FI20096288A0 - Formulations and methods for culturing stem cells - Google Patents

Formulations and methods for culturing stem cells

Info

Publication number
FI20096288A0
FI20096288A0 FI20096288A FI20096288A FI20096288A0 FI 20096288 A0 FI20096288 A0 FI 20096288A0 FI 20096288 A FI20096288 A FI 20096288A FI 20096288 A FI20096288 A FI 20096288A FI 20096288 A0 FI20096288 A0 FI 20096288A0
Authority
FI
Finland
Prior art keywords
formulations
methods
stem cells
culturing stem
culturing
Prior art date
Application number
FI20096288A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Inventor
Kristiina Rajala
Marjo-Riitta Suuronen
Outi Hovatta
Heli Skottman
Original Assignee
Kristiina Rajala
Marjo Riitta Suuronen
Outi Hovatta
Heli Skottman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kristiina Rajala, Marjo Riitta Suuronen, Outi Hovatta, Heli Skottman filed Critical Kristiina Rajala
Priority to FI20096288A priority Critical patent/FI20096288A0/fi
Publication of FI20096288A0 publication Critical patent/FI20096288A0/fi
Priority to CN2010800545573A priority patent/CN102906247A/zh
Priority to AU2010326512A priority patent/AU2010326512A1/en
Priority to EP10800970A priority patent/EP2507360A1/fr
Priority to PCT/FI2010/050980 priority patent/WO2011067465A1/fr
Priority to KR1020127017428A priority patent/KR20130009943A/ko
Priority to JP2012541546A priority patent/JP2013512667A/ja
Priority to CA2782296A priority patent/CA2782296A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FI20096288A 2009-12-04 2009-12-04 Formulations and methods for culturing stem cells FI20096288A0 (fi)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FI20096288A FI20096288A0 (fi) 2009-12-04 2009-12-04 Formulations and methods for culturing stem cells
CN2010800545573A CN102906247A (zh) 2009-12-04 2010-11-30 用于培养干细胞的制剂和方法
AU2010326512A AU2010326512A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
EP10800970A EP2507360A1 (fr) 2009-12-04 2010-11-30 Formulations et procedes de culture de cellules souches
PCT/FI2010/050980 WO2011067465A1 (fr) 2009-12-04 2010-11-30 Formulations et procedes de culture de cellules souches
KR1020127017428A KR20130009943A (ko) 2009-12-04 2010-11-30 줄기세포 배양을 위한 제형 및 방법
JP2012541546A JP2013512667A (ja) 2009-12-04 2010-11-30 幹細胞を培養するための処方物および方法
CA2782296A CA2782296A1 (fr) 2009-12-04 2010-11-30 Formulations et procedes de culture de cellules souches

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI20096288A FI20096288A0 (fi) 2009-12-04 2009-12-04 Formulations and methods for culturing stem cells

Publications (1)

Publication Number Publication Date
FI20096288A0 true FI20096288A0 (fi) 2009-12-04

Family

ID=41462754

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20096288A FI20096288A0 (fi) 2009-12-04 2009-12-04 Formulations and methods for culturing stem cells

Country Status (8)

Country Link
EP (1) EP2507360A1 (fr)
JP (1) JP2013512667A (fr)
KR (1) KR20130009943A (fr)
CN (1) CN102906247A (fr)
AU (1) AU2010326512A1 (fr)
CA (1) CA2782296A1 (fr)
FI (1) FI20096288A0 (fr)
WO (1) WO2011067465A1 (fr)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
CN101952415B (zh) 2007-07-31 2017-06-27 生命扫描有限公司 人胚胎干细胞的分化
CN107574142B (zh) 2007-11-27 2021-07-06 生命扫描有限公司 人胚胎干细胞的分化
WO2009105570A2 (fr) 2008-02-21 2009-08-27 Centocor Ortho Biotech Inc. Procédés, plaques à surface modifiée et compositions permettant la fixation, la culture et le détachement de cellules
JP5734183B2 (ja) 2008-06-30 2015-06-17 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞の分化
CA2742267C (fr) 2008-10-31 2019-06-04 Centocor Ortho Biotech Inc. Differenciation de cellules souches embryonnaires humaines en la lignee endocrine pancreatique
BRPI0919885A2 (pt) 2008-10-31 2015-08-11 Centocor Ortho Biotech Inc Diferenciação de células-tronco embrionárias humanas para a linhagem endócrina pancreática
AU2009316583B2 (en) 2008-11-20 2016-04-21 Janssen Biotech, Inc. Methods and compositions for cell attachment and cultivation on planar substrates
RU2555538C2 (ru) 2008-11-20 2015-07-10 Сентокор Орто Байотек Инк. Культура плюрипотентных стволовых клеток на микроносителях
EP2456862A4 (fr) 2009-07-20 2013-02-27 Janssen Biotech Inc Différentiation de cellules souches embryonnaires humaines
CN102741395B (zh) 2009-12-23 2016-03-16 詹森生物科技公司 人胚胎干细胞的分化
AU2011223900A1 (en) 2010-03-01 2012-09-13 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
MX351515B (es) 2010-05-12 2017-10-17 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas.
AU2011296381B2 (en) 2010-08-31 2016-03-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
US9528090B2 (en) 2010-08-31 2016-12-27 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP6148429B2 (ja) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 ヒト多能性幹細胞の培養方法
AU2012355698B2 (en) 2011-12-22 2018-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
KR20140131999A (ko) 2012-03-07 2014-11-14 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 증폭 및 유지를 위한 한정 배지
CN108103006A (zh) 2012-06-08 2018-06-01 詹森生物科技公司 人胚胎干细胞向胰腺内分泌细胞的分化
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
KR102084561B1 (ko) 2012-12-31 2020-03-04 얀센 바이오테크 인코포레이티드 췌장 내분비 세포로의 분화를 위한 공기-액체 계면에서의 인간 배아 줄기세포의 배양
KR102036780B1 (ko) 2012-12-31 2019-10-25 얀센 바이오테크 인코포레이티드 Hb9 조절제를 사용하는 인간 배아 줄기세포의 췌장 내분비 세포로의 분화
JP6529440B2 (ja) 2012-12-31 2019-06-12 ヤンセン バイオテツク，インコーポレーテツド 膵内分泌細胞への分化のためのヒト多能性細胞の懸濁及びクラスタリング
SG11201509767UA (en) 2013-05-30 2015-12-30 Ajinomoto Kk Medium for culturing stem cells
CN103432108A (zh) * 2013-07-16 2013-12-11 江苏大学 N-3不饱和脂肪酸在制备促造血干细胞动员剂中的用途
MX2016008251A (es) * 2013-12-20 2016-10-13 Essential Pharmaceuticals Llc Medios para cultivo celular.
CA2949056A1 (fr) 2014-05-16 2015-11-19 Janssen Biotech, Inc. Utilisation de petites molecules pour ameliorer l'expression du gene mafa dans des cellules endocrines pancreatiques
WO2016088243A1 (fr) * 2014-12-05 2016-06-09 株式会社ニコン Dispositif de détermination, système d'observation, procédé d'observation, programme associé, procédé de fabrication de cellule, et cellule
WO2017146468A1 (fr) * 2016-02-23 2017-08-31 경희대학교 산학협력단 Composition et procédé pour améliorer l'efficacité de cellules souches
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
KR101896803B1 (ko) * 2016-12-12 2018-09-07 서울대학교병원 인간 만능줄기세포로부터 중간엽 줄기세포로의 분화 유도 생산율을 증가시키는 방법 및 이에 의해 생성된 중간엽 줄기세포
JP7049347B2 (ja) * 2017-01-23 2022-04-06 ステムセル テクノロジーズ カナダ インコーポレイテッド 幹細胞の生存および増殖を高めるための培地および方法
KR102623647B1 (ko) * 2017-06-27 2024-01-12 아지노모토 가부시키가이샤 리보플라빈 유도체 함유 배지
AU2018348712A1 (en) * 2017-10-13 2020-03-26 Boehringer Ingelheim International Gmbh Perfusion medium
JP2020162536A (ja) * 2019-03-29 2020-10-08 興人ライフサイエンス株式会社 組み換えタンパク質産生増加のための培地用組成物
JP2023505988A (ja) * 2019-12-09 2023-02-14 デウン ファーマシューティカル カンパニー リミテッド ヒト多能性幹細胞から間葉系幹細胞の製造方法およびこれによって製造された間葉系幹細胞
JPWO2023286772A1 (fr) * 2021-07-12 2023-01-19
CN113832097B (zh) * 2021-09-07 2024-02-20 生物岛实验室 组合物及含有其的无血清、无饲养层干细胞培养基及应用
CN115025122A (zh) * 2022-06-25 2022-09-09 广东旺合生物科技有限公司 自体脂肪干细胞在制备抗衰老和抗免疫低下的药物或制品中的应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
WO2003018780A1 (fr) 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. Dedifferenciation et redifferenciation de cellules somatiques et production de cellules pour des therapies cellulaires
MX2007002390A (es) 2004-09-08 2007-04-23 Wisconsin Alumni Res Found Medio y cultivo de celulas progenitoras embrionarias.
DE102005054577A1 (de) * 2005-11-16 2007-05-24 Cognis Ip Management Gmbh Verwendung von Estern ungesättigter, physiologisch aktiver Fettsäuren als Nährmedien für Zellkulturen
TW200801513A (en) 2006-06-29 2008-01-01 Fermiscan Australia Pty Ltd Improved process
GB0622394D0 (en) * 2006-11-09 2006-12-20 Univ Cambridge Tech Differentiation of pluripotent cells
FI20075417A0 (fi) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen

Also Published As

Publication number Publication date
JP2013512667A (ja) 2013-04-18
CA2782296A1 (fr) 2011-06-09
CN102906247A (zh) 2013-01-30
WO2011067465A1 (fr) 2011-06-09
KR20130009943A (ko) 2013-01-24
EP2507360A1 (fr) 2012-10-10
AU2010326512A1 (en) 2012-06-28

Similar Documents

Publication Publication Date Title
FI20096288A0 (fi) Formulations and methods for culturing stem cells
BRPI1011127A2 (pt) métodos para o cultivo de células tronco e células progenitoras
IL214798A0 (en) Methods and compositions for stem cell cultures
HK1216883A1 (zh) 幹細胞培養
GB2458863B (en) Improved culture of stem cells
EP2155859A4 (fr) Formulations et procédés pour cultiver des cellules souches embryonnaires
DK3192865T3 (da) Enkelt pluripotent stamcelle-kultur
EP2487236A4 (fr) Substrat de culture pour cellules souches pluripotentes humaines, et son utilisation
SG10201500674YA (en) Pluripotent stem cells
EP2315829A4 (fr) Cellules souches pluripotentes induites
BRPI1013829A2 (pt) composições e métodos para fermentação de biomassa
DK2574677T3 (da) Fremgangsmåder til celledyrkning
EP2314671A4 (fr) Procédé de culture de cellule souche
HK1246356A1 (zh) 微載體上的多能幹細胞培養
DK2614141T3 (da) Hidtil ukendte fremgangsmåder og dyrkningsmedier til dyrkning af pluripotente stamceller
DK2356213T3 (da) Stamcelleaggregatsuspensionssammensætninger og fremgangsmåder til differentiering deraf
BR112013001089A2 (pt) composição de meio de cultura e método para cultivar células tronco mesenquimais derivadas de âmnio
BRPI0913925A2 (pt) Diferenciação de células-tronco pluripotentes
SG10201405455UA (en) Cells and methods for obtaining them
EP2424978A4 (fr) Compositions et méthodes permettant de moduler les cellules souches et leurs utilisations
EP2428562A4 (fr) Méthode de culture de cellules souches
GB0810260D0 (en) Automated culture of stem cells
EP2569420A4 (fr) Procédé et composition utilisés en vue de l'induction de cellules souches pluripotentes humaines
EP2294182A4 (fr) Cultures de cellules souches multipotentes
EP2476748A4 (fr) Méthode de culture de cellules souches hématopoïétiques

Legal Events

Date Code Title Description
FD Application lapsed