ES2493765T3 - Sistemas y métodos para el análisis automatizado de células y tejidos - Google Patents

Sistemas y métodos para el análisis automatizado de células y tejidos Download PDF

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ES2493765T3
ES2493765T3 ES02739157.2T ES02739157T ES2493765T3 ES 2493765 T3 ES2493765 T3 ES 2493765T3 ES 02739157 T ES02739157 T ES 02739157T ES 2493765 T3 ES2493765 T3 ES 2493765T3
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image
defined area
pixel
pixel location
intensity
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David L. Rimm
Robert L. Camp
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Yale University
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    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G06T7/0014—Biomedical image inspection using an image reference approach
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/40—Analysis of texture
    • G06T7/41—Analysis of texture based on statistical description of texture
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20036—Morphological image processing
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20092—Interactive image processing based on input by user
    • G06T2207/20101—Interactive definition of point of interest, landmark or seed
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20221—Image fusion; Image merging
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30072—Microarray; Biochip, DNA array; Well plate
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30096—Tumor; Lesion

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  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Health & Medical Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Multimedia (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Medical Informatics (AREA)
  • Quality & Reliability (AREA)
  • Radiology & Medical Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Optics & Photonics (AREA)
  • Probability & Statistics with Applications (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Image Analysis (AREA)
  • Image Processing (AREA)

Abstract

Un método puesto en práctica por ordenador para localizar y cuantificar un biomarcador particular en muestras de tejido que contienen células, y el método comprende: a) usar un dispositivo óptico de formación de imágenes para obtener: i) una primera imagen de una célula con una primera tinción que es selectiva para una primera área definida dentro de la célula; ii) una segunda imagen de la célula con una segunda tinción que es selectiva para un biomarcador; y iii) una tercera imagen de la célula con una tercera tinción que es selectiva para una segunda área definida dentro de la célula, en la que cada imagen está compuesta de localizaciones de píxeles; b) leer la intensidad de píxel en cada localización de píxel de la primera imagen y la tercera imagen y comparar cada valor de intensidad con un valor de intensidad umbral predeterminado; c) asignar cada localización de píxel en la primera imagen y la tercera imagen a la primera área definida, la segunda área definida, o una tercera área definida, en el que, para cada localización de píxel, si el valor de intensidad de píxel en la localización de píxel solamente en la primera imagen es mayor que el valor de intensidad umbral respectivo, la localización de píxel se asigna a la primera área definida, si el valor de intensidad de píxel en la localización de píxel solamente en la tercera imagen es mayor que el valor de intensidad umbral respectivo, la localización de píxel se asigna a la segunda área definida, si los valores de intensidad de píxel en la localización de píxel tanto en la primera imagen como en la tercera imagen están por debajo de los valores de intensidad umbral respectivos, la localización de píxel se asigna a la tercera área definida, y si los valores de intensidad de píxel en la localización de píxel tanto en la primera imagen como en la tercera imagen son mayores que los valores de intensidad umbral respectivos, los valores de intensidad de píxel se comparan y la localización de píxel se asigna al área definida que corresponde a la imagen que tiene el mayor valor de intensidad de píxel en la localización de píxel; d) determinar la cantidad de intensidad de la primera área definida incorrectamente asignada a la segunda área definida y la cantidad de intensidad de la segunda área definida incorrectamente asignada a la primera área definida y reasignar las localizaciones de píxeles a la primera área definida o la segunda área definida respecto de una proporción ponderada de intensidad de la primera área definida respecto de la segunda área definida, y la ponderación se determina mediante un factor que se incrementa de manera iterativa hasta que la cantidad de localizaciones de píxeles incorrectamente asignadas es menor del 5%; e) comparar la segunda imagen con la primera y la tercera imágenes para identificar las localizaciones de píxeles en la segunda imagen que están dentro de la primera área definida o la segunda área definida; y f) sumar los valores de intensidad de las localizaciones de píxeles en la segunda imagen asignadas a áreas definidas similares para localizar y cuantificar así el biomarcador en las muestras de tejido que contienen células.

Description

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Claims (1)

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ES02739157.2T 2001-04-20 2002-04-19 Sistemas y métodos para el análisis automatizado de células y tejidos Expired - Lifetime ES2493765T3 (es)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US28515501P 2001-04-20 2001-04-20
US285155P 2001-04-20
US33472301P 2001-10-31 2001-10-31
US334723P 2001-10-31
US62308 2002-02-01
US10/062,308 US7219016B2 (en) 2001-04-20 2002-02-01 Systems and methods for automated analysis of cells and tissues
PCT/US2002/012084 WO2002086498A1 (en) 2001-04-20 2002-04-19 Systems and methods for automated analysis of cells and tissues

Publications (1)

Publication Number Publication Date
ES2493765T3 true ES2493765T3 (es) 2014-09-12

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ES02739157.2T Expired - Lifetime ES2493765T3 (es) 2001-04-20 2002-04-19 Sistemas y métodos para el análisis automatizado de células y tejidos

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US (8) US7219016B2 (es)
EP (1) EP1390747B1 (es)
JP (4) JP4433674B2 (es)
AU (1) AU2002311827B9 (es)
CA (1) CA2442604C (es)
ES (1) ES2493765T3 (es)
WO (1) WO2002086498A1 (es)

Families Citing this family (200)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5161052B2 (ja) * 2008-12-04 2013-03-13 オリンパス株式会社 顕微鏡システム、標本観察方法およびプログラム
US7391929B2 (en) * 2000-02-11 2008-06-24 Sony Corporation Masking tool
JP2004526154A (ja) * 2001-03-12 2004-08-26 モノジェン インコーポレイテッド 細胞ベースの検出および病態の判別
US20030190602A1 (en) * 2001-03-12 2003-10-09 Monogen, Inc. Cell-based detection and differentiation of disease states
US7219016B2 (en) 2001-04-20 2007-05-15 Yale University Systems and methods for automated analysis of cells and tissues
USRE44031E1 (en) 2001-05-10 2013-02-26 Battelle Energy Alliance, Llc Antibody profiling sensitivity through increased reporter antibody layering
US6989276B2 (en) * 2001-05-10 2006-01-24 Battelle Energy Alliance, Llc Rapid classification of biological components
US7695919B2 (en) * 2001-05-10 2010-04-13 Battelle Energy Alliance, Llc Antibody profiling sensitivity through increased reporter antibody layering
USRE46351E1 (en) 2001-05-10 2017-03-28 Battelle Energy Alliance, Llc Antibody profiling sensitivity through increased reporter antibody layering
GB0130862D0 (en) * 2001-12-22 2002-02-06 P W Allen & Company Ltd Method of examining potential cellular abnormalities
KR100769203B1 (ko) 2002-06-18 2007-10-22 가부시키가이샤 아마다 프레스 기계의 서보 드라이브 시스템 및 연속 가공 시스템
GB0229734D0 (en) * 2002-12-23 2003-01-29 Qinetiq Ltd Grading oestrogen and progesterone receptors expression
WO2004096984A2 (en) * 2003-04-25 2004-11-11 Janssen Pharmaceutica N.V. Preservation of rna in a biological sample
JP2004347454A (ja) * 2003-05-22 2004-12-09 Mitsubishi Rayon Co Ltd シェーディング補正方法
US20050136509A1 (en) 2003-09-10 2005-06-23 Bioimagene, Inc. Method and system for quantitatively analyzing biological samples
US8068988B2 (en) * 2003-09-08 2011-11-29 Ventana Medical Systems, Inc. Method for automated processing of digital images of tissue micro-arrays (TMA)
US7805003B1 (en) 2003-11-18 2010-09-28 Adobe Systems Incorporated Identifying one or more objects within an image
CA2566007C (en) 2004-05-03 2013-09-24 Hermes Biosciences, Inc. Drug delivery liposomes containing anionic polyols or anionic sugars
WO2005114578A1 (en) * 2004-05-21 2005-12-01 Bioimagene, Inc. Method and system for automated quantitation of tissue micro-array (tma) digital image analysis
AU2005285246B2 (en) * 2004-09-03 2011-07-21 The Scripps Research Institute Isolated lineage negative hematopoietic stem cells and methods of treatment therewith
EP1811017B1 (en) * 2004-11-09 2013-01-09 Kaneka Corporation Cell cultivating device, image processing device and cell detecting system
US8116984B2 (en) * 2004-11-19 2012-02-14 Trillium Diagnostics, Llc Software integrated cytometric assay for quantification of the human polymorphonuclear leukocyte FcγRI receptor (CD64)
WO2006133460A2 (en) * 2005-06-09 2006-12-14 Yale University Methods for diagnosing and treating breast cancer based on a her/er ratio
ATE509329T1 (de) * 2005-11-10 2011-05-15 Microsoft Corp Entdeckung biologischer merkmale unter verwendung zusammengesetzter bilder
WO2007074923A1 (ja) * 2005-12-27 2007-07-05 Olympus Corporation 発光測定装置並びに発光測定方法
EP1984737A2 (en) * 2006-01-17 2008-10-29 Cellumen, Inc. Method for predicting biological systems responses
US8131112B1 (en) * 2006-01-23 2012-03-06 Sandia Corporation Method for detecting cancer in a single cell using mitochondrial correlation microscopy
US20070191675A1 (en) * 2006-02-13 2007-08-16 Joel Gerardo Diaz Sanchez Actinic light colposcope and method to detect lesions in the lower female genital tract produced by human papilloma virus using an actinic light colposcope
WO2007145704A2 (en) 2006-04-24 2007-12-21 Gloucester Pharmaceuticals Gemcitabine combination therapy
CA2650776A1 (en) 2006-05-05 2007-11-15 Yale University Immunohistochemical methods for determining signal transduction activity in tumors
US8114615B2 (en) 2006-05-17 2012-02-14 Cernostics, Inc. Method for automated tissue analysis
WO2007139895A2 (en) * 2006-05-24 2007-12-06 Cellumen, Inc. Method for modeling a disease
WO2007146730A2 (en) 2006-06-08 2007-12-21 Gloucester Pharmaceuticals Deacetylase inhibitor therapy
WO2007144386A1 (de) * 2006-06-14 2007-12-21 Evotec Technologies Gmbh Verfahren zur aufnahme und auswertung von bildsequenzen
AU2007269654B2 (en) * 2006-06-30 2013-01-10 University Of Southern California Quantifiable internal reference standards for immunohistochemistry and uses thereof
WO2008008500A2 (en) * 2006-07-13 2008-01-17 Yale University Methods for making cancer prognoses based on the subcellular localization of biomarkers
US8060348B2 (en) * 2006-08-07 2011-11-15 General Electric Company Systems for analyzing tissue samples
US20080032321A1 (en) * 2006-08-07 2008-02-07 General Electric Company System and methods for analyzing images of tissue samples
US8131476B2 (en) * 2006-08-07 2012-03-06 General Electric Company System and method for co-registering multi-channel images of a tissue micro array
WO2008030381A2 (en) * 2006-09-05 2008-03-13 Veridex, Llc Methods for ranking cellular images
JP5021254B2 (ja) * 2006-09-06 2012-09-05 オリンパス株式会社 顕微鏡装置の制御方法、顕微鏡装置
EP2095119A2 (en) * 2006-11-10 2009-09-02 Cellumen, Inc. Protein-protein interaction biosensors and methods of use thereof
US7629125B2 (en) * 2006-11-16 2009-12-08 General Electric Company Sequential analysis of biological samples
US9201063B2 (en) * 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
US9697582B2 (en) 2006-11-16 2017-07-04 Visiopharm A/S Methods for obtaining and analyzing images
US7741045B2 (en) * 2006-11-16 2010-06-22 General Electric Company Sequential analysis of biological samples
US9008378B2 (en) 2006-12-20 2015-04-14 The Board Of Trustees Of The Leland Stanford Junior University Arrangement and imaging of biological samples
EP2109856B1 (en) * 2007-02-05 2019-01-16 Siemens Healthcare Diagnostics Inc. System and method for cell analysis in microscopy
JP5663873B2 (ja) * 2007-03-01 2015-02-04 日本電気株式会社 乳癌病理画像診断支援システム、乳癌病理画像診断支援方法、乳癌病理画像診断支援プログラム、及び、乳癌病理画像診断支援プログラムを記録した記録媒体
WO2008143849A2 (en) * 2007-05-14 2008-11-27 Historx, Inc. Compartment segregation by pixel characterization using image data clustering
US20080286881A1 (en) * 2007-05-14 2008-11-20 Apel William A Compositions and methods for combining report antibodies
US8351674B2 (en) * 2007-05-31 2013-01-08 Battelle Energy Alliance, Llc Image portion identification methods, image parsing methods, image parsing systems, and articles of manufacture
WO2008156669A1 (en) * 2007-06-15 2008-12-24 Historx, Inc. Method and system for standardizing microscope instruments
WO2009005715A1 (en) * 2007-06-29 2009-01-08 Yale University Methods for a predictive diagnostic test for tamoxifen
WO2009005816A1 (en) * 2007-07-03 2009-01-08 Yale University Methods for differential diagnosis of melanocytic lesions
CA2604317C (en) * 2007-08-06 2017-02-28 Historx, Inc. Methods and system for validating sample images for quantitative immunoassays
CA2596204C (en) * 2007-08-07 2019-02-26 Historx, Inc. Method and system for determining an optimal dilution of a reagent
WO2009029810A1 (en) * 2007-08-31 2009-03-05 Historx, Inc. Automatic exposure time selection for imaging tissue
JP5040597B2 (ja) * 2007-11-06 2012-10-03 日本電気株式会社 評価システム、評価方法および評価プログラム
US8031924B2 (en) * 2007-11-30 2011-10-04 General Electric Company Methods and systems for removing autofluorescence from images
US8139831B2 (en) * 2007-12-06 2012-03-20 Siemens Aktiengesellschaft System and method for unsupervised detection and gleason grading of prostate cancer whole mounts using NIR fluorscence
CN103033922A (zh) * 2008-01-02 2013-04-10 加利福尼亚大学董事会 高数值孔径远程显微镜设备
US8070398B2 (en) * 2008-02-19 2011-12-06 Irwin Industrial Tool Company Multi-blade self feed bit
US8189884B2 (en) * 2008-03-26 2012-05-29 General Electric Company Methods for assessing molecular expression of subcellular molecules
US8135187B2 (en) 2008-03-26 2012-03-13 General Electric Company Method and apparatus for removing tissue autofluorescence
EP2327040B1 (en) * 2008-08-15 2013-12-18 Visiopharm A/s A method and a system for determining a target in a biological sample by image analysis
EP2335221B8 (en) 2008-09-16 2016-05-25 Novartis AG Reproducible quantification of biomarker expression
US8729239B2 (en) 2009-04-09 2014-05-20 Nuclea Biotechnologies, Inc. Antibodies against fatty acid synthase
US8685891B2 (en) 2009-08-27 2014-04-01 Nuclea Biotechnologies, Inc. Method and assay for determining FAS expression
US8969009B2 (en) * 2009-09-17 2015-03-03 Vicki S. Thompson Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual
US9410965B2 (en) * 2009-09-17 2016-08-09 Battelle Energy Alliance, Llc Identification of discriminant proteins through antibody profiling, methods and apparatus for identifying an individual
US9677125B2 (en) * 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
US9001200B2 (en) 2010-01-12 2015-04-07 Bio-Rad Laboratories, Inc. Cell characterization using multiple focus planes
US9566047B2 (en) * 2010-02-25 2017-02-14 Erez Nevo Cryogenic biopsy system and method
US10702251B2 (en) 2010-02-25 2020-07-07 Robin Medical Inc. Cryogenic biopsy system and method
AU2011224429B2 (en) * 2010-03-10 2014-09-04 Beckman Coulter, Inc. Generating pulse parameters in a particle analyzer
NZ605526A (en) 2010-07-12 2015-04-24 Celgene Corp Romidepsin solid forms and uses thereof
EP2601513B1 (en) 2010-08-05 2014-05-14 Cambridge Research & Instrumentation, Inc. Enhancing visual assessment of samples
US8859502B2 (en) 2010-09-13 2014-10-14 Celgene Corporation Therapy for MLL-rearranged leukemia
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
WO2012065117A2 (en) * 2010-11-12 2012-05-18 William Marsh Rice University Oral cancer point of care diagnostics
WO2012075377A1 (en) 2010-12-03 2012-06-07 Historx, Inc. Identification of cancer patients likely to benefit from radiation therapy
US9092850B2 (en) * 2011-01-21 2015-07-28 Carnegie Mellon University Identifying location biomarkers
KR20120102447A (ko) * 2011-03-08 2012-09-18 삼성전자주식회사 진단장치 및 방법
EP3553527A1 (en) 2011-03-17 2019-10-16 Cernostics, Inc. Systems and compositions for diagnosing barrett's esophagus and methods of using the same
JP5372068B2 (ja) * 2011-05-20 2013-12-18 キヤノン株式会社 撮像システム、画像処理装置
EP2769225A4 (en) * 2011-10-17 2015-06-10 Nuclea Biotechnologies Inc PREDICTIVE BIOMARKERS FOR BREAST CANCER
MX342735B (es) 2011-10-24 2016-10-07 Halozyme Inc Diagnostico acompañante para terapia con agente anti-hialuronano y metodos de uso del mismo.
SG11201402711SA (en) 2011-11-29 2014-06-27 Genentech Inc Compositions and methods for prostate cancer analysis
CA2867144C (en) 2012-03-27 2019-07-02 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
WO2013148458A1 (en) 2012-03-30 2013-10-03 Clarient Diagnostic Services, Inc. Methods for generating an image of a biological sample
JP5924406B2 (ja) * 2012-04-23 2016-05-25 日本電気株式会社 画像計測装置、画像計測方法、および、画像計測用プログラム
JP5870851B2 (ja) * 2012-05-29 2016-03-01 ソニー株式会社 情報処理装置、情報処理方法、及びプログラム
AU2013202947B2 (en) 2012-06-13 2016-06-02 Ipsen Biopharm Ltd. Methods for treating pancreatic cancer using combination therapies comprising liposomal irinotecan
US9717724B2 (en) 2012-06-13 2017-08-01 Ipsen Biopharm Ltd. Methods for treating pancreatic cancer using combination therapies
AU2013277097A1 (en) * 2012-06-20 2015-01-22 Merrimack Pharmaceuticals, Inc. Marker quantitation in single cells in tissue sections
AU2013202506B2 (en) 2012-09-07 2015-06-18 Celgene Corporation Resistance biomarkers for hdac inhibitors
US9378407B2 (en) 2012-09-11 2016-06-28 Neogenomics Laboratories, Inc. Automated fish reader using learning machines
WO2014062856A1 (en) 2012-10-16 2014-04-24 Halozyme, Inc. Hypoxia and hyaluronan and markers thereof for diagnosis and monitoring of diseases and conditions and related methods
AU2013202507B9 (en) 2012-11-14 2015-08-13 Celgene Corporation Inhibition of drug resistant cancer cells
DK2927311T3 (da) * 2012-11-28 2020-01-13 Japan Science & Tech Agency Celleovervågningsindretning, celleovervågningsfremgangsmåde og program dertil
US10778913B2 (en) 2013-03-12 2020-09-15 Ventana Medical Systems, Inc. Digitally enhanced microscopy for multiplexed histology
WO2014145181A1 (en) 2013-03-15 2014-09-18 Morphotek, Inc. Methods for determining prognosis of colorectal cancer
US9064304B2 (en) 2013-03-18 2015-06-23 General Electric Company Image quality assessment of microscopy images
WO2014172345A2 (en) * 2013-04-15 2014-10-23 Massachusetts Institute Of Technology Fully automated system and method for image segmentation and quality control of protein microarrays
US10954489B2 (en) 2013-06-04 2021-03-23 The Regents Of The University Of California Liver-mimetic device and method for simulation of hepatic function using such device
EP3696277B2 (en) 2013-07-30 2025-09-03 President and Fellows of Harvard College Quantitative dna-based imaging and super-resolution imaging
EP3039477B1 (en) 2013-08-26 2021-10-20 S.D. Sight Diagnostics Ltd. Digital microscopy systems, methods and computer program products
WO2015052128A1 (en) * 2013-10-07 2015-04-16 Ventana Medical Systems, Inc. Systems and methods for comprehensive multi-assay tissue analysis
GB201318598D0 (en) * 2013-10-21 2013-12-04 Univ Leicester Improvements in or relating to super-resolution microscopy
NZ630311A (en) 2013-12-27 2016-03-31 Celgene Corp Romidepsin formulations and uses thereof
EP3099325A4 (en) * 2014-01-31 2017-10-04 The General Hospital Corporation Dba Massachusetts General Hospital Methods of treating and imaging tumor micrometastases using photoactivatable immunoconjugates
WO2015124777A1 (en) * 2014-02-21 2015-08-27 Ventana Medical Systems, Inc. Medical image analysis for identifying biomarker-positive tumor cells
JP6605506B2 (ja) * 2014-02-24 2019-11-13 ヴェンタナ メディカル システムズ， インク． ＣＤ３、ＣＤ８、ＣＤ２０及びＦｏｘＰ３の同時検出によりがんに対する免疫応答をスコア化するための方法、キット、及びシステム
EP4030166B1 (en) 2014-03-11 2023-06-28 President and Fellows of Harvard College High-throughput and highly multiplexed imaging with programmable nucleic acid probes
US20170016911A1 (en) * 2014-03-24 2017-01-19 Konica Minolta, Inc. Method for quantifying biological material based on multiple immunostaining
CN106461540B (zh) * 2014-05-12 2020-02-14 赛洛米克斯股份有限公司 发色团标记样本的自动成像的系统和方法
PL3186281T3 (pl) 2014-08-28 2019-10-31 Halozyme Inc Terapia skojarzona enzymem rozkładającym hialuronian i inhibitorem punktu kontrolnego odpowiedzi immunologicznej
WO2016044483A1 (en) 2014-09-16 2016-03-24 The Regents Of The University Of California Method for fabrication of microwells for controlled formation of 3-dimensional multicellular-shapes
JP6499506B2 (ja) * 2015-04-30 2019-04-10 富士フイルム株式会社 撮像装置および方法並びに撮像制御プログラム
US11318131B2 (en) 2015-05-18 2022-05-03 Ipsen Biopharm Ltd. Nanoliposomal irinotecan for use in treating small cell lung cancer
WO2017027370A1 (en) 2015-08-07 2017-02-16 President And Fellows Of Harvard College Super resolution imaging of protein-protein interactions
PL3337467T3 (pl) 2015-08-20 2021-06-14 Ipsen Biopharm Ltd. Terapia skojarzona z zastosowaniem liposomalnego irynotekanu i inhibitora parp do leczenia nowotworu
BR112018002941B1 (pt) 2015-08-21 2023-12-05 Ipsen Biopharm Ltd Uso de irinotecano lipossomal, oxaliplatina, leucovorina e 5- fluorouracil no tratamento de primeira linha de adenocarcinoma metastático do pâncreas
EP3341732B1 (en) 2015-08-27 2023-07-12 INSERM - Institut National de la Santé et de la Recherche Médicale Methods for predicting the survival time of patients suffering from a lung cancer
WO2017060397A1 (en) 2015-10-09 2017-04-13 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting the survival time of subjects suffering from melanoma metastases
ES3036518T3 (en) 2015-10-16 2025-09-19 Ipsen Biopharm Ltd Stabilizing camptothecin pharmaceutical compositions
WO2017067944A1 (en) 2015-10-19 2017-04-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting the survival time of subjects suffering from triple negative breast cancer
ES2961306T3 (es) * 2015-10-23 2024-03-11 Novartis Ag Método para obtener un valor de porcentaje de positividad para un biomarcador para células seleccionadas presentes en un campo de visión
EP3365677B1 (en) * 2015-10-23 2021-03-31 Novartis AG Computer processes behind an enhanced version of aqua
CN108463727B (zh) * 2015-10-23 2021-06-18 诺华股份有限公司 用于得到细胞间空间接近度的计算机方法和系统
US20180327499A1 (en) 2015-11-13 2018-11-15 INSERM (Institut National de la Santé et de la Recherche Médicale) Anti- nkg2d single domain antibodies and uses thereof
EP4152001B1 (en) 2015-11-25 2025-10-22 Cernostics, Inc. Methods of classifying barrett's esophagus
EP3400443B1 (en) 2016-01-04 2020-09-16 INSERM (Institut National de la Santé et de la Recherche Médicale) Use of pd-1 and tim-3 as a measure for cd8+ cells in predicting and treating renal cell carcinoma
ES2924741T3 (es) 2016-01-28 2022-10-10 Inst Nat Sante Rech Med Métodos para incrementar la potencia de los inhibidores del punto de control inmunitario
EP3203413A1 (en) * 2016-02-08 2017-08-09 Ecole Polytechnique Federale De Lausanne (Epfl) Method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof
US20190292259A1 (en) 2016-05-24 2019-09-26 Inserm (Institut National De La Sante Et De La Recherche Medicale) Methods and pharmaceutical compositions for the treatment of non small cell lung cancer (nsclc) that coexists with chronic obstructive pulmonary disease (copd)
WO2018011107A1 (en) 2016-07-11 2018-01-18 INSERM (Institut National de la Santé et de la Recherche Médicale) Use of er-alpha 46 in methods and kits for assessing the status of breast cancer
WO2018046736A1 (en) 2016-09-12 2018-03-15 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting the survival time of patients suffering from cancer
JP6646552B2 (ja) * 2016-09-13 2020-02-14 株式会社日立ハイテクノロジーズ 画像診断支援装置、画像診断支援方法及び試料分析システム
WO2018083470A1 (en) 2016-11-02 2018-05-11 Ipsen Biopharm Ltd. Treating gastric cancer using combination therapies comprising liposomal irinotecan, oxaliplatin, 5-fluoruracil (and leucovorin)
CA3044048A1 (en) * 2016-11-16 2018-05-24 Nanosomix, Inc. Quantification of subpopulations of exosomes and diagnosis of neurodegenerative disorders
JP2020513796A (ja) 2016-11-16 2020-05-21 ユニバーシティ オブ ワシントンＵｎｉｖｅｒｓｉｔｙ ｏｆ Ｗａｓｈｉｎｇｔｏｎ サイクリック蛍光イメージングのためのシステムおよび方法
AU2017370751B2 (en) 2016-12-09 2023-11-09 Ultivue, Inc. Improved methods for multiplex imaging using labeled nucleic acid imaging agents
WO2018122245A1 (en) 2016-12-28 2018-07-05 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods of predicting the survival time of patients suffering from cms3 colorectal cancer
WO2018122249A1 (en) 2016-12-28 2018-07-05 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting the survival time of patients suffering from a microsatellite stable colorectal cancer
WO2019043138A1 (en) 2017-09-01 2019-03-07 INSERM (Institut National de la Santé et de la Recherche Médicale) METHOD FOR PREDICTING OUTCOME OF CANCER
JP6404425B2 (ja) * 2017-09-27 2018-10-10 株式会社日立エルジーデータストレージ 光画像計測装置
WO2019089858A2 (en) 2017-11-01 2019-05-09 Juno Therapeutics, Inc. Methods of assessing or monitoring a response to a cell therapy
EP3713963A1 (en) 2017-11-23 2020-09-30 INSERM (Institut National de la Santé et de la Recherche Médicale) A new marker for predicting the sensitivity to pi3k inhibitors
EP4285838B1 (en) 2017-12-05 2025-08-13 Devicor Medical Products, Inc. Biopsy device with applied imaging
JP7239936B2 (ja) 2018-01-30 2023-03-15 国立研究開発法人理化学研究所 マスク構造最適化装置、マスク構造最適化方法およびプログラム
EP3765085A1 (en) 2018-03-12 2021-01-20 Université de Paris Use of caloric restriction mimetics for potentiating chemo-immunotherapy for the treatment of cancers
US11515009B2 (en) 2018-03-19 2022-11-29 International Business Machines Corporation Biomarker quantification in a tissue sample
JP7084193B2 (ja) * 2018-04-10 2022-06-14 ザイオソフト株式会社 医用画像処理装置、医用画像処理方法、及び医用画像処理プログラム
WO2019197683A1 (en) 2018-04-13 2019-10-17 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting outcome and treatment of patients suffering from prostate cancer or breast cancer
WO2019207030A1 (en) 2018-04-26 2019-10-31 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting a response with an immune checkpoint inhibitor in a patient suffering from a lung cancer
CN108896363A (zh) * 2018-05-28 2018-11-27 杭州智微信息科技有限公司 一种骨髓涂片数字化的流程方法
WO2020048942A1 (en) 2018-09-04 2020-03-12 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and pharmaceutical compositions for enhancing cytotoxic t lymphocyte-dependent immune responses
JP7618950B2 (ja) 2018-09-19 2025-01-22 インサーム （インスティテュート ナショナル デ ラ サンテ エ デ ラ ルシェルシェ メディカル） 免疫チェックポイント治療に抵抗性のある癌の治療のための方法および医薬組成物
WO2020070053A1 (en) 2018-10-01 2020-04-09 INSERM (Institut National de la Santé et de la Recherche Médicale) Use of inhibitors of stress granule formation for targeting the regulation of immune responses
WO2020120592A1 (en) 2018-12-12 2020-06-18 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and compositions for predicting and treating melanoma
US20220047556A1 (en) 2018-12-17 2022-02-17 INSERM (Institut National de la Santé et de la Recherche Médicale) Use of sulconazole as a furin inhibitor
US20220073626A1 (en) 2019-01-03 2022-03-10 Institut National De La Santé Et De La Recheche Médicale (Inserm) Methods and pharmaceutical compositions for enhancing cd8+ t cell-dependent immune responses in subjects suffering from cancer
SG11202107572TA (en) 2019-01-10 2021-08-30 Innovacorium Inc Pharmaceutical delivery compositions and uses thereof
US20220160871A1 (en) 2019-03-29 2022-05-26 Rakuten Medical, Inc. Methods for photoimmunotherapy and related biomarkers
WO2020201362A2 (en) 2019-04-02 2020-10-08 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods of predicting and preventing cancer in patients having premalignant lesions
WO2020208060A1 (en) 2019-04-09 2020-10-15 INSERM (Institut National de la Santé et de la Recherche Médicale) Use of sk2 inhibitors in combination with immune checkpoint blockade therapy for the treatment of cancer
JP7554779B2 (ja) 2019-06-03 2024-09-20 アンスティチュート、ナシオナル、ドゥ、ラ、サンテ、エ、ドゥ、ラ、ルシェルシュ、メディカル 処置レジメンを調節する方法
WO2020245208A1 (en) 2019-06-04 2020-12-10 INSERM (Institut National de la Santé et de la Recherche Médicale) Use of cd9 as a biomarker and as a biotarget in glomerulonephritis or glomerulosclerosis
US20240301497A1 (en) 2019-10-17 2024-09-12 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for diagnosing nasal intestinal type adenocarcinomas
US10670512B1 (en) 2019-10-18 2020-06-02 Center For Quantitative Cytometry Apparatus to perform intrinsic hyper-spectral flow cytometry
US10652484B1 (en) 2019-10-18 2020-05-12 Center For Quantitative Cytometry Method to obtain data cubes of low intensity intrinsic spectral images when imaging under high illumination conditions without the use of filters
BR112022007712A2 (pt) 2019-10-22 2022-07-12 S D Sight Diagnostics Ltd Contabilização de erros em medições ópticas
CN114616458B (zh) * 2019-11-11 2025-12-02 美天施生物科技有限两合公司 系列多通道显微术
MX2022007123A (es) 2019-12-12 2022-07-11 S D Sight Diagnostics Ltd Analisis de un analito dispuesto dentro de un medio.
AU2020402284B2 (en) * 2019-12-12 2026-02-26 S.D. Sight Diagnostics Ltd Off-focus microscopic images of a sample
MX2022007119A (es) 2019-12-12 2022-07-11 S D Sight Diagnostics Ltd Deteccion de plaquetas en una muestra de sangre.
US20230076415A1 (en) 2020-01-17 2023-03-09 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and compositions for treating melanoma
EP4110955A1 (en) 2020-02-28 2023-01-04 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for diagnosing, prognosing and managing treatment of breast cancer
US11551360B2 (en) 2020-04-28 2023-01-10 QATAR UNIVERSITY, Office of Academic Research Device and method for cancer detection
CN115698710A (zh) * 2020-05-20 2023-02-03 罗氏血液诊断股份有限公司 使用自动化平台制备打印在载玻片上的生物标志物和罗曼诺夫斯基型染色的样品
WO2021241061A1 (ja) * 2020-05-29 2021-12-02 ソニーグループ株式会社 情報処理装置、情報処理方法、コンピュータプログラム、およびターゲット分子検出システム
US20230235408A1 (en) 2020-06-30 2023-07-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting the risk of recurrence and/or death of patients suffering from a solid cancer after preoperative adjuvant therapies
JP7741831B2 (ja) 2020-06-30 2025-09-18 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 術前補助療法及び根治手術後の固形がんを患っている患者の再発及び／又は死亡のリスクを予測するための方法
CN112201300B (zh) * 2020-10-23 2022-05-13 天津大学 基于深度图像特征和阈值学习策略的蛋白质亚细胞定位的方法
KR20240012352A (ko) 2021-03-23 2024-01-29 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) T 세포 림프종의 진단 및 치료 방법
EP4330676A1 (en) 2021-04-27 2024-03-06 Institut National de la Santé et de la Recherche Médicale (INSERM) Methods for predicting the risk of lymph node metastasis and/or recurrence of patients suffering from a t1 cancer treated by endoscopic resection
IL311201A (en) * 2021-11-17 2024-05-01 Becton Dickinson Co Methods for dynamic real-time adjustment of a data acquisition parameter in a flow cytometer
EP4472740A1 (en) 2022-01-31 2024-12-11 INSERM (Institut National de la Santé et de la Recherche Médicale) Cd38 as a biomarker and biotarget in t-cell lymphomas
AU2022447608A1 (en) 2022-03-17 2024-09-26 Assistance Publique - Hôpitaux De Paris Methods for predicting response to an immunotherapeutic treatment in a patient with a cancer
EP4508432A1 (en) 2022-04-11 2025-02-19 Institut National de la Santé et de la Recherche Médicale Methods for the diagnosis and treatment of t-cell malignancies
WO2023198874A1 (en) 2022-04-15 2023-10-19 Institut National de la Santé et de la Recherche Médicale Methods for the diagnosis and treatment of t cell-lymphomas
WO2024003310A1 (en) 2022-06-30 2024-01-04 Institut National de la Santé et de la Recherche Médicale Methods for the diagnosis and treatment of acute lymphoblastic leukemia
EP4558823A1 (en) 2022-07-22 2025-05-28 Institut National de la Santé et de la Recherche Médicale Garp as a biomarker and biotarget in t-cell malignancies
WO2024023283A1 (en) 2022-07-29 2024-02-01 Institut National de la Santé et de la Recherche Médicale Lrrc33 as a biomarker and biotarget in cutaneous t-cell lymphomas
EP4602372A1 (en) 2022-10-12 2025-08-20 Institut National de la Santé et de la Recherche Médicale Cd81 as a biomarker and biotarget in t-cell malignancies
WO2024153636A1 (en) 2023-01-17 2024-07-25 Institut National de la Santé et de la Recherche Médicale Vasorin as a biomarker and biotarget in nephrology
WO2025045894A1 (en) 2023-08-28 2025-03-06 Institut National de la Santé et de la Recherche Médicale Methods and kits for diagnosing cause of nephrotic syndrome and guiding therapy
WO2026013072A1 (en) 2024-07-09 2026-01-15 Institut National de la Santé et de la Recherche Médicale Methods for predicting the health state of a subject from fibroblasts

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US26420A (en) 1859-12-13 Improvement in seeding-machines
US4998284A (en) * 1987-11-17 1991-03-05 Cell Analysis Systems, Inc. Dual color camera microscope and methodology for cell staining and analysis
JP2628302B2 (ja) * 1987-04-14 1997-07-09 富士レビオ株式会社 凝集判定方法および装置
JP2852096B2 (ja) * 1990-03-29 1999-01-27 オリンパス光学工業株式会社 被検体通過中の散乱光成分抑制方法およびその装置
US5386819A (en) 1990-03-29 1995-02-07 Olympus Optical Co., Ltd. Method and apparatus for inhibiting a scattered component in a light having passed through an examined object
JPH0474950A (ja) * 1990-07-16 1992-03-10 Hitachi Ltd 発光検出型遺伝物質検出装置
US5784162A (en) 1993-08-18 1998-07-21 Applied Spectral Imaging Ltd. Spectral bio-imaging methods for biological research, medical diagnostics and therapy
US5936731A (en) 1991-02-22 1999-08-10 Applied Spectral Imaging Ltd. Method for simultaneous detection of multiple fluorophores for in situ hybridization and chromosome painting
US6026174A (en) * 1992-10-14 2000-02-15 Accumed International, Inc. System and method for automatically detecting malignant cells and cells having malignancy-associated changes
US6229649B1 (en) 1994-10-04 2001-05-08 The United States Of America As Represented By The Secretary Of The Air Force Pseudo deconvolution method of recovering a distorted optical image
EP0839362A1 (en) * 1995-07-19 1998-05-06 Morphometrix Technologies Inc. Multi-spectral segmentation for image analysis
US6151405A (en) * 1996-11-27 2000-11-21 Chromavision Medical Systems, Inc. System and method for cellular specimen grading
WO1997020198A2 (en) * 1995-11-30 1997-06-05 Chromavision Medical Systems, Inc. Method and apparatus for automated image analysis of biological specimens
US5998151A (en) 1995-12-01 1999-12-07 The United States Of America As Represented By The Department Of Health And Human Services Methods for predicting the efficacy of a chemotherapeutic regimen for gastrointestinal cancers using antibodies specific for thymidylate synthase
US6165734A (en) * 1995-12-12 2000-12-26 Applied Spectral Imaging Ltd. In-situ method of analyzing cells
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US6727071B1 (en) * 1997-02-27 2004-04-27 Cellomics, Inc. System for cell-based screening
WO1999031509A1 (en) * 1997-11-27 1999-06-24 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Improved method for the identification and characterization of interacting molecules using automation
US20030036855A1 (en) * 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
KR100618502B1 (ko) * 1998-03-16 2006-09-01 지이 헬스케어 바이오-사이언시즈 코프. 공초점 마이크로스코피 영상 시스템에서 사용하기 위한 전자기 방출의 집속 시스템 및 방법
US6492325B1 (en) 1998-05-22 2002-12-10 Boys Town National Research Hospital Use of α1β1 integrin receptor inhibitors and TGF-β1 inhibitors in the treatment of kidney disease
US6337472B1 (en) 1998-10-19 2002-01-08 The University Of Texas System Board Of Regents Light imaging microscope having spatially resolved images
JP5213202B2 (ja) 1998-10-28 2013-06-19 バイシス・インコーポレーテツド 細胞アレイならびに遺伝障害マーカーの検出および使用方法
US7666609B1 (en) 1998-12-01 2010-02-23 Shanghai Cp Guojian Pharmaceutical Co. Ltd. Method and composition for diagnosis of melanocytic lesions
US6351712B1 (en) * 1998-12-28 2002-02-26 Rosetta Inpharmatics, Inc. Statistical combining of cell expression profiles
US6251601B1 (en) * 1999-02-02 2001-06-26 Vysis, Inc. Simultaneous measurement of gene expression and genomic abnormalities using nucleic acid microarrays
JP2000283853A (ja) * 1999-03-31 2000-10-13 Mitsubishi Electric Corp 波面センサ
WO2000060126A2 (en) * 1999-04-08 2000-10-12 Sir Mortimer B. Davis Jewish General Hospital Quantitative assay for expression of genes in microarray
AU762085B2 (en) * 1999-04-13 2003-06-19 Chromavision Medical Systems, Inc. Histological reconstruction and automated image analysis
AU7494900A (en) 1999-09-17 2001-04-17 Government of The United States of America, as represented by The Secretary Department of Health & Human Services, The National Institutes of Health, The Signal counting for in situ hybridization
WO2001022086A1 (en) 1999-09-24 2001-03-29 Jonathan Cohen A high-throughput system for evaluating the clinical utility of molecular targets in tissue samples
US7050087B2 (en) * 2000-12-06 2006-05-23 Bioview Ltd. Data acquisition and display system and method
US6466690C1 (en) 2000-12-19 2008-11-18 Bacus Res Lab Inc Method and apparatus for processing an image of a tissue sample microarray
WO2002100157A2 (en) 2001-02-21 2002-12-19 Amnis Corporation Method and apparatus for labeling and analyzing cellular components
US7219016B2 (en) * 2001-04-20 2007-05-15 Yale University Systems and methods for automated analysis of cells and tissues
US7229774B2 (en) 2001-08-02 2007-06-12 Regents Of The University Of Michigan Expression profile of prostate cancer
FI20020653A0 (fi) 2002-04-05 2002-04-05 Taipale Automotive Oy Menetelmä ajoneuvoa ajavan henkilön sekä hänen ajotapansa tunnistamiseksi
US20060024692A1 (en) 2002-09-30 2006-02-02 Oncotherapy Science, Inc. Method for diagnosing non-small cell lung cancers
DK2447375T3 (da) 2004-03-31 2019-07-29 Massachusetts Gen Hospital Fremgangsmåde til bestemmelse af responsiviteten af en tumor på behandlinger med den epidermale vækstfaktor-receptor som mål
US7862995B2 (en) 2004-12-10 2011-01-04 Targeted Molecular Diagnostics Methods and materials for predicting responsiveness to treatment with dual tyrosine kinase inhibitor
EP1872124A4 (en) 2005-04-19 2008-06-11 Prediction Sciences Llc DIAGNOSIS MARKET FOR BREAST CANCER TREATMENT AND PROGRESSION AND METHOD OF USE THEREOF
GB0524190D0 (en) 2005-11-28 2006-01-04 Helse Stavanger Hf Method
WO2008008500A2 (en) 2006-07-13 2008-01-17 Yale University Methods for making cancer prognoses based on the subcellular localization of biomarkers
NZ599214A (en) 2007-06-01 2013-12-20 Univ California Multigene prognostic assay for lung cancer
WO2009005715A1 (en) 2007-06-29 2009-01-08 Yale University Methods for a predictive diagnostic test for tamoxifen
WO2009005816A1 (en) 2007-07-03 2009-01-08 Yale University Methods for differential diagnosis of melanocytic lesions
WO2011047019A1 (en) 2009-10-13 2011-04-21 Yale University An objective, quantitative method to predict histological subtype in non-small cell lung cancer
US20120270239A1 (en) 2009-10-30 2012-10-25 Rimm David L Melanoma prognostic model using tissue microarrays and genetic algorithms
US20140148353A1 (en) 2011-02-22 2014-05-29 Yale University Protein expression-based classifier for prediction of recurrence in adenocarcinoma

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US20120329665A1 (en) 2012-12-27
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US20140369586A1 (en) 2014-12-18
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