ES2554805T3 - Mutante de síntesis de xilitol de Zymomonas que utiliza xilosa para la producción de etanol - Google Patents
Mutante de síntesis de xilitol de Zymomonas que utiliza xilosa para la producción de etanol Download PDFInfo
- Publication number
- ES2554805T3 ES2554805T3 ES07874067.7T ES07874067T ES2554805T3 ES 2554805 T3 ES2554805 T3 ES 2554805T3 ES 07874067 T ES07874067 T ES 07874067T ES 2554805 T3 ES2554805 T3 ES 2554805T3
- Authority
- ES
- Spain
- Prior art keywords
- xylose
- glucose
- gene
- fermentation
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title abstract description 76
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 title abstract description 38
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 title abstract description 24
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 title abstract description 24
- 241000588901 Zymomonas Species 0.000 title abstract description 4
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 title description 19
- 238000004519 manufacturing process Methods 0.000 title description 18
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 title description 13
- 239000000811 xylitol Substances 0.000 title description 13
- 235000010447 xylitol Nutrition 0.000 title description 13
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 title description 13
- 229960002675 xylitol Drugs 0.000 title description 13
- 230000015572 biosynthetic process Effects 0.000 title description 4
- 238000003786 synthesis reaction Methods 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract description 17
- 108010009512 Glucose-fructose oxidoreductase Proteins 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- PJVXUVWGSCCGHT-ZPYZYFCMSA-N (2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;(3s,4r,5r)-1,3,4,5,6-pentahydroxyhexan-2-one Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O.OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)CO PJVXUVWGSCCGHT-ZPYZYFCMSA-N 0.000 abstract description 2
- 108010043652 Transketolase Proteins 0.000 abstract description 2
- 108700040099 Xylose isomerases Proteins 0.000 abstract description 2
- 102100029089 Xylulose kinase Human genes 0.000 abstract description 2
- 235000019534 high fructose corn syrup Nutrition 0.000 abstract description 2
- 230000002829 reductive effect Effects 0.000 abstract description 2
- 108091022915 xylulokinase Proteins 0.000 abstract description 2
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 108020004530 Transaldolase Proteins 0.000 abstract 1
- 102100028601 Transaldolase Human genes 0.000 abstract 1
- 102000014701 Transketolase Human genes 0.000 abstract 1
- 230000000692 anti-sense effect Effects 0.000 abstract 1
- 238000012239 gene modification Methods 0.000 abstract 1
- 230000005017 genetic modification Effects 0.000 abstract 1
- 235000013617 genetically modified food Nutrition 0.000 abstract 1
- 230000002452 interceptive effect Effects 0.000 abstract 1
- 230000032361 posttranscriptional gene silencing Effects 0.000 abstract 1
- 230000001629 suppression Effects 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 38
- 239000012634 fragment Substances 0.000 description 30
- 238000000855 fermentation Methods 0.000 description 29
- 230000004151 fermentation Effects 0.000 description 29
- 239000013612 plasmid Substances 0.000 description 27
- 239000002609 medium Substances 0.000 description 25
- 210000004027 cell Anatomy 0.000 description 24
- 238000010276 construction Methods 0.000 description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 15
- 239000008103 glucose Substances 0.000 description 15
- 238000000034 method Methods 0.000 description 14
- 229960000268 spectinomycin Drugs 0.000 description 14
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 14
- 108091034117 Oligonucleotide Proteins 0.000 description 12
- 239000004098 Tetracycline Substances 0.000 description 12
- 241000588902 Zymomonas mobilis Species 0.000 description 12
- 230000012010 growth Effects 0.000 description 12
- 229960002180 tetracycline Drugs 0.000 description 12
- 229930101283 tetracycline Natural products 0.000 description 12
- 235000019364 tetracycline Nutrition 0.000 description 12
- 150000003522 tetracyclines Chemical class 0.000 description 12
- 239000002773 nucleotide Substances 0.000 description 10
- 125000003729 nucleotide group Chemical group 0.000 description 10
- 206010010144 Completed suicide Diseases 0.000 description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 8
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- 229940041514 candida albicans extract Drugs 0.000 description 7
- 229910052799 carbon Inorganic materials 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 235000000346 sugar Nutrition 0.000 description 7
- 239000012138 yeast extract Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 108010001539 D-lactate dehydrogenase Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 239000007836 KH2PO4 Substances 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 5
- 230000029087 digestion Effects 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 description 5
- 239000012071 phase Substances 0.000 description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- 238000000527 sonication Methods 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 4
- 108020004705 Codon Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 230000005526 G1 to G0 transition Effects 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 239000006227 byproduct Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000004520 electroporation Methods 0.000 description 4
- 101150104734 ldh gene Proteins 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 4
- 235000019341 magnesium sulphate Nutrition 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- 238000009331 sowing Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 150000003741 xylose derivatives Chemical class 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 101100342790 Bacillus anthracis ldh3 gene Proteins 0.000 description 3
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 3
- 102100023319 Dihydrolipoyl dehydrogenase, mitochondrial Human genes 0.000 description 3
- 241001302160 Escherichia coli str. K-12 substr. DH10B Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 101150050566 LDHC gene Proteins 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 101150049837 PGM gene Proteins 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 229960005091 chloramphenicol Drugs 0.000 description 3
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000000600 sorbitol Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 102100033055 Transketolase Human genes 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000006801 homologous recombination Effects 0.000 description 2
- 238000002744 homologous recombination Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- WOLQREOUPKZMEX-BZSNNMDCSA-N (2s)-2-[[(4s)-4-[[(4s)-4-[[4-[(2-amino-4-oxo-1h-pteridin-6-yl)methylamino]benzoyl]amino]-4-carboxybutanoyl]amino]-4-carboxybutanoyl]amino]pentanedioic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(=O)N[C@@H](CCC(=O)N[C@@H](CCC(O)=O)C(O)=O)C(O)=O)C(O)=O)C=C1 WOLQREOUPKZMEX-BZSNNMDCSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 108010029692 Bisphosphoglycerate mutase Proteins 0.000 description 1
- 108010051219 Cre recombinase Proteins 0.000 description 1
- 230000010558 Gene Alterations Effects 0.000 description 1
- 102100024319 Intestinal-type alkaline phosphatase Human genes 0.000 description 1
- 101710184243 Intestinal-type alkaline phosphatase Proteins 0.000 description 1
- 108010088350 Lactate Dehydrogenase 5 Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000011025 Phosphoglycerate Mutase Human genes 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 101150067314 aadA gene Proteins 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009640 blood culture Methods 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000003518 caustics Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000007824 enzymatic assay Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US84781306P | 2006-09-28 | 2006-09-28 | |
| US847813P | 2006-09-28 | ||
| PCT/US2007/020950 WO2008133638A2 (en) | 2006-09-28 | 2007-09-28 | Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2554805T3 true ES2554805T3 (es) | 2015-12-23 |
Family
ID=39926224
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07874067.7T Active ES2554805T3 (es) | 2006-09-28 | 2007-09-28 | Mutante de síntesis de xilitol de Zymomonas que utiliza xilosa para la producción de etanol |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7741119B2 (enExample) |
| EP (1) | EP2066784B1 (enExample) |
| JP (1) | JP5346808B2 (enExample) |
| CN (1) | CN101861385B (enExample) |
| BR (1) | BRPI0715282A2 (enExample) |
| CA (1) | CA2661512C (enExample) |
| ES (1) | ES2554805T3 (enExample) |
| HU (1) | HUE026720T2 (enExample) |
| PL (1) | PL2066784T3 (enExample) |
| WO (1) | WO2008133638A2 (enExample) |
Families Citing this family (90)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7781191B2 (en) * | 2005-04-12 | 2010-08-24 | E. I. Du Pont De Nemours And Company | Treatment of biomass to obtain a target chemical |
| EP2060632A1 (en) * | 2007-10-29 | 2009-05-20 | Technische Universität Berlin | Method of modifying a yeast cell for the production of ethanol |
| BRPI0817151A8 (pt) * | 2007-10-30 | 2018-12-11 | Du Pont | micro-organismo recombinante e processo para gerar o micro-organismo |
| ES2627671T3 (es) * | 2008-03-27 | 2017-07-31 | E. I. Du Pont De Nemours And Company | Promotores de Zymomonas de alta expresión |
| CA2716585C (en) * | 2008-03-27 | 2017-05-09 | E.I. Du Pont De Nemours And Company | Zymomonas recombinant for substituted glyceraldehyde-3-phosphate dehydrogenase promoter and xylose isomerase gene for improved xylose utilization |
| US20100159521A1 (en) * | 2008-12-19 | 2010-06-24 | E. I. Du Pont De Nemours And Company | Ozone treatment of biomass to enhance enzymatic saccharification |
| US8247208B2 (en) | 2008-12-22 | 2012-08-21 | Alliance For Sustainable Energy Llc | Zymomonas with improved xylose utilization in stress conditions |
| US20110143408A1 (en) * | 2009-06-18 | 2011-06-16 | E. I. Du Pont De Nemours And Company | Zymomonas with improved arabinose utilization |
| WO2010151574A2 (en) | 2009-06-25 | 2010-12-29 | Shell Oil Company | Water injection systems and methods |
| WO2011046818A2 (en) | 2009-10-12 | 2011-04-21 | E. I. Du Pont De Nemours And Company | Ammonia pretreatment of biomass for improved inhibitor profile |
| US8647850B2 (en) | 2009-12-23 | 2014-02-11 | E I Du Pont De Nemours And Company | Process for simultaneous saccharification and fermentation for production of ethanol |
| CN107287250A (zh) | 2009-12-23 | 2017-10-24 | 丹尼斯科美国公司 | 提高同步糖化发酵反应效率的方法 |
| US8721794B2 (en) | 2010-04-28 | 2014-05-13 | E I Du Pont De Nemours And Company | Production of high solids syrup from lignocellulosic biomass hydrolysate fermentation broth |
| BR122021005297B1 (pt) | 2010-06-02 | 2022-02-22 | Evolva, Inc | Método para transferir uma segunda porção de açúcar para uma posição c-2' de uma glicose em um glicosídeo de esteviol |
| AU2011268220B2 (en) | 2010-06-18 | 2015-01-29 | Butamax(Tm) Advanced Biofuels Llc | Extraction solvents derived from oil for alcohol removal in extractive fermentation |
| CN102947456A (zh) | 2010-06-18 | 2013-02-27 | 布特马斯先进生物燃料有限责任公司 | 补充脂肪酸以改善醇产量 |
| US9040263B2 (en) | 2010-07-28 | 2015-05-26 | Butamax Advanced Biofuels Llc | Production of alcohol esters and in situ product removal during alcohol fermentation |
| US8623623B2 (en) | 2010-06-29 | 2014-01-07 | E I Du Pont De Nemours And Company | Xylose utilization in recombinant Zymomonas |
| US8679822B2 (en) | 2010-06-29 | 2014-03-25 | E I Du Pont De Nemours And Company | Xylose utilization in recombinant zymomonas |
| WO2012047832A2 (en) | 2010-10-07 | 2012-04-12 | Shell Oil Company | Process for the production of alcohols from biomass |
| CN103189526B (zh) | 2010-11-05 | 2015-07-08 | 国际壳牌研究有限公司 | 处理生物质以产生可用于生物燃料的材料 |
| US8476048B2 (en) | 2010-12-17 | 2013-07-02 | E I Du Pont De Nemours And Company | Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium |
| WO2012088108A1 (en) | 2010-12-20 | 2012-06-28 | Shell Oil Company | Process for the production of alcohols from biomass |
| US20120196342A1 (en) | 2011-02-01 | 2012-08-02 | Rachel Ruizhen Chen | Industrial Applications of A Novel Aldo/Keto Reductase Of Zymomonas Mobilis |
| US20130040340A1 (en) | 2011-02-07 | 2013-02-14 | E. I. Du Pont De Nemours And Company | Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms |
| US8759044B2 (en) | 2011-03-23 | 2014-06-24 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
| US8765425B2 (en) | 2011-03-23 | 2014-07-01 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
| US8765426B2 (en) | 2011-04-07 | 2014-07-01 | E I Du Pont De Nemours And Company | Pantothenic acid biosynthesis in zymomonas |
| MX2013014327A (es) | 2011-06-17 | 2014-01-23 | Butamax Tm Advanced Biofuels | Coproductos de procesos de produccion de biocombustible y metodos para elaborarlos. |
| JP6262653B2 (ja) | 2011-08-08 | 2018-01-17 | エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． | ステビオール配糖体の組換え生産 |
| WO2013086222A2 (en) | 2011-12-09 | 2013-06-13 | Butamax(Tm) Advanced Biofuels Llc | Process to remove product alcohols from fermentation broth |
| US20130157332A1 (en) | 2011-12-20 | 2013-06-20 | E I Du Pont De Nemours And Company | Gene inactivation allowing immediate growth on xylose medium by engineered zymomonas |
| US8911983B2 (en) | 2011-12-20 | 2014-12-16 | E I Du Pont De Nemours And Company | Pnp gene modification for improved xylose utilization in Zymomonas |
| JP2015517303A (ja) | 2012-05-04 | 2015-06-22 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | アルコールの製造および回収のための方法およびシステム |
| CN104395478B (zh) | 2012-05-07 | 2017-07-28 | 国际壳牌研究有限公司 | 处理生物质以生产可用于生物燃料的材料的连续或半连续方法 |
| US8759069B2 (en) | 2012-06-15 | 2014-06-24 | E I Du Pont De Nemours And Company | Contaminant control in Zymomonas fermentation using hop acids |
| US8697426B2 (en) | 2012-06-15 | 2014-04-15 | E I Du Pont De Nemours And Company | Contaminant control in Zymomonas fermentation using virginiamycin |
| US20140024064A1 (en) | 2012-07-23 | 2014-01-23 | Butamax(Tm) Advanced Biofuels Llc | Processes and systems for the production of fermentative alcohols |
| EP2895612A1 (en) | 2012-09-12 | 2015-07-22 | Butamax Advanced Biofuels LLC | Processes and systems for the production of fermentation products |
| US9249076B2 (en) | 2012-09-21 | 2016-02-02 | Butamax Advanced Biofuels Llc | Recyclable extractant compositions |
| AU2013329024A1 (en) | 2012-10-11 | 2015-03-12 | Butamax Advanced Biofuels Llc | Processes and systems for the production of fermentation products |
| BR112015009201A2 (pt) | 2012-10-31 | 2017-11-14 | Danisco Us Inc | composição compreendeendo um polipeptídeo recombinante, ácido nucleico codificando o mesmo, célula hospedeira, bem como métodos para produzir uma beta-glucosidase e para hidrolisar um substrato de biomassa lignocelulósica |
| US20150252343A1 (en) | 2012-10-31 | 2015-09-10 | Danisco Us Inc. | Beta-glucosidase from magnaporthe grisea |
| JP2015533294A (ja) | 2012-10-31 | 2015-11-24 | ダニスコ・ユーエス・インク | ニューロスポラ・クラッサ（Ｎｅｕｒｏｓｐｏｒａｃｒａｓｓａ）由来のβ−グルコシダーゼ |
| WO2014081848A1 (en) | 2012-11-20 | 2014-05-30 | Butamax Advanced Biofuels Llc | Butanol purification |
| US8846357B2 (en) | 2012-12-20 | 2014-09-30 | E I Du Pont De Nemours And Company | Stabilized chlorine dioxide for contamination control in Zymomonas fermentation |
| KR20150115002A (ko) | 2013-02-06 | 2015-10-13 | 에볼바 에스아 | 레바우디오시드 ｄ 및 레바우디오시드 ｍ의 개선된 생산 방법 |
| CN105189771B (zh) | 2013-02-11 | 2019-11-05 | 埃沃尔瓦公司 | 在重组宿主中有效产生甜菊醇糖苷 |
| WO2014130352A1 (en) | 2013-02-21 | 2014-08-28 | Butamax Advanced Biofuels Llc | Recovery of thermal energy as heat source in the production of butanol from a fermentation process |
| US20140273105A1 (en) | 2013-03-12 | 2014-09-18 | E I Du Pont De Nemours And Company | Gradient pretreatment of lignocellulosic biomass |
| WO2014159309A1 (en) | 2013-03-12 | 2014-10-02 | Butamax Advanced Biofuels Llc | Processes and systems for the production of alcohols |
| WO2014160050A1 (en) | 2013-03-14 | 2014-10-02 | Butamax Advanced Biofuels Llc | Glycerol 3- phosphate dehydrogenase for butanol production |
| WO2014144643A1 (en) | 2013-03-15 | 2014-09-18 | Butamax Advanced Biofuels Llc | Method for producing butanol using extractive fermentation |
| WO2014151645A1 (en) | 2013-03-15 | 2014-09-25 | Butamax Advanced Biofuels Llc | Process for maximizing biomass growth and butanol yield by feedback control |
| CA2901374A1 (en) | 2013-03-15 | 2014-09-18 | Butamax Advanced Biofuels Llc | Method for production of butanol using extractive fermentation |
| WO2015002913A1 (en) | 2013-07-03 | 2015-01-08 | Butamax Advanced Biofuels Llc | Partial adaptation for butanol production |
| US9139818B2 (en) * | 2013-09-26 | 2015-09-22 | E I Du Pont De Nemours And Company | High expression Zymomonas promoters |
| US20150087041A1 (en) | 2013-09-26 | 2015-03-26 | E I Du Pont De Nemours And Company | Production of ethanol with reduced contaminants in a cellulosic biomass based process |
| US20150087040A1 (en) | 2013-09-26 | 2015-03-26 | E I Du Pont De Nemours And Company | Production of ethanol and recycle water in a cellulosic fermentation process |
| US9499451B2 (en) | 2013-10-10 | 2016-11-22 | E I Du Pont De Nemours And Company | Soil conditioner compositions containing lignocellulosic biomass fermentation process syrup |
| US20150104842A1 (en) | 2013-10-10 | 2015-04-16 | E I Du Pont De Nemours And Company | Fuel compositions containing lignocellulosic biomass fermentation process syrup |
| US9725363B2 (en) | 2013-10-10 | 2017-08-08 | E I Du Pont De Nemours And Company | Lignocellulosic biomass fermentation process co-product fuel for cement kiln |
| US10227623B2 (en) | 2013-11-24 | 2019-03-12 | E I Du Pont De Nemours And Company | High force and high stress destructuring of cellulosic biomass |
| BR112016012627A2 (pt) | 2013-12-04 | 2017-09-26 | Danisco Us Inc | composições compreendendo um polipeptídeo de beta-glicosidase e métodos de uso |
| WO2015160887A1 (en) | 2014-04-17 | 2015-10-22 | Shell Oil Company | Processes for producing fermentation products |
| US9371546B2 (en) | 2014-06-24 | 2016-06-21 | E I Du Pont De Nemours And Company | Enhancing D-xylose and L-arabinose utilization in Zymomonas cells |
| CA2957331A1 (en) | 2014-08-11 | 2016-02-18 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CN106536496A (zh) | 2014-08-14 | 2017-03-22 | 国际壳牌研究有限公司 | 从生物质制备糠醛的方法 |
| BR112017002204B1 (pt) | 2014-08-14 | 2021-01-12 | Shell Internationale Research Maatschappij B.V. | métodos para recuperar ácido a-hidroxisulfônico em um processo de tratamento de biomassa |
| WO2016025678A1 (en) | 2014-08-14 | 2016-02-18 | Shell Oil Company | Process for preparing furfural from biomass |
| US10612064B2 (en) | 2014-09-09 | 2020-04-07 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| US20170349922A1 (en) | 2014-10-27 | 2017-12-07 | Danisco Us Inc. | Compositions and methods related to beta-glucosidase |
| EP4148137A1 (en) | 2015-01-30 | 2023-03-15 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| CA2979931A1 (en) | 2015-03-16 | 2016-09-22 | Dsm Ip Assets B.V. | Udp-glycosyltransferases |
| BR112018002564A2 (pt) | 2015-08-07 | 2018-09-25 | Evolva Sa | produção de glicosídeos de esteviol em hospedeiros recombinantes |
| WO2017116840A1 (en) | 2015-12-28 | 2017-07-06 | Danisco Us Inc. | Methods for the production of fermentation products from feedstock |
| CN109195457A (zh) | 2016-04-13 | 2019-01-11 | 埃沃尔瓦公司 | 在重组宿主中产生甜菊醇糖苷 |
| US10479773B2 (en) | 2016-05-03 | 2019-11-19 | Shell Oil Company | Lignin-based solvents and methods for their preparation |
| US10815514B2 (en) | 2016-05-16 | 2020-10-27 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CA3039603A1 (en) | 2016-11-01 | 2018-05-11 | Shell Internationale Research Maatschappij B.V. | Process for the recovery of furfural |
| WO2018085182A1 (en) | 2016-11-01 | 2018-05-11 | Shell Oil Company | Process for the recovery of furfural |
| EP3535248A1 (en) | 2016-11-01 | 2019-09-11 | Shell Internationale Research Maatschappij B.V. | Process for the recovery of furfural |
| US10899725B2 (en) | 2016-11-01 | 2021-01-26 | Shell Oil Company | Process for the recovery of furfural |
| CN110100006A (zh) | 2016-11-07 | 2019-08-06 | 埃沃尔瓦公司 | 重组宿主中甜菊糖苷的生产 |
| WO2018106656A1 (en) | 2016-12-06 | 2018-06-14 | Danisco Us Inc | Truncated lpmo enzymes and use thereof |
| WO2019074828A1 (en) | 2017-10-09 | 2019-04-18 | Danisco Us Inc | CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE |
| US11787773B2 (en) | 2019-05-22 | 2023-10-17 | Shell Usa, Inc. | Process for the production of furfural |
| US11649470B2 (en) | 2019-07-19 | 2023-05-16 | ExxonMobil Technology and Engineering Company | Feed control in conversion of biomass into hydrocarbon fuels and chemicals |
| CN113444763B (zh) * | 2021-08-16 | 2022-11-08 | 农业部沼气科学研究所 | 树干毕赤酵母与运动发酵单胞菌混菌发酵葡萄糖和木糖产乙醇的方法 |
| AU2024212235A1 (en) | 2023-01-23 | 2025-07-03 | Shell Internationale Research Maatschappij B.V. | Method for treating grains to produce material useful for chemicals and biofuels |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5231020A (en) * | 1989-03-30 | 1993-07-27 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
| US5514583A (en) * | 1994-04-15 | 1996-05-07 | Midwest Research Institute | Recombinant zymomonas for pentose fermentation |
| US5843760A (en) * | 1994-04-15 | 1998-12-01 | Midwest Research Institute | Single zymomonas mobilis strain for xylose and arabinose fermentation |
| US5712133A (en) * | 1994-04-15 | 1998-01-27 | Midwest Research Institute | Pentose fermentation by recombinant zymomonas |
| WO1995028476A1 (en) | 1994-04-15 | 1995-10-26 | Midwest Research Institute | Recombinant zymomonas for pentose fermentation |
| JP4510355B2 (ja) * | 2000-05-01 | 2010-07-21 | ミッドウエスト リサーチ インスティチュート | 安定なチモモナスモビリスのキシロースおよびアラビノース醗酵株 |
| US7223575B2 (en) * | 2000-05-01 | 2007-05-29 | Midwest Research Institute | Zymomonas pentose-sugar fermenting strains and uses thereof |
| US7374939B1 (en) | 2000-05-01 | 2008-05-20 | Midwest Research Institute | Method of inactivation of an end product of energy metabolism in Zymomonas mobilis |
| US7354755B2 (en) * | 2000-05-01 | 2008-04-08 | Midwest Research Institute | Stable zymomonas mobilis xylose and arabinose fermenting strains |
| US6566107B1 (en) * | 2000-11-01 | 2003-05-20 | Midwest Research Institute | Recombinant Zymomonas mobilis with improved xylose utilization |
| US20040231060A1 (en) | 2003-03-07 | 2004-11-25 | Athenix Corporation | Methods to enhance the activity of lignocellulose-degrading enzymes |
| CN1600850A (zh) | 2003-09-23 | 2005-03-30 | 中国农业科学院生物技术研究所 | 运动发酵单胞菌工程菌及其应用 |
-
2007
- 2007-09-27 US US11/862,566 patent/US7741119B2/en not_active Expired - Fee Related
- 2007-09-28 BR BRPI0715282-5A patent/BRPI0715282A2/pt active Search and Examination
- 2007-09-28 CA CA2661512A patent/CA2661512C/en not_active Expired - Fee Related
- 2007-09-28 JP JP2009530433A patent/JP5346808B2/ja not_active Expired - Fee Related
- 2007-09-28 EP EP07874067.7A patent/EP2066784B1/en not_active Not-in-force
- 2007-09-28 CN CN200780036026XA patent/CN101861385B/zh not_active Expired - Fee Related
- 2007-09-28 PL PL07874067T patent/PL2066784T3/pl unknown
- 2007-09-28 WO PCT/US2007/020950 patent/WO2008133638A2/en not_active Ceased
- 2007-09-28 HU HUE07874067A patent/HUE026720T2/en unknown
- 2007-09-28 ES ES07874067.7T patent/ES2554805T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2066784B1 (en) | 2015-09-16 |
| US20080286870A1 (en) | 2008-11-20 |
| PL2066784T3 (pl) | 2016-03-31 |
| HUE026720T2 (en) | 2016-07-28 |
| JP2010504758A (ja) | 2010-02-18 |
| US7741119B2 (en) | 2010-06-22 |
| WO2008133638A3 (en) | 2009-01-29 |
| CN101861385A (zh) | 2010-10-13 |
| CA2661512A1 (en) | 2008-11-06 |
| CA2661512C (en) | 2014-02-11 |
| JP5346808B2 (ja) | 2013-11-20 |
| CN101861385B (zh) | 2013-02-20 |
| BRPI0715282A2 (pt) | 2013-07-16 |
| WO2008133638A2 (en) | 2008-11-06 |
| WO2008133638A9 (en) | 2008-12-31 |
| EP2066784A2 (en) | 2009-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2554805T3 (es) | Mutante de síntesis de xilitol de Zymomonas que utiliza xilosa para la producción de etanol | |
| ES2385857T3 (es) | Zymomonas con producción aumentada de etanol en medio que contiene azúcares concentrados y acetato | |
| ES2361385T3 (es) | Proceso para la producción de etanol en un medio que comprende xilosa que emplea una cepa de zymomonas recombinante con expresión de hima reducida. | |
| AU2009228323B2 (en) | Zymomonas with improved xylose utilization | |
| ES2599579T3 (es) | Zymomonas con utilización de arabinosa mejorada | |
| JP5185940B2 (ja) | キシロース資化性ザイモモナス（Ｚｙｍｏｍｏｎａｓ）のキシリトール合成変異体を使用するエタノール産生 | |
| ES2546669T3 (es) | Utilización mejorada de xilosa en bacterias Zymomonas recombinantes que presentan una actividad ribosa-5-fosfato aumentada | |
| JP5444323B2 (ja) | 高発現ザイモモナス（Ｚｙｍｏｍｏｎａｓ）プロモーター | |
| ES2396151T3 (es) | Potenciamiento de la producción de etanol microbiana | |
| BRPI0618074A2 (pt) | organismos termofìlicos para conversão de biomassa lignocelulósica em etanol | |
| CA2803878A1 (en) | Improved xylose utilization in recombinant zymomonas having additional xylose isomerase activity | |
| CN117904020A (zh) | 大肠杆菌乙酰化突变菌株的构建及其生产3-羟基丙酸的应用 | |
| US9139818B2 (en) | High expression Zymomonas promoters | |
| CN117802027B (zh) | 盐单胞菌j9u及其衍生菌的构建方法、pha制备方法与应用 | |
| CN116496965A (zh) | 产乙醇的运动发酵单胞菌及应用 | |
| CN103370410A (zh) | 具有增强的糖类利用的突变微生物体及其制备方法 | |
| CN114015634B (zh) | 高产琥珀酸的重组大肠杆菌及其构建方法和应用 | |
| CN101993849A (zh) | 一种解除铵阻遏的生物产氢基因工程菌及其构建方法 | |
| CN116200360A (zh) | 一种futCB突变体及生物合成2’-岩藻糖基乳糖的方法 |