GB2555950A - Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays - Google Patents

Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays Download PDF

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Publication number
GB2555950A
GB2555950A GB1717171.1A GB201717171A GB2555950A GB 2555950 A GB2555950 A GB 2555950A GB 201717171 A GB201717171 A GB 201717171A GB 2555950 A GB2555950 A GB 2555950A
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United Kingdom
Prior art keywords
nucleic acid
addressable
signals
acid hybridization
array
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
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GB1717171.1A
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English (en)
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GB201717171D0 (en
Inventor
Hassibi Arjang
Jirage Kshama
Manickam Arun
Milaninia Kaveh
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Insilixa Inc
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Insilixa Inc
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Publication of GB201717171D0 publication Critical patent/GB201717171D0/en
Publication of GB2555950A publication Critical patent/GB2555950A/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Semiconductor Integrated Circuits (AREA)
GB1717171.1A 2015-03-23 2016-03-22 Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays Withdrawn GB2555950A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/665,904 US9708647B2 (en) 2015-03-23 2015-03-23 Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
PCT/US2016/023634 WO2016154227A1 (en) 2015-03-23 2016-03-22 Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays

Publications (2)

Publication Number Publication Date
GB201717171D0 GB201717171D0 (en) 2017-12-06
GB2555950A true GB2555950A (en) 2018-05-16

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ID=56976446

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1717171.1A Withdrawn GB2555950A (en) 2015-03-23 2016-03-22 Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays

Country Status (6)

Country Link
US (2) US9708647B2 (OSRAM)
EP (2) EP3912723A1 (OSRAM)
JP (3) JP7123560B2 (OSRAM)
CN (1) CN107614108B (OSRAM)
GB (1) GB2555950A (OSRAM)
WO (1) WO2016154227A1 (OSRAM)

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US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
CN107429281B (zh) 2014-12-31 2022-05-27 维斯比医学公司 用于分子诊断测试的装置和方法
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
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US10712308B2 (en) 2016-06-03 2020-07-14 International Business Machines Corporation Biosensor for electrical detection of a nucleotide sequence
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CN110997934B (zh) * 2017-05-31 2025-02-28 生捷科技控股公司 具有电子检测系统的寡核苷酸探针阵列
AU2018364741B2 (en) 2017-11-09 2021-03-25 Visby Medical, Inc. Portable molecular diagnostic device and methods for the detection of target viruses
JP2019093377A (ja) 2017-11-22 2019-06-20 株式会社エンプラス 流体チップ、流体デバイスおよびそれらの製造方法
MX2021002533A (es) 2018-09-03 2021-05-27 Visby Medical Inc Dispositivos y metodos para pruebas de susceptibilidad a antibioticos.
JP7687957B2 (ja) * 2019-03-14 2025-06-03 インシリクサ， インコーポレイテッド 時間ゲート蛍光ベースの検出のための方法およびシステム
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US11352675B2 (en) 2020-01-03 2022-06-07 Visby Medical, Inc. Devices and methods for antibiotic susceptability testing
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