ES2533793T3 - Procedimientos de detección de diana con cebador HDD - Google Patents
Procedimientos de detección de diana con cebador HDD Download PDFInfo
- Publication number
- ES2533793T3 ES2533793T3 ES09851131.4T ES09851131T ES2533793T3 ES 2533793 T3 ES2533793 T3 ES 2533793T3 ES 09851131 T ES09851131 T ES 09851131T ES 2533793 T3 ES2533793 T3 ES 2533793T3
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- primer
- nucleic acid
- hdd
- acid sequence
- target nucleic
- Prior art date
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- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/101—Taqman
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- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
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- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
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Applications Claiming Priority (3)
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| KR20090107262 | 2009-11-07 | ||
| KR1020090107262A KR20110050327A (ko) | 2009-11-07 | 2009-11-07 | Ｔｈｄ 프라이머 타겟 검출 |
| PCT/KR2009/007064 WO2011055875A1 (en) | 2009-11-07 | 2009-11-28 | Thd primer target detection |
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| ES2533793T3 true ES2533793T3 (es) | 2015-04-14 |
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| ES09851131.4T Active ES2533793T3 (es) | 2009-11-07 | 2009-11-28 | Procedimientos de detección de diana con cebador HDD |
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| US (1) | US9328377B2 (OSRAM) |
| EP (1) | EP2496709B1 (OSRAM) |
| JP (1) | JP5770738B2 (OSRAM) |
| KR (2) | KR20110050327A (OSRAM) |
| CN (1) | CN102782150B (OSRAM) |
| AU (1) | AU2009355009B8 (OSRAM) |
| CA (1) | CA2790153C (OSRAM) |
| ES (1) | ES2533793T3 (OSRAM) |
| WO (1) | WO2011055875A1 (OSRAM) |
Families Citing this family (14)
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| WO2011027966A2 (en) * | 2009-09-03 | 2011-03-10 | Seegene, Inc. | Td probe and its uses |
| US9447457B2 (en) * | 2009-09-24 | 2016-09-20 | Seegene, Inc. | Detection of target nucleic acid sequences by cyclic exonucleolytic reactions |
| BR112012018394B8 (pt) * | 2009-12-21 | 2021-07-27 | Seegene Inc | método para detecção de uma sequência de ácido nucleico alvo e kit para detecção de uma sequência de ácido nucleico alvo |
| WO2011078439A1 (en) * | 2009-12-24 | 2011-06-30 | Seegene, Inc. | Real-time multiplexing detection of target nucleic acid sequences with elimination of false signals |
| US9074249B2 (en) | 2012-06-04 | 2015-07-07 | New England Biolabs, Inc. | Detection of amplification products |
| US9074243B2 (en) | 2012-07-27 | 2015-07-07 | New England Biolabs, Inc. | Detection of amplification products |
| KR102222546B1 (ko) * | 2013-02-07 | 2021-03-08 | 러트거즈,더스테이트유니버시티오브뉴저지 | 고도로 선택적인 핵산 증폭 프라이머 |
| WO2016137031A1 (ko) * | 2015-02-25 | 2016-09-01 | (주)다이오진 | 덤벨 구조 올리고뉴클레오티드, 이를 포함한 핵산 증폭용 프라이머 및 이를 이용한 핵산 증폭 방법 |
| CN105368943B (zh) * | 2015-11-21 | 2018-10-16 | 中国人民解放军第三〇九医院 | 一种用于鉴定分枝杆菌菌种的试剂盒及方法 |
| EP3397765B1 (en) * | 2015-12-30 | 2023-02-01 | Bio-Rad Laboratories, Inc. | Method for quantitating the frequency of wild-type and mutant fragments in a nucleic acid sample |
| CN107937607B (zh) * | 2017-11-30 | 2020-12-01 | 东北农业大学 | 用于猪传染性胃肠炎病毒检测的dpo引物组、含有该引物组的试剂盒及其应用 |
| CN108060269B (zh) * | 2018-01-19 | 2020-12-01 | 东北农业大学 | 用于猪流行性腹泻病毒、猪传染性胃肠炎病毒以及猪轮状病毒检测的dpo引物组及其应用 |
| WO2022061593A1 (zh) * | 2020-09-23 | 2022-03-31 | 迈克生物股份有限公司 | 检测核酸的组合、方法及试剂盒 |
| WO2024054924A1 (en) * | 2022-09-08 | 2024-03-14 | Gen-Probe Incorporated | Method of detecting nucleic acid analytes using dual-specificity primers |
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| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4351760A (en) | 1979-09-07 | 1982-09-28 | Syva Company | Novel alkyl substituted fluorescent compounds and polyamino acid conjugates |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| JP2846018B2 (ja) | 1988-01-21 | 1999-01-13 | ジェネンテク，インコーポレイテッド | 核酸配列の増幅および検出 |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| DE69838210T2 (de) | 1997-12-15 | 2008-05-15 | Csl Behring Gmbh | Markierter Primer, geeignet für die Detektion von Nukleinsäuren |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| US6322980B1 (en) * | 1999-04-30 | 2001-11-27 | Aclara Biosciences, Inc. | Single nucleotide detection using degradation of a fluorescent sequence |
| US7344830B2 (en) * | 2000-09-26 | 2008-03-18 | Health Research Inc. | Heteroduplex tracking assay |
| EP1439222A1 (en) * | 2001-10-26 | 2004-07-21 | Matsushita Electric Industrial Co., Ltd. | Method of detecting target nucleic acid and nucleic acid probe |
| US6818420B2 (en) * | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
| JP3923917B2 (ja) * | 2003-03-26 | 2007-06-06 | 株式会社東芝 | ターゲットの製造方法、標的配列検出方法、ターゲットおよび標的配列検出用アッセイキット |
| EP1502958A1 (en) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | New detection format for hot start real time polymerase chain reaction |
| WO2006095941A1 (en) * | 2005-03-05 | 2006-09-14 | Seegene, Inc. | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide |
| KR101243266B1 (ko) | 2005-03-05 | 2013-03-25 | 주식회사 씨젠 | 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드 |
| WO2007011946A2 (en) * | 2005-07-15 | 2007-01-25 | Applera Corporation | Detection of nucleic acid amplification |
| ES2420831T3 (es) * | 2005-07-25 | 2013-08-27 | Alere San Diego, Inc. | Procedimientos para multiplexar la ampliación de la recombinasa polimerasa |
| KR20070052890A (ko) * | 2005-11-18 | 2007-05-23 | 주식회사 씨젠 | 호흡기 바이러스 핵산 검출용 올리고뉴클레오타이드 |
| WO2011027966A2 (en) * | 2009-09-03 | 2011-03-10 | Seegene, Inc. | Td probe and its uses |
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2009
- 2009-11-07 KR KR1020090107262A patent/KR20110050327A/ko active Pending
- 2009-11-28 ES ES09851131.4T patent/ES2533793T3/es active Active
- 2009-11-28 JP JP2012537789A patent/JP5770738B2/ja active Active
- 2009-11-28 WO PCT/KR2009/007064 patent/WO2011055875A1/en not_active Ceased
- 2009-11-28 CA CA2790153A patent/CA2790153C/en active Active
- 2009-11-28 AU AU2009355009A patent/AU2009355009B8/en active Active
- 2009-11-28 EP EP09851131.4A patent/EP2496709B1/en active Active
- 2009-11-28 US US13/508,086 patent/US9328377B2/en active Active
- 2009-11-28 KR KR1020127014773A patent/KR101404130B1/ko active Active
- 2009-11-28 CN CN200980163265.0A patent/CN102782150B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102782150A (zh) | 2012-11-14 |
| KR20120084320A (ko) | 2012-07-27 |
| JP2013509871A (ja) | 2013-03-21 |
| AU2009355009A8 (en) | 2014-05-22 |
| WO2011055875A1 (en) | 2011-05-12 |
| EP2496709A4 (en) | 2013-03-27 |
| EP2496709A1 (en) | 2012-09-12 |
| AU2009355009B8 (en) | 2014-05-22 |
| EP2496709B1 (en) | 2015-01-07 |
| CA2790153A1 (en) | 2011-05-12 |
| JP5770738B2 (ja) | 2015-08-26 |
| US20120219955A1 (en) | 2012-08-30 |
| CA2790153C (en) | 2017-07-11 |
| KR20110050327A (ko) | 2011-05-13 |
| AU2009355009B2 (en) | 2014-05-08 |
| US9328377B2 (en) | 2016-05-03 |
| AU2009355009A1 (en) | 2012-05-31 |
| CN102782150B (zh) | 2015-04-01 |
| KR101404130B1 (ko) | 2014-06-10 |
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