ES2817802T3 - Métodos para purificar una proteína objetivo de una o más impurezas en una muestra - Google Patents

Métodos para purificar una proteína objetivo de una o más impurezas en una muestra Download PDF

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Publication number
ES2817802T3
ES2817802T3 ES10807153T ES10807153T ES2817802T3 ES 2817802 T3 ES2817802 T3 ES 2817802T3 ES 10807153 T ES10807153 T ES 10807153T ES 10807153 T ES10807153 T ES 10807153T ES 2817802 T3 ES2817802 T3 ES 2817802T3
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Spain
Prior art keywords
buffer
protein
region
cation exchange
target protein
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ES10807153T
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English (en)
Spanish (es)
Inventor
Neil Soice
John Dana Hubbard
Yu Zhang
James Hamzik
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EMD Millipore Corp
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EMD Millipore Corp
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
ES10807153T 2009-08-07 2010-08-05 Métodos para purificar una proteína objetivo de una o más impurezas en una muestra Active ES2817802T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27370909P 2009-08-07 2009-08-07
PCT/US2010/044539 WO2011017514A1 (en) 2009-08-07 2010-08-05 Methods for purifying a target protein from one or more impurities in a sample

Publications (1)

Publication Number Publication Date
ES2817802T3 true ES2817802T3 (es) 2021-04-08

Family

ID=43544660

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10807153T Active ES2817802T3 (es) 2009-08-07 2010-08-05 Métodos para purificar una proteína objetivo de una o más impurezas en una muestra

Country Status (9)

Country Link
US (1) US8536316B2 (enExample)
EP (1) EP2462157B1 (enExample)
JP (2) JP5791602B2 (enExample)
KR (1) KR101415660B1 (enExample)
CN (2) CN107188960A (enExample)
ES (1) ES2817802T3 (enExample)
IN (1) IN2012DN00338A (enExample)
SG (1) SG177577A1 (enExample)
WO (1) WO2011017514A1 (enExample)

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JPWO2015041218A1 (ja) * 2013-09-17 2017-03-02 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎＭｅｔｈｏｄａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎｍｅｔｈｏｄｕｓｉｎｇＣａｔｉｏｎＥｘｃｈａｎｇｅｒａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）
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WO2015198320A1 (en) * 2014-06-24 2015-12-30 Insight Biopharmaceuticals Ltd. Methods of purifying antibodies
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EP3230299A1 (en) 2014-12-08 2017-10-18 EMD Millipore Corporation Mixed bed ion exchange adsorber
KR102566292B1 (ko) 2015-06-05 2023-08-10 더블유.알. 그레이스 앤드 캄파니-콘. 흡착성 바이오프로세싱 정화제와 이의 제조 및 사용 방법
JP7163187B2 (ja) * 2016-04-05 2022-10-31 ヘモネティクス・コーポレーション 血液からの免疫グロブリンの濃縮のための方法および装置
CN107964044B (zh) * 2016-10-19 2021-11-19 无锡科捷诺生物科技有限责任公司 从乳样中纯化抗cd20单克隆抗体的方法
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
WO2020125757A1 (en) * 2018-12-21 2020-06-25 Wuxi Biologics (Shanghai) Co., Ltd. A method for improving aggregate removal by protein a chromatography
FR3099066B1 (fr) 2019-07-26 2021-08-13 Novasep Process Procédé de purification d’une substance cible avec inactivation virale
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
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CN110724204B (zh) * 2019-11-18 2021-10-22 广东菲鹏制药股份有限公司 Fc融合蛋白的纯化方法
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Also Published As

Publication number Publication date
IN2012DN00338A (enExample) 2015-05-22
JP6335838B2 (ja) 2018-05-30
SG177577A1 (en) 2012-03-29
EP2462157A1 (en) 2012-06-13
KR101415660B1 (ko) 2014-07-16
CN102574911A (zh) 2012-07-11
CN102574911B (zh) 2017-06-06
WO2011017514A1 (en) 2011-02-10
US8536316B2 (en) 2013-09-17
CN107188960A (zh) 2017-09-22
JP5791602B2 (ja) 2015-10-07
JP2015157846A (ja) 2015-09-03
US20110065901A1 (en) 2011-03-17
KR20120089432A (ko) 2012-08-10
EP2462157A4 (en) 2012-12-26
EP2462157B1 (en) 2020-06-17
JP2013501721A (ja) 2013-01-17

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