GB2499340B - Methods for sequencing nucleic acids - Google Patents

Methods for sequencing nucleic acids

Info

Publication number
GB2499340B
GB2499340B GB1308138.5A GB201308138A GB2499340B GB 2499340 B GB2499340 B GB 2499340B GB 201308138 A GB201308138 A GB 201308138A GB 2499340 B GB2499340 B GB 2499340B
Authority
GB
United Kingdom
Prior art keywords
methods
nucleic acids
sequencing nucleic
sequencing
acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB1308138.5A
Other languages
English (en)
Other versions
GB2499340A (en
GB201308138D0 (en
Inventor
Hesaam Esfandyarpour
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genapsys Inc
Original Assignee
Genapsys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genapsys Inc filed Critical Genapsys Inc
Publication of GB201308138D0 publication Critical patent/GB201308138D0/en
Publication of GB2499340A publication Critical patent/GB2499340A/en
Application granted granted Critical
Publication of GB2499340B publication Critical patent/GB2499340B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/286—Magnetic plugs and dipsticks disposed at the inner circumference of a recipient, e.g. magnetic drain bolt
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/30—Combinations with other devices, not otherwise provided for
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10D—INORGANIC ELECTRIC SEMICONDUCTOR DEVICES
    • H10D99/00—Subject matter not provided for in other groups of this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/10—Apparatus specially adapted for use in combinatorial chemistry or with libraries for identifying library members
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N2001/4038—Concentrating samples electric methods, e.g. electromigration, electrophoresis, ionisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Electrochemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Fluid Mechanics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Ceramic Engineering (AREA)
GB1308138.5A 2010-10-04 2011-10-04 Methods for sequencing nucleic acids Expired - Fee Related GB2499340B (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US38948410P 2010-10-04 2010-10-04
US38949010P 2010-10-04 2010-10-04
US201161443167P 2011-02-15 2011-02-15
US201161491081P 2011-05-27 2011-05-27
PCT/US2011/054769 WO2012047889A2 (en) 2010-10-04 2011-10-04 Systems and methods for automated reusable parallel biological reactions

Publications (3)

Publication Number Publication Date
GB201308138D0 GB201308138D0 (en) 2013-06-12
GB2499340A GB2499340A (en) 2013-08-14
GB2499340B true GB2499340B (en) 2015-10-28

Family

ID=45928377

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1308138.5A Expired - Fee Related GB2499340B (en) 2010-10-04 2011-10-04 Methods for sequencing nucleic acids

Country Status (12)

Country Link
US (7) US9187783B2 (cg-RX-API-DMAC10.html)
EP (1) EP2625526B1 (cg-RX-API-DMAC10.html)
JP (1) JP6114694B2 (cg-RX-API-DMAC10.html)
KR (1) KR101963462B1 (cg-RX-API-DMAC10.html)
CN (1) CN103328981B (cg-RX-API-DMAC10.html)
AU (1) AU2011312218B2 (cg-RX-API-DMAC10.html)
GB (1) GB2499340B (cg-RX-API-DMAC10.html)
IL (1) IL225558B (cg-RX-API-DMAC10.html)
MX (1) MX344363B (cg-RX-API-DMAC10.html)
NZ (1) NZ610129A (cg-RX-API-DMAC10.html)
SG (1) SG189839A1 (cg-RX-API-DMAC10.html)
WO (1) WO2012047889A2 (cg-RX-API-DMAC10.html)

Families Citing this family (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2625526B1 (en) 2010-10-04 2017-03-15 Genapsys Inc. Systems and methods for automated reusable parallel biological reactions
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
KR101940833B1 (ko) 2011-05-27 2019-01-21 제납시스 인크. 유전자 및 생물학적 분석을 위한 시스템 및 방법
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
CN104105797B (zh) 2011-12-01 2016-08-31 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
WO2013119845A1 (en) 2012-02-07 2013-08-15 Vibrant Holdings, Llc Substrates, peptide arrays, and methods
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10006909B2 (en) 2012-09-28 2018-06-26 Vibrant Holdings, Llc Methods, systems, and arrays for biomolecular analysis
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP6082605B2 (ja) * 2013-01-30 2017-02-15 株式会社日立ハイテクノロジーズ 分析装置
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
US10934895B2 (en) 2013-03-04 2021-03-02 Echogen Power Systems, Llc Heat engine systems with high net power supercritical carbon dioxide circuits
EP2971141B1 (en) * 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
US9591268B2 (en) * 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
CN103540672B (zh) * 2013-10-29 2015-04-08 中国科学技术大学 一种亲和核酸分子的快速鉴定和分离方法
WO2015077632A1 (en) 2013-11-21 2015-05-28 Avails Medical, Inc. Electrical biosensor for detecting a substance in a bodily fluid, and method and system for same
EP3080300B1 (en) 2013-12-11 2020-09-02 Genapsys Inc. Systems and methods for biological analysis and computation
US11318479B2 (en) 2013-12-18 2022-05-03 Berkeley Lights, Inc. Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
EP3110965B1 (en) * 2014-02-25 2019-01-16 Roche Diagnostics GmbH Method of identifying a nucleotide at a defined position and determining the sequence of a target polynucleotide using an electro-switchable biosensor
EP3117221B1 (en) 2014-03-13 2020-09-09 Genapsys Inc. Microfluidic devices and methods for sample preparation and analysis
CA2942822A1 (en) 2014-03-31 2015-10-08 Multerra Bio, Inc. Low-cost packaging for fluidic and device co-integration
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
EP3132060B1 (en) 2014-04-18 2019-03-13 Genapsys Inc. Methods and systems for nucleic acid amplification
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3161161A4 (en) * 2014-06-26 2018-02-28 10X Genomics, Inc. Methods and compositions for sample analysis
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
CN107075433A (zh) * 2014-09-22 2017-08-18 东芝医疗系统株式会社 核酸检查装置
JP2017532042A (ja) 2014-10-29 2017-11-02 10エックス ゲノミクス,インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
KR102303439B1 (ko) * 2014-11-12 2021-09-24 한국전기연구원 면역자기장분리용 바이오 칩 및 이의 제조방법
US9702847B2 (en) 2014-12-30 2017-07-11 Avails Medical, Inc. Systems and methods for detecting a substance in bodily fluid
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP3230721B1 (en) * 2015-01-30 2020-06-03 Hewlett-Packard Development Company, L.P. Diagnostic chip
WO2016125251A1 (ja) * 2015-02-03 2016-08-11 株式会社日立製作所 単一細胞解析用フローセルデバイス及び単一細胞解析装置
WO2016127077A2 (en) 2015-02-06 2016-08-11 Genapsys, Inc Systems and methods for detection and analysis of biological species
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
WO2016160475A1 (en) 2015-03-30 2016-10-06 Genapsys, Inc. Beads for nucleic acid sequencing
EP3295163B1 (en) * 2015-05-12 2020-12-02 Magarray, Inc. Devices and methods for increasing magnetic sensor sensitivity
WO2016207320A1 (en) 2015-06-26 2016-12-29 Universiteit Twente Microfluidic device and method for batch adsorption
GB2543474A (en) * 2015-07-02 2017-04-26 Univ College Dublin Nat Univ Of Ireland Dublin An optical detection based on non-linear magnetophoretic transport of magnetic particle for particle and biological sensing and separation
WO2017035393A1 (en) 2015-08-25 2017-03-02 eSense, LLC Devices, systems and methods for detecting viable microorganisms in a fluid sample
KR20180085783A (ko) 2015-11-23 2018-07-27 버클리 라잇츠, 인크. 인 시츄-생성된 미세유체 격리 구조체들, 키트들, 및 그 이용 방법들
KR102723865B1 (ko) 2015-12-04 2024-10-29 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
IL259837B2 (en) 2015-12-08 2023-03-01 Berkeley Lights Inc Microfluidic devices and kits and their uses
JP7109785B2 (ja) 2016-01-14 2022-08-01 ロズウェル バイオテクノロジーズ,インコーポレイテッド 分子センサーおよび関連方法
EP3356511B1 (en) 2016-01-25 2022-04-27 Avails Medical, Inc. Methods for detecting viable infectious agents in a fluid sample using an electrolyte-insulator-semiconductor sensor
EP3408220A4 (en) 2016-01-28 2019-09-04 Roswell Biotechnologies, Inc METHOD AND DEVICE FOR MEASURING ANALYTES USING LARGE CALCULAR MOLECULAR ELECTRONIC SENSOR ARRAYS
US10712334B2 (en) 2016-01-28 2020-07-14 Roswell Biotechnologies, Inc. Massively parallel DNA sequencing apparatus
US10737263B2 (en) 2016-02-09 2020-08-11 Roswell Biotechnologies, Inc. Electronic label-free DNA and genome sequencing
US10597767B2 (en) 2016-02-22 2020-03-24 Roswell Biotechnologies, Inc. Nanoparticle fabrication
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2017209839A1 (en) 2016-05-31 2017-12-07 Avails Medical, Inc. Detecting viable infectious agents in a fluid sample and susceptibility of infectious agents to anti-infectives
EP3488017A4 (en) 2016-07-20 2020-02-26 Genapsys Inc. SYSTEMS AND METHODS FOR SEQUENCING NUCLEIC ACIDS
US9829456B1 (en) 2016-07-26 2017-11-28 Roswell Biotechnologies, Inc. Method of making a multi-electrode structure usable in molecular sensing devices
JP6759881B2 (ja) * 2016-09-05 2020-09-23 アイシン精機株式会社 生体分子検出装置および生体分子検出装置の製造方法
CN106404863B (zh) * 2016-11-04 2018-11-23 北京农业信息技术研究中心 一种活体在线检测植物玉米素的微电极生物传感器及其应用
DE102016222032A1 (de) 2016-11-10 2018-05-17 Robert Bosch Gmbh Mikrofluidische Vorrichtung und Verfahren zur Analyse von Nukleinsäuren
WO2018111234A1 (en) 2016-12-13 2018-06-21 Avails Medical, Inc. DEVICES, SYSTEMS AND METHODS TO DETECT THE PRESENCE OF ß-LACTAM ANTIBIOTIC HYDROLYZING BACTERIA IN A SAMPLE
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN110431148A (zh) 2017-01-10 2019-11-08 罗斯威尔生命技术公司 用于dna数据存储的方法和系统
KR102685937B1 (ko) 2017-01-19 2024-07-17 로스웰 엠이 아이엔씨. 2차원 레이어 재료를 포함하는 솔리드 스테이트 시퀀싱 디바이스들
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
KR102692957B1 (ko) 2017-04-25 2024-08-06 로스웰 엠이 아이엔씨. 분자 센서들을 위한 효소 회로들
CN110651182B (zh) 2017-05-09 2022-12-30 罗斯威尔生命技术公司 用于分子传感器的结合探针电路
CN107118955B (zh) 2017-05-12 2020-03-10 京东方科技集团股份有限公司 基因测序芯片及基因测序方法
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018218250A2 (en) 2017-05-26 2018-11-29 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
WO2019005296A1 (en) 2017-06-27 2019-01-03 Avails Medical, Inc. APPARATUS, SYSTEMS AND METHODS FOR DETERMINING THE SENSITIVITY OF MICROORGANISMS TO ANTI-INFECTIOUS
EP3676389A4 (en) 2017-08-30 2021-06-02 Roswell Biotechnologies, Inc PROCESSIVE ENZYMATIC MOLECULAR ELECTRONIC SENSORS FOR STORING DNA DATA
EP3684951A4 (en) 2017-09-21 2021-06-16 Genapsys, Inc. NUCLEIC ACID SEQUENCING SYSTEMS AND METHODS
WO2019070739A1 (en) 2017-10-03 2019-04-11 Avails Medical, Inc. APPARATUSES, SYSTEMS AND METHODS FOR DETERMINING THE CONCENTRATION OF MICROORGANISMS AND THE SENSITIVITY OF MICROORGANISMS TO ANTI-INFECTIOUS, BASED ON OXIDOREDUCTION REACTIONS
WO2019075100A1 (en) 2017-10-10 2019-04-18 Roswell Biotechnologies, Inc. METHODS, APPARATUS AND SYSTEMS FOR STORING DNA DATA WITHOUT AMPLIFICATION
US12226745B2 (en) 2017-11-07 2025-02-18 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
WO2019094524A1 (en) * 2017-11-07 2019-05-16 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
WO2019099908A1 (en) 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
JP7254077B2 (ja) 2017-12-05 2023-04-07 アベイルズ メディカル,インコーポレイテッド 下流試験用の規定濃度の感染因子を含む出力サンプルの調製
EP3731959B1 (en) 2017-12-29 2025-10-08 Clear Labs, Inc. Automated priming and library loading device
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
CA3067181A1 (en) 2018-04-20 2019-10-24 Illumina, Inc. Methods of encapsulating single cells, the encapsulated cells and uses thereof
TWI714069B (zh) * 2018-05-04 2020-12-21 美商伊路米納有限公司 具有集成歧管的流動池
EP3790985B1 (en) 2018-05-09 2026-04-15 Vibrant Holdings, LLC Methods of synthesizing a polynucleotide array using photactivated agents
CN121472373A (zh) 2018-05-22 2026-02-06 安序源有限公司 用于核酸测序的方法、系统和组合物
CN113195085B (zh) 2018-10-11 2024-04-19 伯克利之光生命科技公司 用于识别优化蛋白产生的系统和方法以及其试剂盒
MY202795A (en) * 2018-10-26 2024-05-22 Illumina Inc Modulating polymer beads for dna processing
WO2020117650A1 (en) 2018-12-03 2020-06-11 Avails Medical, Inc. Apparatus, systems, and methods for quantifying infectious agents
TWI725686B (zh) 2018-12-26 2021-04-21 財團法人工業技術研究院 用於產生液珠的管狀結構及液珠產生方法
CN111450906B (zh) * 2019-01-22 2022-02-25 北京纳米能源与系统研究所 自驱动型电润湿阀门、纸基微流体芯片及免疫检测器件
CA3129315A1 (en) 2019-02-11 2020-08-20 Ultima Genomics, Inc. Methods for nucleic acid analysis
US12504396B2 (en) 2019-04-12 2025-12-23 SemiconBio Polycyclic aromatic bridges for molecular electronic sensors
CN110286120B (zh) * 2019-08-15 2024-04-02 北京市农林科学院 流动式电化学发光生物检测系统
US11814675B2 (en) * 2019-08-29 2023-11-14 Robert Bosch Gmbh Edge sequencing with an immobilized translocator
WO2021045900A1 (en) 2019-09-06 2021-03-11 Roswell Biotechnologies, Inc. Methods of fabricating nanoscale structures usable in molecular sensors and other devices
WO2021092524A1 (en) 2019-11-07 2021-05-14 Smylio Inc. Saliva collection and testing system
CN120502365A (zh) * 2020-04-21 2025-08-19 赫孚孟拉罗股份公司 具有单分子传感器阵列的高通量核酸定序
MA61232A1 (fr) 2020-12-09 2024-05-31 Supercritical Storage Company Inc Système de stockage d'énergie thermique électrique à trois réservoirs
WO2022236225A2 (en) * 2021-04-23 2022-11-10 The General Hospital Corporation Fluidic transistors and uses thereof
EP4337791A1 (en) * 2021-05-10 2024-03-20 Trustees of Boston University Printed biogel nanosensors
WO2023055752A2 (en) 2021-09-28 2023-04-06 Axbio Inc. Methods for processing a nucleic acid sample and compositions thereof
US12669417B2 (en) 2022-03-28 2026-06-30 Bruker Spatial Biology, Inc. Method for assaying a micro-object within a microfluidic device
CN115747302B (zh) * 2022-11-25 2026-06-30 常州大学 基于气泡界面振动的dna片段化方法及装置
WO2025128699A1 (en) * 2023-12-12 2025-06-19 Illumina, Inc. Capturing and amplifying polynucleotides using molecules and particles
CN117905529B (zh) * 2024-03-19 2024-05-24 山西中科智谷科技有限公司 一种煤矿安全监测装置及监测方法

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001018246A1 (en) * 1999-08-26 2001-03-15 The Trustees Of Princeton University Microfluidic and nanofluidic electronic devices for detecting changes in capacitance of fluids and methods of using
WO2001042508A2 (en) * 1999-12-09 2001-06-14 Motorola, Inc. Methods and compositions relating to electrical detection of nucleic acid reactions
US20030209432A1 (en) * 2000-12-11 2003-11-13 Choong Vi-En Methods and compositions relating to electrical detection of nucleic acid reactions
US20050019784A1 (en) * 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
WO2005008450A2 (en) * 2003-03-28 2005-01-27 The Regents Of The University Of California Method and apparatus for nanogap device and array
WO2005121363A2 (en) * 2004-06-11 2005-12-22 Medical Biosystems Limited Method for determining biophysical properties
US20060008824A1 (en) * 2004-05-20 2006-01-12 Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
US20070184463A1 (en) * 2005-09-30 2007-08-09 Caliper Life Sciences, Inc. Microfluidic device for purifying a biological component using magnetic beads
WO2008108612A1 (es) * 2007-03-06 2008-09-12 Salinas Garcia Jose Marco Pieza para la fijación y el aislamiento de conductores eléctricos
US20080241841A1 (en) * 2007-03-30 2008-10-02 Hitachi, Ltd. Method and apparatus for sample preparation
WO2009012112A1 (en) * 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
US20100078325A1 (en) * 2008-09-03 2010-04-01 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US20100112588A1 (en) * 2008-11-04 2010-05-06 Caerus Molecular Diagnostics, Inc. Methods for sanger sequencing using particle associated clonal amplicons and highly parallel electrophoretic size-based separation

Family Cites Families (205)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2014761A (en) 1929-12-05 1935-09-17 Waldhof Zellstoff Fab Process for the manufacture of solutions from cellulose
SE407115B (sv) * 1975-10-06 1979-03-12 Kabi Ab Forfarande och metelektroder for studium av enzymatiska och andra biokemiska reaktioner
US4959615A (en) 1988-05-31 1990-09-25 Micro Encoder, Inc. Electrode structure for capacitance-type measurement transducers
US5612181A (en) 1989-06-21 1997-03-18 Fourmentin-Guilbert; Jean E. R. Method for arranging a polynucleotide on a substrate for point-by-point analysis of the bases thereof
US5407799A (en) 1989-09-14 1995-04-18 Associated Universities, Inc. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides
JP3025027B2 (ja) 1991-01-21 2000-03-27 株式会社日立製作所 酸素センサ
CA2121797A1 (en) 1991-10-21 1993-04-29 James W. Holm-Kennedy Method and device for biochemical sensing
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5602042A (en) 1994-04-14 1997-02-11 Cytyc Corporation Method and apparatus for magnetically separating biological particles from a mixture
CA2189958C (en) 1994-05-11 2005-04-19 Adrian Parton Methods of capturing species from liquids and assay procedures
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
ATE508200T1 (de) 1999-02-23 2011-05-15 Caliper Life Sciences Inc Sequenzierung durch inkorporation
CN1181337C (zh) * 2000-08-08 2004-12-22 清华大学 微流体系统中实体分子的操纵方法及相关试剂盒
US6046097A (en) 1999-03-23 2000-04-04 United Microelectronics Corp. Deposition method with improved step coverage
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6372813B1 (en) 1999-06-25 2002-04-16 Motorola Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
AU4504001A (en) 1999-11-04 2001-06-04 Princeton University Electrodeless dielectrophoresis for polarizable particles
US6616819B1 (en) 1999-11-04 2003-09-09 Therasense, Inc. Small volume in vitro analyte sensor and methods
CA2392009C (en) 1999-11-17 2009-07-14 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
US7682837B2 (en) 2000-05-05 2010-03-23 Board Of Trustees Of Leland Stanford Junior University Devices and methods to form a randomly ordered array of magnetic beads and uses thereof
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
EP1320922A2 (en) 2000-09-27 2003-06-25 Aviva Biosciences Corporation Apparatus for switchng and manipulating particles and method of use thereof
EP3034627B1 (en) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massive parallel method for decoding dna and rna
CN1481441A (zh) 2000-10-20 2004-03-10 斯坦福大学受托管理委员会 根据瞬时电信号表征样品中分子间相互作用和运动的方法和设备
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
WO2002061146A1 (en) * 2001-02-01 2002-08-08 The Trustees Of Columbia University In The City Of New York Use of electrical fields to control interactions between proteins and nucleic acid constructions immobilized on solid supports
GB0105831D0 (en) 2001-03-09 2001-04-25 Toumaz Technology Ltd Method for dna sequencing utilising enzyme linked field effect transistors
US8114591B2 (en) 2001-03-09 2012-02-14 Dna Electronics Ltd. Sensing apparatus and method
US20040023253A1 (en) 2001-06-11 2004-02-05 Sandeep Kunwar Device structure for closely spaced electrodes
WO2003004690A2 (en) 2001-07-06 2003-01-16 454$m(3) CORPORATION Method for isolation of independent, parallel chemical micro-reactions using a porous filter
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US8152991B2 (en) 2005-10-27 2012-04-10 Nanomix, Inc. Ammonia nanosensors, and environmental control system
US8154093B2 (en) 2002-01-16 2012-04-10 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
WO2005118853A2 (en) 2004-06-01 2005-12-15 Asm Scientific, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
DK1499738T3 (da) 2002-02-21 2008-11-17 Asm Scient Inc Recombinase-polymerease-amplifikation
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US6953958B2 (en) 2002-03-19 2005-10-11 Cornell Research Foundation, Inc. Electronic gain cell based charge sensor
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
DE10221799A1 (de) 2002-05-15 2003-11-27 Fujitsu Ltd Silicon-on-Insulator-Biosensor
GB0211564D0 (en) 2002-05-21 2002-06-26 Tournaz Technology Ltd Reference circuit
WO2003101736A2 (en) 2002-05-29 2003-12-11 Arizona Board Of Regents, Acting On Behalf Of Arizona State University Nanoscale ink-jet printing
WO2004003535A1 (en) 2002-06-27 2004-01-08 Nanosys Inc. Planar nanowire based sensor elements, devices, systems and methods for using and making same
KR100479128B1 (ko) 2002-07-22 2005-03-28 학교법인 한양학원 디앤에이 교배 검출을 위한 자기변형 바이오센서 및 그 제조방법
TWI234584B (en) * 2002-08-16 2005-06-21 Univ Nat Taiwan Normal Nucleic acid sequencing method
US20040197793A1 (en) 2002-08-30 2004-10-07 Arjang Hassibi Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
US7095010B2 (en) 2002-12-04 2006-08-22 California Institute Of Technology Silicon on insulator resonator sensors and modulators and method of operating the same
US7932025B2 (en) 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
WO2004060044A2 (en) 2003-01-02 2004-07-22 Bioforce Nanosciences, Inc. Method and apparatus for molecular analysis in small sample volumes
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
CN1791682B (zh) 2003-02-26 2013-05-22 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US20050123937A1 (en) 2003-03-07 2005-06-09 Thorp H. H. Methods for the electrochemical detection of target compounds
DE10319045A1 (de) 2003-04-25 2004-12-09 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Vorrichtung und Verfahren zur Aufbereitung Biopolymerhaltiger Flüssigkeiten
WO2004106891A2 (en) 2003-05-22 2004-12-09 University Of Hawaii Ultrasensitive biochemical sensor
PT1633888E (pt) 2003-06-17 2009-04-22 Keygene Nv Meios e método para a detecção de sequências de nucleótidos alvo utilizando ensaios de ligação com pares de sondas de oligonucleótido melhoradas
AU2004253882B2 (en) 2003-06-20 2010-06-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005019798A2 (en) 2003-08-13 2005-03-03 The Regents Of The University Of Michigan Biochemical sensors with micro-resonators
US20050084980A1 (en) 2003-10-17 2005-04-21 Intel Corporation Method and device for detecting a small number of molecules using surface-enhanced coherant anti-stokes raman spectroscopy
US20100000881A1 (en) 2003-10-30 2010-01-07 North Carolina State University Electrochemical detection of nucleic acid hybridization
WO2005043160A2 (en) 2003-10-31 2005-05-12 University Of Hawaii Ultrasensitive biochemical sensing platform
US7294246B2 (en) 2003-11-06 2007-11-13 3M Innovative Properties Company Electrode for electrochemical sensors
WO2005108612A2 (en) 2003-11-28 2005-11-17 Genorx, Inc. Nanoscale biosensor device, system and technique
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US20050129526A1 (en) 2003-12-10 2005-06-16 Dukhin Andrei S. Method of using unbalanced alternating electric field in microfluidic devices
JP3903183B2 (ja) 2004-02-03 2007-04-11 独立行政法人物質・材料研究機構 遺伝子検出電界効果デバイスおよびこれを用いた遺伝子多型解析方法
US20050218464A1 (en) 2004-03-18 2005-10-06 Holm-Kennedy James W Biochemical ultrasensitive charge sensing
US7238536B1 (en) 2004-03-22 2007-07-03 Florida State University Research Foundation, Inc. Controlled transport through multiple reversible interaction point membranes
AU2005254984B2 (en) 2004-06-10 2008-10-16 Ge Healthcare Bio-Sciences Corp. Method for nucleic acid analysis
US8536661B1 (en) 2004-06-25 2013-09-17 University Of Hawaii Biosensor chip sensor protection methods
GB2416210B (en) 2004-07-13 2008-02-20 Christofer Toumazou Ion sensitive field effect transistors
WO2006022370A1 (ja) 2004-08-27 2006-03-02 National Institute For Materials Science 電界効果デバイスを用いたdna塩基配列解析方法及び塩基配列解析装置
EP1788383B1 (en) 2004-08-30 2017-04-05 Japan Science and Technology Agency Dna sensor
EP1812604A4 (en) 2004-11-03 2011-02-23 Third Wave Tech Inc DETECTION ASSAY IN ONE STEP
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
EP1831670A4 (en) 2004-12-28 2010-09-15 Nanomix Inc NANOELECTRONIC DEVICES FOR DNA DETECTION AND DETECTION OF POLYNUCLEOTIDE SEQUENCES
EP1835916B1 (en) 2004-12-30 2009-04-01 Medivir Ab Compounds useful in the treatment of hiv
EP2857523A1 (en) 2005-02-01 2015-04-08 Applied Biosystems, LLC Method for identifying a sequence in a polynucleotide
US7824890B2 (en) 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
US9040237B2 (en) 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US8940143B2 (en) 2007-06-29 2015-01-27 Intel Corporation Gel-based bio chip for electrochemical synthesis and electrical detection of polymers
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
AU2006287548A1 (en) 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US8179296B2 (en) 2005-09-30 2012-05-15 The Massachusetts Institute Of Technology Digital readout method and apparatus
US7615382B2 (en) 2005-11-09 2009-11-10 The Board Of Trustees Of The Leland Stanford Junior University Magnetic sifter
EP2578702A1 (en) 2006-02-16 2013-04-10 454 Life Sciences Corporation System and method for correcting primer extension errors in nucleic acid sequence data
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
CN101405083A (zh) 2006-03-21 2009-04-08 皇家飞利浦电子股份有限公司 具有场电极的微电子装置
EP1998892A1 (en) 2006-03-21 2008-12-10 Koninklijke Philips Electronics N.V. Microelectronic device with field electrodes
WO2009052348A2 (en) 2007-10-17 2009-04-23 Advanced Liquid Logic, Inc. Manipulation of beads in droplets
EP2029782B1 (en) 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
US8471230B2 (en) 2006-09-01 2013-06-25 Pacific Biosciences Of California, Inc. Waveguide substrates and optical systems and methods of use thereof
WO2008066747A2 (en) 2006-11-22 2008-06-05 The Board Of Trustees Of Michigan State University Electroluminescent-based fluorescence detection device
US7989396B2 (en) 2006-12-05 2011-08-02 The Board Of Trustees Of The Leland Stanford Junior University Biomolecule immobilization on biosensors
US9188594B2 (en) 2006-12-06 2015-11-17 Yale University Nanoelectronic-enzyme linked immunosorbent assay system and method
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
CA2674219A1 (en) * 2007-01-24 2008-12-18 Arrayomics, Inc. Microdevice arrays formed by magnetic assembly
US7835871B2 (en) 2007-01-26 2010-11-16 Illumina, Inc. Nucleic acid sequencing system and method
US8315817B2 (en) 2007-01-26 2012-11-20 Illumina, Inc. Independently removable nucleic acid sequencing system and method
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
US7731414B2 (en) * 2007-02-08 2010-06-08 Instrumentation Laboratory Company Reagent cartridge mixing tube
WO2008103135A2 (en) 2007-02-16 2008-08-28 The Johns Hopkins University The micrornaome
US9063117B2 (en) 2007-02-21 2015-06-23 Paul L. Gourley Micro-optical cavity with fluidic transport chip for bioparticle analysis
JP4945279B2 (ja) 2007-03-23 2012-06-06 株式会社日立製作所 Dna分析方法および分析装置
US9034637B2 (en) 2007-04-25 2015-05-19 Nxp, B.V. Apparatus and method for molecule detection using nanopores
WO2008147530A1 (en) 2007-05-24 2008-12-04 The Regents Of The University Of California Integrated fluidics devices with magnetic sorting
JP2009002808A (ja) * 2007-06-21 2009-01-08 Hitachi Ltd Dna計測システム及びdna計測方法
JP4876031B2 (ja) 2007-06-22 2012-02-15 株式会社日立製作所 分析装置
WO2009017678A2 (en) 2007-07-26 2009-02-05 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
US20110008775A1 (en) 2007-12-10 2011-01-13 Xiaolian Gao Sequencing of nucleic acids
WO2009074926A1 (en) 2007-12-13 2009-06-18 Nxp B.V. A biosensor device and a method of sequencing biological particles
US8372585B2 (en) * 2007-12-31 2013-02-12 Intel Corporation Electronic sensing for nucleic acid sequencing
US8067176B2 (en) 2008-01-25 2011-11-29 Shimadzu Corporation Microchemistry reaction method
US8173080B2 (en) 2008-02-14 2012-05-08 Illumina, Inc. Flow cells and manifolds having an electroosmotic pump
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
EP2107040B1 (en) 2008-03-31 2011-10-26 Sony Deutschland Gmbh A method of fabricating a membrane having a tapered pore
GB0805814D0 (en) 2008-03-31 2008-04-30 Cambridge Entpr Ltd Biosensor for analyte detection
US8940142B2 (en) 2008-05-05 2015-01-27 The Regents Of The University Of California Functionalized nanopipette biosensor
US8039817B2 (en) 2008-05-05 2011-10-18 Illumina, Inc. Compensator for multiple surface imaging
CA2724408A1 (en) 2008-05-19 2009-11-26 The University Of North Carolina At Chapel Hill Methods and compositions comprising novel cationic lipids
CN104862385B (zh) 2008-06-11 2018-11-23 基因排列技术有限公司 等温核酸扩增
WO2010008480A2 (en) 2008-06-25 2010-01-21 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
EP2294225B8 (en) 2008-06-30 2015-02-18 Life Technologies Corporation Method for direct amplification from crude nucleic acid samples
CA2730068A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20100035252A1 (en) 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
EP2321429B1 (en) 2008-09-03 2016-08-17 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US8945912B2 (en) 2008-09-29 2015-02-03 The Board Of Trustees Of The University Of Illinois DNA sequencing and amplification systems using nanoscale field effect sensor arrays
WO2010039553A1 (en) 2008-10-03 2010-04-08 Illumina, Inc. Method and system for determining the accuracy of dna base identifications
EP2350652A2 (en) * 2008-10-10 2011-08-03 Cnrs-Dae Cell sorting device
US8546128B2 (en) 2008-10-22 2013-10-01 Life Technologies Corporation Fluidics system for sequential delivery of reagents
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010047804A1 (en) 2008-10-22 2010-04-29 Ion Torrent Systems Incorporated Integrated sensor arrays for biological and chemical analysis
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP2607496B1 (en) 2008-12-23 2014-07-16 Illumina, Inc. Methods useful in nucleic acid sequencing protocols
US8563240B2 (en) * 2008-12-31 2013-10-22 Intel Corporation Nucleic acid sequencing and electronic detection
CN103952482A (zh) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 用于对目标核酸进行条形码化的多引物扩增方法
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
EP2435461B1 (en) 2009-05-29 2017-08-09 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2010141391A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
WO2011038197A1 (en) 2009-09-25 2011-03-31 Alere San Diego, Inc. Detection of nucleic acids in crude matrices
US8962279B2 (en) 2009-12-30 2015-02-24 Intel Corporation Solid-phase chelators and electronic biosensors
EP2933629B1 (en) 2010-02-19 2019-04-10 Pacific Biosciences Of California, Inc. System for measuring analytical reactions comprising a socket for an optode array chip
WO2011106556A2 (en) 2010-02-24 2011-09-01 Life Technologies Corporation Systems and methods for adaptive recursive sequencing
US8524064B2 (en) 2010-04-22 2013-09-03 Lawrence Livermore National Security, Llc Three dimensional microelectrode system for dielectrophoresis
US20110287432A1 (en) 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
WO2012024658A2 (en) * 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
US9309569B2 (en) 2010-08-26 2016-04-12 Intelligent Bio-Systems, Inc. Methods and compositions for sequencing nucleic acid using charge
US8444835B2 (en) 2010-09-09 2013-05-21 Intel Corporation Electronic and fluidic interface
AU2011305445B2 (en) 2010-09-24 2017-03-16 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
EP2625526B1 (en) 2010-10-04 2017-03-15 Genapsys Inc. Systems and methods for automated reusable parallel biological reactions
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US20130090860A1 (en) 2010-12-30 2013-04-11 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
WO2012092515A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
GB2488600B (en) 2011-03-04 2013-05-29 Hm Technology Internat Ltd A force sensor
EP3978620A1 (en) 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
US20120264617A1 (en) 2011-04-14 2012-10-18 Pettit John W Dna sequencing employing nanomaterials
US20130059762A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
US9957558B2 (en) 2011-04-28 2018-05-01 Life Technologies Corporation Methods and compositions for multiplex PCR
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
KR101940833B1 (ko) 2011-05-27 2019-01-21 제납시스 인크. 유전자 및 생물학적 분석을 위한 시스템 및 방법
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
WO2013056241A2 (en) 2011-10-14 2013-04-18 Pacific Biosciences Of California, Inc. Real-time redox sequencing
CA2856163C (en) 2011-10-28 2019-05-07 Illumina, Inc. Microarray fabrication system and method
US9689835B2 (en) 2011-10-31 2017-06-27 Taiwan Semiconductor Manufacturing Company, Ltd. Amplified dual-gate bio field effect transistor
CN104105797B (zh) 2011-12-01 2016-08-31 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
US8795605B2 (en) 2012-01-12 2014-08-05 Fred C. Senftleber Apparatus and methods for transferring materials between locations possessing different cross-sectional areas with minimal band spreading and dispersion due to unequal path-lengths
EP2812708B1 (en) 2012-02-10 2019-09-04 The University of North Carolina At Chapel Hill Method of analysing an analyte with fluidic nanofunnels
US8906320B1 (en) 2012-04-16 2014-12-09 Illumina, Inc. Biosensors for biological or chemical analysis and systems and methods for same
JP2015525077A (ja) 2012-06-15 2015-09-03 ジェニア・テクノロジーズ・インコーポレイテッド チップの構成および高精度な核酸配列決定
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
CN110777195A (zh) 2012-07-13 2020-02-11 生命技术公司 采用一组snp的人身份识别
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
ES2750635T3 (es) 2012-10-16 2020-03-26 Abbott Lab Método de aumento de la longitud de Debye sobre la superficie de un sensor dentro de una solución de muestra y el correspondiente medio de almacenamiento legible para una computadora
CN102980922A (zh) 2012-11-21 2013-03-20 济南大学 可寻址电化学传感器阵列的制备及用于多种肿瘤标志物的检测和癌症筛查
US10106842B2 (en) 2013-03-15 2018-10-23 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
EP2971141B1 (en) 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
US20150316502A1 (en) 2013-12-03 2015-11-05 FemtoDx Debye length modulation
EP3080300B1 (en) 2013-12-11 2020-09-02 Genapsys Inc. Systems and methods for biological analysis and computation
EP3117221B1 (en) 2014-03-13 2020-09-09 Genapsys Inc. Microfluidic devices and methods for sample preparation and analysis
EP3132060B1 (en) 2014-04-18 2019-03-13 Genapsys Inc. Methods and systems for nucleic acid amplification
EP3488017A4 (en) 2016-07-20 2020-02-26 Genapsys Inc. SYSTEMS AND METHODS FOR SEQUENCING NUCLEIC ACIDS
EP3684951A4 (en) 2017-09-21 2021-06-16 Genapsys, Inc. NUCLEIC ACID SEQUENCING SYSTEMS AND METHODS

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001018246A1 (en) * 1999-08-26 2001-03-15 The Trustees Of Princeton University Microfluidic and nanofluidic electronic devices for detecting changes in capacitance of fluids and methods of using
WO2001042508A2 (en) * 1999-12-09 2001-06-14 Motorola, Inc. Methods and compositions relating to electrical detection of nucleic acid reactions
US20030209432A1 (en) * 2000-12-11 2003-11-13 Choong Vi-En Methods and compositions relating to electrical detection of nucleic acid reactions
US20050019784A1 (en) * 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
WO2005008450A2 (en) * 2003-03-28 2005-01-27 The Regents Of The University Of California Method and apparatus for nanogap device and array
US20060008824A1 (en) * 2004-05-20 2006-01-12 Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
WO2005121363A2 (en) * 2004-06-11 2005-12-22 Medical Biosystems Limited Method for determining biophysical properties
US20070184463A1 (en) * 2005-09-30 2007-08-09 Caliper Life Sciences, Inc. Microfluidic device for purifying a biological component using magnetic beads
WO2008108612A1 (es) * 2007-03-06 2008-09-12 Salinas Garcia Jose Marco Pieza para la fijación y el aislamiento de conductores eléctricos
US20080241841A1 (en) * 2007-03-30 2008-10-02 Hitachi, Ltd. Method and apparatus for sample preparation
WO2009012112A1 (en) * 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
US20100078325A1 (en) * 2008-09-03 2010-04-01 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US20100112588A1 (en) * 2008-11-04 2010-05-06 Caerus Molecular Diagnostics, Inc. Methods for sanger sequencing using particle associated clonal amplicons and highly parallel electrophoretic size-based separation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Nucl. Acids Res., Vol.25, 1997, Edman, C. F. et al., "Electric field directed...", pp.4907-4914 *
P.N.A.S., Vol.94, 1997, Sosnowski, R. G. et al., "Rapid determination of single base...", pp.1119-1123 *

Also Published As

Publication number Publication date
US20150368707A1 (en) 2015-12-24
MX2013003860A (es) 2013-11-04
US9187783B2 (en) 2015-11-17
JP6114694B2 (ja) 2017-04-12
AU2011312218A1 (en) 2013-05-30
EP2625526A2 (en) 2013-08-14
NZ610129A (en) 2014-08-29
US10472674B2 (en) 2019-11-12
WO2012047889A3 (en) 2012-07-12
JP2013539978A (ja) 2013-10-31
CN103328981B (zh) 2017-04-12
GB2499340A (en) 2013-08-14
GB201308138D0 (en) 2013-06-12
US9150915B2 (en) 2015-10-06
CN103328981A (zh) 2013-09-25
AU2011312218B2 (en) 2015-11-05
US20170073750A1 (en) 2017-03-16
WO2012047889A2 (en) 2012-04-12
KR101963462B1 (ko) 2019-03-28
US20190226021A1 (en) 2019-07-25
US20160076097A1 (en) 2016-03-17
US20150148264A1 (en) 2015-05-28
US10100356B2 (en) 2018-10-16
KR20140027906A (ko) 2014-03-07
US9533305B2 (en) 2017-01-03
EP2625526B1 (en) 2017-03-15
MX344363B (es) 2016-12-14
IL225558B (en) 2018-12-31
US8969002B2 (en) 2015-03-03
SG189839A1 (en) 2013-06-28
US20140045701A1 (en) 2014-02-13
IL225558A0 (en) 2013-06-27
HK1188614A1 (en) 2014-05-09
US20130096013A1 (en) 2013-04-18
EP2625526A4 (en) 2014-04-09

Similar Documents

Publication Publication Date Title
IL280133A (en) Methods and devices for the synthesis of nucleic acids
EP2694679A4 (en) METHODS AND SYSTEMS FOR LONG NUCLEIC ACID SEQUENCING
PT3623481T (pt) Composições para sequenciação de ácidos nucleicos
GB2498163B (en) High-throughput immune sequencing
EP2890966A4 (en) PROCESS AND KIT FOR CLEANING NUCLEIC ACIDS
PL3018211T3 (pl) Antysensowne nici kwasu nukleinowego
IL231983B (en) Dna containing tricyclo-phosphorothioate
ZA201205891B (en) Dna polymerases
EP2831283A4 (en) METHODS AND COMPOSITION FOR SEQUENCING MODIFIED NUCLEIC ACIDS
GB2537077B (en) Methods for sequencing nucleic acids
IL223257A0 (en) Method of dna sequencing by hybridisation
GB201119390D0 (en) Novel methods for determining nucleotide sequence repeats
IL223256A0 (en) Method of dna sequencing by polymerisation
GB201020095D0 (en) Nucleic acid preparation method
EP2740804A4 (en) PROCESS FOR NUCLEIC ACID ANALYSIS
GB2502195B (en) Purification of nucleic acids
EP2844775A4 (en) DNA SEQUENCING
GB2503856B (en) Methods for nucleic acid purification
GB0905458D0 (en) Method of altering nucleic acids
EP2594592A4 (en) COMPLEX (NUCLEIC ACID) -POLYSACCHARIDE
PL2556168T3 (pl) Sposób detekcji specyficznych sekwencji kwasu nukleinowego
GB0909660D0 (en) Method of altering nucleic acids
HK1188614B (en) Methods for sequencing nucleic acids
GB201016452D0 (en) Rapid extrection of nucleic acids
GB201107644D0 (en) Methods and compositions for identifying nucleic acids

Legal Events

Date Code Title Description
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1188614

Country of ref document: HK

R108 Alteration of time limits (patents rules 1995)

Free format text: EXTENSION ALLOWED

Effective date: 20150609

Free format text: EXTENSION APPLICATION

Effective date: 20150528

R108 Alteration of time limits (patents rules 1995)

Free format text: EXTENSION APPLICATION

Effective date: 20150715

Free format text: EXTENSION ALLOWED

Effective date: 20150727

REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1188614

Country of ref document: HK

732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)

Free format text: REGISTERED BETWEEN 20230216 AND 20230222

PCNP Patent ceased through non-payment of renewal fee

Effective date: 20231004

S73 Revocation on comptroller's initiative (section 73/patents act 1977)

Free format text: REVOCATION NOT PROCEEDED WITH