ATE73848T1 - Verfahren zur transformation von yarrowia lipolytica. - Google Patents

Verfahren zur transformation von yarrowia lipolytica.

Info

Publication number
ATE73848T1
ATE73848T1 AT84306734T AT84306734T ATE73848T1 AT E73848 T1 ATE73848 T1 AT E73848T1 AT 84306734 T AT84306734 T AT 84306734T AT 84306734 T AT84306734 T AT 84306734T AT E73848 T1 ATE73848 T1 AT E73848T1
Authority
AT
Austria
Prior art keywords
lipolytica
vectors
pld25
cloning
dna
Prior art date
Application number
AT84306734T
Other languages
English (en)
Inventor
Lance Steven Davidow
John Robert Dezeeuw
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Application granted granted Critical
Publication of ATE73848T1 publication Critical patent/ATE73848T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Magnetic Ceramics (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Sludge (AREA)
  • Separation By Low-Temperature Treatments (AREA)
  • Protection Of Transformers (AREA)
  • Electrotherapy Devices (AREA)
  • Paper (AREA)
  • Blow-Moulding Or Thermoforming Of Plastics Or The Like (AREA)
  • Air-Conditioning For Vehicles (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
AT84306734T 1983-10-06 1984-10-03 Verfahren zur transformation von yarrowia lipolytica. ATE73848T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53959183A 1983-10-06 1983-10-06
EP84306734A EP0138508B1 (de) 1983-10-06 1984-10-03 Verfahren zur Transformation von Yarrowia Lipolytica

Publications (1)

Publication Number Publication Date
ATE73848T1 true ATE73848T1 (de) 1992-04-15

Family

ID=24151875

Family Applications (1)

Application Number Title Priority Date Filing Date
AT84306734T ATE73848T1 (de) 1983-10-06 1984-10-03 Verfahren zur transformation von yarrowia lipolytica.

Country Status (17)

Country Link
EP (1) EP0138508B1 (de)
JP (2) JP2559689B2 (de)
KR (1) KR870000239B1 (de)
AT (1) ATE73848T1 (de)
AU (1) AU554118B2 (de)
BR (1) BR8404984A (de)
DE (1) DE3485589D1 (de)
DK (2) DK171878B1 (de)
ES (3) ES8605038A1 (de)
FI (1) FI85987C (de)
IE (1) IE57685B1 (de)
IL (1) IL73119A (de)
IN (1) IN163393B (de)
NO (1) NO173743C (de)
NZ (1) NZ209794A (de)
PH (1) PH25363A (de)
ZA (1) ZA847824B (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3572688D1 (en) * 1984-06-26 1989-10-05 Agronomique Inst Nat Rech Transformation vector for yeast yarrowia lipolytica, transformation process and transformed yeast
FR2579617B2 (fr) * 1985-04-02 1987-06-26 Agronomique Inst Nat Rech Vecteurs de transformation de la levure yarrowia lipolytica, procede de transformation et levure transformee
US4937189A (en) * 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
GB8529275D0 (en) * 1985-11-28 1986-01-02 Whitbread & Co Plc Dna recombination
JPH082304B2 (ja) * 1987-12-17 1996-01-17 オリエンタル酵母工業株式会社 プラスミドとその製造方法
FR2626584B1 (fr) * 1988-01-28 1990-07-13 Agronomique Inst Nat Rech Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation
FR2734843A1 (fr) * 1995-06-02 1996-12-06 Centre Nat Rech Scient Nouveau procede de bioconversion microbienne.
EP0747484A1 (de) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Aufwährts-aktivierungssequenzen und rekombinante Promotorsequenzen die in Yarrowia funktionell sind, und Vektoren die sie enthalten
GB9715905D0 (en) * 1997-07-28 1997-10-01 Ciba Geigy Ag Plant phosphoribosyl transferase and DNA coding thereof
FR2782733B1 (fr) * 1998-09-01 2002-02-08 Agronomique Inst Nat Rech Procede de transformation non-homologue de yarrowia lipolytica
EP1492874B1 (de) * 2002-03-29 2011-01-19 XOMA Technology Ltd. Multigene vectoren und methoden für eine erhöhte expression von rekombinanten polypeptiden
WO2006102342A2 (en) 2005-03-18 2006-09-28 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
KR20090058077A (ko) * 2007-12-04 2009-06-09 충북대학교 산학협력단 생물체 정보의 염색체 내 표지 방법 및 그 정보가 표지된생물체
FR2927089B1 (fr) 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
UA108343C2 (uk) * 2008-05-23 2015-04-27 Нуцеліс Інк. Спосіб одержання сквалену за допомогою дріжджів
US20100298612A1 (en) 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols

Also Published As

Publication number Publication date
ZA847824B (en) 1986-05-28
EP0138508A1 (de) 1985-04-24
JP2559689B2 (ja) 1996-12-04
ES8802399A1 (es) 1988-05-16
DK171879B1 (da) 1997-07-28
ES8900255A1 (es) 1989-05-01
ES548940A0 (es) 1988-05-16
DK477084A (da) 1985-05-15
IN163393B (de) 1988-09-17
KR850003167A (ko) 1985-06-13
KR870000239B1 (ko) 1987-02-18
ES548941A0 (es) 1989-05-01
FI843932A0 (fi) 1984-10-05
BR8404984A (pt) 1985-08-20
JPS60199386A (ja) 1985-10-08
AU554118B2 (en) 1986-08-07
IL73119A (en) 1990-01-18
JPH09131176A (ja) 1997-05-20
ES536546A0 (es) 1986-02-16
DK148195A (da) 1995-12-28
IL73119A0 (en) 1984-12-31
FI843932L (fi) 1985-04-07
NZ209794A (en) 1989-01-27
JP2718659B2 (ja) 1998-02-25
AU3387684A (en) 1985-04-18
DK477084D0 (da) 1984-10-05
NO173743B (no) 1993-10-18
DE3485589D1 (de) 1992-04-23
FI85987B (fi) 1992-03-13
ES8605038A1 (es) 1986-02-16
IE57685B1 (en) 1993-02-24
IE842535L (en) 1985-04-06
NO844001L (no) 1985-04-09
PH25363A (en) 1991-05-13
FI85987C (fi) 1992-06-25
NO173743C (no) 1994-01-26
EP0138508B1 (de) 1992-03-18
DK171878B1 (da) 1997-07-28

Similar Documents

Publication Publication Date Title
ATE73848T1 (de) Verfahren zur transformation von yarrowia lipolytica.
DK101385A (da) Molekylaer kloning og ekspression i industrielle mikroorganismearter
IL87579A0 (en) The manufacture and expression of large structural genes
DK139493A (da) Rekombinant plasmid, bakteriekultur transformeret dermed samt kulturens anvendelse
EP0144064A3 (en) Method for producing interferons
ATE199168T1 (de) Stabilisierung von plasmiden
KR880012756A (ko) Dna 및 그의 이용
DE3888386T2 (de) Verfahren zur Herstellung von Proteinen.
DK391185A (da) Rekombinant saccharomyces
ATE107359T1 (de) Thermo-induzierbare expression von heterologen genen in bacillus subtilis und mittel und verfahren zu deren ausführung.
JPS6486875A (en) Plasmid vector containing streptomycin-sensitive gene

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time