GB2265909B - Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion - Google Patents

Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion

Info

Publication number
GB2265909B
GB2265909B GB9207168A GB9207168A GB2265909B GB 2265909 B GB2265909 B GB 2265909B GB 9207168 A GB9207168 A GB 9207168A GB 9207168 A GB9207168 A GB 9207168A GB 2265909 B GB2265909 B GB 2265909B
Authority
GB
United Kingdom
Prior art keywords
fusion
absence
calcium ion
donor nucleus
recipient cytoplast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB9207168A
Other versions
GB2265909A (en
GB9207168D0 (en
Inventor
Fangzhen Sun
Robert Michael Moor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INST OF ANIMAL PHYSIOLOGY AND
Babraham Institute
Original Assignee
INST OF ANIMAL PHYSIOLOGY AND
Babraham Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INST OF ANIMAL PHYSIOLOGY AND, Babraham Institute filed Critical INST OF ANIMAL PHYSIOLOGY AND
Priority to GB9207168A priority Critical patent/GB2265909B/en
Publication of GB9207168D0 publication Critical patent/GB9207168D0/en
Publication of GB2265909A publication Critical patent/GB2265909A/en
Application granted granted Critical
Publication of GB2265909B publication Critical patent/GB2265909B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
GB9207168A 1992-04-01 1992-04-01 Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion Expired - Fee Related GB2265909B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9207168A GB2265909B (en) 1992-04-01 1992-04-01 Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9207168A GB2265909B (en) 1992-04-01 1992-04-01 Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion

Publications (3)

Publication Number Publication Date
GB9207168D0 GB9207168D0 (en) 1992-05-13
GB2265909A GB2265909A (en) 1993-10-13
GB2265909B true GB2265909B (en) 1996-03-20

Family

ID=10713280

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9207168A Expired - Fee Related GB2265909B (en) 1992-04-01 1992-04-01 Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion

Country Status (1)

Country Link
GB (1) GB2265909B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9517780D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB2318792B (en) * 1995-08-31 2000-06-21 Roslin Inst Unactivated oocytes as cytoplast recipients for nuclear transfer
GB9517779D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US7304204B2 (en) 1995-08-31 2007-12-04 Roslin Institute Ungulates produced by nuclear transfer of G1 cells
US7361804B1 (en) 1997-02-19 2008-04-22 Roslin Institute (Edinburgh) Unactivated oocytes in nuclear transfer to produce ungulates
CN102719444B (en) 2006-09-01 2016-12-14 人类多细胞株治疗学公司 The expression that people or Humanized immunoglobulin strengthen in non-human transgenic animal

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Cell Differentiation 1984, 14 (1), 47-52 *
Theriogenology 1990, 33(1), 301 *

Also Published As

Publication number Publication date
GB2265909A (en) 1993-10-13
GB9207168D0 (en) 1992-05-13

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20070401