High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting
US10780438B2 (en) 2017-06-09 2020-09-22 The Regents Of The University Of California High-efficiency encapsulation in droplets based on hydrodynamic vortices control
IL271537B2 (en) * 2017-06-21 2024-11-01 Base4 Innovation Ltd Microdroplet manipulation device
WO2019023133A1 (en) 2017-07-24 2019-01-31 Miroculus Inc. DIGITAL MICROFLUIDIC SYSTEMS AND METHODS WITH INTEGRATED PLASMA COLLECTION DEVICE
CN115582155B (zh) 2017-09-01 2025-08-26 因特格拉生物科学股份公司 数字微流控设备及其使用方法
WO2019075409A1 (en) 2017-10-12 2019-04-18 The Regents Of The University Of California ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM)
US11745179B2 (en) 2017-10-20 2023-09-05 The Regents Of The University Of California Microfluidic systems and methods for lipoplex-mediated cell transfection
US11499127B2 (en) 2017-10-20 2022-11-15 The Regents Of The University Of California Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks
US11905553B2 (en) 2018-01-29 2024-02-20 St. Jude Children's Research Hospital, Inc. Method for nucleic acid amplification
US11207455B2 (en) * 2018-05-14 2021-12-28 Oregon State University Membrane device for blood separation and methods of making and using the same
EP3796999A4 (de) 2018-05-23 2022-03-09 Miroculus Inc. Steuerung der verdampfung in digitaler mikrofluidik
EP3833955A4 (de) 2018-08-06 2022-04-27 Nicoya Lifesciences, Inc. Plasmonresonanz(pr)-system, instrument, kassette und verfahren und konfigurationen dafür
WO2020049524A1 (en) * 2018-09-06 2020-03-12 Nicoya Lifesciences, Inc. Plasmon resonance (pr) system and instrument, digital microfluidic (dmf) cartridge, and methods of using localized surface plasmon resonance (lspr) for analysis of analytes
US12257576B2 (en) 2018-10-17 2025-03-25 Hitachi High-Tech Corporation Biochemical cartridge and biochemical analysis device
DE112018007860T5 (de) * 2018-10-19 2021-04-29 Hitachi High-Tech Corporation Biochemische Kassette und biochemische Analysevorrichtung
EP3917670A4 (de) 2019-01-31 2022-11-02 Miroculus Inc. Nichtverschmutzende zusammensetzungen und verfahren zum manipulieren und verarbeiten von eingekapselten tröpfchen
ES2841907T3 (es) * 2019-02-08 2021-07-12 Lightcast Discovery Ltd Método de manipulación de microgotitas
WO2020176816A1 (en) * 2019-02-28 2020-09-03 Miroculus Inc. Digital microfluidics devices and methods of using them
GB201902810D0 (en) * 2019-03-01 2019-04-17 Vidya Holdings Ltd Disposition of reagents in assay device
US20220073964A1 (en) * 2019-03-07 2022-03-10 Hewlett-Packard Development Company, L.P. Nucleic acid detection
US11724260B2 (en) 2019-04-08 2023-08-15 The Regents Of The University Of Michigan Microfluidic sensor
CA3133124A1 (en) 2019-04-08 2020-10-15 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
JP7581258B2 (ja) 2019-07-10 2024-11-12 ナショナル リサーチ カウンシル オブ カナダ オイル封入デジタルマイクロ流体におけるオイル残渣保護
WO2021016614A1 (en) 2019-07-25 2021-01-28 Miroculus Inc. Digital microfluidics devices and methods of use thereof
US12179199B2 (en) 2019-08-09 2024-12-31 The Regents Of The University Of California Microfluidic single-cell pairing array for studying cell-cell interactions in isolated compartments
US20210069701A1 (en) * 2019-09-10 2021-03-11 Bgi Shenzhen Co., Ltd. Operation of magnetic beads on microfluidics substrates
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
KR102308065B1 (ko) * 2019-11-08 2021-09-30 경북대학교 산학협력단 입자검출장치 및 이를 이용한 입자검출방법
CN113115586B (zh) * 2019-11-13 2022-12-02 京东方科技集团股份有限公司 检测芯片及其制备方法和使用方法、反应系统
WO2021102134A1 (en) 2019-11-20 2021-05-27 E Ink Corporation Spatially variable hydrophobic layers for digital microfluidics
EP4069867B1 (de) * 2019-12-04 2025-08-27 Becton, Dickinson and Company Durch magnetische einfangkügelchen vermittelte molekulare barcodierung von nukleinsäurezielen in einzelnen partikeln und zusammensetzungen zur verwendung in derselben
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
CN114945426A (zh) 2020-01-17 2022-08-26 核酸有限公司 用于数字微流体的空间可变介电层
US11946901B2 (en) 2020-01-27 2024-04-02 Nuclera Ltd Method for degassing liquid droplets by electrical actuation at higher temperatures
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11410620B2 (en) 2020-02-18 2022-08-09 Nuclera Nucleics Ltd. Adaptive gate driving for high frequency AC driving of EWoD arrays
JP2023514278A (ja) 2020-02-19 2023-04-05 ヌークレラ ヌクリークス， リミテッド ＥＷｏＤアレイの高周波数ＡＣ駆動のためのラッチ付きトランジスタ駆動
EP4142942A4 (de) 2020-04-27 2024-05-22 Nuclera Ltd Segmentierte deckplatte für variablen antrieb und kurzschlussschutz für digitale mikrofluidik
WO2021222667A1 (en) * 2020-04-29 2021-11-04 Abbott Laboratories Systems and methods for sample analysis
EP4153984A1 (de) 2020-05-19 2023-03-29 10X Genomics, Inc. Elektrophoresekassetten und -instrumentierung
ES2989052T3 (es) 2020-05-22 2024-11-25 10X Genomics Inc Medición espacio-temporal simultánea de la expresión génica y la actividad celular
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
US20230304909A1 (en) * 2020-08-19 2023-09-28 Aalto University Foundation Sr Method of characterizing the properties of a surface
CA3190440A1 (en) * 2020-08-21 2022-02-24 Nuclera Nucleics Ltd Monitoring of in vitro protein synthesis
JP7761001B2 (ja) * 2020-09-10 2025-10-28 ニプロ株式会社 血液検査装置及び血液検査方法
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
WO2022066637A1 (en) * 2020-09-22 2022-03-31 Catalog Technologies, Inc. Temperature-controlled fluidic reactions system
TWI781660B (zh) * 2020-09-30 2022-10-21 富佳生技股份有限公司 核酸檢測盒及核酸檢測設備
JP2022058244A (ja) * 2020-09-30 2022-04-11 富佳生技股▲ふん▼有限公司 核酸検出箱及び核酸検出デバイス
WO2022074399A1 (en) 2020-10-08 2022-04-14 Nuclera Nucleics Ltd Electrowetting system and method for reagent-specific driving ewod arrays in microfluidic systems
KR20230091116A (ko) 2020-10-21 2023-06-22 일루미나, 인코포레이티드 다수의 삽입체를 포함하는 시퀀싱 주형, 및 시퀀싱 처리량을 개선하기 위한 조성물 및 방법
CN116635153A (zh) 2020-11-04 2023-08-22 核蛋白有限公司 用于数字微流体设备的电介质层
GB2602105A (en) * 2020-12-18 2022-06-22 Solentim Ltd Cell dispenser
US20220236249A1 (en) * 2021-01-25 2022-07-28 Koninklijke Philips N.V. Apparatus and method for sputum conditioning and analysis
EP4421491A3 (de) 2021-02-19 2024-11-27 10X Genomics, Inc. Verfahren zur verwendung einer modularen testträgervorrichtung
WO2023021295A2 (en) * 2021-08-18 2023-02-23 Nuclera Nucleics Ltd Methods and compositions for improved biomolecule assays on digital microfluidic devices
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
JP2024541917A (ja) * 2021-11-03 2024-11-13 アボット・ラボラトリーズ サンプル分析のためのシステム及び方法
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation
US20250060333A1 (en) 2023-08-16 2025-02-20 E Ink Corporation Devices, methods, and systems for visualizing electrowetting pathing using electrophoretic materials
NL2037264B1 (en) * 2024-03-15 2025-09-26 Digi Bio B V A microfluidic apparatus structured to perform a workflow assay procedure using an electrowetting on dielectric, EWOD, microfluidic device as well as such electrowetting on dielectric, EWOD, microfluidic device.

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6319668B1 (en) * 1995-04-25 2001-11-20 Discovery Partners International Method for tagging and screening molecules
US5851769A (en) * 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
US6379929B1 (en) * 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
FI980874A7 (fi) * 1998-04-20 1999-10-21 Wallac Oy Menetelmä ja laite pienten nestemäärien kemiallisen analyysin suorittamiseksi
US6226082B1 (en) * 1998-06-25 2001-05-01 Amira Medical Method and apparatus for the quantitative analysis of a liquid sample with surface enhanced spectroscopy
US20030012699A1 (en) * 1998-11-18 2003-01-16 Thomas Moore Simultaneous handling of magnetic beads in a two-dimensional arrangement
US6225061B1 (en) * 1999-03-10 2001-05-01 Sequenom, Inc. Systems and methods for performing reactions in an unsealed environment
US6432290B1 (en) * 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US6442413B1 (en) * 2000-05-15 2002-08-27 James H. Silver Implantable sensor
WO2002023163A1 (en) * 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
US6844028B2 (en) * 2001-06-26 2005-01-18 Accelr8 Technology Corporation Functional surface coating
US20030119057A1 (en) * 2001-12-20 2003-06-26 Board Of Regents Forming and modifying dielectrically-engineered microparticles
US6911132B2 (en) * 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7217542B2 (en) * 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
FR2846957B1 (fr) * 2002-11-13 2005-09-30 Commissariat Energie Atomique Micro-systeme a remplissage de micro-billes et procede d'obtention
US20040239080A1 (en) * 2003-05-30 2004-12-02 Berrahou Philip F. Housing retention mechanism
TWI230760B (en) * 2003-07-29 2005-04-11 Univ Tsinghua Electrowetting electrode design with electromagnetic field for actuation of the magnetic-beads biochemical detection system
US7767435B2 (en) * 2003-08-25 2010-08-03 University Of Washington Method and device for biochemical detection and analysis of subcellular compartments from a single cell
CA2479452C (en) * 2003-08-30 2008-11-04 F.Hoffmann-La Roche Ag Method and device for determining analytes in a liquid
KR100647282B1 (ko) * 2003-12-24 2006-11-17 삼성전자주식회사 ＤＮＡ 마이크로어레이 스폿팅(Ｓｐｏｔｉｎｇ) 장치 및이를 이용한 스폿팅 방법
WO2005069015A1 (ja) * 2004-01-15 2005-07-28 Japan Science And Technology Agency 化学分析装置及び化学分析方法
CA2553833C (en) * 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
KR100583231B1 (ko) * 2004-04-13 2006-05-26 한국과학기술연구원 물방울형 세포 부유액을 이용한 세포 분리 장치
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7300993B2 (en) * 2004-06-03 2007-11-27 Shell Oil Company Process for the preparation of polyether polyols
FR2871076A1 (fr) * 2004-06-04 2005-12-09 Univ Lille Sciences Tech Dispositif pour desorption par rayonnement laser incorporant une manipulation de l'echantillon liquide sous forme de gouttes individuelles permettant leur traitement chimique et biochimique
US7693666B2 (en) * 2004-07-07 2010-04-06 Rensselaer Polytechnic Institute Method, system, and program product for controlling chemical reactions in a digital microfluidic system
JP4047314B2 (ja) * 2004-09-07 2008-02-13 株式会社東芝 微細流路構造体

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