FI107807B - Hydrolys av stärkelse till glukos med hjälp av ett genmanipulerat enzym - Google Patents
Hydrolys av stärkelse till glukos med hjälp av ett genmanipulerat enzym Download PDFInfo
- Publication number
- FI107807B FI107807B FI925901A FI925901A FI107807B FI 107807 B FI107807 B FI 107807B FI 925901 A FI925901 A FI 925901A FI 925901 A FI925901 A FI 925901A FI 107807 B FI107807 B FI 107807B
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- FI
- Finland
- Prior art keywords
- glucoamylase
- amino acid
- glucose
- enzyme
- exchange
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- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
Claims (9)
1. Förfarande för omvandling av stärkelse eller partiellt hydrolyserad stärkelse tili en sirap innehällande dextros, kännetecknat av att det innefattar steget att ett hydroly-sat av stärkelse försockras i närvaro av en mutant av ett 15 glukoamylas, som kan erhällas frän en stam av Aspergillus, vilken i förhällande tili glukoamylas frän Aspergillus niqer uppvisar ökad selektivitet för a- (1__>4) -glukosidbind- ningar, och vilken innefattar nägon av följande mutationer: 20 utbyte av aminosyran i den ställning som motsvarar Serll9 i glukoamylas frän A. niaer mot en aminosyra skild frän Ser, • · ... företrädesvis Tyr; • · utbyte av aminosyran i den ställning som motsvarar Asnl82 i » · i j·’ * glukoamylas frän A. niaer mot en aminosyra skild frän Asn, : 25 företrädesvis Ala; • · utbyte av aminosyran i den ställning som motsvarar Glyl83 i • · · ·/· · glukoamylas frän A. niaer mot en aminosyra skild frän Gly, företrädesvis Lys; ····· utbyte av aminosyran i den ställning som motsvarar Serl84 i .**. 30 glukoamylas frän A. niaer mot en aminosyra skild frän Ser, • företrädesvis His. * • · · • · · ·· · • · ·
2. Förfarande enligt patentkrav 1, kännetecknat av att det ··· muterade glukoamylaset är härlett frän ett glukoamylas som • · « · .···. 35 kan erhällas frän A. niaer eller A. awamori. • · «n 107807
3. Förfarande enligt patentkrav 1 eller 2, kännetecknat av att det muterade glukoamylaset innefattar tvä eller flera av aiainosyrasubstitutionerna.
4. Förfarande enligt nägot av patentkraven 1 tili 3, kän-5 netecknat av att dosen av glukoamylas är 0,05 tili 0,5 AG- enheter per gram torrt fast material.
5. Förfarande enligt nägot av föregäende patentkrav, kännetecknat av att det innefattar försockring av ett stärkel- 10 sehydrolysat i en koncentration av minst 30 viktprocent torrt fast material.
6. Förfarande enligt nägot av föregäende patentkrav, kännetecknat av att försockringen genomförs i närvaro av ett 15 enzym för avlägsnande av grenar, valt bland pullulanaser och isoamylaser, företrädesvis ett pullulanas härlett frän Bacillus acidopullulyticus eller ett isoamylas härlett frän Pseudomonas amyloderamosa. 20
7. Förfarande enligt nägot av föregäende patentkrav, kän netecknat av att försockringen genomförs vid ett pH av 3 tili 5,5 och en temperatur av 30 tili 60 °C under 48 till 72 timmar, företrädesvis vid ett pH av 4 tili 4,5 och en :*·*: temperatur av 55 tili 60 °C. 25
• · · · : 8. Mutant av ett glukoamylas som kan erhällas frän en stam • · · • · av Aspergillus, kännetecknad av att den i förhällande tili • · · glukoamylas frän Aspecraillus niger uppvisar ökad selektivi- . tet för a- (1_»4) -glukosidbindningar, och vilken innefattar [ 30 nägon av följande mutationer: » » • · · utbyte av aminosyran i den ställning som motsvarar Serll9 i ♦ ♦ · glukoamylas frän A. niger mot en aminosyra skild frän Ser, • · · företrädesvis Tyr; • · · •••j 35 utbyte av aminosyran i den ställning som motsvarar Asnl82 i • « *···’ glukoamylas frän A. niger mot en aminosyra skild frän Asn, företrädesvis Ala; 107807 utbyte av aminosyran i den ställning som motsvarar Glyl83 i glukoamylas frän A. niger mot en aminosyra skild frän Gly, företrädesvis Lys; utbyte av aminosyran i den ställning som motsvarar Serl84 i 5 glukoamylas frän A. niner mot en aminosyra skild frän Ser, företrädesvis His, med det förbehället att när det muterade enzymet är härlett frän glukoamylas frän A. nicrer är utbyte av Asn mot Ala i aminosyraställningen Asnl82 inte den enda mutationen. 10
9. Mutant av ett glukoamylas enligt patentkrav 8, känne-tecknad av att den innefattar tvä eller flera av aminosyra-substitutionerna. • · · · • · ··· » · • · • ·· • · · • · · • · · « • · • · * ♦ · · • · · · • · • · · • ·· • · • · · • · · ♦ * ♦ « » · « ·* • · «· · • · · • « · »·· ·#· • · • · • · · ·«· « · · · M* « · ···
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/546,511 US5162210A (en) | 1990-06-29 | 1990-06-29 | Process for enzymatic hydrolysis of starch to glucose |
| US54651190 | 1990-06-29 | ||
| DK9100182 | 1991-06-28 | ||
| PCT/DK1991/000182 WO1992000381A1 (en) | 1990-06-29 | 1991-06-28 | Enzymatic hydrolysis of starch to glucose, using a genetically engineered enzyme |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI925901A0 FI925901A0 (fi) | 1992-12-28 |
| FI925901A FI925901A (fi) | 1992-12-28 |
| FI107807B true FI107807B (sv) | 2001-10-15 |
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ID=24180763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI925901A FI107807B (sv) | 1990-06-29 | 1992-12-28 | Hydrolys av stärkelse till glukos med hjälp av ett genmanipulerat enzym |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5162210A (sv) |
| EP (1) | EP0536270B1 (sv) |
| JP (1) | JP3249514B2 (sv) |
| AT (1) | ATE195972T1 (sv) |
| DE (1) | DE69132391T2 (sv) |
| DK (1) | DK0536270T3 (sv) |
| ES (1) | ES2150905T3 (sv) |
| FI (1) | FI107807B (sv) |
| GR (1) | GR3034781T3 (sv) |
| WO (1) | WO1992000381A1 (sv) |
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| BR112020004476A2 (pt) | 2017-09-15 | 2020-09-08 | Novozymes A/S | processo para produção de um produto de fermentação, e, uso de uma combinação de enzimas |
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| CA3143527A1 (en) | 2019-08-06 | 2021-02-11 | Novozymes A/S | Fusion proteins for improved enzyme expression |
| WO2021046073A1 (en) | 2019-09-05 | 2021-03-11 | Dupont Nutrition Biosciences Aps | Feed composition |
| CA3152952A1 (en) | 2019-09-16 | 2021-03-25 | Novozymes A/S | Polypeptides having beta-glucanase activity and polynucleotides encoding same |
| BR112022011271A2 (pt) | 2019-12-10 | 2022-09-06 | Novozymes As | Célula hospedeira recombinante, composição, métodos para produzir um derivado de uma célula e um produto de fermentação, e, uso de uma célula hospedeira recombinante |
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| EP4103709A2 (en) | 2020-02-10 | 2022-12-21 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
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| AU2022288057A1 (en) | 2021-06-07 | 2023-12-21 | Novozymes A/S | Engineered microorganism for improved ethanol fermentation |
| CA3232987A1 (en) | 2021-09-27 | 2023-03-30 | Marion BERNARDEAU | Feed additive compositions and methods for using the same |
| WO2023225510A1 (en) | 2022-05-17 | 2023-11-23 | Dupont Nutrition Biosciences Aps | Feed additive comprising enzyme combinations |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4560651A (en) * | 1981-04-20 | 1985-12-24 | Novo Industri A/S | Debranching enzyme product, preparation and use thereof |
| CA1211314A (en) * | 1983-05-20 | 1986-09-16 | William F. Line | Production of dextrose and maltose syrups using an enzyme derived from rice |
| FI82711C (sv) * | 1983-09-11 | 1991-04-10 | Gist Brocades Nv | Ny enzymprodukt och dess användning vid försockring av stärkelse |
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1990
- 1990-06-29 US US07/546,511 patent/US5162210A/en not_active Expired - Fee Related
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1991
- 1991-06-28 WO PCT/DK1991/000182 patent/WO1992000381A1/en active IP Right Grant
- 1991-06-28 ES ES91912567T patent/ES2150905T3/es not_active Expired - Lifetime
- 1991-06-28 AT AT91912567T patent/ATE195972T1/de not_active IP Right Cessation
- 1991-06-28 DE DE69132391T patent/DE69132391T2/de not_active Expired - Fee Related
- 1991-06-28 JP JP51161491A patent/JP3249514B2/ja not_active Expired - Fee Related
- 1991-06-28 DK DK91912567T patent/DK0536270T3/da active
- 1991-06-28 EP EP91912567A patent/EP0536270B1/en not_active Expired - Lifetime
-
1992
- 1992-12-28 FI FI925901A patent/FI107807B/sv active
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2000
- 2000-11-08 GR GR20000402470T patent/GR3034781T3/el unknown
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|---|---|
| DK0536270T3 (da) | 2000-10-30 |
| FI925901A0 (fi) | 1992-12-28 |
| FI925901A (fi) | 1992-12-28 |
| GR3034781T3 (en) | 2001-02-28 |
| EP0536270A1 (en) | 1993-04-14 |
| ES2150905T3 (es) | 2000-12-16 |
| JPH05507852A (ja) | 1993-11-11 |
| DE69132391D1 (de) | 2000-10-05 |
| WO1992000381A1 (en) | 1992-01-09 |
| DE69132391T2 (de) | 2001-02-15 |
| ATE195972T1 (de) | 2000-09-15 |
| US5162210A (en) | 1992-11-10 |
| JP3249514B2 (ja) | 2002-01-21 |
| EP0536270B1 (en) | 2000-08-30 |
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