ES8802399A1 - Un procedimiento para la preparacion de un plasmido que se replica autonomamente en e. coli. - Google Patents

Un procedimiento para la preparacion de un plasmido que se replica autonomamente en e. coli.

Info

Publication number
ES8802399A1
ES8802399A1 ES548940A ES548940A ES8802399A1 ES 8802399 A1 ES8802399 A1 ES 8802399A1 ES 548940 A ES548940 A ES 548940A ES 548940 A ES548940 A ES 548940A ES 8802399 A1 ES8802399 A1 ES 8802399A1
Authority
ES
Spain
Prior art keywords
lipolytica
vectors
pld25
cloning
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES548940A
Other languages
English (en)
Other versions
ES548940A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Inc
Original Assignee
Pfizer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Inc filed Critical Pfizer Inc
Publication of ES548940A0 publication Critical patent/ES548940A0/es
Publication of ES8802399A1 publication Critical patent/ES8802399A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Magnetic Ceramics (AREA)
  • Treatment Of Sludge (AREA)
  • Electrotherapy Devices (AREA)
  • Protection Of Transformers (AREA)
  • Separation By Low-Temperature Treatments (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Air-Conditioning For Vehicles (AREA)
  • Saccharide Compounds (AREA)
  • Paper (AREA)
  • Blow-Moulding Or Thermoforming Of Plastics Or The Like (AREA)

Abstract

PROCEDIMIENTO PARA LA PREPARACION DE UN PLASMIDO QUE SE REPLICA AUTONOMAMENTE EN E. COLI Y SE INTEGRA PERO NO SE REPLICA AUTONOMAMENTE EN YARRONIA LIPOLYTICA, AL CONTENER UN FRAGMENTO DE DNA DE Y. LIPLITICA QUE COMPLEMENTA LAS MUTACIONES LEUB DE E. COLI Y CONTENER EL GEN LEU2 DE Y. LIPOLITICA. CONTIENE TAMBIEN LOS SITIOS 3ECORU, 8AVAI, ISPHI, 1KPNI, 2SALI, 4ECORI, 2XHOI Y 2BGLIII Y CARECE DE LOS SITIOS DE RESTRICCION CON ENDONUCLEASAS MIND III, CLAI, BAMHI Y N2MI. CONSISTENTE EN LISAR UN PLASMIDO DE E. COLI LINEARIZADO CON FRAGMENTOS DE DNA DE Y. LIPOLYTICA COMPRENDIENDO DICHO DNA, UNA REGION DE HOMOLOGIA AL DNA DE Y. LIPOLYTICA Y DE UN MARCARDOR GENETICO DETECTABLE, PREFERENTEMENTE EL GEN LEU2. DE APLICACION EN LA CREACION DE VECTORES DE CLONAJE PARA LA TRANSFORMACION DE YARRONIA LIPOLYTICA.
ES548940A 1983-10-06 1985-11-15 Un procedimiento para la preparacion de un plasmido que se replica autonomamente en e. coli. Expired ES8802399A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US53959183A 1983-10-06 1983-10-06

Publications (2)

Publication Number Publication Date
ES548940A0 ES548940A0 (es) 1988-05-16
ES8802399A1 true ES8802399A1 (es) 1988-05-16

Family

ID=24151875

Family Applications (3)

Application Number Title Priority Date Filing Date
ES536546A Expired ES8605038A1 (es) 1983-10-06 1984-10-05 Un procedimiento para la transformacion de yarrowia lipolytica
ES548940A Expired ES8802399A1 (es) 1983-10-06 1985-11-15 Un procedimiento para la preparacion de un plasmido que se replica autonomamente en e. coli.
ES548941A Expired ES8900255A1 (es) 1983-10-06 1985-11-15 Un procedimiento de clonado de genes por complementacion de mutantes en y. lipolytica

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES536546A Expired ES8605038A1 (es) 1983-10-06 1984-10-05 Un procedimiento para la transformacion de yarrowia lipolytica

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES548941A Expired ES8900255A1 (es) 1983-10-06 1985-11-15 Un procedimiento de clonado de genes por complementacion de mutantes en y. lipolytica

Country Status (17)

Country Link
EP (1) EP0138508B1 (es)
JP (2) JP2559689B2 (es)
KR (1) KR870000239B1 (es)
AT (1) ATE73848T1 (es)
AU (1) AU554118B2 (es)
BR (1) BR8404984A (es)
DE (1) DE3485589D1 (es)
DK (2) DK171878B1 (es)
ES (3) ES8605038A1 (es)
FI (1) FI85987C (es)
IE (1) IE57685B1 (es)
IL (1) IL73119A (es)
IN (1) IN163393B (es)
NO (1) NO173743C (es)
NZ (1) NZ209794A (es)
PH (1) PH25363A (es)
ZA (1) ZA847824B (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2579617B2 (fr) * 1985-04-02 1987-06-26 Agronomique Inst Nat Rech Vecteurs de transformation de la levure yarrowia lipolytica, procede de transformation et levure transformee
EP0166659B1 (fr) * 1984-06-26 1989-08-30 Institut National De La Recherche Agronomique (Inra) Vecteurs de transformation de la levure yarrowia lipolytica, procédé de transformation et levure transformée
US4937189A (en) * 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
GB8529275D0 (en) * 1985-11-28 1986-01-02 Whitbread & Co Plc Dna recombination
JPH082304B2 (ja) * 1987-12-17 1996-01-17 オリエンタル酵母工業株式会社 プラスミドとその製造方法
FR2626584B1 (fr) * 1988-01-28 1990-07-13 Agronomique Inst Nat Rech Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation
FR2734843A1 (fr) * 1995-06-02 1996-12-06 Centre Nat Rech Scient Nouveau procede de bioconversion microbienne.
EP0747484A1 (en) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Upstream activator sequences and recombinant promoter sequences functional in yarrowia and vectors containing them
GB9715905D0 (en) * 1997-07-28 1997-10-01 Ciba Geigy Ag Plant phosphoribosyl transferase and DNA coding thereof
FR2782733B1 (fr) * 1998-09-01 2002-02-08 Agronomique Inst Nat Rech Procede de transformation non-homologue de yarrowia lipolytica
JP4554370B2 (ja) * 2002-03-29 2010-09-29 ゾーマ テクノロジー リミテッド 多重遺伝子プラスミドおよび組み換えポリペプチドの発現増加法
EP2371967B1 (en) 2005-03-18 2015-06-03 DSM IP Assets B.V. Production of carotenoids in oleaginous yeast and fungi
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
KR20090058077A (ko) * 2007-12-04 2009-06-09 충북대학교 산학협력단 생물체 정보의 염색체 내 표지 방법 및 그 정보가 표지된생물체
FR2927089B1 (fr) 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
EP3260549A1 (en) * 2008-05-23 2017-12-27 Nucelis LLC Production of squalene using yeast
US20100298612A1 (en) 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols

Also Published As

Publication number Publication date
ES8900255A1 (es) 1989-05-01
KR850003167A (ko) 1985-06-13
AU554118B2 (en) 1986-08-07
ZA847824B (en) 1986-05-28
IL73119A (en) 1990-01-18
JPS60199386A (ja) 1985-10-08
ATE73848T1 (de) 1992-04-15
EP0138508B1 (en) 1992-03-18
ES548940A0 (es) 1988-05-16
FI843932A0 (fi) 1984-10-05
FI85987C (fi) 1992-06-25
BR8404984A (pt) 1985-08-20
ES536546A0 (es) 1986-02-16
NZ209794A (en) 1989-01-27
NO173743B (no) 1993-10-18
FI843932L (fi) 1985-04-07
DK477084D0 (da) 1984-10-05
DK171878B1 (da) 1997-07-28
KR870000239B1 (ko) 1987-02-18
DE3485589D1 (de) 1992-04-23
IE57685B1 (en) 1993-02-24
IE842535L (en) 1985-04-06
DK171879B1 (da) 1997-07-28
EP0138508A1 (en) 1985-04-24
JP2559689B2 (ja) 1996-12-04
JP2718659B2 (ja) 1998-02-25
DK477084A (da) 1985-05-15
AU3387684A (en) 1985-04-18
ES548941A0 (es) 1989-05-01
PH25363A (en) 1991-05-13
JPH09131176A (ja) 1997-05-20
DK148195A (da) 1995-12-28
IN163393B (es) 1988-09-17
FI85987B (fi) 1992-03-13
NO844001L (no) 1985-04-09
IL73119A0 (en) 1984-12-31
ES8605038A1 (es) 1986-02-16
NO173743C (no) 1994-01-26

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