BR0107831A - Ensaio homogêneo de hibridização duplex ou triplex por meio de múltiplas medições sob condições variadas - Google Patents

Ensaio homogêneo de hibridização duplex ou triplex por meio de múltiplas medições sob condições variadas

Info

Publication number
BR0107831A
BR0107831A BR0107831-3A BR0107831A BR0107831A BR 0107831 A BR0107831 A BR 0107831A BR 0107831 A BR0107831 A BR 0107831A BR 0107831 A BR0107831 A BR 0107831A
Authority
BR
Brazil
Prior art keywords
assay
hybridization
duplex
signals
homogeneous
Prior art date
Application number
BR0107831-3A
Other languages
English (en)
Inventor
Glen H Erikson
Pierre Picard
Jasmine I Daksis
Original Assignee
Ingeneus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingeneus Corp filed Critical Ingeneus Corp
Publication of BR0107831A publication Critical patent/BR0107831A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/113—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being electroactive, e.g. redox labels
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

"ENSAIO HOMOGêNEO DE HIBRIDIZAçãO DUPLEX OU TRIPLEX POR MEIO DE MúLTIPLAS MEDIçõES SOB CONDIçõES VARIADAS". A invenção apresenta métodos de ensaio homogêneos para hibridização de ácidos nucleicos, detecção e avaliação. O ensaio inclui obter sinais a partir de uma amostra de teste ambos antes como durante a aplicação de voltage à amostra de teste e correlacionar os sinais, cada um dos quais é indicativo da afinidade de ligação da sonda e do alvo de cada um. O ensaio permite determinar uma extensão de correspondência entre a sonda e o alvo, a medida que a voltage pode ser calibrada de maneira a desestabilizar de forma significativa qualquer hibridização, exceto hibridização perfeitamente complementar. Os sinais cuja magnitude está correlacionada com a afinidade de ligação pode ser condutância elétrica e/ou intensidade de fluorescência. Preferivelmente, ambos os pares de sinais são medidos e comparados de maneira a aumentar a confiabilidade do ensaio. O ensaio pode detectar hibridização específica entre sondas de fita única e alvos de fita dupla não desnaturados para formar triplexes, evitando assim a necessidade de desnaturar os alvos. Os métodos de ensaio podem também ser aplicados a complexos de hibridização duplex.
BR0107831-3A 2000-01-24 2001-01-23 Ensaio homogêneo de hibridização duplex ou triplex por meio de múltiplas medições sob condições variadas BR0107831A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/490,273 US6265170B1 (en) 2000-01-24 2000-01-24 Homogenous assay of duplex of triplex hybridization by means of multiple measurements under varied conditions
PCT/IB2001/000077 WO2001053526A2 (en) 2000-01-24 2001-01-23 Homogenous assay of duplex or triplex hybridization by means of multiple measurements under varied conditions

Publications (1)

Publication Number Publication Date
BR0107831A true BR0107831A (pt) 2004-07-27

Family

ID=23947351

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0107831-3A BR0107831A (pt) 2000-01-24 2001-01-23 Ensaio homogêneo de hibridização duplex ou triplex por meio de múltiplas medições sob condições variadas

Country Status (15)

Country Link
US (1) US6265170B1 (pt)
EP (1) EP1250459B1 (pt)
JP (1) JP4182149B2 (pt)
KR (1) KR100488185B1 (pt)
CN (1) CN1253583C (pt)
AT (1) ATE408709T1 (pt)
AU (1) AU784178B2 (pt)
BR (1) BR0107831A (pt)
CA (1) CA2398362C (pt)
DE (1) DE60135827D1 (pt)
HK (1) HK1047455A1 (pt)
IL (2) IL150844A0 (pt)
MX (1) MXPA02007210A (pt)
RU (1) RU2242518C2 (pt)
WO (1) WO2001053526A2 (pt)

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US6403313B1 (en) 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
US6656692B2 (en) * 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US20030181412A1 (en) * 1999-12-21 2003-09-25 Ingeneus Corporation Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses
US6924108B2 (en) 1999-12-21 2005-08-02 Ingeneus Corporation Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
US7309569B2 (en) 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US6927027B2 (en) 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
US6613524B1 (en) * 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
US6982147B2 (en) * 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US7220541B2 (en) * 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
DE10155053B4 (de) * 2001-11-09 2005-11-24 Friz Biochem Gesellschaft Für Bioanalytik Mbh Reversible Bindung eines Fluorophors an eine Oberfläche zur Detektion von Ligat-Ligand-Assoziationsereignissen durch Fluoreszenz-Quenchen
DE10155031A1 (de) * 2001-11-09 2003-05-28 Friz Biochem Gmbh Fluoreszenz-Quenchen zur Detektion von Ligat-Ligand Assoziationsereignissen in elektrischen Feldern
JP2005289810A (ja) * 2002-03-18 2005-10-20 Sangaku Renkei Kiko Kyushu:Kk 蛍光色素基およびインターカレート基を含有する二本鎖核酸を選択的に検出するための蛍光試薬
US20040142329A1 (en) * 2003-01-17 2004-07-22 Ingeneus Corporation Probe conjugation to increase multiplex binding motif preference
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
KR100994752B1 (ko) 2003-03-31 2010-11-16 디지탈 지노믹스(주) 디엔에이 하이브리드형성의 전기적 검출방법과 이를 위한디엔에이칩 및 그 제조방법
GB2422196B (en) 2003-05-20 2008-05-07 Investigen Inc System for detecting polynucleotides
AU2005286084C1 (en) * 2004-09-24 2011-11-24 Ingeneus Inc. Genomic assay
WO2007100711A2 (en) 2006-02-24 2007-09-07 Investigen, Inc. Methods and compositions for detecting polynucleotides
EP2100138B1 (en) * 2006-12-28 2012-06-20 Wako Pure Chemical Industries, Ltd. Method for internal standardization of assays
GB0701444D0 (en) * 2007-01-25 2007-03-07 Iti Scotland Ltd Detecting analytes
WO2008103702A2 (en) * 2007-02-23 2008-08-28 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
EP2172563A1 (en) * 2008-09-24 2010-04-07 bioMérieux S.A. Method for lowering the dependency towards sequence variation of a nucleic acid target in a diagnostic hybridization assay
MX340258B (es) * 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
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Also Published As

Publication number Publication date
KR20020065940A (ko) 2002-08-14
KR100488185B1 (en) 2005-05-09
IL150844A0 (en) 2003-02-12
JP2003523738A (ja) 2003-08-12
RU2002122770A (ru) 2004-03-10
WO2001053526A2 (en) 2001-07-26
ATE408709T1 (de) 2008-10-15
WO2001053526A3 (en) 2002-06-13
MXPA02007210A (es) 2002-12-09
JP4182149B2 (ja) 2008-11-19
DE60135827D1 (de) 2008-10-30
CA2398362C (en) 2010-05-04
RU2242518C2 (ru) 2004-12-20
CN1253583C (zh) 2006-04-26
AU3042501A (en) 2001-07-31
HK1047455A1 (zh) 2003-02-21
EP1250459A2 (en) 2002-10-23
CN1447861A (zh) 2003-10-08
IL150844A (en) 2008-04-13
CA2398362A1 (en) 2001-07-26
US6265170B1 (en) 2001-07-24
EP1250459B1 (en) 2008-09-17
AU784178B2 (en) 2006-02-16

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O COMBINADO COM 11 E ART. 8O COMBINADO COM O ART 13 DA LPI

B12B Appeal against refusal [chapter 12.2 patent gazette]
B11D Dismissal acc. art. 38, par 2 of ipl - failure to pay fee after grant in time