ES2309989T3 - Oocitos inactivados que son receptores de citoplastos para transferencia nuclear. - Google Patents
Oocitos inactivados que son receptores de citoplastos para transferencia nuclear. Download PDFInfo
- Publication number
- ES2309989T3 ES2309989T3 ES96928586T ES96928586T ES2309989T3 ES 2309989 T3 ES2309989 T3 ES 2309989T3 ES 96928586 T ES96928586 T ES 96928586T ES 96928586 T ES96928586 T ES 96928586T ES 2309989 T3 ES2309989 T3 ES 2309989T3
- Authority
- ES
- Spain
- Prior art keywords
- human
- embryo
- nucleus
- activation
- mammal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8773—Ovine embryos
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8771—Bovine embryos
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Developmental Biology & Embryology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Reproductive Health (AREA)
- Animal Behavior & Ethology (AREA)
- Environmental Sciences (AREA)
- Immunology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Gynecology & Obstetrics (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9517779 | 1995-08-31 | ||
| GBGB9517779.6A GB9517779D0 (en) | 1995-08-31 | 1995-08-31 | Biological manipulation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2309989T3 true ES2309989T3 (es) | 2008-12-16 |
Family
ID=10779997
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES96928586T Expired - Lifetime ES2309989T3 (es) | 1995-08-31 | 1996-08-30 | Oocitos inactivados que son receptores de citoplastos para transferencia nuclear. |
Country Status (22)
| Country | Link |
|---|---|
| US (12) | US6252133B1 (enExample) |
| EP (1) | EP0847237B1 (enExample) |
| JP (3) | JP2000506721A (enExample) |
| KR (4) | KR20060057638A (enExample) |
| CN (3) | CN1772891A (enExample) |
| AT (1) | ATE400176T1 (enExample) |
| AU (1) | AU728809B2 (enExample) |
| BR (1) | BR9610013A (enExample) |
| CA (1) | CA2229657A1 (enExample) |
| CZ (1) | CZ60498A3 (enExample) |
| DE (1) | DE69637591D1 (enExample) |
| DK (1) | DK0847237T3 (enExample) |
| ES (1) | ES2309989T3 (enExample) |
| GB (1) | GB9517779D0 (enExample) |
| HU (1) | HUP9802485A3 (enExample) |
| IL (1) | IL123417A0 (enExample) |
| NO (1) | NO980846L (enExample) |
| NZ (2) | NZ316148A (enExample) |
| PL (1) | PL325336A1 (enExample) |
| SG (1) | SG75919A1 (enExample) |
| WO (1) | WO1997007668A1 (enExample) |
| ZA (1) | ZA967383B (enExample) |
Families Citing this family (193)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5480772A (en) | 1993-02-03 | 1996-01-02 | Brandeis University | In vitro activation of a nucleus |
| GB9517780D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| US7307198B2 (en) | 1995-08-31 | 2007-12-11 | Roslin Institute | Ungulates produced by nuclear transfer of G1 cells |
| GB9517779D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| US6077697A (en) * | 1996-04-10 | 2000-06-20 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| US6025155A (en) * | 1996-04-10 | 2000-02-15 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| WO1998016630A1 (en) | 1996-10-11 | 1998-04-23 | The Texas A & M University System | Methods for the generation of primordial germ cells and transgenic animal species |
| US6235969B1 (en) * | 1997-01-10 | 2001-05-22 | University Of Massachusetts | Cloning pigs using donor nuclei from non-quiescent differentiated cells |
| US5945577A (en) * | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| US20090092588A1 (en) * | 1997-01-10 | 2009-04-09 | University Of Massachusetts As Represented By Its Amherst Campus | Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease |
| US6011197A (en) | 1997-03-06 | 2000-01-04 | Infigen, Inc. | Method of cloning bovines using reprogrammed non-embryonic bovine cells |
| DE69833545T2 (de) | 1997-03-26 | 2006-12-14 | Imperial College Innovations Ltd. | In der zellmembran verankertes antikoagulant-fusionsprotein |
| JP2001509361A (ja) | 1997-07-03 | 2001-07-24 | ユニヴァーシティー オヴ マサチューセッツ | 分化した無血清飢餓細胞からの供与核を用いるクローニング |
| US7923015B2 (en) | 1997-08-14 | 2011-04-12 | Institut Pasteur | Methods for direct visualization of active synapses |
| US7923216B2 (en) | 1997-08-14 | 2011-04-12 | Institut Pasteur | In vivo modulation of neuronal transport |
| JPH11127852A (ja) * | 1997-10-28 | 1999-05-18 | Snow Brand Milk Prod Co Ltd | 上皮細胞のｇ０期同調方法 |
| AU747070B2 (en) * | 1998-01-16 | 2002-05-09 | Agrobiogen Gmbh | Efficient nuclear transfer using primordial germ cells |
| US7351876B2 (en) | 1998-01-16 | 2008-04-01 | Agrobiogen Gmbh Biotechnologie | Efficient nuclear transfer with primordial gametes |
| US6600087B1 (en) * | 1998-01-16 | 2003-07-29 | Agrobiogen Gmbh | Efficient nuclear transfer using fetal fibroblasts and multiple cloning procedures |
| US6331659B1 (en) | 1998-01-21 | 2001-12-18 | University Of Hawaii | Cumulus cells as nuclear donors |
| CA2324009A1 (en) * | 1998-03-16 | 1999-09-23 | Relag Pty Ltd. | Porcine nuclear transfer |
| AUPP294898A0 (en) * | 1998-04-15 | 1998-05-07 | Monash University | Method of nuclear transfer |
| GB9808325D0 (en) * | 1998-04-20 | 1998-06-17 | Ltr Ciz Di Associazione Italia | Source of nuclei for nuclear transfer |
| US6143564A (en) * | 1998-07-07 | 2000-11-07 | University Of Hawaii | Use of the polar body chromosomes for the production of embryos and normal offspring |
| GB9820988D0 (en) * | 1998-09-25 | 1998-11-18 | Biotech & Biolog Scien Res | Screening method |
| WO2000022098A1 (en) * | 1998-10-12 | 2000-04-20 | Geron Bio-Med Limited | Porcine oocytes with improved developmental competence |
| EP1818397A1 (en) * | 1998-11-02 | 2007-08-15 | Trustees Of Tufts College | Methods for cloning animals |
| US6781030B1 (en) | 1998-11-02 | 2004-08-24 | Trustee Of Tufts College, Ballou Hall | Methods for cloning mammals using telophase oocytes |
| NZ511299A (en) | 1998-11-19 | 2003-02-28 | Ppl Therapeutics Scotland Ltd | Stabilisation of milk from transgenic animals through expression of a serine proteinase inhibitor and optionally fibrinogen in the mammary gland |
| US6700037B2 (en) | 1998-11-24 | 2004-03-02 | Infigen, Inc. | Method of cloning porcine animals |
| US6258998B1 (en) | 1998-11-24 | 2001-07-10 | Infigen, Inc. | Method of cloning porcine animals |
| EP1141265B1 (en) * | 1999-01-13 | 2008-09-03 | Revivicor, Inc. | Double nuclear transfer method and results thereof |
| US7531715B1 (en) * | 1999-01-13 | 2009-05-12 | Ppl Therapeutics (Scotland) | Double nuclear transfer method and results thereof |
| WO2000044914A1 (en) | 1999-01-28 | 2000-08-03 | Medical College Of Georgia Research Institute, Inc. | Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna |
| AU3382800A (en) | 1999-02-26 | 2000-09-14 | Millennium Pharmaceuticals, Inc. | Secreted proteins and uses thereof |
| EP2199392A3 (en) * | 1999-03-04 | 2010-12-15 | Revivicor, Inc. | Genetic modification of somatic cells and uses thereof |
| US20020012660A1 (en) * | 1999-03-04 | 2002-01-31 | Alan Colman | Method of preparing a somatic cells for nuclear transfer |
| WO2001000855A1 (en) | 1999-06-23 | 2001-01-04 | Ppl Therapeutics (Scotland) Ltd. | Fusion proteins incorporating lysozyme |
| US7291714B1 (en) | 1999-06-30 | 2007-11-06 | Millennium Pharmaceuticals, Inc. | Glycoprotein VI and uses thereof |
| KR100368435B1 (ko) * | 1999-06-30 | 2003-01-24 | 황우석 | 체세포 복제동물의 생산을 위한 세포융합방법 |
| JP3652308B2 (ja) | 1999-06-30 | 2005-05-25 | ソウル ナショナル ユニバーシティー インダストリー ファウンデーション | クローンウシの生産方法 |
| EP1109890A4 (en) * | 1999-06-30 | 2004-12-29 | Hwang Woo Suk | PROCESS FOR OBTAINING CLONED HUMAN EMBRYOS USING INTER-SPECIES TRANSPLANTATION TECHNIQUES OF CORES |
| AU753209B2 (en) * | 1999-06-30 | 2002-10-10 | Seoul National University Industry Foundation | Method for producing cloned tigers by employing inter-species nuclear transplantation technique |
| KR100414043B1 (ko) * | 1999-06-30 | 2004-01-13 | 황우석 | 체세포 복제동물 및 그 생산방법 |
| PT1210428E (pt) | 1999-08-23 | 2015-07-21 | Genetics Inst Llc | Pd-1, um recetor para b7-4 e suas utilizações |
| EP2275559A3 (en) | 1999-09-28 | 2011-03-23 | Shire Human Genetic Therapies, Inc. | Optimized messenger RNA |
| WO2002064799A2 (en) | 1999-09-28 | 2002-08-22 | Transkaryotic Therapies, Inc. | Optimized messenger rna |
| KR100417566B1 (ko) * | 1999-11-19 | 2004-02-05 | 한국생명공학연구원 | 체세포 핵치환 복제수정란의 대량생산방법 |
| US7820878B2 (en) * | 1999-11-19 | 2010-10-26 | Kyowa Hakko Kirin Co., Ltd. | Production of ungulates, preferably bovines that produce human immunoglobulins |
| US7074983B2 (en) | 1999-11-19 | 2006-07-11 | Kirin Beer Kabushiki Kaisha | Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin |
| AU1777301A (en) * | 1999-11-19 | 2001-05-30 | Hematech, Llc | Production of ungulates, preferably bovines that produce human immunoglobulins |
| US7253334B2 (en) * | 2000-12-22 | 2007-08-07 | Aurox, Llc | Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells |
| US7414170B2 (en) * | 1999-11-19 | 2008-08-19 | Kirin Beer Kabushiki Kaisha | Transgenic bovines capable of human antibody production |
| GB9927444D0 (en) * | 1999-11-19 | 2000-01-19 | Cancer Res Campaign Tech | Inhibiting gene expression |
| CN1293188C (zh) | 2000-01-04 | 2007-01-03 | 康涅狄格州大学 | 一种将长期培养的可包括人工诱导基因改变的雌或雄体细胞的细胞核转移到去核受体细胞中实现靶向基因改变的克隆动物的方法 |
| US6635802B1 (en) * | 2000-01-10 | 2003-10-21 | The Texas A&M University System | Nuclear transfer using cells cultured in serum starvation media containing apoptosis inhibitors |
| DK1248640T3 (da) | 2000-01-20 | 2007-02-12 | Diabcell Pty Ltd | Xenotransplantation af svineöer og deres fremstilling |
| AU2001236919A1 (en) * | 2000-02-09 | 2001-08-20 | The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services | Premeiotic and postmeiotic origin of teratomas: isolated teratoma stem cells for therapeutic uses |
| WO2001068831A2 (en) | 2000-03-15 | 2001-09-20 | The University Of Georgia Research Foundation, Inc. | Effective nuclear reprogramming in mammals |
| GB2360522A (en) | 2000-03-24 | 2001-09-26 | Geron Corp | A strategy for maintaining pregnancy |
| AU2001261650A1 (en) * | 2000-05-15 | 2001-11-26 | Geron Corporation | Ovine tissue for xenotransplantation |
| ATE439453T1 (de) | 2000-06-05 | 2009-08-15 | Genetics Inst Llc | Zusammensetzungen, testsätze und methoden zur identifizierung und modulation von typ i diabetes |
| EP1320599A2 (en) | 2000-06-28 | 2003-06-25 | Genetics Institute, LLC | Pd-l2 molecules: pd-1 ligands and uses therefor |
| EP1311530A4 (en) | 2000-08-03 | 2004-10-06 | Wim-Van Schooten | PRODUCTION OF HUMANIZED ANTIBODIES IN TRANSGENIC ANIMALS |
| US6677500B2 (en) | 2000-08-15 | 2004-01-13 | Board Of Trustees Of The University Of Illinois | Ungulates expressing exogenous IGF-I in their milk |
| US7049480B1 (en) * | 2000-09-01 | 2006-05-23 | Avigenics, Inc. | Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy |
| FR2814642B1 (fr) | 2000-10-03 | 2005-07-01 | Ass Pour Le Dev De La Rech En | Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee |
| CA2425076A1 (en) * | 2000-10-06 | 2002-04-11 | Advanced Cell Technology, Inc. | Cloning endangered and extinct species |
| US6821731B2 (en) | 2000-11-28 | 2004-11-23 | Wyeth | Expression analysis of FKBP nucleic acids and polypeptides useful in the diagnosis of prostate cancer |
| WO2002044419A2 (en) | 2000-11-28 | 2002-06-06 | Wyeth | Expression analysis of kiaa nucleic acids and polypeptides useful in the diagnosis and treatment of prostate cancer |
| WO2002102997A2 (en) * | 2000-11-30 | 2002-12-27 | Stemron Inc. | Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same |
| US7491534B2 (en) | 2000-12-22 | 2009-02-17 | Kirin Holdings Kabushiki Kaisha | Methods for altering cell fate to generate T-cells specific for an antigen of interest |
| US20020142397A1 (en) * | 2000-12-22 | 2002-10-03 | Philippe Collas | Methods for altering cell fate |
| EP1217071B1 (en) | 2000-12-22 | 2010-04-28 | Institut National De La Recherche Agronomique | Position-independent and tissue specific expression of a transgene in milk of transgenic animals |
| EP1860199B1 (en) | 2001-01-12 | 2014-03-05 | Exelixis, Inc. | Modulating insulin receptor signaling |
| CA2435857A1 (en) | 2001-01-30 | 2002-09-26 | Rebholtz, Amy K. | Methods for embryo recipient management |
| CA2369944A1 (en) | 2001-01-31 | 2002-07-31 | Nucleonics Inc. | Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell |
| JP2002262716A (ja) * | 2001-03-07 | 2002-09-17 | National Agricultural Research Organization | 株化細胞を用いて家畜個体を作出する方法 |
| US8981061B2 (en) | 2001-03-20 | 2015-03-17 | Novo Nordisk A/S | Receptor TREM (triggering receptor expressed on myeloid cells) and uses thereof |
| US7126039B2 (en) * | 2001-03-21 | 2006-10-24 | Geron Corporation | Animal tissue with carbohydrate antigens compatible for human transplantation |
| US7265262B2 (en) * | 2001-03-21 | 2007-09-04 | Roslin Institute (Edinburgh) | Telomerizing nuclear donor cells and improving the efficiency on nuclear transfer |
| WO2002079499A1 (en) | 2001-04-02 | 2002-10-10 | Wyeth | Pd-1, a receptor for b7-4, and uses therefor |
| CN1636062A (zh) | 2001-04-16 | 2005-07-06 | 惠氏控股公司 | 编码多肽抗原的新肺炎链球菌可读框及其应用 |
| WO2002103350A1 (en) * | 2001-06-14 | 2002-12-27 | Infigen, Inc. | Methods for cloning mammals using remodeling factors |
| EP1406917A4 (en) * | 2001-06-15 | 2007-11-14 | New Century Pharmaceuticals | HUMAN ANALYTICAL MODELS FOR THE EVALUATION OF MEDICINAL PRODUCTS AND FOR TOXICOLOGICAL AND IMMUNOGENIC EXAMINATIONS |
| ES2609014T3 (es) | 2001-07-12 | 2017-04-18 | University Of Massachusetts | Producción in vivo de ARN de interferencia pequeños que median el silenciamiento génico |
| EP1900815B1 (en) | 2001-07-12 | 2016-09-07 | University of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
| US20030211603A1 (en) * | 2001-08-14 | 2003-11-13 | Earp David J. | Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells |
| US20030170678A1 (en) * | 2001-10-25 | 2003-09-11 | Neurogenetics, Inc. | Genetic markers for Alzheimer's disease and methods using the same |
| US20030224380A1 (en) * | 2001-10-25 | 2003-12-04 | The General Hospital Corporation | Genes and polymorphisms on chromosome 10 associated with Alzheimer's disease and other neurodegenerative diseases |
| AU2002364945A1 (en) * | 2001-10-25 | 2003-07-09 | Neurogenetics, Inc. | Genes and polymorphisms on chromosome 10 associated with alzheimer's disease and other neurodegenerative diseases |
| WO2003042362A2 (en) | 2001-11-09 | 2003-05-22 | Dana-Farber Cancer Institute, Inc. | PGC-1β, A NOVEL PGC-1 HOMOLOGUE AND USES THEREFOR |
| WO2003046129A2 (en) * | 2001-11-09 | 2003-06-05 | Trustees Of The University Of Pennsylvania | Compositions for the derivation of germ cells from stem cells and methods of use thereof |
| WO2003060083A2 (en) * | 2002-01-15 | 2003-07-24 | Advanced Cell Technology, Inc. | Cloning b and t lymphocytes |
| US20050170506A1 (en) * | 2002-01-16 | 2005-08-04 | Primegen Biotech Llc | Therapeutic reprogramming, hybrid stem cells and maturation |
| US20030134422A1 (en) * | 2002-01-16 | 2003-07-17 | Sayre Chauncey Bigelow | Stem cell maturation for all tissue lines |
| US20050090004A1 (en) * | 2003-01-16 | 2005-04-28 | Sayre Chauncey B. | Stem cell maturation for all tissue lines |
| CA2480802A1 (en) * | 2002-04-01 | 2003-10-16 | Gtc Biotherapeutics, Inc. | A method for selecting cell lines to be used for nuclear transfer in mammalian species |
| US20030217378A1 (en) * | 2002-04-05 | 2003-11-20 | The University Of Georgia Research Foundation, Inc | Cloning using rapidly matured oocytes |
| US7612250B2 (en) | 2002-07-29 | 2009-11-03 | Trustees Of Tufts College | Nuclear transfer embryo formation method |
| WO2004018999A2 (en) | 2002-08-20 | 2004-03-04 | Millenium Pharmaceuticals, Inc. | Compositions, kits, and methods for identification, assessment, prevention, and therapy of cervical cancer |
| EP1575518A4 (en) | 2002-10-10 | 2007-08-22 | Wyeth Corp | COMPOSITIONS, ORGANISMS AND METHODS WITH A NEW HUMAN KINASE |
| AU2003286442A1 (en) * | 2002-10-16 | 2004-05-04 | Advanced Cell Technology, Inc. | Methods using gene trapped stem cells for marking pathways of stem cell differentiation and making and isolating differentiated cells |
| US7208306B2 (en) | 2002-10-24 | 2007-04-24 | Wyeth | Compositions employing a novel human protein phosphatase |
| JP2006505291A (ja) | 2002-11-08 | 2006-02-16 | ヘマテック，エルエルシー | プリオンタンパク質活性が低減されたトランスジェニック有蹄動物及びその用途 |
| AU2003298689A1 (en) | 2002-11-21 | 2004-06-18 | Wyeth | Methods for diagnosing rcc and other solid tumors |
| WO2004050831A2 (en) | 2002-11-27 | 2004-06-17 | Wyeth | Compositions, organisms and methodologies employing a novel human kinase |
| US7482117B2 (en) | 2002-12-20 | 2009-01-27 | Celera Corporation | Genetic polymorphisms associated with myocardial infarction, methods of detection and uses thereof |
| DE602004015064D1 (de) | 2003-01-06 | 2008-08-28 | Wyeth Corp | Zusammensetzungen und verfahren zur diagnose und behandlung von kolonkrebs |
| ES2712695T3 (es) | 2003-06-02 | 2019-05-14 | Univ Massachusetts | Métodos y composiciones para controlar la eficacia de la silenciación del ARN |
| AU2004252442B2 (en) | 2003-06-02 | 2010-04-08 | University Of Massachusetts | Methods and compositions for enhancing the efficacy and specificity of FNAi |
| EP1654349A4 (en) | 2003-08-14 | 2008-08-20 | Exelixis Inc | SULFS AS BETA-CATENIN PATH MODIFICATORS AND METHOD OF USE |
| CN102517381A (zh) | 2003-10-07 | 2012-06-27 | 千年药品公司 | 用于鉴定、评价、预防和治疗卵巢癌的核酸分子和蛋白质 |
| WO2005046450A2 (en) | 2003-11-12 | 2005-05-26 | Children's Hospital Medical Center | Method for diagnosis and treatment of pulmonary disorders |
| CA2921196C (en) | 2003-11-26 | 2019-03-05 | Celera Corporation | Single nucleotide polymorphisms associated with cardiovascular disorders and statin response, methods of detection and uses thereof |
| US7682828B2 (en) | 2003-11-26 | 2010-03-23 | Whitehead Institute For Biomedical Research | Methods for reprogramming somatic cells |
| CA2563064A1 (en) | 2004-04-22 | 2005-11-10 | Kirin Beer Kabushiki Kaisha | Transgenic animals and uses thereof |
| EP2412826B1 (en) | 2004-05-07 | 2014-01-08 | Celera Corporation | Genetic polymorphism associated with liver fibrosis methods of detection and uses thereof |
| EP1812041A2 (en) | 2004-10-22 | 2007-08-01 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for modulating pgc-1 to treat lipid-related diseases and disorders |
| GB0426146D0 (en) | 2004-11-29 | 2004-12-29 | Bioxell Spa | Therapeutic peptides and method |
| CN1274829C (zh) | 2004-12-10 | 2006-09-13 | 四川大学华西医院 | 抗eb病毒所致肿瘤多肽及其应用与制备方法 |
| WO2006099365A2 (en) | 2005-03-11 | 2006-09-21 | Applera Corporation | Genetic polymorphisms associated with coronary heart disease, methods of detection and uses thereof |
| US20070011603A1 (en) * | 2005-07-06 | 2007-01-11 | Mikko Makela | Method, system, device and software product for showing tooltips for page segments and generating content for the page segments |
| US20090186097A1 (en) | 2005-08-09 | 2009-07-23 | David Lee Ayares | Transgenic Ungulates Expressing CTLA4-IG and Uses Thereof |
| AU2006287315B2 (en) | 2005-09-09 | 2013-02-21 | The Johns Hopkins University | Manipulation of regulatory T cell and DC function by targeting neuritin gene using antibodies, agonists and antagonists |
| CA2623106C (en) | 2005-09-19 | 2013-12-24 | Histogenics Corporation | Cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof |
| US7799530B2 (en) | 2005-09-23 | 2010-09-21 | Celera Corporation | Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof |
| US7695911B2 (en) | 2005-10-26 | 2010-04-13 | Celera Corporation | Genetic polymorphisms associated with Alzheimer's Disease, methods of detection and uses thereof |
| ES2378407T3 (es) | 2006-09-01 | 2012-04-12 | Therapeutic Human Polyclonals, Inc. | Aumento de la expresión de inmunoglobulina humana o humanizada en animales transgénicos no humanos |
| EP2639318B1 (en) | 2006-09-11 | 2015-04-08 | Celera Corporation | Genetic polymorphisms associated with Psoriasis, methods of detection and uses thereof |
| US7910303B2 (en) | 2006-10-20 | 2011-03-22 | Celera Corporation | Genetic polymorphisms associated with venous thrombosis, methods of detection and uses thereof |
| KR101398220B1 (ko) * | 2006-10-30 | 2014-05-22 | 건국대학교 산학협력단 | 형질전환 배아 생성방법 |
| US20100205678A1 (en) * | 2007-01-05 | 2010-08-12 | Overstrom Eric W | Oocyte spindle-associated factors improve somatic cell cloning |
| EP2626416A3 (en) | 2007-04-07 | 2013-12-18 | The Whitehead Institute for Biomedical Research | Reprogramming of somatic cells |
| WO2008134522A1 (en) * | 2007-04-26 | 2008-11-06 | President And Fellows Of Harvard College | Deriving embryonic stem cells |
| US7972849B2 (en) * | 2007-05-17 | 2011-07-05 | Oregon Health & Science University | Primate pluripotent stem cells produced by somatic cell nuclear transfer |
| US20090111764A1 (en) * | 2007-10-25 | 2009-04-30 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Mitochondrial selection |
| WO2009055656A2 (en) | 2007-10-26 | 2009-04-30 | Centocor, Inc. | Vectors, host cells, and methods of production and uses |
| US8039212B2 (en) | 2007-11-05 | 2011-10-18 | Celera Corporation | Genetic polymorphisms associated with liver fibrosis, methods of detection and uses thereof |
| US20090221620A1 (en) | 2008-02-20 | 2009-09-03 | Celera Corporation | Gentic polymorphisms associated with stroke, methods of detection and uses thereof |
| KR100915397B1 (ko) * | 2008-02-22 | 2009-09-03 | 삼성전자주식회사 | 커버부재, 현상 카트리지 및 화상형성장치의 현상유닛 |
| WO2009114185A2 (en) | 2008-03-12 | 2009-09-17 | The Rockefeller University | Methods and compositions for translational profiling and molecular phenotyping |
| US8288094B2 (en) | 2008-04-09 | 2012-10-16 | Institut Pasteur | APOBEC3 mediated DNA editing |
| US9497943B2 (en) | 2008-06-13 | 2016-11-22 | Whitehead Institute For Biomedical Research | Nucleic acid constructs encoding reprogramming factors linked by self-cleaving peptides |
| EP2878680B1 (en) | 2008-07-09 | 2016-06-08 | Celera Corporation | Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof |
| WO2010080985A1 (en) | 2009-01-08 | 2010-07-15 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for induced brown fat differentiation |
| US8722964B2 (en) | 2009-04-23 | 2014-05-13 | Transposagen Biopharmaceuticals, Inc. | Genetically engineered or transgenic rats exhibiting a cancer phenotype due to a disruption of germline tumor suppressor genes |
| CA2759608A1 (en) | 2009-04-24 | 2010-10-28 | Oregon Health & Science University | Methods for mitochondrial dna replacement in oocytes |
| EP2267153A1 (en) | 2009-05-26 | 2010-12-29 | Université Claude Bernard Lyon 1 | Identification of netrin-1 receptor unc5c gene mutation in solid cancers |
| EP2449112A1 (en) | 2009-07-01 | 2012-05-09 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for severe combined immunodeficiency (scid) |
| EP2456876A2 (en) | 2009-07-24 | 2012-05-30 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for cytokine-cytokine signaling pathways |
| US20110145936A1 (en) | 2009-07-30 | 2011-06-16 | Ostertag Eric M | Genetically Modified Rat Models for Pharmacokinetics |
| WO2011017518A2 (en) | 2009-08-05 | 2011-02-10 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for drug metabolism |
| EP2467013A2 (en) | 2009-08-20 | 2012-06-27 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for pain |
| US9420770B2 (en) | 2009-12-01 | 2016-08-23 | Indiana University Research & Technology Corporation | Methods of modulating thrombocytopenia and modified transgenic pigs |
| WO2011091134A2 (en) | 2010-01-22 | 2011-07-28 | Dana-Farber Cancer Institute, Inc. | Compositions,kits, and methods for identification, assessment, prevention, and therapy of metabolic disorders |
| TWI518325B (zh) | 2010-02-04 | 2016-01-21 | 自治醫科大學 | 對ａｌｋ抑制劑具有先抗性或後抗性癌症的識別、判斷和治療 |
| US10745467B2 (en) | 2010-03-26 | 2020-08-18 | The Trustees Of Dartmouth College | VISTA-Ig for treatment of autoimmune, allergic and inflammatory disorders |
| MX342017B (es) | 2010-03-26 | 2016-09-09 | Dartmouth College | Proteina mediadora de celula t regulatoria vista, agentes de enlace de vista y uso de los mismos. |
| US20150231215A1 (en) | 2012-06-22 | 2015-08-20 | Randolph J. Noelle | VISTA Antagonist and Methods of Use |
| US8383793B2 (en) | 2010-04-15 | 2013-02-26 | St. Jude Children's Research Hospital | Methods and compositions for the diagnosis and treatment of cancer resistant to anaplastic lymphoma kinase (ALK) kinase inhibitors |
| US20110269735A1 (en) | 2010-04-19 | 2011-11-03 | Celera Corporation | Genetic polymorphisms associated with statin response and cardiovascular diseases, methods of detection and uses thereof |
| WO2012051699A1 (en) | 2010-10-22 | 2012-04-26 | Agri-Neo Inc. | Synergistic activity of peracetic acid and at least one sar inducer for the control of pathogens in and onto growing plants |
| US20120108651A1 (en) | 2010-11-02 | 2012-05-03 | Leiden University Medical Center (LUMC) Acting on Behalf of Academic Hospital Leiden (AZL) | Genetic polymorphisms associated with venous thrombosis and statin response, methods of detection and uses thereof |
| WO2012064090A2 (ko) | 2010-11-08 | 2012-05-18 | 광주과학기술원 | 골격근-유래된 다능성 세포의 화학적 제조방법 및 이의 용도 |
| CA2827348C (en) | 2011-02-14 | 2021-02-23 | Revivicor, Inc. | Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof |
| WO2012129198A1 (en) | 2011-03-23 | 2012-09-27 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for obesity and diabetes |
| SI2709445T1 (sl) | 2011-05-16 | 2018-11-30 | The Curators Of The University Of Missouri | Živali, odporne proti virusu prašičjega reprodukcijskega in respiratornega sindroma |
| US10093705B2 (en) | 2011-09-13 | 2018-10-09 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for brown fat induction and activity using FNDC5 |
| LT2814842T (lt) | 2012-02-15 | 2018-11-12 | Novo Nordisk A/S | Antikūnai, kurie suriša peptidoglikaną atpažįstantį baltymą 1 |
| ES2640268T3 (es) | 2012-02-15 | 2017-11-02 | Novo Nordisk A/S | Anticuerpos que se unen a y bloquean un receptor desencadenante expresado en células mieloides 1 (TREM-1) |
| US9550830B2 (en) | 2012-02-15 | 2017-01-24 | Novo Nordisk A/S | Antibodies that bind and block triggering receptor expressed on myeloid cells-1 (TREM-1) |
| US9890215B2 (en) | 2012-06-22 | 2018-02-13 | King's College London | Vista modulators for diagnosis and treatment of cancer |
| CN104619722B (zh) | 2012-06-22 | 2022-10-04 | 达特茅斯大学理事会 | 新型vista-ig构建体和vista-ig用于治疗自身免疫性、过敏性和炎性疾病的用途 |
| CA2884704C (en) | 2012-09-07 | 2023-04-04 | Randolph J. Noelle | Vista modulators for diagnosis and treatment of cancer |
| EP4043493A1 (en) | 2013-12-24 | 2022-08-17 | Janssen Pharmaceutica NV | Anti-vista antibodies and fragments |
| US11014987B2 (en) | 2013-12-24 | 2021-05-25 | Janssen Pharmaceutics Nv | Anti-vista antibodies and fragments, uses thereof, and methods of identifying same |
| AU2015206603B9 (en) | 2014-01-14 | 2019-07-18 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for identification, assessment, prevention, and treatment of melanoma using PD-L1 isoforms |
| EP3154585B1 (en) | 2014-06-11 | 2022-02-23 | Kathy A. Green | Use of vista agonists and antagonists to suppress or enhance humoral immunity |
| KR102641268B1 (ko) | 2014-07-17 | 2024-02-27 | 노보 노르디스크 에이/에스 | 점도를 감소시키기 위한 trem-1 항체의 부위 지정 돌연변이유발 |
| US10077420B2 (en) | 2014-12-02 | 2018-09-18 | Histogenics Corporation | Cell and tissue culture container |
| JP2018505911A (ja) | 2014-12-05 | 2018-03-01 | イミュネクスト，インコーポレーテッド | 推定上のｖｉｓｔａ受容体としてのｖｓｉｇ８の同定と、ｖｉｓｔａ／ｖｓｉｇ８調節剤を産生するためのその使用 |
| EP3808775B1 (en) | 2014-12-09 | 2024-04-17 | Regeneron Pharmaceuticals, Inc. | Non-human animals having a humanized cluster of differentiation 274 gene |
| US10136642B2 (en) | 2015-02-19 | 2018-11-27 | Agri-Neo, Inc. | Composition of peracetic acid and at least one organic fungicide for the control and/or the treatment of diseases associated with the presence of pathogens, and method, use and kit involving said composition |
| EP3722314A1 (en) | 2015-06-24 | 2020-10-14 | Janssen Pharmaceutica NV | Anti-vista antibodies and fragments |
| RU2018107842A (ru) | 2015-08-06 | 2019-09-09 | Дзе Кьюрейторз Оф Дзе Юниверсити Оф Миссури | Устойчивые к патогенам животные, имеющие модифицированные гены cd163 |
| EP3368157B1 (en) | 2015-10-29 | 2022-06-29 | Dana-Farber Cancer Institute, Inc. | Methods for identification, assessment, prevention, and treatment of metabolic disorders using pm20d1 and n-lipidated amino acids |
| TW202216201A (zh) | 2016-02-12 | 2022-05-01 | 比利時商楊森製藥公司 | 抗vista抗體及片段、其用途及鑑定其之方法 |
| JP7184751B2 (ja) | 2016-04-15 | 2022-12-06 | イミュネクスト インコーポレイテッド | 抗ヒトｖｉｓｔａ抗体およびその使用 |
| AU2019248547B2 (en) | 2018-04-02 | 2025-07-17 | Bristol-Myers Squibb Company | Anti-TREM-1 antibodies and uses thereof |
| US11160260B2 (en) | 2018-04-17 | 2021-11-02 | The Curators Of The University Of Missouri | Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV) |
| CN117279502A (zh) | 2020-11-20 | 2023-12-22 | 雷维维科公司 | 用于异种移植的生长激素受体敲除的多转基因猪 |
| EP4404740A1 (en) | 2021-09-20 | 2024-07-31 | Revivicor Inc. | Multitransgenic pigs comprising ten genetic modifications for xenotransplantation |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ224241A (en) * | 1987-04-14 | 1991-03-26 | Bresatec Ltd | Method of producing new animal breeds using plasmid expression vectors; plasmid expression vectors |
| US5057420A (en) | 1987-06-05 | 1991-10-15 | Granada Biosciences, Inc. | Bovine nuclear transplantation |
| CA2059199A1 (en) | 1992-01-10 | 1993-07-11 | Steen Willadsen | Method for producing mature reconstituted mammalian eggs by nuclear transplantation |
| GB2265909B (en) | 1992-04-01 | 1996-03-20 | Inst Of Animal Physiology And | Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion |
| AU680441B2 (en) * | 1992-09-22 | 1997-07-31 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Use of taxol for treating lymphomas and breast cancer |
| CA2092258A1 (en) | 1992-12-30 | 1994-07-01 | Steven L. Stice | Synchronized donor cells for bovine nuclear transfer |
| US5480772A (en) | 1993-02-03 | 1996-01-02 | Brandeis University | In vitro activation of a nucleus |
| US5496720A (en) | 1993-02-10 | 1996-03-05 | Susko-Parrish; Joan L. | Parthenogenic oocyte activation |
| US5523226A (en) | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| US5333948A (en) | 1993-06-22 | 1994-08-02 | Tekonsha Engineering Company | Multiple-gain electronic brake actuator with trigger point inertial sensor |
| ES2255477T5 (es) * | 1993-07-29 | 2010-04-08 | The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Uso de paclitaxel y sus derivados en la fabricacion de un medicamento para el tratamiento de reestenosis. |
| AU1595195A (en) | 1993-12-23 | 1995-07-10 | Abs Global, Inc. | Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals |
| GB9417831D0 (en) * | 1994-09-05 | 1994-10-26 | Biotech & Biolog Scien Res | Biological manipulation |
| US7307198B2 (en) * | 1995-08-31 | 2007-12-11 | Roslin Institute | Ungulates produced by nuclear transfer of G1 cells |
| GB9517779D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| GB9517780D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| US6215041B1 (en) | 1997-01-10 | 2001-04-10 | University Of Mmassachusetts | Cloning using donor nuclei from a non-quiesecent somatic cells |
| US5945577A (en) | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| US7291764B1 (en) * | 1997-01-10 | 2007-11-06 | University of Massachusetts, a Public Institution of Higher Education of the Commonwealth of Massachusetts, as Represented by its Amherst Campus, Office of Vice Chancellor for Research at Amherst | Cloning pigs using non-quiescent differentiated donor cells or nuclei |
| US6235969B1 (en) | 1997-01-10 | 2001-05-22 | University Of Massachusetts | Cloning pigs using donor nuclei from non-quiescent differentiated cells |
| US7361804B1 (en) * | 1997-02-19 | 2008-04-22 | Roslin Institute (Edinburgh) | Unactivated oocytes in nuclear transfer to produce ungulates |
| US6011197A (en) | 1997-03-06 | 2000-01-04 | Infigen, Inc. | Method of cloning bovines using reprogrammed non-embryonic bovine cells |
| US6600087B1 (en) | 1998-01-16 | 2003-07-29 | Agrobiogen Gmbh | Efficient nuclear transfer using fetal fibroblasts and multiple cloning procedures |
| JP3573964B2 (ja) * | 1998-06-17 | 2004-10-06 | 三洋電機株式会社 | アルカリ電池用水素吸蔵合金電極及びアルカリ蓄電池用水素吸蔵合金電極の製造方法 |
| WO2000022098A1 (en) * | 1998-10-12 | 2000-04-20 | Geron Bio-Med Limited | Porcine oocytes with improved developmental competence |
| US6700037B2 (en) * | 1998-11-24 | 2004-03-02 | Infigen, Inc. | Method of cloning porcine animals |
| US20050156424A1 (en) * | 2000-02-29 | 2005-07-21 | Thyssenkrupp Presta Ag | Steering column and manufacturing method thereof |
| US8302202B2 (en) * | 2005-08-03 | 2012-10-30 | International Business Machines Corporation | Transportable computing environment apparatus system and method |
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