KR101834687B1 - 분화 세포의 신규 제조법 - Google Patents

분화 세포의 신규 제조법 Download PDF

Info

Publication number
KR101834687B1
KR101834687B1 KR1020167027550A KR20167027550A KR101834687B1 KR 101834687 B1 KR101834687 B1 KR 101834687B1 KR 1020167027550 A KR1020167027550 A KR 1020167027550A KR 20167027550 A KR20167027550 A KR 20167027550A KR 101834687 B1 KR101834687 B1 KR 101834687B1
Authority
KR
South Korea
Prior art keywords
cells
gene
cell
myc
megakaryotic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020167027550A
Other languages
English (en)
Korean (ko)
Other versions
KR20160121592A (ko
Inventor
고지 에토
나오야 다카야마
소 나카무라
히로미츠 나카우치
Original Assignee
고쿠리츠다이가쿠호우진 도쿄다이가쿠
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 고쿠리츠다이가쿠호우진 도쿄다이가쿠 filed Critical 고쿠리츠다이가쿠호우진 도쿄다이가쿠
Publication of KR20160121592A publication Critical patent/KR20160121592A/ko
Application granted granted Critical
Publication of KR101834687B1 publication Critical patent/KR101834687B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • C12N5/0641Erythrocytes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14Blood; Artificial blood
    • A61K35/18Erythrocytes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • A61P35/02Antineoplastic agents specific for leukemia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06Antianaemics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • C12N5/0644Platelets; Megakaryocytes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • C12N5/0647Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/48Regulators of apoptosis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60Transcription factors
    • C12N2501/606Transcription factors c-Myc
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Epidemiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Diabetes (AREA)
  • Virology (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020167027550A 2009-09-15 2010-09-15 분화 세포의 신규 제조법 Active KR101834687B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2009-213645 2009-09-15
JP2009213645 2009-09-15
PCT/JP2010/065903 WO2011034073A1 (ja) 2009-09-15 2010-09-15 分化細胞の新規製造法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020127009225A Division KR101667834B1 (ko) 2009-09-15 2010-09-15 분화 세포의 신규 제조법

Publications (2)

Publication Number Publication Date
KR20160121592A KR20160121592A (ko) 2016-10-19
KR101834687B1 true KR101834687B1 (ko) 2018-03-05

Family

ID=43758674

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020167027550A Active KR101834687B1 (ko) 2009-09-15 2010-09-15 분화 세포의 신규 제조법
KR1020127009225A Active KR101667834B1 (ko) 2009-09-15 2010-09-15 분화 세포의 신규 제조법

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020127009225A Active KR101667834B1 (ko) 2009-09-15 2010-09-15 분화 세포의 신규 제조법

Country Status (13)

Country Link
US (2) US9200254B2 (enExample)
EP (2) EP3159001B1 (enExample)
JP (4) JP5791191B2 (enExample)
KR (2) KR101834687B1 (enExample)
CN (2) CN102712906B (enExample)
AU (1) AU2010296415B2 (enExample)
BR (2) BR112012005710B1 (enExample)
CA (1) CA2774193C (enExample)
DK (2) DK2500418T3 (enExample)
ES (2) ES2664194T3 (enExample)
IN (1) IN2012DN02642A (enExample)
RU (2) RU2603094C2 (enExample)
WO (1) WO2011034073A1 (enExample)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5861191B2 (ja) * 2010-09-30 2016-02-16 国立大学法人 熊本大学 ミエロイド系血液細胞の製造方法
JP5824760B2 (ja) * 2011-05-13 2015-12-02 国立大学法人 東京大学 多核化巨核球細胞、及び血小板の製造方法
US20140227780A1 (en) 2011-10-03 2014-08-14 Nissan Chemical Industries, Ltd. Method for producing megakaryocytes and/or platelets from pluripotent stem cells
GB201210857D0 (en) 2012-06-19 2012-08-01 Cambridge Entpr Ltd Transcription factor mediated programming towards megakaryocytes
EP3973967A1 (en) 2012-12-21 2022-03-30 Astellas Institute for Regenerative Medicine Methods for production of platelets from pluripotent stem cells and compositions thereof
JP6495658B2 (ja) * 2013-02-08 2019-04-03 国立大学法人京都大学 巨核球及び血小板の製造方法
WO2014168255A1 (ja) * 2013-04-12 2014-10-16 国立大学法人京都大学 巨核球の成熟化促進物質
US20160060601A1 (en) 2013-04-17 2016-03-03 Nissan Chemical Industries, Ltd. Medium composition and method for producing red blood cells using same
US10260043B2 (en) * 2013-05-15 2019-04-16 University Of Rochester Human extensively self-renewing erythroblasts (ESRE)
WO2015199039A1 (ja) 2014-06-23 2015-12-30 東亞合成株式会社 細胞の多核化を誘導するペプチドおよびその利用
US20180044634A1 (en) 2015-03-09 2018-02-15 Megakaryon Corporation Method for Producing Culture Containing Megakaryocytes, and Method for Producing Platelets Using Same
RU2730034C2 (ru) * 2015-04-14 2020-08-14 Киото Юниверсити Способ получения клона стволовых клеток, пригодного для индуцирования дифференцировки в соматические клетки
KR20160129609A (ko) 2015-04-30 2016-11-09 삼성전자주식회사 Braf 저해제를 포함하는 세포 또는 개체의 노화를 감소시키기 위한 조성물 및 그의 용도
ES2833357T3 (es) * 2015-11-02 2021-06-15 Megakaryon Corp Procedimiento de preparación de plaquetas con un dispositivo de agitación recíproca
JP7350246B2 (ja) 2016-01-29 2023-09-26 国立大学法人京都大学 血小板産生促進剤のスクリーニング方法
EP3453759B1 (en) 2016-01-29 2023-08-30 Kyoto University Platelet production promoter and method of producing platelets using same
WO2018164040A1 (ja) 2017-03-06 2018-09-13 国立大学法人京都大学 血小板の製造方法
FR3068366B1 (fr) * 2017-06-30 2023-11-24 Francais Du Sang Ets Procede de production de progeniteurs erythroides
EP3650535A4 (en) 2017-07-07 2021-03-17 Kyoto University PROCESS AND APPARATUS FOR THE PRODUCTION OF PLATES AND METHOD FOR DETERMINING OPERATING CONDITIONS IN AN APPARATUS FOR THE PRODUCTION OF PLATES
WO2019059235A1 (ja) 2017-09-19 2019-03-28 株式会社メガカリオン 精製血小板の製造方法、血小板製剤の製造方法、血液製剤の製造方法、血小板保存液、血小板保存剤および血小板の保存方法
US12091683B2 (en) 2017-09-19 2024-09-17 Megakaryon Corporation Method for producing platelets, method for producing platelet product, and method for producing blood product
MX2020007071A (es) 2018-01-05 2020-11-11 Platelet Biogenesis Inc Composiciones y metodos para producir megacariocitos.
WO2020006539A1 (en) 2018-06-29 2020-01-02 Platelet Biogenesis, Inc. Compositions for drug delivery and methods of use thereof
US20200268839A1 (en) * 2019-02-25 2020-08-27 National Yang-Ming University Composition for treating pulmonary fibrosis and emphysema and therapeutic method using the same
US20220315895A1 (en) * 2019-05-01 2022-10-06 Transfusion Health, Llc Methods of making oligopotent and unipotent precursors
EP4074321A4 (en) 2019-12-12 2024-01-03 National University Corporation Chiba University Freeze-dried preparation containing megakaryocytes and platelets
US20230046238A1 (en) 2019-12-13 2023-02-16 Megakaryon Corporation Composition and use thereof
JPWO2022092169A1 (enExample) 2020-10-27 2022-05-05
JP7716851B2 (ja) * 2020-12-28 2025-08-01 イビデン株式会社 凍結保存液
EP4342982A4 (en) 2021-06-18 2025-05-28 Megakaryon Corporation Method for producing multinucleated megakaryocytes with increased platelet production capacity, platelet production method, and blood preparation production method
EP4365283A4 (en) 2021-06-30 2025-06-11 National University Corporation Chiba University Methods for improving the proliferative properties of common myeloid progenitor cells (CMP) or myelocytic progenitor cells
WO2024010317A1 (ko) * 2022-07-04 2024-01-11 주식회사 아트블러드 유전자 과발현 조합을 이용한 적혈구 분화능이 우수한 불멸화 적혈구전구세포주 확립, 이의 제조방법 및 용도
JP2025534107A (ja) 2022-10-18 2025-10-09 ソングァン メディカル ファンデーション 幹細胞から血小板前駆細胞への分化誘導用培地組成物及びそれを用いた分化方法
EP4644539A1 (en) 2022-12-28 2025-11-05 National University Corporation Chiba University Method for regulating degree of cell differentiation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009511081A (ja) * 2005-10-18 2009-03-19 ナショナル ジューイッシュ メディカル アンド リサーチ センター 条件的に不死化された長期幹細胞およびそのような細胞を作製および使用する方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US572333A (en) 1896-12-01 Evaporating apparatus
WO1993000433A1 (en) * 1991-06-28 1993-01-07 New England Deaconess Hospital A differentiated megakaryocyte line producing novel megakaryocyte differentiation factors
GB9502022D0 (en) * 1995-02-02 1995-03-22 Abuljadayel Ilham M S A method for preparing lymphohaematopoietic progenitor cells
US5723333A (en) 1995-02-10 1998-03-03 Regents Of The University Of California Human pancreatic cell lines: developments and uses
AU716854B2 (en) * 1995-02-10 2000-03-09 Regents Of The University Of California, The Human pancreatic cell lines: developments and uses
US20050159378A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Myc and/or Myb gene expression using short interfering nucleic acid (siNA)
JP4344696B2 (ja) * 2002-09-27 2009-10-14 バイオイー, インコーポレイテッド 細胞分離組成物および細胞分離法
JP3896482B2 (ja) * 2002-10-07 2007-03-22 独立行政法人産業技術総合研究所 分化細胞の作製方法
JP2004350601A (ja) 2003-05-29 2004-12-16 Tanabe Seiyaku Co Ltd 霊長類動物の胚性幹細胞から造血系細胞への分化方法
JP4706208B2 (ja) 2004-08-27 2011-06-22 株式会社アイル 造血幹細胞の製造方法
JP2006141356A (ja) 2004-11-24 2006-06-08 Institute Of Physical & Chemical Research Es細胞から樹立された造血幹細胞
WO2007056423A1 (en) * 2005-11-07 2007-05-18 The General Hospital Corporation Methods and compositions for modulation of stem cell aging
KR101420740B1 (ko) * 2005-12-13 2014-07-17 교또 다이가꾸 핵초기화 인자
US8058064B2 (en) 2006-10-04 2011-11-15 The University Of Tokyo Sac-like structure enclosing hematopoietic progenitor cells produced from ES cells and method for preparing blood cells
KR101564044B1 (ko) 2007-10-31 2015-10-28 고쿠리츠 다이가쿠 호진 교토 다이가쿠 핵초기화 방법
CN101492676B (zh) * 2008-09-16 2011-02-16 中国科学院广州生物医药与健康研究院 用脑膜细胞生成诱导的多能性干细胞的方法及其用途

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009511081A (ja) * 2005-10-18 2009-03-19 ナショナル ジューイッシュ メディカル アンド リサーチ センター 条件的に不死化された長期幹細胞およびそのような細胞を作製および使用する方法

Also Published As

Publication number Publication date
JP2017046719A (ja) 2017-03-09
CN102712906A (zh) 2012-10-03
EP2500418A1 (en) 2012-09-19
BR112012005710A2 (pt) 2017-11-21
US10087419B2 (en) 2018-10-02
CA2774193A1 (en) 2011-03-24
KR20160121592A (ko) 2016-10-19
RU2012114427A (ru) 2013-10-27
JP2015130866A (ja) 2015-07-23
DK3159001T3 (da) 2019-06-17
KR20120064109A (ko) 2012-06-18
JP2015213520A (ja) 2015-12-03
CN102712906B (zh) 2015-09-30
AU2010296415B2 (en) 2014-07-03
BR122013008030B8 (pt) 2021-07-27
BR112012005710B1 (pt) 2020-06-09
EP3159001A1 (en) 2017-04-26
KR101667834B1 (ko) 2016-10-20
US20160115449A1 (en) 2016-04-28
CN105385659A (zh) 2016-03-09
EP2500418A4 (en) 2013-04-10
JPWO2011034073A1 (ja) 2013-02-14
ES2664194T3 (es) 2018-04-18
EP3159001B1 (en) 2019-03-06
DK2500418T3 (en) 2018-04-30
CA2774193C (en) 2020-08-25
CN105385659B (zh) 2019-06-28
RU2661107C1 (ru) 2018-07-11
IN2012DN02642A (enExample) 2015-09-11
AU2010296415A1 (en) 2012-04-05
WO2011034073A1 (ja) 2011-03-24
BR122013008030A2 (pt) 2019-08-06
US9200254B2 (en) 2015-12-01
JP5791191B2 (ja) 2015-10-07
BR122013008030B1 (pt) 2021-01-05
EP2500418B1 (en) 2018-01-17
JP6382917B2 (ja) 2018-08-29
RU2603094C2 (ru) 2016-11-20
US20120238023A1 (en) 2012-09-20
JP6265437B2 (ja) 2018-01-24
ES2725688T3 (es) 2019-09-26

Similar Documents

Publication Publication Date Title
KR101834687B1 (ko) 분화 세포의 신규 제조법
JP5824760B2 (ja) 多核化巨核球細胞、及び血小板の製造方法
AU2017202019B2 (en) Novel Method for Producing Differentiated Cells
HK1233181A (en) Novel method for producing differentiated cells
HK1233181A1 (en) Novel method for producing differentiated cells

Legal Events

Date Code Title Description
A107 Divisional application of patent
A201 Request for examination
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20161005

Application number text: 1020127009225

Filing date: 20120410

PA0201 Request for examination
PG1501 Laying open of application
E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20161101

Patent event code: PE09021S01D

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20170526

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20171221

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20180226

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20180226

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20210219

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20220218

Start annual number: 5

End annual number: 5