FI120741B - DNA:n vahvistaminen - Google Patents

DNA:n vahvistaminen Download PDF

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Publication number
FI120741B
FI120741B FI953079A FI953079A FI120741B FI 120741 B FI120741 B FI 120741B FI 953079 A FI953079 A FI 953079A FI 953079 A FI953079 A FI 953079A FI 120741 B FI120741 B FI 120741B
Authority
FI
Finland
Prior art keywords
sequence
dna
dna sequence
cell
homologous
Prior art date
Application number
FI953079A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI953079A0 (fi
FI953079A (fi
Inventor
Steen Troels Joergensen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of FI953079A0 publication Critical patent/FI953079A0/fi
Publication of FI953079A publication Critical patent/FI953079A/fi
Application granted granted Critical
Publication of FI120741B publication Critical patent/FI120741B/fi

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Semiconductor Lasers (AREA)
FI953079A 1992-12-22 1995-06-21 DNA:n vahvistaminen FI120741B (fi)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DK921539A DK153992D0 (da) 1992-12-22 1992-12-22 Metode
DK153992 1992-12-22
PCT/DK1993/000438 WO1994014968A1 (en) 1992-12-22 1993-12-22 Dna amplification
DK9300438 1993-12-22
CN94109005A CN1063488C (zh) 1992-12-22 1994-06-22 Dna扩增
CN94109005 1994-06-22

Publications (3)

Publication Number Publication Date
FI953079A0 FI953079A0 (fi) 1995-06-21
FI953079A FI953079A (fi) 1995-06-21
FI120741B true FI120741B (fi) 2010-02-15

Family

ID=37075831

Family Applications (1)

Application Number Title Priority Date Filing Date
FI953079A FI120741B (fi) 1992-12-22 1995-06-21 DNA:n vahvistaminen

Country Status (11)

Country Link
EP (1) EP0675959B1 (zh)
JP (1) JP3983798B2 (zh)
CN (1) CN1063488C (zh)
AT (1) ATE323167T1 (zh)
AU (1) AU679707B2 (zh)
CA (1) CA2152371C (zh)
DE (1) DE69334006T2 (zh)
DK (2) DK153992D0 (zh)
FI (1) FI120741B (zh)
NO (1) NO952496L (zh)
WO (1) WO1994014968A1 (zh)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR960701200A (ko) * 1993-03-15 1996-02-24 스티븐 에이. 쉐어윈 Dna의 예정-결실 방법(method for defined deletions of dna)
EP0817856A1 (en) * 1995-03-22 1998-01-14 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
FR2762842B1 (fr) 1997-05-05 1999-07-23 Pasteur Institut Procede de detection de la presence de matieres biologiques d'origine bovine, et oligonucleotides pour sa mise en oeuvre
CN1261918A (zh) * 1997-07-03 2000-08-02 诺沃挪第克公司 具有多拷贝目的基因和可筛选标记而不具选择性标记的产蛋白质细胞
AU753578B2 (en) 1997-12-22 2002-10-24 Novozymes A/S Carbohydrate oxidase and use thereof in baking
JP2004501651A (ja) 2000-06-23 2004-01-22 ノボザイムス　アクティーゼルスカブ 遺伝子の安定した染色体多コピー組み込みのための方法
AU2003214036A1 (en) 2002-04-10 2003-10-27 Novozymes A/S Bacillus licheniformis mutant host cell
CN1659283A (zh) 2002-04-10 2005-08-24 诺维信公司 地衣芽孢杆菌的突变宿主细胞
WO2005042750A1 (en) 2003-10-31 2005-05-12 Novozymes A/S Method for stable gene-amplification in a bacterial host cell
EP1707626A4 (en) * 2003-11-25 2008-07-23 Japan Science & Tech Agency gene amplification
WO2005123915A1 (en) 2004-06-21 2005-12-29 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
JP4821012B2 (ja) * 2005-11-24 2011-11-24 大学共同利用機関法人自然科学研究機構 遺伝子増幅法
CN103255114B (zh) 2006-11-29 2015-03-25 诺维信股份有限公司 改进向细菌细胞中导入dna的方法
CN101611145B (zh) 2006-12-21 2013-08-14 诺维信股份有限公司 用于在细菌细胞中表达基因的修饰型信使rna稳定化序列
WO2010097436A1 (en) 2009-02-27 2010-09-02 Novozymes A/S Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production
EP2516636A1 (en) 2009-12-21 2012-10-31 Novozymes Inc. Methods for producing heterologous polypeptides in thiol-disulfide oxidoreductase-deficient bacterial mutant cells
US20140141471A1 (en) 2011-05-19 2014-05-22 Novozymes, Inc. Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins
EP3532619A1 (en) 2016-10-25 2019-09-04 Novozymes A/S Flp-mediated genomic integration
CN107287331B (zh) * 2017-08-01 2020-06-26 北京大学 一种高效检测蓝藻细胞周期的方法
CN111511908A (zh) 2017-11-10 2020-08-07 诺维信公司 温度敏感性cas9蛋白
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
CN113366113A (zh) 2019-01-30 2021-09-07 诺维信公司 同源折叠酶共表达
EP3935160A1 (en) 2019-03-08 2022-01-12 Novozymes A/S Means and methods for improving protease expression
CN113939588A (zh) 2019-05-15 2022-01-14 诺维信公司 温度敏感性的rna指导的内切核酸酶
EP3990629A1 (en) 2019-06-25 2022-05-04 Novozymes A/S Counter-selection by inhibition of conditionally essential genes
EP4370534A1 (en) 2021-07-13 2024-05-22 Novozymes A/S Recombinant cutinase expression
WO2023247514A1 (en) 2022-06-22 2023-12-28 Novozymes A/S Recombinant mannanase expression

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
AU620326B2 (en) * 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
DK0779362T4 (da) * 1989-12-22 2012-07-30 Serono Lab DNA-konstruktioner til aktivering og modifikation af endogene geners ekspression

Also Published As

Publication number Publication date
EP0675959B1 (en) 2006-04-12
NO952496D0 (no) 1995-06-21
FI953079A0 (fi) 1995-06-21
NO952496L (no) 1995-08-21
CN1114355A (zh) 1996-01-03
DK0675959T3 (da) 2006-08-07
AU5694494A (en) 1994-07-19
CN1063488C (zh) 2001-03-21
CA2152371C (en) 2008-12-02
JP3983798B2 (ja) 2007-09-26
DE69334006T2 (de) 2006-11-23
ATE323167T1 (de) 2006-04-15
DE69334006D1 (de) 2006-05-24
WO1994014968A1 (en) 1994-07-07
FI953079A (fi) 1995-06-21
DK153992D0 (da) 1992-12-22
CA2152371A1 (en) 1994-07-07
AU679707B2 (en) 1997-07-10
JPH08504585A (ja) 1996-05-21
EP0675959A1 (en) 1995-10-11

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