DK2614141T3 - Hidtil ukendte fremgangsmåder og dyrkningsmedier til dyrkning af pluripotente stamceller - Google Patents

Hidtil ukendte fremgangsmåder og dyrkningsmedier til dyrkning af pluripotente stamceller Download PDF

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DK2614141T3
DK2614141T3 DK11770876.8T DK11770876T DK2614141T3 DK 2614141 T3 DK2614141 T3 DK 2614141T3 DK 11770876 T DK11770876 T DK 11770876T DK 2614141 T3 DK2614141 T3 DK 2614141T3
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stem cells
pluripotent stem
cultivation media
culturing pluripotent
present procedures
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DK11770876.8T
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Michal Amit
Joseph Itskovitz-Eldor
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Technion Res & Dev Foundation
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DK11770876.8T 2010-09-07 2011-09-07 Hidtil ukendte fremgangsmåder og dyrkningsmedier til dyrkning af pluripotente stamceller DK2614141T3 (da)

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US38038810P 2010-09-07 2010-09-07
PCT/IL2011/000722 WO2012032521A2 (en) 2010-09-07 2011-09-07 Novel methods and culture media for culturing pluripotent stem cells

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DK2614141T3 true DK2614141T3 (da) 2019-09-09

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US (5) US10214722B2 (da)
EP (2) EP2614141B1 (da)
JP (5) JP6283223B2 (da)
CA (2) CA2810488A1 (da)
DK (1) DK2614141T3 (da)
ES (1) ES2746482T3 (da)
IL (4) IL225070B (da)
SG (4) SG10201800628QA (da)
WO (1) WO2012032521A2 (da)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007120811A2 (en) 2006-04-14 2007-10-25 Advanced Cell Technology, Inc. Hemangio-colony forming cells
ES2779048T3 (es) 2009-11-12 2020-08-13 Technion Res & Dev Foundation Medios de cultivo, cultivos celulares y métodos de cultivo de células madre pluripotentes en un estado indiferenciado
SG10201800628QA (en) 2010-09-07 2018-02-27 Technion Res & Dev Foundation Novel Methods And Culture Media For Culturing Pluripotent Stem Cells
JP5745919B2 (ja) * 2011-04-28 2015-07-08 浜松ホトニクス株式会社 細胞解析方法、細胞解析装置、および細胞解析プログラム
US9175263B2 (en) * 2012-08-22 2015-11-03 Biotime, Inc. Methods and compositions for targeting progenitor cell lines
US10961531B2 (en) 2013-06-05 2021-03-30 Agex Therapeutics, Inc. Compositions and methods for induced tissue regeneration in mammalian species
US11078462B2 (en) 2014-02-18 2021-08-03 ReCyte Therapeutics, Inc. Perivascular stromal cells from primate pluripotent stem cells
WO2015181253A1 (en) * 2014-05-27 2015-12-03 Fundación Pública Andaluza Progreso Y Salud Neural progenitor cell population
US10240127B2 (en) 2014-07-03 2019-03-26 ReCyte Therapeutics, Inc. Exosomes from clonal progenitor cells
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
WO2016159179A1 (ja) * 2015-03-30 2016-10-06 味の素株式会社 ヒト血清アルブミンを含む神経幹細胞増殖培地
WO2016183593A2 (en) * 2015-05-14 2016-11-17 The Regents Of The University Of California Prenatal therapy
CN105002134B (zh) * 2015-07-24 2018-08-17 北京大学 制备多潜能干细胞的组合物、方法、试剂盒及其用途
CA2996582A1 (en) * 2015-08-31 2017-03-09 I Peace, Inc. Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
US20200008405A1 (en) * 2016-01-13 2020-01-09 Felix Pets, Llc Methods for producing hypo-allergenic cats using gene editing technology
KR101855967B1 (ko) 2016-03-11 2018-05-10 가톨릭관동대학교산학협력단 물리적 자극에 의한 환경유입을 이용한 세포 리프로그래밍 방법
WO2017172638A1 (en) * 2016-03-29 2017-10-05 Smsbiotech, Inc. Compositions and methods for using small mobile stem cells
CA3030252A1 (en) * 2016-07-19 2018-01-25 Accellta Ltd. Culture media for culturing pluripotent stem cells in suspension
JPWO2018194161A1 (ja) * 2017-04-20 2020-02-27 国立大学法人東北大学 振盪浮遊培養を用いた間葉系幹細胞の未分化性維持方法
JPWO2019181999A1 (ja) * 2018-03-20 2021-02-04 富士フイルム株式会社 多能性幹細胞を三次元培養する方法
KR102175792B1 (ko) * 2018-11-20 2020-11-06 사회복지법인 삼성생명공익재단 줄기세포의 증식능을 탐지하기 위한 마커 및 이를 이용한 줄기세포의 고효율 증식방법
US20230091910A1 (en) * 2019-04-02 2023-03-23 Centagen, Inc. Engineered System of Stem Cell Rejuvenation to Treat Aging and Disease
US11826584B2 (en) 2019-04-04 2023-11-28 Synaptec Network, Inc. Systems and methods for using low intensity ultrasonic transducer on the brain
CN114401740A (zh) * 2019-05-30 2022-04-26 伊美诺路克斯国际公司 痘病毒与自体诱导性多能干细胞用于疫苗接种和疾病治疗的用途
EP4092101A4 (en) * 2020-01-14 2024-03-06 Ajinomoto Co., Inc. CELL CULTURE METHOD
KR20220128647A (ko) * 2020-01-14 2022-09-21 아지노모토 가부시키가이샤 세포 배양용 배지 조성물
US20230094568A1 (en) * 2020-02-12 2023-03-30 Kaneka Corporation Method for suppressing differentiation of pluripotent stem cells
IL299625A (en) * 2020-07-02 2023-03-01 Accellta Ltd Mammalian pluripotent stem cells from late embryos
US20220033771A1 (en) * 2020-08-03 2022-02-03 So Young Life Sciences Corporation Combination Treatment Of Induced Pluripotent Stem Cells Using Interleukins
JP2024520792A (ja) * 2021-06-09 2024-05-24 アクセルタ リミテッド 哺乳類家畜多能性幹細胞の懸濁培養のための無血清培地
CN114350599B (zh) * 2022-01-14 2024-01-19 内蒙古大学 一种增强牛胚胎干细胞的多能性和代谢活力的培养体系和培养方法
WO2023229819A1 (en) * 2022-05-24 2023-11-30 Bluerock Therapeutics Lp Methods of making oligodendrocyte progenitor cells
WO2024102954A1 (en) 2022-11-10 2024-05-16 Massachusetts Institute Of Technology Activation induced clipping system (aics)

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
IL68218A (en) 1983-03-23 1985-12-31 Univ Ramot Compositions for cartilage repair comprising embryonal chondrocytes
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US4866042A (en) 1987-11-18 1989-09-12 Neuwelt Edward A Method for the delivery of genetic material across the blood brain barrier
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5489304A (en) 1994-04-19 1996-02-06 Brigham & Women's Hospital Method of skin regeneration using a collagen-glycosaminoglycan matrix and cultured epithelial autograft
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
IL122818A0 (en) 1997-07-10 1998-08-16 Yeda Res & Dev Chimeric interleukin-6 soluble receptor/ligand protein analogs thereof and uses thereof
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US7455983B2 (en) * 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
WO2003006950A2 (en) 2001-07-12 2003-01-23 Geron Corporation Cells of the cardiomyocyte lineage produced from human pluripotent stem cells
KR20040023724A (ko) 2001-08-07 2004-03-18 기린 비루 가부시키가이샤 조혈 간세포의 제조법
WO2003046141A2 (en) * 2001-11-26 2003-06-05 Advanced Cell Technology, Inc. Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
WO2004085631A2 (en) * 2003-03-28 2004-10-07 Mount Sinai Hospital Canine embryonic stem cells
KR101121971B1 (ko) 2003-07-22 2012-03-09 비바리스 부착성 또는 비부착성 조류 세포주를 이용한폭스바이러스의 생산
WO2005065354A2 (en) 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
CN1934245B (zh) 2004-03-23 2012-07-04 第一三共株式会社 多能干细胞的增殖方法
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
EP1799810A2 (en) 2004-10-12 2007-06-27 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
WO2006079854A1 (en) 2005-01-28 2006-08-03 Novathera Ltd Methods for embryonic stem cell culture
WO2006084314A1 (en) 2005-02-09 2006-08-17 Australian Stem Cell Centre Limited Stem cell populations and classification system
GB0505970D0 (en) 2005-03-23 2005-04-27 Univ Edinburgh Culture medium containing kinase inhibitor, and uses thereof
EP3354723B1 (en) 2005-08-29 2023-12-13 Technion Research & Development Foundation Ltd. Media for culturing stem cells
EP2733203B1 (en) 2006-08-02 2018-10-10 Technion Research & Development Foundation Ltd. Methods of expanding embryonic stem cells in a suspension culture
JP5991796B2 (ja) 2007-06-29 2016-09-14 セルラー ダイナミクス インターナショナル， インコーポレイテッド 胚性幹細胞培養のための自動化された方法および装置
WO2010108126A2 (en) 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Reprogramming compositions and methods of using the same
US8524498B2 (en) * 2009-05-29 2013-09-03 The General Hospital Corporation Methods and compositions for homologous recombination in human cells
ES2779048T3 (es) 2009-11-12 2020-08-13 Technion Res & Dev Foundation Medios de cultivo, cultivos celulares y métodos de cultivo de células madre pluripotentes en un estado indiferenciado
SG10201800628QA (en) 2010-09-07 2018-02-27 Technion Res & Dev Foundation Novel Methods And Culture Media For Culturing Pluripotent Stem Cells

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