ES2664194T3 - Método novedoso para la producción de células diferenciadas - Google Patents
Método novedoso para la producción de células diferenciadas Download PDFInfo
- Publication number
- ES2664194T3 ES2664194T3 ES10817186.9T ES10817186T ES2664194T3 ES 2664194 T3 ES2664194 T3 ES 2664194T3 ES 10817186 T ES10817186 T ES 10817186T ES 2664194 T3 ES2664194 T3 ES 2664194T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- myc
- bmi1
- results
- analysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004519 manufacturing process Methods 0.000 title abstract description 3
- 238000000034 method Methods 0.000 title description 2
- 210000004027 cell Anatomy 0.000 abstract description 40
- 101000872170 Homo sapiens Polycomb complex protein BMI-1 Proteins 0.000 abstract description 16
- 230000014509 gene expression Effects 0.000 abstract description 10
- 108090000623 proteins and genes Proteins 0.000 abstract description 10
- 210000003593 megakaryocyte Anatomy 0.000 abstract description 5
- 210000003958 hematopoietic stem cell Anatomy 0.000 abstract description 3
- 230000001939 inductive effect Effects 0.000 abstract description 3
- 210000002361 Megakaryocyte Progenitor Cell Anatomy 0.000 abstract description 2
- 108700020796 Oncogene Proteins 0.000 abstract description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 abstract 1
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 abstract 1
- 108091057508 Myc family Proteins 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 102100033566 Polycomb complex protein BMI-1 Human genes 0.000 description 15
- 238000004458 analytical method Methods 0.000 description 9
- 230000004069 differentiation Effects 0.000 description 7
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 4
- 230000004913 activation Effects 0.000 description 3
- 210000001671 embryonic stem cell Anatomy 0.000 description 3
- 229960005309 estradiol Drugs 0.000 description 3
- 102000006495 integrins Human genes 0.000 description 3
- 108010044426 integrins Proteins 0.000 description 3
- 101150062914 BMI1 gene Proteins 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 101150085741 Hoxa2 gene Proteins 0.000 description 1
- 102100025306 Integrin alpha-IIb Human genes 0.000 description 1
- 102000008607 Integrin beta3 Human genes 0.000 description 1
- 108010020950 Integrin beta3 Proteins 0.000 description 1
- 101100058550 Mus musculus Bmi1 gene Proteins 0.000 description 1
- 108010035030 Platelet Membrane Glycoprotein IIb Proteins 0.000 description 1
- 102100034173 Platelet glycoprotein Ib alpha chain Human genes 0.000 description 1
- 101710107770 Platelet glycoprotein Ib alpha chain Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0641—Erythrocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/18—Erythrocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A61P35/02—Antineoplastic agents specific for leukemia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0644—Platelets; Megakaryocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/48—Regulators of apoptosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Epidemiology (AREA)
- Developmental Biology & Embryology (AREA)
- Diabetes (AREA)
- Virology (AREA)
- Oncology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009213645 | 2009-09-15 | ||
| JP2009213645 | 2009-09-15 | ||
| PCT/JP2010/065903 WO2011034073A1 (ja) | 2009-09-15 | 2010-09-15 | 分化細胞の新規製造法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2664194T3 true ES2664194T3 (es) | 2018-04-18 |
Family
ID=43758674
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES10817186.9T Active ES2664194T3 (es) | 2009-09-15 | 2010-09-15 | Método novedoso para la producción de células diferenciadas |
| ES16202612T Active ES2725688T3 (es) | 2009-09-15 | 2010-09-15 | Método novedoso para la producción de células diferenciadas |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES16202612T Active ES2725688T3 (es) | 2009-09-15 | 2010-09-15 | Método novedoso para la producción de células diferenciadas |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US9200254B2 (enExample) |
| EP (2) | EP3159001B1 (enExample) |
| JP (4) | JP5791191B2 (enExample) |
| KR (2) | KR101834687B1 (enExample) |
| CN (2) | CN102712906B (enExample) |
| AU (1) | AU2010296415B2 (enExample) |
| BR (2) | BR112012005710B1 (enExample) |
| CA (1) | CA2774193C (enExample) |
| DK (2) | DK2500418T3 (enExample) |
| ES (2) | ES2664194T3 (enExample) |
| IN (1) | IN2012DN02642A (enExample) |
| RU (2) | RU2603094C2 (enExample) |
| WO (1) | WO2011034073A1 (enExample) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5861191B2 (ja) * | 2010-09-30 | 2016-02-16 | 国立大学法人 熊本大学 | ミエロイド系血液細胞の製造方法 |
| JP5824760B2 (ja) * | 2011-05-13 | 2015-12-02 | 国立大学法人 東京大学 | 多核化巨核球細胞、及び血小板の製造方法 |
| US20140227780A1 (en) | 2011-10-03 | 2014-08-14 | Nissan Chemical Industries, Ltd. | Method for producing megakaryocytes and/or platelets from pluripotent stem cells |
| GB201210857D0 (en) | 2012-06-19 | 2012-08-01 | Cambridge Entpr Ltd | Transcription factor mediated programming towards megakaryocytes |
| EP3973967A1 (en) | 2012-12-21 | 2022-03-30 | Astellas Institute for Regenerative Medicine | Methods for production of platelets from pluripotent stem cells and compositions thereof |
| JP6495658B2 (ja) * | 2013-02-08 | 2019-04-03 | 国立大学法人京都大学 | 巨核球及び血小板の製造方法 |
| WO2014168255A1 (ja) * | 2013-04-12 | 2014-10-16 | 国立大学法人京都大学 | 巨核球の成熟化促進物質 |
| US20160060601A1 (en) | 2013-04-17 | 2016-03-03 | Nissan Chemical Industries, Ltd. | Medium composition and method for producing red blood cells using same |
| US10260043B2 (en) * | 2013-05-15 | 2019-04-16 | University Of Rochester | Human extensively self-renewing erythroblasts (ESRE) |
| WO2015199039A1 (ja) | 2014-06-23 | 2015-12-30 | 東亞合成株式会社 | 細胞の多核化を誘導するペプチドおよびその利用 |
| US20180044634A1 (en) | 2015-03-09 | 2018-02-15 | Megakaryon Corporation | Method for Producing Culture Containing Megakaryocytes, and Method for Producing Platelets Using Same |
| RU2730034C2 (ru) * | 2015-04-14 | 2020-08-14 | Киото Юниверсити | Способ получения клона стволовых клеток, пригодного для индуцирования дифференцировки в соматические клетки |
| KR20160129609A (ko) | 2015-04-30 | 2016-11-09 | 삼성전자주식회사 | Ｂｒａｆ 저해제를 포함하는 세포 또는 개체의 노화를 감소시키기 위한 조성물 및 그의 용도 |
| ES2833357T3 (es) * | 2015-11-02 | 2021-06-15 | Megakaryon Corp | Procedimiento de preparación de plaquetas con un dispositivo de agitación recíproca |
| JP7350246B2 (ja) | 2016-01-29 | 2023-09-26 | 国立大学法人京都大学 | 血小板産生促進剤のスクリーニング方法 |
| EP3453759B1 (en) | 2016-01-29 | 2023-08-30 | Kyoto University | Platelet production promoter and method of producing platelets using same |
| WO2018164040A1 (ja) | 2017-03-06 | 2018-09-13 | 国立大学法人京都大学 | 血小板の製造方法 |
| FR3068366B1 (fr) * | 2017-06-30 | 2023-11-24 | Francais Du Sang Ets | Procede de production de progeniteurs erythroides |
| EP3650535A4 (en) | 2017-07-07 | 2021-03-17 | Kyoto University | PROCESS AND APPARATUS FOR THE PRODUCTION OF PLATES AND METHOD FOR DETERMINING OPERATING CONDITIONS IN AN APPARATUS FOR THE PRODUCTION OF PLATES |
| WO2019059235A1 (ja) | 2017-09-19 | 2019-03-28 | 株式会社メガカリオン | 精製血小板の製造方法、血小板製剤の製造方法、血液製剤の製造方法、血小板保存液、血小板保存剤および血小板の保存方法 |
| US12091683B2 (en) | 2017-09-19 | 2024-09-17 | Megakaryon Corporation | Method for producing platelets, method for producing platelet product, and method for producing blood product |
| MX2020007071A (es) | 2018-01-05 | 2020-11-11 | Platelet Biogenesis Inc | Composiciones y metodos para producir megacariocitos. |
| WO2020006539A1 (en) | 2018-06-29 | 2020-01-02 | Platelet Biogenesis, Inc. | Compositions for drug delivery and methods of use thereof |
| US20200268839A1 (en) * | 2019-02-25 | 2020-08-27 | National Yang-Ming University | Composition for treating pulmonary fibrosis and emphysema and therapeutic method using the same |
| US20220315895A1 (en) * | 2019-05-01 | 2022-10-06 | Transfusion Health, Llc | Methods of making oligopotent and unipotent precursors |
| EP4074321A4 (en) | 2019-12-12 | 2024-01-03 | National University Corporation Chiba University | Freeze-dried preparation containing megakaryocytes and platelets |
| US20230046238A1 (en) | 2019-12-13 | 2023-02-16 | Megakaryon Corporation | Composition and use thereof |
| JPWO2022092169A1 (enExample) | 2020-10-27 | 2022-05-05 | ||
| JP7716851B2 (ja) * | 2020-12-28 | 2025-08-01 | イビデン株式会社 | 凍結保存液 |
| EP4342982A4 (en) | 2021-06-18 | 2025-05-28 | Megakaryon Corporation | Method for producing multinucleated megakaryocytes with increased platelet production capacity, platelet production method, and blood preparation production method |
| EP4365283A4 (en) | 2021-06-30 | 2025-06-11 | National University Corporation Chiba University | Methods for improving the proliferative properties of common myeloid progenitor cells (CMP) or myelocytic progenitor cells |
| WO2024010317A1 (ko) * | 2022-07-04 | 2024-01-11 | 주식회사 아트블러드 | 유전자 과발현 조합을 이용한 적혈구 분화능이 우수한 불멸화 적혈구전구세포주 확립, 이의 제조방법 및 용도 |
| JP2025534107A (ja) | 2022-10-18 | 2025-10-09 | ソングァン メディカル ファンデーション | 幹細胞から血小板前駆細胞への分化誘導用培地組成物及びそれを用いた分化方法 |
| EP4644539A1 (en) | 2022-12-28 | 2025-11-05 | National University Corporation Chiba University | Method for regulating degree of cell differentiation |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US572333A (en) | 1896-12-01 | Evaporating apparatus | ||
| WO1993000433A1 (en) * | 1991-06-28 | 1993-01-07 | New England Deaconess Hospital | A differentiated megakaryocyte line producing novel megakaryocyte differentiation factors |
| GB9502022D0 (en) * | 1995-02-02 | 1995-03-22 | Abuljadayel Ilham M S | A method for preparing lymphohaematopoietic progenitor cells |
| US5723333A (en) | 1995-02-10 | 1998-03-03 | Regents Of The University Of California | Human pancreatic cell lines: developments and uses |
| AU716854B2 (en) * | 1995-02-10 | 2000-03-09 | Regents Of The University Of California, The | Human pancreatic cell lines: developments and uses |
| US20050159378A1 (en) * | 2001-05-18 | 2005-07-21 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of Myc and/or Myb gene expression using short interfering nucleic acid (siNA) |
| JP4344696B2 (ja) * | 2002-09-27 | 2009-10-14 | バイオイー， インコーポレイテッド | 細胞分離組成物および細胞分離法 |
| JP3896482B2 (ja) * | 2002-10-07 | 2007-03-22 | 独立行政法人産業技術総合研究所 | 分化細胞の作製方法 |
| JP2004350601A (ja) | 2003-05-29 | 2004-12-16 | Tanabe Seiyaku Co Ltd | 霊長類動物の胚性幹細胞から造血系細胞への分化方法 |
| JP4706208B2 (ja) | 2004-08-27 | 2011-06-22 | 株式会社アイル | 造血幹細胞の製造方法 |
| JP2006141356A (ja) | 2004-11-24 | 2006-06-08 | Institute Of Physical & Chemical Research | Ｅｓ細胞から樹立された造血幹細胞 |
| AU2006304392B2 (en) | 2005-10-18 | 2014-05-01 | National Jewish Health | Conditionally immortalized long-term stem cells and methods of making and using such cells |
| WO2007056423A1 (en) * | 2005-11-07 | 2007-05-18 | The General Hospital Corporation | Methods and compositions for modulation of stem cell aging |
| KR101420740B1 (ko) * | 2005-12-13 | 2014-07-17 | 교또 다이가꾸 | 핵초기화 인자 |
| US8058064B2 (en) | 2006-10-04 | 2011-11-15 | The University Of Tokyo | Sac-like structure enclosing hematopoietic progenitor cells produced from ES cells and method for preparing blood cells |
| KR101564044B1 (ko) | 2007-10-31 | 2015-10-28 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 핵초기화 방법 |
| CN101492676B (zh) * | 2008-09-16 | 2011-02-16 | 中国科学院广州生物医药与健康研究院 | 用脑膜细胞生成诱导的多能性干细胞的方法及其用途 |
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2010
- 2010-09-15 ES ES10817186.9T patent/ES2664194T3/es active Active
- 2010-09-15 RU RU2012114427/10A patent/RU2603094C2/ru active
- 2010-09-15 DK DK10817186.9T patent/DK2500418T3/en active
- 2010-09-15 CN CN201080042161.7A patent/CN102712906B/zh active Active
- 2010-09-15 BR BR112012005710A patent/BR112012005710B1/pt active IP Right Grant
- 2010-09-15 AU AU2010296415A patent/AU2010296415B2/en active Active
- 2010-09-15 KR KR1020167027550A patent/KR101834687B1/ko active Active
- 2010-09-15 US US13/496,321 patent/US9200254B2/en active Active
- 2010-09-15 EP EP16202612.4A patent/EP3159001B1/en active Active
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