FI120741B - DNA:n vahvistaminen - Google Patents
DNA:n vahvistaminen Download PDFInfo
- Publication number
- FI120741B FI120741B FI953079A FI953079A FI120741B FI 120741 B FI120741 B FI 120741B FI 953079 A FI953079 A FI 953079A FI 953079 A FI953079 A FI 953079A FI 120741 B FI120741 B FI 120741B
- Authority
- FI
- Finland
- Prior art keywords
- sequence
- dna
- dna sequence
- cell
- homologous
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Semiconductor Lasers (AREA)
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK921539A DK153992D0 (da) | 1992-12-22 | 1992-12-22 | Metode |
DK153992 | 1992-12-22 | ||
PCT/DK1993/000438 WO1994014968A1 (en) | 1992-12-22 | 1993-12-22 | Dna amplification |
DK9300438 | 1993-12-22 | ||
CN94109005A CN1063488C (zh) | 1992-12-22 | 1994-06-22 | Dna扩增 |
CN94109005 | 1994-06-22 |
Publications (3)
Publication Number | Publication Date |
---|---|
FI953079A0 FI953079A0 (fi) | 1995-06-21 |
FI953079A FI953079A (fi) | 1995-06-21 |
FI120741B true FI120741B (fi) | 2010-02-15 |
Family
ID=37075831
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI953079A FI120741B (fi) | 1992-12-22 | 1995-06-21 | DNA:n vahvistaminen |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0675959B1 (zh) |
JP (1) | JP3983798B2 (zh) |
CN (1) | CN1063488C (zh) |
AT (1) | ATE323167T1 (zh) |
AU (1) | AU679707B2 (zh) |
CA (1) | CA2152371C (zh) |
DE (1) | DE69334006T2 (zh) |
DK (2) | DK153992D0 (zh) |
FI (1) | FI120741B (zh) |
NO (1) | NO952496L (zh) |
WO (1) | WO1994014968A1 (zh) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR960701200A (ko) * | 1993-03-15 | 1996-02-24 | 스티븐 에이. 쉐어윈 | Dna의 예정-결실 방법(method for defined deletions of dna) |
EP0817856A1 (en) * | 1995-03-22 | 1998-01-14 | Novo Nordisk A/S | Introduction of dna into bacillus strains by conjugation |
FR2762842B1 (fr) | 1997-05-05 | 1999-07-23 | Pasteur Institut | Procede de detection de la presence de matieres biologiques d'origine bovine, et oligonucleotides pour sa mise en oeuvre |
CN1261918A (zh) * | 1997-07-03 | 2000-08-02 | 诺沃挪第克公司 | 具有多拷贝目的基因和可筛选标记而不具选择性标记的产蛋白质细胞 |
AU753578B2 (en) | 1997-12-22 | 2002-10-24 | Novozymes A/S | Carbohydrate oxidase and use thereof in baking |
JP2004501651A (ja) | 2000-06-23 | 2004-01-22 | ノボザイムス アクティーゼルスカブ | 遺伝子の安定した染色体多コピー組み込みのための方法 |
AU2003214036A1 (en) | 2002-04-10 | 2003-10-27 | Novozymes A/S | Bacillus licheniformis mutant host cell |
CN1659283A (zh) | 2002-04-10 | 2005-08-24 | 诺维信公司 | 地衣芽孢杆菌的突变宿主细胞 |
WO2005042750A1 (en) | 2003-10-31 | 2005-05-12 | Novozymes A/S | Method for stable gene-amplification in a bacterial host cell |
EP1707626A4 (en) * | 2003-11-25 | 2008-07-23 | Japan Science & Tech Agency | gene amplification |
WO2005123915A1 (en) | 2004-06-21 | 2005-12-29 | Novozymes A/S | Stably maintained multiple copies of at least two orf in the same orientation |
JP4821012B2 (ja) * | 2005-11-24 | 2011-11-24 | 大学共同利用機関法人自然科学研究機構 | 遺伝子増幅法 |
CN103255114B (zh) | 2006-11-29 | 2015-03-25 | 诺维信股份有限公司 | 改进向细菌细胞中导入dna的方法 |
CN101611145B (zh) | 2006-12-21 | 2013-08-14 | 诺维信股份有限公司 | 用于在细菌细胞中表达基因的修饰型信使rna稳定化序列 |
WO2010097436A1 (en) | 2009-02-27 | 2010-09-02 | Novozymes A/S | Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production |
EP2516636A1 (en) | 2009-12-21 | 2012-10-31 | Novozymes Inc. | Methods for producing heterologous polypeptides in thiol-disulfide oxidoreductase-deficient bacterial mutant cells |
US20140141471A1 (en) | 2011-05-19 | 2014-05-22 | Novozymes, Inc. | Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins |
EP3532619A1 (en) | 2016-10-25 | 2019-09-04 | Novozymes A/S | Flp-mediated genomic integration |
CN107287331B (zh) * | 2017-08-01 | 2020-06-26 | 北京大学 | 一种高效检测蓝藻细胞周期的方法 |
CN111511908A (zh) | 2017-11-10 | 2020-08-07 | 诺维信公司 | 温度敏感性cas9蛋白 |
US20210017544A1 (en) | 2017-12-22 | 2021-01-21 | Novozymes A/S | Counter-Selection by Inhibition of Conditionally Essential Genes |
CN113366113A (zh) | 2019-01-30 | 2021-09-07 | 诺维信公司 | 同源折叠酶共表达 |
EP3935160A1 (en) | 2019-03-08 | 2022-01-12 | Novozymes A/S | Means and methods for improving protease expression |
CN113939588A (zh) | 2019-05-15 | 2022-01-14 | 诺维信公司 | 温度敏感性的rna指导的内切核酸酶 |
EP3990629A1 (en) | 2019-06-25 | 2022-05-04 | Novozymes A/S | Counter-selection by inhibition of conditionally essential genes |
EP4370534A1 (en) | 2021-07-13 | 2024-05-22 | Novozymes A/S | Recombinant cutinase expression |
WO2023247514A1 (en) | 2022-06-22 | 2023-12-28 | Novozymes A/S | Recombinant mannanase expression |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2563533B1 (fr) * | 1984-04-27 | 1986-08-22 | Centre Nat Rech Scient | Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues |
AU620326B2 (en) * | 1987-02-27 | 1992-02-20 | Dsm Ip Assets B.V. | Stable gene amplification in chromosomal dna of prokaryotic microorganisms |
DK0779362T4 (da) * | 1989-12-22 | 2012-07-30 | Serono Lab | DNA-konstruktioner til aktivering og modifikation af endogene geners ekspression |
-
1992
- 1992-12-22 DK DK921539A patent/DK153992D0/da not_active Application Discontinuation
-
1993
- 1993-12-22 AT AT94902652T patent/ATE323167T1/de not_active IP Right Cessation
- 1993-12-22 CA CA002152371A patent/CA2152371C/en not_active Expired - Fee Related
- 1993-12-22 WO PCT/DK1993/000438 patent/WO1994014968A1/en active IP Right Grant
- 1993-12-22 JP JP51470694A patent/JP3983798B2/ja not_active Expired - Fee Related
- 1993-12-22 DK DK94902652T patent/DK0675959T3/da active
- 1993-12-22 EP EP94902652A patent/EP0675959B1/en not_active Expired - Lifetime
- 1993-12-22 AU AU56944/94A patent/AU679707B2/en not_active Ceased
- 1993-12-22 DE DE69334006T patent/DE69334006T2/de not_active Expired - Lifetime
-
1994
- 1994-06-22 CN CN94109005A patent/CN1063488C/zh not_active Expired - Fee Related
-
1995
- 1995-06-21 NO NO952496A patent/NO952496L/no not_active Application Discontinuation
- 1995-06-21 FI FI953079A patent/FI120741B/fi not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP0675959B1 (en) | 2006-04-12 |
NO952496D0 (no) | 1995-06-21 |
FI953079A0 (fi) | 1995-06-21 |
NO952496L (no) | 1995-08-21 |
CN1114355A (zh) | 1996-01-03 |
DK0675959T3 (da) | 2006-08-07 |
AU5694494A (en) | 1994-07-19 |
CN1063488C (zh) | 2001-03-21 |
CA2152371C (en) | 2008-12-02 |
JP3983798B2 (ja) | 2007-09-26 |
DE69334006T2 (de) | 2006-11-23 |
ATE323167T1 (de) | 2006-04-15 |
DE69334006D1 (de) | 2006-05-24 |
WO1994014968A1 (en) | 1994-07-07 |
FI953079A (fi) | 1995-06-21 |
DK153992D0 (da) | 1992-12-22 |
CA2152371A1 (en) | 1994-07-07 |
AU679707B2 (en) | 1997-07-10 |
JPH08504585A (ja) | 1996-05-21 |
EP0675959A1 (en) | 1995-10-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
FI120741B (fi) | DNA:n vahvistaminen | |
US5733753A (en) | Amplification of genomic DNA by site specific integration of a selectable marker construct | |
EP3348638B1 (en) | Method for converting genome sequence of gram-positive bacterium by specifically converting nucleic acid base of targeted dna sequence, and molecular complex used in same | |
JP3162707B2 (ja) | 細菌ゲノムにおけるdnaの安定な組込み | |
KR101311619B1 (ko) | 클로스트리디아에서 dna 서열 대체 및 염색체 통합하기 위한 방법 | |
JP3529774B2 (ja) | バシラス・リヘニフォルミスx−アミラーゼのプロモーターの変異体由来のバシラス属細菌のプロモーター | |
JP2004501651A (ja) | 遺伝子の安定した染色体多コピー組み込みのための方法 | |
US5958728A (en) | Methods for producing polypeptides in mutants of bacillus cells | |
JP2001503641A (ja) | バシラス細胞のサーファクチン突然変異株におけるポリペプチドの製造方法 | |
CN101978057B (zh) | 修饰宿主dna中的对象区域的方法和选择性标记盒 | |
US5925544A (en) | Method of homologous recombination followed by in vivo selection of DNA amplification | |
US5695976A (en) | Stable integration of DNA in bacterial genomes | |
Olson et al. | High-level expression of eukaryotic polypeptides from bacterial chromosomes | |
US6100063A (en) | Procaryotic cell comprising at least two copies of a gene | |
JP7312741B2 (ja) | 望ましい遺伝子発現を促進するためのプラスミドアディクションシステム | |
HU197937B (en) | Process for producing new cloning carriers usable for the expression of polypeptides in microbiological host cells | |
Masai et al. | Mechanisms of primer RNA synthesis and D-loop/R-loop-dependent DNA replication in Escherichia coli | |
EP0228726B1 (en) | Method for preparing proteins using transformed lactic acid bacteria | |
JP4350894B2 (ja) | 異なる方向に転写される2コピーの遺伝子を含有する原核細胞 | |
JP3058186B2 (ja) | 新規なクローニング及び/又は発現ベクター、それらの製造方法及びそれらの使用 | |
KR100738002B1 (ko) | 로도코커스―대장균 셔틀벡터 | |
Weng et al. | Transcriptional Analysis of the Threonine Dehydrogenase Gene ofXanthomonas campestris | |
JP5785787B2 (ja) | プロトプラスト形質転換効率の向上方法 | |
JP4589540B2 (ja) | 組換え遺伝子形成方法 | |
Kabisch | Optimization of Bacillus subtilis as an expression system |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Patent granted |
Ref document number: 120741 Country of ref document: FI |
|
MM | Patent lapsed |