WO2004096986A3 - Method for quantitative detection of nucleic acid molecules - Google Patents

Method for quantitative detection of nucleic acid molecules Download PDF

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Publication number
WO2004096986A3
WO2004096986A3 PCT/US2004/012161 US2004012161W WO2004096986A3 WO 2004096986 A3 WO2004096986 A3 WO 2004096986A3 US 2004012161 W US2004012161 W US 2004012161W WO 2004096986 A3 WO2004096986 A3 WO 2004096986A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
target nucleic
capture probes
sample
Prior art date
Application number
PCT/US2004/012161
Other languages
French (fr)
Other versions
WO2004096986A2 (en
Inventor
Dennis M Connolly
Charles D Deboer
Original Assignee
Integrated Nano Tech Llc
Dennis M Connolly
Charles D Deboer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated Nano Tech Llc, Dennis M Connolly, Charles D Deboer filed Critical Integrated Nano Tech Llc
Publication of WO2004096986A2 publication Critical patent/WO2004096986A2/en
Publication of WO2004096986A3 publication Critical patent/WO2004096986A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Abstract

The present invention is directed to a method of quantitatively detecting target nucleic acid molecules in a sample. In carrying out this method, one or more different groups of two or more electrically separated electrical conductors are provided with capture probes attached to the conductors such that a gap exists between the capture probes on the electrically separated conductors. The capture probes are contacted with a sample, potentially containing the target nucleic acid molecules, under conditions effective to permit any of the target nucleic acid molecule present in the sample to hybridize to the capture probes and thereby connect the capture probes. The presence of the target nucleic acid molecules is detected by determining whether electricity is conducted between the electrically separated conductors. The electrically separated conductors are then interrogated to quantify the concentration of target nucleic acid molecules present in the sample.
PCT/US2004/012161 2003-04-29 2004-04-20 Method for quantitative detection of nucleic acid molecules WO2004096986A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46653603P 2003-04-29 2003-04-29
US60/466,536 2003-04-29

Publications (2)

Publication Number Publication Date
WO2004096986A2 WO2004096986A2 (en) 2004-11-11
WO2004096986A3 true WO2004096986A3 (en) 2005-03-24

Family

ID=33418393

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/012161 WO2004096986A2 (en) 2003-04-29 2004-04-20 Method for quantitative detection of nucleic acid molecules

Country Status (1)

Country Link
WO (1) WO2004096986A2 (en)

Families Citing this family (15)

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Publication number Priority date Publication date Assignee Title
AT505495A1 (en) * 2007-07-04 2009-01-15 Arc Austrian Res Centers Gmbh METHOD FOR IDENTIFYING AND QUANTIFYING ORGANIC AND BIOCHEMICAL SUBSTANCES
PT2504105T (en) 2009-11-24 2021-03-31 Opko Diagnostics Llc Fluid mixing and delivery in microfluidic systems
CN109071212A (en) 2016-01-28 2018-12-21 罗斯韦尔生物技术股份有限公司 Use the method and apparatus of large-scale molecular electronic sensor array measurement analyte
US10712334B2 (en) 2016-01-28 2020-07-14 Roswell Biotechnologies, Inc. Massively parallel DNA sequencing apparatus
WO2017139493A2 (en) 2016-02-09 2017-08-17 Roswell Biotechnologies, Inc. Electronic label-free dna and genome sequencing
US10597767B2 (en) 2016-02-22 2020-03-24 Roswell Biotechnologies, Inc. Nanoparticle fabrication
US9829456B1 (en) 2016-07-26 2017-11-28 Roswell Biotechnologies, Inc. Method of making a multi-electrode structure usable in molecular sensing devices
US10902939B2 (en) 2017-01-10 2021-01-26 Roswell Biotechnologies, Inc. Methods and systems for DNA data storage
KR20230158636A (en) 2017-01-19 2023-11-20 로스웰 바이오테크놀로지스 인코포레이티드 Solid state sequencing devices comprising two dimensional layer materials
US10508296B2 (en) 2017-04-25 2019-12-17 Roswell Biotechnologies, Inc. Enzymatic circuits for molecular sensors
CA3057151A1 (en) 2017-04-25 2018-11-01 Roswell Biotechnologies, Inc. Enzymatic circuits for molecular sensors
EP4023764A3 (en) 2017-05-09 2022-09-21 Roswell Biotechnologies, Inc. Binding probe circuits for molecular sensors
KR20200039795A (en) 2017-08-30 2020-04-16 로스웰 바이오테크놀로지스 인코포레이티드 Progressive enzyme molecular electronic sensors for DNA data storage
EP3694990A4 (en) 2017-10-10 2022-06-15 Roswell Biotechnologies, Inc. Methods, apparatus and systems for amplification-free dna data storage
CN112805391A (en) * 2018-08-14 2021-05-14 高丽大学校产学协力团 Method for synthesizing single metal nanobridge structure and method for manufacturing DNA point mutation detection sensor by using the same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000060125A2 (en) * 1999-04-07 2000-10-12 Dennis Michael Connolly High resolution dna detection methods and devices

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000060125A2 (en) * 1999-04-07 2000-10-12 Dennis Michael Connolly High resolution dna detection methods and devices
US6399303B1 (en) * 1999-04-07 2002-06-04 Integrated Nano-Technologies, Llc High resolution DNA detection methods and devices

Also Published As

Publication number Publication date
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