CN107188960A - 从样品的一或多种杂质中纯化靶蛋白的方法 - Google Patents

从样品的一或多种杂质中纯化靶蛋白的方法 Download PDF

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Publication number
CN107188960A
CN107188960A CN201710418825.9A CN201710418825A CN107188960A CN 107188960 A CN107188960 A CN 107188960A CN 201710418825 A CN201710418825 A CN 201710418825A CN 107188960 A CN107188960 A CN 107188960A
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CN
China
Prior art keywords
protein
buffer
column
cation exchange
resin
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Pending
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CN201710418825.9A
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English (en)
Chinese (zh)
Inventor
N·索伊斯
J·D·哈伯德
Y·张
J·海姆齐克
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EMD Millipore Corp
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Millipore Corp
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Publication of CN107188960A publication Critical patent/CN107188960A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
CN201710418825.9A 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法 Pending CN107188960A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27370909P 2009-08-07 2009-08-07
US61/273,709 2009-08-07
CN201080035153.XA CN102574911B (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201080035153.XA Division CN102574911B (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法

Publications (1)

Publication Number Publication Date
CN107188960A true CN107188960A (zh) 2017-09-22

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ID=43544660

Family Applications (2)

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CN201710418825.9A Pending CN107188960A (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法
CN201080035153.XA Expired - Fee Related CN102574911B (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法

Family Applications After (1)

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CN201080035153.XA Expired - Fee Related CN102574911B (zh) 2009-08-07 2010-08-05 从样品的一或多种杂质中纯化靶蛋白的方法

Country Status (9)

Country Link
US (1) US8536316B2 (enExample)
EP (1) EP2462157B1 (enExample)
JP (2) JP5791602B2 (enExample)
KR (1) KR101415660B1 (enExample)
CN (2) CN107188960A (enExample)
ES (1) ES2817802T3 (enExample)
IN (1) IN2012DN00338A (enExample)
SG (1) SG177577A1 (enExample)
WO (1) WO2011017514A1 (enExample)

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EP3041593B1 (en) * 2013-09-05 2025-01-29 F. Hoffmann-La Roche AG Method of cleaning a chromatography material for its reuse
US10519195B2 (en) * 2013-09-17 2019-12-31 Kaneka Corporation Antibody purification method, antibody obtained therefrom, novel antibody purification method using cation exchanger, and antibody obtained therefrom
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JP6612242B2 (ja) 2014-02-14 2019-11-27 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 自動化された多段階精製システム及び多段階クロマトグラフィー精製方法
HUE048782T2 (hu) 2014-03-10 2020-08-28 Richter Gedeon Nyrt Immunoglobulin tisztítása elõtisztítási lépések alkalmazásával
CN104826617B (zh) * 2014-03-25 2017-06-13 西北大学 一种用于蛋白质分离的wax/hic双功能混合模式色谱固定相及其制备方法
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CN107921407B (zh) 2015-06-05 2022-07-05 格雷斯公司 生物处理吸附澄清剂及其制备和使用方法
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CN107964044B (zh) * 2016-10-19 2021-11-19 无锡科捷诺生物科技有限责任公司 从乳样中纯化抗cd20单克隆抗体的方法
CN119881293A (zh) 2018-03-21 2025-04-25 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
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TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
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Also Published As

Publication number Publication date
WO2011017514A1 (en) 2011-02-10
IN2012DN00338A (enExample) 2015-05-22
EP2462157B1 (en) 2020-06-17
JP6335838B2 (ja) 2018-05-30
KR101415660B1 (ko) 2014-07-16
US8536316B2 (en) 2013-09-17
JP2013501721A (ja) 2013-01-17
JP5791602B2 (ja) 2015-10-07
EP2462157A1 (en) 2012-06-13
KR20120089432A (ko) 2012-08-10
CN102574911A (zh) 2012-07-11
ES2817802T3 (es) 2021-04-08
EP2462157A4 (en) 2012-12-26
CN102574911B (zh) 2017-06-06
JP2015157846A (ja) 2015-09-03
SG177577A1 (en) 2012-03-29
US20110065901A1 (en) 2011-03-17

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Application publication date: 20170922