FI113665B - Konstruerat DNA, detta innehållande expressionsvektor och cell samt förfarande för framställning av serin och serinbaserade produkter - Google Patents
Konstruerat DNA, detta innehållande expressionsvektor och cell samt förfarande för framställning av serin och serinbaserade produkter Download PDFInfo
- Publication number
- FI113665B FI113665B FI942711A FI942711A FI113665B FI 113665 B FI113665 B FI 113665B FI 942711 A FI942711 A FI 942711A FI 942711 A FI942711 A FI 942711A FI 113665 B FI113665 B FI 113665B
- Authority
- FI
- Finland
- Prior art keywords
- pgd
- serine
- dna
- aeqg
- wild
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Ceramic Products (AREA)
- Tires In General (AREA)
- Epoxy Resins (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (12)
1. Konstruerat DNA, som kodar 3-fosfoglyceratdehydrogenas (PGD) med i jämförel-se med vildtyps-PGD nedsatt sensitivitet mot av serin förorsakad inhibition, känne- 5 tecknad därav, att nämnda DNA kodar PGD, som innehäller en förändring i C-terminalen till 25 % av vildtyps-PGD.
2. Konstruerat DNA enligt patentkravet 1, kännetecknad därav, att DNA kodar PGD med en förändring i vildtyps-PGD:s C-terminala 50 aminosyror. 10
3. Konstruerat DNA enligt patentkravet 1, kännetecknad därav, att DNA kodar PGD med en C-terminal deletion av vild-typs-PGD.
4. Konstruerat DNA enligt patentkravet 1, kännetecknad därav, att den C-15 terminala förändringen är insertion i vild-typs-PGD:s sekvenssiin.
5. Konstruerat DNA enligt patentkravet 4, kännetecknad därav, att insertionen är mellan vild-typs-PGD:s VAL 363 och ASN 364 eller mellan ALA 392 och GLN 394.
6. Konstruerat DNA enligt patentkravet 1, kännetecknad därav, att DNA kodar • PGD med nägon av aminosyrasekvenserna; . : AEQG V— -NIA AQYL QTSA QMGY WID IEAD EDVAEKAL --QA MKAI PASL D; .., AEQG VLV; '1 AEQG VL; •V*: 25 AEQG VCSR ΑΝΙΑ AQYL OTSA QMGY WID IEAD EDVAEKAL-QA MKAI PGT- -IRA RLLY; AEQG V— -NIA AQYL QTSA QMGY WID IEAD EDVAEKAL SRQA MKAI PGT- -IRA RLLY; AEQG V— -NIA AQYL QTSA QMGY WID IEAD EDVAEKAL L; AEQG V— -NIA AQYL QTSA QMGY WID IEAD EDVAEKAL -QA MKAI PGT- AIR RLLY; AEQG V— -NIA AQYL QTSA QMGY WID IEAD EDVAEKAL -QA MKAI PVL, • · · ·”’· 30 AEQG V............... — -.............- .............CSR AIRA RLLY; AEQG V— -NIA AQYL QTSA QMGY WID IEAD EDVAEKAL.............SR AIRA RLLY; • · * • » » • · • · : * * ’: i stället för vild-typs-PGD:s 52 C-terminala aminosyra. • · · * · · • · ·
7. Fosfoglyceratdehydrogenas med en aminosyrasekvens kodad av ett konstruerat • * DNA enligt nägot av patentkraven 1-6. i9 1 13665
8. Expressionsgvektor, kännetecknad därav, att den omfattar ett konstruerat DNA enligt nägot av patentkraven 1-6 och ett regulator-DNA positionerad och oriente-rad s§, att se den är lämpad för expression av det konstruerade DNA i ett värdex- 5 pressionssystem.
9. Cell, kännetecknad därav, att den omfatta ett konstruerat DNA enligt nägot av patentkraven 1 - 6 ett regulator-DNA positionerad och orienterad sä, att det konstruerade DNA expresseras i nämnda cell. 10
10. Cell enligt patentkravet 9, kännetecknad därav, att i nämnda cell har serA av vildtyp deleterats.
11. Förfarande för produktion av önskad produkt, som utgörs av serin eller en se-15 rinbaserad produkt, kännetecknat därav, att en cell enligt patentkravet 9 odlas och den önskade produkten tillvaratas.
12. Förfarande enligt patentkravet 11, kännetecknat därav, att produkten är serin.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP91121385 | 1991-12-12 | ||
| EP91121385 | 1991-12-12 | ||
| EP9201873 | 1992-08-17 | ||
| PCT/EP1992/001873 WO1993012235A1 (en) | 1991-12-12 | 1992-08-17 | Materials and methods for biosynthesis of serine and serine-related products |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI942711A FI942711A (sv) | 1994-06-09 |
| FI942711A0 FI942711A0 (sv) | 1994-06-09 |
| FI113665B true FI113665B (sv) | 2004-05-31 |
Family
ID=8207424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI942711A FI113665B (sv) | 1991-12-12 | 1994-06-09 | Konstruerat DNA, detta innehållande expressionsvektor och cell samt förfarande för framställning av serin och serinbaserade produkter |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US5618716A (sv) |
| EP (1) | EP0620853B1 (sv) |
| JP (1) | JP2584409B2 (sv) |
| KR (1) | KR0132258B1 (sv) |
| CN (1) | CN1065914C (sv) |
| AU (1) | AU668359B2 (sv) |
| BR (1) | BR9206902A (sv) |
| CA (1) | CA2124214C (sv) |
| CZ (1) | CZ285915B6 (sv) |
| DE (1) | DE69208894T2 (sv) |
| ES (1) | ES2084373T3 (sv) |
| FI (1) | FI113665B (sv) |
| HU (1) | HU217795B (sv) |
| RU (1) | RU2154671C1 (sv) |
| SK (1) | SK279805B6 (sv) |
| TW (1) | TW313589B (sv) |
| UA (1) | UA39861C2 (sv) |
| WO (1) | WO1993012235A1 (sv) |
| ZA (1) | ZA926405B (sv) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4232468A1 (de) * | 1992-09-28 | 1994-03-31 | Consortium Elektrochem Ind | Mikroorganismen für die Produktion von Tryptophan und Verfahren zu ihrer Herstellung |
| JP4066543B2 (ja) * | 1998-01-12 | 2008-03-26 | 味の素株式会社 | 発酵法によるｌ−セリンの製造法 |
| JP3997631B2 (ja) * | 1998-01-12 | 2007-10-24 | 味の素株式会社 | 発酵法によるｌ−セリンの製造法 |
| US6431870B1 (en) | 1999-11-30 | 2002-08-13 | Ora Metrix, Inc. | Method and apparatus for generating a desired three-dimensional digital model of an orthodontic structure |
| US6350120B1 (en) | 1999-11-30 | 2002-02-26 | Orametrix, Inc. | Method and apparatus for designing an orthodontic apparatus to provide tooth movement |
| US6540512B1 (en) | 1999-11-30 | 2003-04-01 | Orametrix, Inc. | Method and apparatus for treating an orthodontic patient |
| US6471512B1 (en) | 1999-11-30 | 2002-10-29 | Ora Metrix, Inc. | Method and apparatus for determining and monitoring orthodontic treatment |
| US6250918B1 (en) | 1999-11-30 | 2001-06-26 | Orametrix, Inc. | Method and apparatus for simulating tooth movement for an orthodontic patient |
| US6509191B2 (en) * | 2000-05-02 | 2003-01-21 | Alex Liu | Identification and characterization of a PAGODA phenotype (PGD) in plants |
| DE10331291A1 (de) | 2003-07-10 | 2005-02-17 | Consortium für elektrochemische Industrie GmbH | Varianten der 3-Phosphoglyceratdehydrogenase mit reduzierter Hemmung durch L-Serin und dafür codierende Gene |
| EP1871274B1 (en) | 2005-03-07 | 2019-05-08 | Align Technology, Inc. | Wrinkled dental aligner |
| US20060275731A1 (en) | 2005-04-29 | 2006-12-07 | Orthoclear Holdings, Inc. | Treatment of teeth by aligners |
| DE102005049527B4 (de) * | 2005-10-17 | 2013-05-08 | Forschungszentrum Jülich GmbH | Verfahren zur Herstellung von L-Serin, Gensequenz, Vektoren sowie Mikroorganismus |
| RU2006129690A (ru) | 2006-08-16 | 2008-02-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА ydiN, ГЕНА ydiB ИЛИ ИХ КОМБИНАЦИИ |
| JP2010017081A (ja) * | 2006-10-10 | 2010-01-28 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| JP2010017082A (ja) * | 2006-10-10 | 2010-01-28 | Ajinomoto Co Inc | Ｌ−アミノ酸の製造法 |
| BRPI0816429A2 (pt) | 2007-09-04 | 2019-09-24 | Ajinomoto Kk | microorganismo, e, método para a produção de um l-aminoácido. |
| EP2138585B1 (en) * | 2008-03-06 | 2011-02-09 | Ajinomoto Co., Inc. | An L-cysteine producing bacterium and a method for producing L-cysteine |
| JP5332237B2 (ja) * | 2008-03-06 | 2013-11-06 | 味の素株式会社 | Ｌ−システイン生産菌及びｌ−システインの製造法 |
| MX2011007039A (es) | 2008-12-31 | 2011-07-20 | Metabolic Explorer Sa | Metodo para la preparacion de dioles. |
| WO2011065469A1 (ja) | 2009-11-30 | 2011-06-03 | 味の素株式会社 | Ｌ－システイン生産菌及びｌ－システインの製造法 |
| US8911978B2 (en) | 2010-07-02 | 2014-12-16 | Metabolic Explorer | Method for the preparation of hydroxy acids |
| DE102011075656A1 (de) | 2011-05-11 | 2012-03-29 | Wacker Chemie Ag | Verfahren zur fermentativen Produktion von L-Cystin |
| DE102011078481A1 (de) | 2011-06-30 | 2013-01-03 | Wacker Chemie Ag | Verfahren zur fermentativen Produktion von natürlichem L-Cystein |
| DE102012208359A1 (de) | 2012-05-18 | 2013-11-21 | Wacker Chemie Ag | Verfahren zur fermentativen Produktion von L-Cystein und Derivaten dieser Aminosäure |
| DE102012216527A1 (de) | 2012-09-17 | 2014-03-20 | Wacker Chemie Ag | Verfahren zur fermentativen Produktion von L-Cystein und Derivaten dieser Aminosäure |
| WO2014049382A2 (en) | 2012-09-26 | 2014-04-03 | Metabolic Explorer | Ethylenediamine fermentative production by a recombinant microorganism |
| DE102013209274A1 (de) | 2013-05-17 | 2014-11-20 | Wacker Chemie Ag | Mikroorganismus und Verfahren zur fermentativen Überproduktion von Gamma-Glutamylcystein und Derivaten dieses Dipeptids |
| JP7463388B2 (ja) | 2019-02-20 | 2024-04-08 | ブラスケム エス．エー． | ペントース糖およびヘキソース糖のための分解経路 |
| JP7504112B2 (ja) | 2019-02-20 | 2024-06-21 | ブラスケム エス．エー． | ヘキソースからの含酸素化合物の生成のための微生物および方法 |
| US20230265473A1 (en) | 2020-06-26 | 2023-08-24 | Wacker Chemie Ag | Improved cysteine-producing strains |
| WO2023165684A1 (de) | 2022-03-01 | 2023-09-07 | Wacker Chemie Ag | Verbesserte cystein produzierende stämme |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0190921A3 (en) * | 1985-02-04 | 1988-01-13 | Engenics, Inc. | Method for the overproduction of amino acids |
| US4743546A (en) * | 1985-02-13 | 1988-05-10 | Biotechnica International, Inc. | Controlled gene excision |
| US4753883A (en) * | 1986-05-07 | 1988-06-28 | Biotechnica International, Inc. | Enzyme deregulation |
| US4753833A (en) * | 1986-09-26 | 1988-06-28 | Fishgal Semyon I | Hollow article with zigzag projections |
| GB8828806D0 (en) * | 1988-12-09 | 1989-01-18 | Beecham Group Plc | Novel compounds |
| JP2967996B2 (ja) * | 1989-06-06 | 1999-10-25 | 協和醗酵工業株式会社 | Ｌ―トリプトファンの製造法 |
| US5120837A (en) * | 1989-09-20 | 1992-06-09 | The Nutrasweet Company | Dna encoding phe a feedback inhibition resistant enzyme analogues |
-
1992
- 1992-03-24 TW TW081102201A patent/TW313589B/zh active
- 1992-08-17 ES ES92917826T patent/ES2084373T3/es not_active Expired - Lifetime
- 1992-08-17 BR BR9206902A patent/BR9206902A/pt not_active Application Discontinuation
- 1992-08-17 EP EP92917826A patent/EP0620853B1/en not_active Expired - Lifetime
- 1992-08-17 CZ CZ941406A patent/CZ285915B6/cs not_active IP Right Cessation
- 1992-08-17 UA UA94005362A patent/UA39861C2/uk unknown
- 1992-08-17 WO PCT/EP1992/001873 patent/WO1993012235A1/en active IP Right Grant
- 1992-08-17 RU RU94030817/13A patent/RU2154671C1/ru active
- 1992-08-17 KR KR1019940701948A patent/KR0132258B1/ko active
- 1992-08-17 DE DE69208894T patent/DE69208894T2/de not_active Expired - Lifetime
- 1992-08-17 HU HU9400796A patent/HU217795B/hu unknown
- 1992-08-17 US US08/244,491 patent/US5618716A/en not_active Expired - Lifetime
- 1992-08-17 CA CA002124214A patent/CA2124214C/en not_active Expired - Lifetime
- 1992-08-17 AU AU24268/92A patent/AU668359B2/en not_active Expired
- 1992-08-17 JP JP5510540A patent/JP2584409B2/ja not_active Expired - Lifetime
- 1992-08-17 SK SK700-94A patent/SK279805B6/sk not_active IP Right Cessation
- 1992-08-25 ZA ZA926405A patent/ZA926405B/xx unknown
- 1992-10-10 CN CN92112014A patent/CN1065914C/zh not_active Expired - Lifetime
-
1994
- 1994-06-09 FI FI942711A patent/FI113665B/sv not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| HUT69802A (en) | 1995-09-28 |
| RU2154671C1 (ru) | 2000-08-20 |
| DE69208894T2 (de) | 1996-07-18 |
| ES2084373T3 (es) | 1996-05-01 |
| AU668359B2 (en) | 1996-05-02 |
| ZA926405B (en) | 1993-04-28 |
| CZ140694A3 (en) | 1995-01-18 |
| CN1065914C (zh) | 2001-05-16 |
| HU9400796D0 (en) | 1994-06-28 |
| CZ285915B6 (cs) | 1999-11-17 |
| JP2584409B2 (ja) | 1997-02-26 |
| FI942711A (sv) | 1994-06-09 |
| EP0620853B1 (en) | 1996-03-06 |
| DE69208894D1 (de) | 1996-04-11 |
| JPH06510911A (ja) | 1994-12-08 |
| WO1993012235A1 (en) | 1993-06-24 |
| CA2124214C (en) | 2002-04-02 |
| AU2426892A (en) | 1993-07-19 |
| HU217795B (hu) | 2000-04-28 |
| BR9206902A (pt) | 1995-11-21 |
| US5618716A (en) | 1997-04-08 |
| UA39861C2 (uk) | 2001-07-16 |
| FI942711A0 (sv) | 1994-06-09 |
| KR0132258B1 (ko) | 1998-04-11 |
| CA2124214A1 (en) | 1993-06-24 |
| TW313589B (sv) | 1997-08-21 |
| SK70094A3 (en) | 1995-03-08 |
| CN1073209A (zh) | 1993-06-16 |
| SK279805B6 (sk) | 1999-04-13 |
| EP0620853A1 (en) | 1994-10-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MA | Patent expired |