ES2264165T3 - Deteccion especifica de metilacion. - Google Patents
Deteccion especifica de metilacion.Info
- Publication number
- ES2264165T3 ES2264165T3 ES97927933T ES97927933T ES2264165T3 ES 2264165 T3 ES2264165 T3 ES 2264165T3 ES 97927933 T ES97927933 T ES 97927933T ES 97927933 T ES97927933 T ES 97927933T ES 2264165 T3 ES2264165 T3 ES 2264165T3
- Authority
- ES
- Spain
- Prior art keywords
- baselineskip
- seq
- primers
- target sequence
- pair
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 80
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 60
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 58
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 58
- 238000006243 chemical reaction Methods 0.000 claims abstract description 31
- 239000013615 primer Substances 0.000 claims description 308
- 230000003321 amplification Effects 0.000 claims description 156
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 156
- 230000011987 methylation Effects 0.000 claims description 90
- 238000007069 methylation reaction Methods 0.000 claims description 90
- 210000004027 cell Anatomy 0.000 claims description 82
- 108090000623 proteins and genes Proteins 0.000 claims description 55
- 108010031372 Tissue Inhibitor of Metalloproteinase-2 Proteins 0.000 claims description 42
- 102100026262 Metalloproteinase inhibitor 2 Human genes 0.000 claims description 39
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 33
- 210000001519 tissue Anatomy 0.000 claims description 33
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical group OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims description 30
- 108091029430 CpG site Proteins 0.000 claims description 16
- 239000002253 acid Substances 0.000 claims description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims description 15
- 108091008146 restriction endonucleases Proteins 0.000 claims description 15
- -1 GST-pi Proteins 0.000 claims description 14
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 14
- 239000003795 chemical substances by application Substances 0.000 claims description 14
- 230000008569 process Effects 0.000 claims description 13
- 102000040430 polynucleotide Human genes 0.000 claims description 12
- 108091033319 polynucleotide Proteins 0.000 claims description 12
- 239000002157 polynucleotide Substances 0.000 claims description 12
- 239000003155 DNA primer Substances 0.000 claims description 11
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 11
- 230000004075 alteration Effects 0.000 claims description 11
- 208000029742 colonic neoplasm Diseases 0.000 claims description 11
- 239000002773 nucleotide Substances 0.000 claims description 11
- 125000003729 nucleotide group Chemical group 0.000 claims description 11
- 230000002062 proliferating effect Effects 0.000 claims description 11
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 9
- 108700025716 Tumor Suppressor Genes Proteins 0.000 claims description 9
- 102000044209 Tumor Suppressor Genes Human genes 0.000 claims description 9
- 229940104302 cytosine Drugs 0.000 claims description 9
- 201000005202 lung cancer Diseases 0.000 claims description 9
- 208000020816 lung neoplasm Diseases 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 8
- 108050007957 Cadherin Proteins 0.000 claims description 7
- 102000000905 Cadherin Human genes 0.000 claims description 7
- 101001134036 Homo sapiens DNA mismatch repair protein Msh2 Proteins 0.000 claims description 7
- 210000001072 colon Anatomy 0.000 claims description 7
- 206010009944 Colon cancer Diseases 0.000 claims description 6
- 102100034157 DNA mismatch repair protein Msh2 Human genes 0.000 claims description 6
- 206010036790 Productive cough Diseases 0.000 claims description 6
- 210000003802 sputum Anatomy 0.000 claims description 6
- 208000024794 sputum Diseases 0.000 claims description 6
- 102000055006 Calcitonin Human genes 0.000 claims description 5
- 108060001064 Calcitonin Proteins 0.000 claims description 5
- 102100028843 DNA mismatch repair protein Mlh1 Human genes 0.000 claims description 5
- 102100038595 Estrogen receptor Human genes 0.000 claims description 5
- 208000008839 Kidney Neoplasms Diseases 0.000 claims description 5
- 108010026664 MutL Protein Homolog 1 Proteins 0.000 claims description 5
- 210000000481 breast Anatomy 0.000 claims description 5
- BBBFJLBPOGFECG-VJVYQDLKSA-N calcitonin Chemical compound N([C@H](C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(N)=O)C(C)C)C(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1 BBBFJLBPOGFECG-VJVYQDLKSA-N 0.000 claims description 5
- 229960004015 calcitonin Drugs 0.000 claims description 5
- 108010038795 estrogen receptors Proteins 0.000 claims description 5
- 208000032839 leukemia Diseases 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 108020003175 receptors Proteins 0.000 claims description 5
- 229940035893 uracil Drugs 0.000 claims description 5
- 206010003571 Astrocytoma Diseases 0.000 claims description 4
- 102100037738 Fatty acid-binding protein, heart Human genes 0.000 claims description 4
- 101710136552 Fatty acid-binding protein, heart Proteins 0.000 claims description 4
- 102100031612 Hypermethylated in cancer 1 protein Human genes 0.000 claims description 4
- 101710133850 Hypermethylated in cancer 1 protein Proteins 0.000 claims description 4
- 206010038389 Renal cancer Diseases 0.000 claims description 4
- 206010006187 Breast cancer Diseases 0.000 claims description 3
- 208000026310 Breast neoplasm Diseases 0.000 claims description 3
- 102100039289 Glial fibrillary acidic protein Human genes 0.000 claims description 3
- 101710193519 Glial fibrillary acidic protein Proteins 0.000 claims description 3
- 229910015837 MSH2 Inorganic materials 0.000 claims description 3
- 206010060862 Prostate cancer Diseases 0.000 claims description 3
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 210000004556 brain Anatomy 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 claims description 3
- 210000005046 glial fibrillary acidic protein Anatomy 0.000 claims description 3
- 201000010982 kidney cancer Diseases 0.000 claims description 3
- 102000005962 receptors Human genes 0.000 claims description 3
- 210000003296 saliva Anatomy 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- 102000052609 BRCA2 Human genes 0.000 claims description 2
- 108700020462 BRCA2 Proteins 0.000 claims description 2
- 101150008921 Brca2 gene Proteins 0.000 claims description 2
- 206010014733 Endometrial cancer Diseases 0.000 claims description 2
- 206010014759 Endometrial neoplasm Diseases 0.000 claims description 2
- 101000954986 Homo sapiens Merlin Proteins 0.000 claims description 2
- 101000613251 Homo sapiens Tumor susceptibility gene 101 protein Proteins 0.000 claims description 2
- 208000000172 Medulloblastoma Diseases 0.000 claims description 2
- 102100037106 Merlin Human genes 0.000 claims description 2
- 206010029260 Neuroblastoma Diseases 0.000 claims description 2
- 102000007530 Neurofibromin 1 Human genes 0.000 claims description 2
- 108010085793 Neurofibromin 1 Proteins 0.000 claims description 2
- 102100022828 Retinoblastoma-like protein 2 Human genes 0.000 claims description 2
- 108050002651 Retinoblastoma-like protein 2 Proteins 0.000 claims description 2
- 102100040879 Tumor susceptibility gene 101 protein Human genes 0.000 claims description 2
- 206010002224 anaplastic astrocytoma Diseases 0.000 claims description 2
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims description 2
- 208000005017 glioblastoma Diseases 0.000 claims description 2
- 230000003394 haemopoietic effect Effects 0.000 claims description 2
- 230000002611 ovarian Effects 0.000 claims description 2
- 230000002685 pulmonary effect Effects 0.000 claims description 2
- 230000002381 testicular Effects 0.000 claims description 2
- 210000001541 thymus gland Anatomy 0.000 claims description 2
- 210000002700 urine Anatomy 0.000 claims description 2
- 102000002045 Endothelin Human genes 0.000 claims 1
- 108050009340 Endothelin Proteins 0.000 claims 1
- 239000003098 androgen Substances 0.000 claims 1
- ZUBDGKVDJUIMQQ-UBFCDGJISA-N endothelin-1 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(O)=O)NC(=O)[C@H]1NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@@H](CC=2C=CC(O)=CC=2)NC(=O)[C@H](C(C)C)NC(=O)[C@H]2CSSC[C@@H](C(N[C@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N2)=O)NC(=O)[C@@H](CO)NC(=O)[C@H](N)CSSC1)C1=CNC=N1 ZUBDGKVDJUIMQQ-UBFCDGJISA-N 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 9
- 108020004414 DNA Proteins 0.000 description 330
- 206010028980 Neoplasm Diseases 0.000 description 53
- 239000000047 product Substances 0.000 description 48
- 238000004458 analytical method Methods 0.000 description 35
- 239000000523 sample Substances 0.000 description 30
- 102100033254 Tumor suppressor ARF Human genes 0.000 description 29
- 108700028369 Alleles Proteins 0.000 description 28
- 238000012163 sequencing technique Methods 0.000 description 25
- 201000011510 cancer Diseases 0.000 description 24
- 108091029523 CpG island Proteins 0.000 description 19
- 102000004190 Enzymes Human genes 0.000 description 19
- 108090000790 Enzymes Proteins 0.000 description 19
- 229940088598 enzyme Drugs 0.000 description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 15
- 230000000295 complement effect Effects 0.000 description 14
- 230000006607 hypermethylation Effects 0.000 description 14
- 230000004048 modification Effects 0.000 description 14
- 238000012986 modification Methods 0.000 description 14
- 238000011282 treatment Methods 0.000 description 14
- 230000015572 biosynthetic process Effects 0.000 description 13
- 239000000203 mixture Substances 0.000 description 13
- 238000003786 synthesis reaction Methods 0.000 description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 238000009396 hybridization Methods 0.000 description 12
- 230000014509 gene expression Effects 0.000 description 11
- 230000002779 inactivation Effects 0.000 description 10
- 101800000504 3C-like protease Proteins 0.000 description 9
- 102100036464 Activated RNA polymerase II transcriptional coactivator p15 Human genes 0.000 description 9
- 230000000692 anti-sense effect Effects 0.000 description 9
- 239000000499 gel Substances 0.000 description 9
- 101800000607 p15 Proteins 0.000 description 9
- 210000001766 X chromosome Anatomy 0.000 description 8
- 238000003018 immunoassay Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 238000013518 transcription Methods 0.000 description 8
- 230000035897 transcription Effects 0.000 description 8
- 230000007067 DNA methylation Effects 0.000 description 7
- 108091034117 Oligonucleotide Proteins 0.000 description 7
- 229920001184 polypeptide Polymers 0.000 description 7
- 102000004196 processed proteins & peptides Human genes 0.000 description 7
- 108090000765 processed proteins & peptides Proteins 0.000 description 7
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 7
- 239000003981 vehicle Substances 0.000 description 7
- 101001081170 Homo sapiens Humanin-like 12 Proteins 0.000 description 6
- 102100027737 Humanin-like 12 Human genes 0.000 description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
- 238000012408 PCR amplification Methods 0.000 description 6
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 6
- 238000004925 denaturation Methods 0.000 description 6
- 230000036425 denaturation Effects 0.000 description 6
- 238000003745 diagnosis Methods 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 239000012188 paraffin wax Substances 0.000 description 6
- 229920002401 polyacrylamide Polymers 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 5
- 208000032818 Microsatellite Instability Diseases 0.000 description 5
- 230000001594 aberrant effect Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 238000007901 in situ hybridization Methods 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 5
- 238000011065 in-situ storage Methods 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- 108700042657 p16 Genes Proteins 0.000 description 5
- 238000006116 polymerization reaction Methods 0.000 description 5
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 108010017826 DNA Polymerase I Proteins 0.000 description 4
- 102000004594 DNA Polymerase I Human genes 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 238000002105 Southern blotting Methods 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 208000035475 disorder Diseases 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 210000004072 lung Anatomy 0.000 description 4
- 210000004698 lymphocyte Anatomy 0.000 description 4
- 230000001613 neoplastic effect Effects 0.000 description 4
- 239000012985 polymerization agent Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 208000005623 Carcinogenesis Diseases 0.000 description 3
- 102000013128 Endothelin B Receptor Human genes 0.000 description 3
- 108010090557 Endothelin B Receptor Proteins 0.000 description 3
- 108060004795 Methyltransferase Proteins 0.000 description 3
- 208000006265 Renal cell carcinoma Diseases 0.000 description 3
- 108010006785 Taq Polymerase Proteins 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 239000013060 biological fluid Substances 0.000 description 3
- 230000036952 cancer formation Effects 0.000 description 3
- 231100000504 carcinogenesis Toxicity 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 238000007385 chemical modification Methods 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 230000030279 gene silencing Effects 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 description 3
- 238000013507 mapping Methods 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 3
- 241000023308 Acca Species 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 2
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 2
- 238000004435 EPR spectroscopy Methods 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 101000645296 Homo sapiens Metalloproteinase inhibitor 2 Proteins 0.000 description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 2
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 102000001218 Rec A Recombinases Human genes 0.000 description 2
- 108010055016 Rec A Recombinases Proteins 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000008827 biological function Effects 0.000 description 2
- 230000031018 biological processes and functions Effects 0.000 description 2
- 239000012067 demethylated product Substances 0.000 description 2
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 2
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000001415 gene therapy Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 229940029575 guanosine Drugs 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000002595 magnetic resonance imaging Methods 0.000 description 2
- 230000036210 malignancy Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 238000007855 methylation-specific PCR Methods 0.000 description 2
- 239000002480 mineral oil Substances 0.000 description 2
- 235000010446 mineral oil Nutrition 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 210000002307 prostate Anatomy 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000007363 ring formation reaction Methods 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 239000001226 triphosphate Substances 0.000 description 2
- 235000011178 triphosphate Nutrition 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- BAAVRTJSLCSMNM-CMOCDZPBSA-N (2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]-4-carboxybutanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]pentanedioic acid Chemical compound C([C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CCC(O)=O)C(O)=O)C1=CC=C(O)C=C1 BAAVRTJSLCSMNM-CMOCDZPBSA-N 0.000 description 1
- XDQFBMPFEBUDIC-UHFFFAOYSA-N 1h-pyrimidine-2,4-dione Chemical compound O=C1C=CNC(=O)N1.O=C1C=CNC(=O)N1 XDQFBMPFEBUDIC-UHFFFAOYSA-N 0.000 description 1
- GPLIMIJPIZGPIF-UHFFFAOYSA-N 2-hydroxy-1,4-benzoquinone Chemical compound OC1=CC(=O)C=CC1=O GPLIMIJPIZGPIF-UHFFFAOYSA-N 0.000 description 1
- 108020005065 3' Flanking Region Proteins 0.000 description 1
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 108020005029 5' Flanking Region Proteins 0.000 description 1
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102100032187 Androgen receptor Human genes 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 208000003174 Brain Neoplasms Diseases 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 1
- 208000030808 Clear cell renal carcinoma Diseases 0.000 description 1
- 108091033380 Coding strand Proteins 0.000 description 1
- 108050006400 Cyclin Proteins 0.000 description 1
- 230000008836 DNA modification Effects 0.000 description 1
- 230000007018 DNA scission Effects 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 208000032612 Glial tumor Diseases 0.000 description 1
- 206010018338 Glioma Diseases 0.000 description 1
- 102000005741 Metalloproteases Human genes 0.000 description 1
- 108010006035 Metalloproteases Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 102100036691 Proliferating cell nuclear antigen Human genes 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 208000007660 Residual Neoplasm Diseases 0.000 description 1
- 201000000582 Retinoblastoma Diseases 0.000 description 1
- 108700025701 Retinoblastoma Genes Proteins 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- MEFKEPWMEQBLKI-AIRLBKTGSA-N S-adenosyl-L-methioninate Chemical compound O[C@@H]1[C@H](O)[C@@H](C[S+](CC[C@H](N)C([O-])=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MEFKEPWMEQBLKI-AIRLBKTGSA-N 0.000 description 1
- 102220497176 Small vasohibin-binding protein_T47D_mutation Human genes 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 108700025695 Suppressor Genes Proteins 0.000 description 1
- 102000005354 Tissue Inhibitor of Metalloproteinase-2 Human genes 0.000 description 1
- 102000005876 Tissue Inhibitor of Metalloproteinases Human genes 0.000 description 1
- 108010005246 Tissue Inhibitor of Metalloproteinases Proteins 0.000 description 1
- 108010040002 Tumor Suppressor Proteins Proteins 0.000 description 1
- 102000001742 Tumor Suppressor Proteins Human genes 0.000 description 1
- 101150046474 Vhl gene Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 108700031765 Von Hippel-Lindau Tumor Suppressor Proteins 0.000 description 1
- SWPYNTWPIAZGLT-UHFFFAOYSA-N [amino(ethoxy)phosphanyl]oxyethane Chemical class CCOP(N)OCC SWPYNTWPIAZGLT-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 229960001570 ademetionine Drugs 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- 108010080146 androgen receptors Proteins 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 108010058966 bacteriophage T7 induced DNA polymerase Proteins 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000010504 bond cleavage reaction Methods 0.000 description 1
- 208000035269 cancer or benign tumor Diseases 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000007806 chemical reaction intermediate Substances 0.000 description 1
- 230000002113 chemopreventative effect Effects 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000001246 colloidal dispersion Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000002967 competitive immunoassay Methods 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 230000009615 deamination Effects 0.000 description 1
- 238000006481 deamination reaction Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 239000005549 deoxyribonucleoside Substances 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 238000002059 diagnostic imaging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 230000014200 hypermethylation of CpG island Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000013198 immunometric assay Methods 0.000 description 1
- 238000007850 in situ PCR Methods 0.000 description 1
- 238000011503 in vivo imaging Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 229940043355 kinase inhibitor Drugs 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000005291 magnetic effect Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000003475 metalloproteinase inhibitor Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 230000033607 mismatch repair Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000036963 noncompetitive effect Effects 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 239000003757 phosphotransferase inhibitor Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000014493 regulation of gene expression Effects 0.000 description 1
- 201000010174 renal carcinoma Diseases 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 125000001273 sulfonato group Chemical group [O-]S(*)(=O)=O 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000011285 therapeutic regimen Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- 108010032276 tyrosyl-glutamyl-tyrosyl-glutamic acid Proteins 0.000 description 1
- 230000009452 underexpressoin Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
- Photoreceptors In Electrophotography (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/656,716 US5786146A (en) | 1996-06-03 | 1996-06-03 | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US656716 | 1996-06-03 | ||
| US08/835,728 US6017704A (en) | 1996-06-03 | 1997-04-11 | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US835728 | 1997-04-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2264165T3 true ES2264165T3 (es) | 2006-12-16 |
Family
ID=27097252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES97927933T Expired - Lifetime ES2264165T3 (es) | 1996-06-03 | 1997-06-03 | Deteccion especifica de metilacion. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US6017704A (enExample) |
| EP (2) | EP1690948A3 (enExample) |
| JP (2) | JP3612080B2 (enExample) |
| AT (1) | ATE326549T1 (enExample) |
| CA (1) | CA2257104C (enExample) |
| DE (1) | DE69735894T2 (enExample) |
| DK (1) | DK0954608T3 (enExample) |
| ES (1) | ES2264165T3 (enExample) |
| IL (1) | IL127342A (enExample) |
| PT (1) | PT954608E (enExample) |
| WO (1) | WO1997046705A1 (enExample) |
Families Citing this family (310)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5871917A (en) | 1996-05-31 | 1999-02-16 | North Shore University Hospital Research Corp. | Identification of differentially methylated and mutated nucleic acids |
| US6617434B1 (en) | 1996-05-31 | 2003-09-09 | North Shore Long Island Jewish Research Institute | Identificiaton of differentially methylated and mutated nucleic acids |
| US5786146A (en) * | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| WO1998056952A1 (en) * | 1997-06-09 | 1998-12-17 | University Of Southern California | A cancer diagnostic method based upon dna methylation differences |
| US6818404B2 (en) | 1997-10-23 | 2004-11-16 | Exact Sciences Corporation | Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples |
| DE19754482A1 (de) * | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| AUPP312998A0 (en) | 1998-04-23 | 1998-05-14 | Commonwealth Scientific And Industrial Research Organisation | Diagnostic assay |
| US7700324B1 (en) * | 1998-11-03 | 2010-04-20 | The Johns Hopkins University School Of Medicine | Methylated CpG island amplification (MCA) |
| US6994991B1 (en) | 1998-11-18 | 2006-02-07 | North Shore - Long Island Jewish Research Institute | Identification of differentially methylated multiple drug resistance loci |
| DE19853398C1 (de) * | 1998-11-19 | 2000-03-16 | Epigenomics Gmbh | Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischer DNA |
| DE19905082C1 (de) * | 1999-01-29 | 2000-05-18 | Epigenomics Gmbh | Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben |
| AUPP844899A0 (en) * | 1999-02-01 | 1999-02-25 | University Of Western Australia, The | A method for detecting methylated nucleic acids |
| US7655443B1 (en) * | 1999-05-07 | 2010-02-02 | Siemens Healthcare Diagnostics, Inc. | Nucleic acid sequencing with simultaneous quantitation |
| US6331393B1 (en) * | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| US6709818B1 (en) * | 1999-10-13 | 2004-03-23 | The Johns Hopkins University School Of Medicine | Methods of diagnosing and treating hepatic cell proliferative disorders |
| US20030207297A1 (en) * | 1999-10-13 | 2003-11-06 | Hubert Koster | Methods for generating databases and databases for identifying polymorphic genetic markers |
| WO2001026536A2 (en) * | 1999-10-13 | 2001-04-19 | The Johns Hopkins University School Of Medicine | Methods of diagnosing and treating hepatic cell proliferative disorders |
| US7668658B2 (en) * | 1999-10-13 | 2010-02-23 | Sequenom, Inc. | Methods for generating databases and databases for identifying polymorphic genetic markers |
| EP1228247B1 (de) * | 1999-11-12 | 2004-08-04 | Epigenomics AG | Verfahren zur kontrollierbaren durchführung komplexer pcr-amplifikationen |
| DE19957827C2 (de) * | 1999-11-25 | 2003-06-12 | Epigenomics Ag | Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche |
| US20030170649A1 (en) * | 1999-12-03 | 2003-09-11 | Haas Oskar A. | Method for detecting and evaluating a potentially aberrantly methylated dna region on the x chromosome, or the clonality |
| DE19959691A1 (de) * | 1999-12-06 | 2001-08-16 | Epigenomics Ag | Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA |
| GB9929720D0 (en) * | 1999-12-17 | 2000-02-09 | Zeneca Ltd | Diagnostic method |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US8076063B2 (en) | 2000-02-07 | 2011-12-13 | Illumina, Inc. | Multiplexed methylation detection methods |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
| DE10010282B4 (de) | 2000-02-25 | 2006-11-16 | Epigenomics Ag | Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| DE10013847A1 (de) * | 2000-03-15 | 2001-09-27 | Epigenomics Ag | Oligonukleotide oder PNA-Oligomere und Verfahren zur parallelen Detektion des Methylierungszustandes genomischer DNA |
| WO2001077376A2 (en) | 2000-04-06 | 2001-10-18 | Epigenomics Ag | Diagnosis of diseases associated with metastasis |
| US20040006036A1 (en) * | 2000-04-12 | 2004-01-08 | Gmr, A Delaware Corporation | Silencing transcription by methylation |
| DE10029915B4 (de) * | 2000-06-19 | 2005-06-16 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| AU2001277521A1 (en) * | 2000-06-30 | 2002-01-14 | Epigenomics Ag | Diagnosis of diseases associated with cell signalling |
| CA2312051A1 (en) | 2000-07-13 | 2002-01-13 | Quebepharma Inc. | Methylated nucleotide regulation of gene expression |
| DE10038733A1 (de) * | 2000-08-02 | 2002-02-21 | Epigenomics Ag | Verfahren zur Altersbestimmung von Individuen |
| DE60136212D1 (de) * | 2000-08-15 | 2008-11-27 | Univ Johns Hopkins Med | Diagnose und behandlung von tumor-suppressor assoziierten krankheiten |
| EP1352089B1 (en) * | 2000-08-25 | 2006-12-06 | Lovelace Respiratory Research Institute | Nested methylation-specific polymerase chain reaction cancer detection method |
| WO2002061410A1 (en) * | 2000-09-01 | 2002-08-08 | Spectrumedix Corporation | System and method for temperature gradient capillary electrophoresis |
| US6773897B2 (en) * | 2000-09-29 | 2004-08-10 | The Johns Hopkins University School Of Medicine | Method of predicting the clinical response to chemotherapeutic treatment with alkylating agents |
| DE10050942B4 (de) * | 2000-10-10 | 2005-11-17 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| WO2002031199A1 (en) * | 2000-10-11 | 2002-04-18 | Spectrumedix Corporation | System and method for determining the presence of methylated cytosines in polynucleotides |
| GB0025913D0 (en) | 2000-10-23 | 2000-12-06 | Guldberg Per | Materials and methods relating to nucleic acid amplification and profiling |
| WO2002034942A2 (en) * | 2000-10-23 | 2002-05-02 | Cancer Research Technology Limited | Nucleic acid amplification-based methods for the determination of a methylation profile and reagents therefor |
| EP1379685A2 (en) * | 2000-11-08 | 2004-01-14 | University Of Southern California | Assay for the detection and quantitation of hemimethylation |
| DE10056802B4 (de) * | 2000-11-14 | 2005-06-16 | Epigenomics Ag | Verfahren zur Detektion von Methylierungszuständen zur toxikologischen Diagnostik |
| US6756200B2 (en) | 2001-01-26 | 2004-06-29 | The Johns Hopkins University School Of Medicine | Aberrantly methylated genes as markers of breast malignancy |
| US6893820B1 (en) * | 2001-01-31 | 2005-05-17 | The Ohio State University Research Foundation | Detection of methylated CpG rich sequences diagnostic for malignant cells |
| EP1402071A4 (en) * | 2001-06-08 | 2005-12-14 | Us Genomics Inc | METHOD AND PRODUCTS FOR THE ANALYSIS OF NUCLEIC ACIDS BASED ON THE METHYLATION STATUS |
| DE10128508A1 (de) * | 2001-06-14 | 2003-02-06 | Epigenomics Ag | Verfahren und Nukleinsäuren für die Differenzierung von Prostata-Tumoren |
| DE10128509A1 (de) * | 2001-06-14 | 2003-01-02 | Epigenomics Ag | Verfahren und Nukleinsäuren für die Differenzierung von Prostata- und Nierenkarzinomen |
| WO2003012051A2 (en) * | 2001-07-31 | 2003-02-13 | The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Inhibitor of dna methylation |
| DE10139283A1 (de) * | 2001-08-09 | 2003-03-13 | Epigenomics Ag | Verfahren und Nukleinsäuren zur Analyse von Colon-Krebs |
| US6927028B2 (en) | 2001-08-31 | 2005-08-09 | Chinese University Of Hong Kong | Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA |
| US7432050B2 (en) * | 2001-10-05 | 2008-10-07 | Case Western Reserve University | Methods and compositions for detecting colon cancers |
| DE10151055B4 (de) * | 2001-10-05 | 2005-05-25 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln |
| WO2003064682A1 (en) * | 2001-10-18 | 2003-08-07 | Lovelace Respiratory Research Institute | Cancer monitoring by aberrant promotor methylation of the transcription factor genes pax5 alpha pax5 beta, novel loop helix loop protein, novel gene 2, and beta3 genes |
| EP1451372A1 (en) * | 2001-10-18 | 2004-09-01 | Spectrumedix Corporation | System and method for temperature gradient capillary electrophoresis |
| US20030099997A1 (en) * | 2001-10-24 | 2003-05-29 | Bestor Timothy H. | Method for gene identification based on differential DNA methylation |
| DE10154317B4 (de) | 2001-10-26 | 2005-06-09 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben |
| JP4381142B2 (ja) * | 2001-11-16 | 2009-12-09 | ザ ジョーンズ ホプキンス ユニバーシティー スクール オブ メディシン | 前立腺癌の検出法 |
| US20110151438A9 (en) | 2001-11-19 | 2011-06-23 | Affymetrix, Inc. | Methods of Analysis of Methylation |
| EP3098231A1 (en) | 2001-11-30 | 2016-11-30 | The John Hopkins University | Methods for analyzing methylated cpg islands and gc rich regions |
| DE10201138B4 (de) * | 2002-01-08 | 2005-03-10 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA) |
| AU2003212878A1 (en) * | 2002-01-30 | 2003-09-02 | Epigenomics Ag | Identification of cell differentiation states based on methylation patterns |
| US7776524B2 (en) * | 2002-02-15 | 2010-08-17 | Genzyme Corporation | Methods for analysis of molecular events |
| KR100470221B1 (ko) * | 2002-02-20 | 2005-02-05 | 굿젠 주식회사 | 유전자 프로모터 ＣｐＧ 아일랜드의 메틸화 여부를분석하는 염기서열분석형 올리고뉴클레오타이드칩, 이의제조방법 및 이를 이용한 암 검색방법 |
| EP1340818A1 (en) | 2002-02-27 | 2003-09-03 | Epigenomics AG | Method and nucleic acids for the analysis of a colon cell proliferative disorder |
| ATE312946T1 (de) * | 2002-03-05 | 2005-12-15 | Epigenomics Ag | Verfahren und vorrichtung zur bestimmung der gewebespezifität von freier dna in körperflüssigkeiten |
| AU2003230619A1 (en) * | 2002-03-08 | 2003-09-22 | Naxcor | Compositions and methods for detecting nucleic acid methylation |
| WO2003083107A1 (en) * | 2002-03-25 | 2003-10-09 | The Board Of Trustees Of The University Of Arkansas | Cpg retrieval of dna from formalin-fixed pathology specimen for promoter methylation analysis |
| US6872530B2 (en) * | 2002-04-24 | 2005-03-29 | Spectrumedix, Llc | Method for determining the presence of DNA variants using peptide nucleic acid probes |
| CA2484676A1 (en) * | 2002-05-03 | 2003-11-13 | Sequenom, Inc. | Kinase anchor protein muteins, peptides thereof, and related methods |
| WO2003104427A2 (en) * | 2002-06-05 | 2003-12-18 | Case Western Reserve University | Methods and compositions for detecting cancers |
| CN100427863C (zh) * | 2002-06-21 | 2008-10-22 | 有限会社日乃出 | 伞的沥水装置 |
| EP1534865A4 (en) * | 2002-06-26 | 2005-12-21 | Cold Spring Harbor Lab | METHOD AND COMPOSITIONS FOR DETERMINING METHYLATION PROFILES |
| US20040178070A1 (en) * | 2002-07-16 | 2004-09-16 | Zhaowei Liu | Method and system for comparative genomics for organisms using temperature gradient electrophoresis |
| ES2447566T3 (es) | 2002-10-01 | 2014-03-12 | Epigenomics Ag | Uso de ácidos nucleicos de PITX2 para mejorar el tratamiento de trastornos proliferativos de células mamarias |
| WO2004031402A2 (en) * | 2002-10-02 | 2004-04-15 | Northwestern University | Methylation profile of cancer |
| CA2442438C (en) | 2002-10-04 | 2012-06-19 | Nisshinbo Industries, Inc. | Oligonucleotide-immobilized substrate for detecting methylation |
| WO2004050839A2 (en) | 2002-11-27 | 2004-06-17 | Sequenom, Inc. | Fragmentation-based methods and systems for sequence variation detection and discovery |
| US20060094016A1 (en) * | 2002-12-02 | 2006-05-04 | Niall Gormley | Determination of methylation of nucleic acid sequences |
| US20040146868A1 (en) * | 2003-01-24 | 2004-07-29 | Epigenomics Ag | Methods and nucleic acids for the analysis of CpG dinucleotide methylation status associated with the development of peripheral zone prostate cancer |
| AU2003900368A0 (en) * | 2003-01-24 | 2003-02-13 | Human Genetic Signatures Pty Ltd | Assay for nucleic acid molecules |
| DE10304219B3 (de) * | 2003-01-30 | 2004-08-19 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
| US20070141570A1 (en) * | 2003-03-07 | 2007-06-21 | Sequenom, Inc. | Association of polymorphic kinase anchor proteins with cardiac phenotypes and related methods |
| JP2007524369A (ja) * | 2003-03-17 | 2007-08-30 | ザ ジョンズ ホプキンス ユニバーシティー | 膵癌における異常メチル化遺伝子 |
| WO2004087957A2 (en) * | 2003-04-03 | 2004-10-14 | Oncomethylome Sciences S.A. | Hypermethylated genes and cervical cancer |
| US20040203004A1 (en) * | 2003-04-10 | 2004-10-14 | Bernard Hans Ulrich | Diagnostic apparatus and method |
| WO2004097369A2 (en) * | 2003-04-25 | 2004-11-11 | Sequenom, Inc. | Fragmentation-based methods and systems for de novo sequencing |
| US7288373B2 (en) * | 2003-05-02 | 2007-10-30 | Human Genetic Signatures Pty Ltd. | Treatment of methylated nucleic acid |
| US20050009059A1 (en) * | 2003-05-07 | 2005-01-13 | Affymetrix, Inc. | Analysis of methylation status using oligonucleotide arrays |
| US20050026183A1 (en) * | 2003-05-15 | 2005-02-03 | Jian-Bing Fan | Methods and compositions for diagnosing conditions associated with specific DNA methylation patterns |
| US8150627B2 (en) | 2003-05-15 | 2012-04-03 | Illumina, Inc. | Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns |
| US8150626B2 (en) * | 2003-05-15 | 2012-04-03 | Illumina, Inc. | Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns |
| EP1641936B1 (en) * | 2003-06-17 | 2010-08-04 | Human Genetic Signatures PTY Ltd. | Methods for genome amplification |
| US20080064029A1 (en) * | 2003-06-23 | 2008-03-13 | Epigenomics Ag | Methods and Nucleic Acids for Analyses of Colorectal Cell Proliferative Disorders |
| EP2354249A1 (en) | 2003-06-23 | 2011-08-10 | Epigenomics AG | Methods and nucleic acids for analyses of colorectal cell proliferative disorders |
| US20040265833A1 (en) * | 2003-06-23 | 2004-12-30 | Cathy Lofton-Day | Methods and nucleic acids for the analysis of colorectal cell proliferative disorders |
| WO2005001140A2 (en) | 2003-06-23 | 2005-01-06 | Epigenomics Ag | Methods and nucleic acids for the analysis of colon cell proliferative disorders |
| US20070117093A1 (en) * | 2003-06-24 | 2007-05-24 | Reimo Tetzner | Heavymethyl assay for the methylation analysis of the gstpi gene |
| US7303879B2 (en) * | 2003-07-31 | 2007-12-04 | Applera Corporation | Determination of SNP allelic frequencies using temperature gradient electrophoresis |
| US20050123944A1 (en) * | 2003-08-01 | 2005-06-09 | U.S. Genomics, Inc. | Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions |
| US20060183128A1 (en) * | 2003-08-12 | 2006-08-17 | Epigenomics Ag | Methods and compositions for differentiating tissues for cell types using epigenetic markers |
| JP4781267B2 (ja) * | 2003-08-14 | 2011-09-28 | ケース ウエスタン リザーブ ユニバーシティ | 大腸癌を検出する方法及び組成物 |
| US8415100B2 (en) | 2003-08-14 | 2013-04-09 | Case Western Reserve University | Methods and compositions for detecting gastrointestinal and other cancers |
| DE10338308B4 (de) | 2003-08-15 | 2006-10-19 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in DNA |
| US7198900B2 (en) | 2003-08-29 | 2007-04-03 | Applera Corporation | Multiplex detection compositions, methods, and kits |
| CN1845991A (zh) * | 2003-08-29 | 2006-10-11 | 田中纪章 | 核酸扩增用引物以及利用该引物检查结肠癌的检查方法 |
| JP4714148B2 (ja) * | 2003-09-04 | 2011-06-29 | ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド | 核酸検出アッセイ |
| US9394565B2 (en) * | 2003-09-05 | 2016-07-19 | Agena Bioscience, Inc. | Allele-specific sequence variation analysis |
| DE10348407A1 (de) * | 2003-10-17 | 2005-05-19 | Widschwendter, Martin, Prof. | Prognostische und diagnostische Marker für Zell-proliferative Erkrankungen von Brustgeweben |
| ATE550438T1 (de) * | 2003-10-21 | 2012-04-15 | Orion Genomics Llc | Verfahren zur quantitativen bestimmung der methylierungsdichte in einem dna-locus |
| AU2004295712B2 (en) | 2003-12-01 | 2011-05-19 | Epigenomics Ag | Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders |
| DE602004031405D1 (de) | 2003-12-11 | 2011-03-31 | Epigenomics Ag | Marker zur Prognose der Reaktion auf Therapie bzw. der Überlebensrate von Brustkrebspatienten |
| DE102004002257B4 (de) * | 2004-01-09 | 2006-11-09 | Epigenomics Ag | Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA mit Hilfe von DNA-Reparaturenzymen |
| EP1718766A4 (en) * | 2004-02-10 | 2009-05-27 | Cornell Res Foundation Inc | PROCEDURE FOR DETECTING THE PROMOTER METHYLATION STATUS |
| WO2005085477A1 (en) * | 2004-03-02 | 2005-09-15 | Orion Genomics Llc | Differential enzymatic fragmentation by whole genome amplification |
| US7608394B2 (en) | 2004-03-26 | 2009-10-27 | Sequenom, Inc. | Methods and compositions for phenotype identification based on nucleic acid methylation |
| EP1727911B1 (en) * | 2004-03-26 | 2013-01-23 | Sequenom, Inc. | Base specific cleavage of methylation-specific amplification products in combination with mass analysis |
| US8168777B2 (en) | 2004-04-29 | 2012-05-01 | Human Genetic Signatures Pty. Ltd. | Bisulphite reagent treatment of nucleic acid |
| US20080241827A1 (en) * | 2004-05-10 | 2008-10-02 | Exact Sciences Corporation | Methods For Detecting A Mutant Nucleic Acid |
| WO2005118872A2 (en) * | 2004-06-01 | 2005-12-15 | The Regents Of The University Of Michigan | Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory diseases |
| US7943308B2 (en) * | 2004-06-23 | 2011-05-17 | Epigenomics Ag | Methods and nucleic acids for the detection of metastasis of colon cell proliferative disorders |
| WO2006008128A2 (en) | 2004-07-18 | 2006-01-26 | Epigenomics Ag | Epigenetic methods and nucleic acids for the detection of breast cell proliferative disorders |
| US7981607B2 (en) | 2004-08-27 | 2011-07-19 | Esoterix Genetic Laboratories LLC | Method for detecting recombinant event |
| WO2006026828A1 (en) * | 2004-09-10 | 2006-03-16 | Human Genetic Signatures Pty Ltd | Amplification blocker comprising intercalating nucleic acids (tna) containing intercalating pseudonucleotides (ipn) |
| CN101072882A (zh) * | 2004-09-10 | 2007-11-14 | 塞昆纳姆股份有限公司 | 用于核酸长程序列分析的方法 |
| KR100617649B1 (ko) | 2004-09-24 | 2006-09-04 | (주)지노믹트리 | 대장암 특이적 발현감소 유전자의 메틸화된 프로모터를 함유하는 암 진단용 조성물 및 그 용도 |
| US20080171318A1 (en) * | 2004-09-30 | 2008-07-17 | Epigenomics Ag | Epigenetic Methods and Nucleic Acids for the Detection of Lung Cell Proliferative Disorders |
| WO2006047787A2 (en) | 2004-10-27 | 2006-05-04 | Exact Sciences Corporation | Method for monitoring disease progression or recurrence |
| WO2006052976A2 (en) * | 2004-11-09 | 2006-05-18 | Schering Corporation | Improved dosing regimen of temozolomide for treating cancer based on the patient’s mgmt level |
| EP2213749A1 (en) | 2004-12-02 | 2010-08-04 | Epigenomics AG | Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders |
| US20060122162A1 (en) * | 2004-12-02 | 2006-06-08 | Schering Corporation | Methods of using temozolomide formulation intrathecally in the treatment of cancers |
| KR101293713B1 (ko) * | 2004-12-03 | 2013-08-12 | 휴먼 제네틱 시그너처스 피티와이 엘티디 | 시토신의 화학적 변형에 의한 미생물 핵산의 단순화 방법 |
| US20080118926A1 (en) * | 2004-12-13 | 2008-05-22 | National University Corporation Okayama University | Method For Detecting Methylation In Genes And Method For Examining Neoplasm Through Detecting Methylation In Genes |
| US20060134650A1 (en) * | 2004-12-21 | 2006-06-22 | Illumina, Inc. | Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis |
| CA2592078A1 (en) * | 2004-12-23 | 2006-06-29 | Human Genetic Signatures Pty Ltd | Detection of human papilloma virus |
| EP2272984B1 (en) | 2005-02-01 | 2012-06-20 | John Wayne Cancer Institute | Use of ID4 for diagnosis and treatment of cancer |
| WO2006088940A2 (en) | 2005-02-14 | 2006-08-24 | The Johns Hopkins University | Neoplasia screening compositions and methods of use |
| WO2006094149A2 (en) * | 2005-03-01 | 2006-09-08 | Exact Sciences Corporation | Methods and compositions for detecting adenoma |
| KR100735045B1 (ko) | 2005-04-07 | 2007-07-03 | (주) 차바이오텍 | 마우스의 ｂ1 서열을 이용한 ｄｎａ 메틸화를 측정하는방법 및 이를 이용한 항암제의 항암활성 측정법 |
| KR101708544B1 (ko) | 2005-04-15 | 2017-02-20 | 에피제노믹스 아게 | 세포 증식 질환을 분석하기 위한 방법 및 핵산 |
| WO2007044071A2 (en) * | 2005-04-21 | 2007-04-19 | Exact Sciences Corporation | Analysis of heterogeneous nucleic acid samples |
| EP2345743A1 (en) | 2005-05-02 | 2011-07-20 | University of Southern California | DNA methylation markers associated with the CpG island methylator phenotyp (CIMP) in human colorectal cancer |
| AU2006251866B2 (en) * | 2005-05-26 | 2007-11-29 | Human Genetic Signatures Pty Ltd | Isothermal strand displacement amplification using primers containing a non-regular base |
| US7439024B2 (en) * | 2005-06-01 | 2008-10-21 | The United States Of America As Represented By The Department Of Veterans Affairs | Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory diseases |
| US20070037184A1 (en) * | 2005-06-16 | 2007-02-15 | Applera Corporation | Methods and kits for evaluating dna methylation |
| US20060286577A1 (en) * | 2005-06-17 | 2006-12-21 | Xiyu Jia | Methods for detection of methylated DNA |
| US20070087360A1 (en) * | 2005-06-20 | 2007-04-19 | Boyd Victoria L | Methods and compositions for detecting nucleotides |
| US20060292585A1 (en) * | 2005-06-24 | 2006-12-28 | Affymetrix, Inc. | Analysis of methylation using nucleic acid arrays |
| US20090270479A1 (en) * | 2005-07-12 | 2009-10-29 | Antonio Giordano | Genetic and Epigenetic Alterations In the Diagnosis and Treatment of Cancer |
| US20090005268A1 (en) * | 2005-07-18 | 2009-01-01 | Epigenomics Ag | Compositions and Methods for Cancer Diagnostics Comprising Pan-Cancer Markers |
| US8343738B2 (en) * | 2005-09-14 | 2013-01-01 | Human Genetic Signatures Pty. Ltd. | Assay for screening for potential cervical cancer |
| US20070059753A1 (en) * | 2005-09-15 | 2007-03-15 | Tatiana Vener | Detecting gene methylation |
| WO2007039128A1 (en) * | 2005-09-21 | 2007-04-12 | Epigenomics Ag | Markers for the prediction of outcome of anthracycline treatment |
| EP2298932A1 (en) | 2005-09-29 | 2011-03-23 | Epigenomics AG | Methods and nucleic acids for the analysis of gene expression, in particular methylation of KAAG1, associated with tissue classification |
| US20080254470A1 (en) * | 2005-10-03 | 2008-10-16 | Epigenomics Ag | Methods and Nucleic Acids For the Analysis of Gene Expression Associated With the Prognosis of Cell Proliferative Disorders |
| WO2007044780A2 (en) * | 2005-10-07 | 2007-04-19 | Emory University | Methods and systems for screening for and diagnosing dna methylation associated abnormalities and sex chromosome aneuploidies |
| US7770717B2 (en) * | 2005-10-12 | 2010-08-10 | Scanvaegt International A/S | Device for transfer of items |
| US20070087358A1 (en) * | 2005-10-19 | 2007-04-19 | Melanie Ehrlich | Methods for diagnosing cancer based on DNA methylation status in NBL2 |
| WO2007102891A2 (en) * | 2005-11-08 | 2007-09-13 | Euclid Diagnostics Llc | Materials and methods for assaying for methylation of cpg islands associated with genes in the evaluation of cancer |
| WO2007057231A1 (en) * | 2005-11-17 | 2007-05-24 | Epigenomics Ag | Method for the determination of the dna methylation level of a cpg position in identical cells within a tissue sample |
| EP1973569B1 (en) | 2006-01-18 | 2013-05-22 | Merck Patent GmbH | Specific therapy using integrin ligands for treating cancer |
| US7820385B2 (en) * | 2006-03-22 | 2010-10-26 | The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention | Method for retaining methylation pattern in globally amplified DNA |
| EP1840224A1 (en) | 2006-03-29 | 2007-10-03 | Pangaea Biotech, S.A. | Method of predicting survival of a non-small-cell lung cancer patient to a chemotherapeutic treatment |
| WO2007123761A2 (en) * | 2006-03-29 | 2007-11-01 | John Wayne Cancer Institute | Methylation of estrogen receptor alpha and uses thereof |
| US7901882B2 (en) * | 2006-03-31 | 2011-03-08 | Affymetrix, Inc. | Analysis of methylation using nucleic acid arrays |
| US20090317810A1 (en) * | 2006-04-17 | 2009-12-24 | Epigenomics Ag | Methods and nucleic acids for the detection of colorectal cell proliferative disorders |
| AU2007237444B2 (en) | 2006-04-17 | 2013-05-23 | Epigenomics Ag | Methods and nucleic acids for the detection of colorectal cell proliferative disorders |
| US20070264672A1 (en) * | 2006-05-09 | 2007-11-15 | Bimalendu Dasmahapatra | Development of a novel assay for mgmt (methyl guanine methyl transferase) |
| US20080003609A1 (en) * | 2006-05-10 | 2008-01-03 | The Cleveland Clinic Foundation | Method of detecting bladder urothelial carcinoma |
| US20080081333A1 (en) * | 2006-05-26 | 2008-04-03 | University Of Maryland, Baltimore | Methylated promoters as biomarkers of colon cancer |
| KR100761031B1 (ko) | 2006-05-27 | 2007-10-04 | 김연수 | 디엔에이 메틸화 현상을 이용한 암 진단용 키트 |
| US20080050738A1 (en) * | 2006-05-31 | 2008-02-28 | Human Genetic Signatures Pty Ltd. | Detection of target nucleic acid |
| US20080085867A1 (en) * | 2006-07-14 | 2008-04-10 | The Johns Hopkins University | Early detection and prognosis of colon cancers |
| ES2599816T3 (es) * | 2006-07-21 | 2017-02-03 | Epigenomics Ag | Métodos relacionados con el gen GLI3 para la detección de cáncer colorrectal |
| US20100278772A1 (en) | 2006-11-20 | 2010-11-04 | The Johns Hopkins University | Dna methylation markers and methods of use |
| EP2099938B1 (en) | 2006-11-24 | 2016-11-16 | Epigenomics AG | Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders |
| JP2010516645A (ja) | 2007-01-18 | 2010-05-20 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング | 癌を治療するためのインテグリンリガンドを用いる特異的療法および薬剤 |
| US20090203011A1 (en) * | 2007-01-19 | 2009-08-13 | Epigenomics Ag | Methods and nucleic acids for analyses of cell proliferative disorders |
| AU2008207110B2 (en) | 2007-01-19 | 2013-10-17 | Epigenomics Ag | Methods and nucleic acids for analyses of cell proliferative disorders |
| WO2008096146A1 (en) | 2007-02-07 | 2008-08-14 | Solexa Limited | Preparation of templates for methylation analysis |
| AU2008229628A1 (en) * | 2007-03-16 | 2008-09-25 | Human Genetic Signatures Pty Ltd | Assay for gene expression |
| WO2008128198A2 (en) | 2007-04-12 | 2008-10-23 | Usc Stevens - University Of Southern California | Dna methylation analysis by digital bisulfite genomic sequencing and digital methylight |
| CA2685376A1 (en) * | 2007-04-25 | 2008-11-06 | John Wayne Cancer Institute | Use of methylated or unmethylated line-1 dna as a cancer marker |
| WO2008140724A1 (en) | 2007-05-08 | 2008-11-20 | Schering Corporation | Methods of treatment using intravenous formulations comprising temozolomide |
| EP2148885A4 (en) * | 2007-05-31 | 2010-12-15 | Monsanto Technology Llc | SOYBEAN POLYMORPHISMS AND METHOD FOR THEIR GENOTYPIZATION |
| EP2154247A4 (en) * | 2007-06-08 | 2010-07-07 | Bio Dixam Llc | PROCESS FOR THE AMPLIFICATION OF A METHYLATED NUCLEIC ACID OR AN UNMETHYLATED NUCLEIC ACID |
| CN101861398A (zh) | 2007-09-17 | 2010-10-13 | 皇家飞利浦电子股份有限公司 | 用于乳腺癌紊乱分析的方法 |
| WO2009037633A2 (en) | 2007-09-17 | 2009-03-26 | Koninklijke Philips Electronics N.V. | Method for the analysis of ovarian cancer disorders |
| WO2009036922A2 (en) | 2007-09-17 | 2009-03-26 | Oncomethylome Sciences Sa | Novel markers for bladder cancer detection |
| EP2215250B1 (en) * | 2007-11-27 | 2013-02-27 | Human Genetic Signatures Pty Ltd | Enzymes for amplification and copying bisulphite modified nucleic acids |
| CA2708163A1 (en) | 2007-12-11 | 2009-06-18 | Epigenomics Ag | Methods and nucleic acids for analyses of cell proliferative disorders |
| CN101903521A (zh) * | 2007-12-20 | 2010-12-01 | 人类遗传标记控股有限公司 | 核酸扩增相关污染物的清除 |
| WO2009089598A2 (en) * | 2008-01-18 | 2009-07-23 | Katholieke Universiteit Leuven | Msmb-gene methylation based diagnosis, staging and prognosis of prostate cancer |
| EP2677041A3 (en) | 2008-02-19 | 2014-04-09 | MDxHealth SA | Detection and prognosis of lung cancer |
| EP2271774B1 (en) | 2008-03-21 | 2018-03-07 | MDxHealth | Detection and prognosis of cervical cancer |
| EP2290064A4 (en) * | 2008-04-14 | 2011-10-12 | Univ Nihon | PROOF OF PROBLEMS FOR PROLIFERATIVE DISEASES |
| WO2009140550A2 (en) | 2008-05-14 | 2009-11-19 | Dermtech International | Diagnosis of melanoma and solar lentigo by nucleic acid analysis |
| EP2304057B1 (en) | 2008-06-17 | 2014-09-17 | Signature Diagnostics AG | Method for the detection of ovarian cancer |
| US20110159499A1 (en) * | 2009-11-25 | 2011-06-30 | Quantalife, Inc. | Methods and compositions for detecting genetic material |
| EP2340314B8 (en) | 2008-10-22 | 2015-02-18 | Illumina, Inc. | Preservation of information related to genomic dna methylation |
| US20120053071A1 (en) | 2008-12-18 | 2012-03-01 | Koninklijke Philips Electronics N.V. | Method for the detection of dna methylation patterns |
| ATE513930T1 (de) | 2009-01-22 | 2011-07-15 | Univ Duesseldorf H Heine | Bestimmung des dna-methylierungsgrads |
| ES2541178T3 (es) | 2009-02-03 | 2015-07-16 | Mdxhealth Sa | Método de detección de cáncer colorrectal |
| US9388471B2 (en) | 2009-03-13 | 2016-07-12 | Mdxhealth Sa | Methylation of the GATA4 gene in urine samples as a marker for bladder cancer detection |
| AU2010225125A1 (en) | 2009-03-17 | 2011-10-20 | Glaxosmithkline Biologicals S.A. | Improved detection of gene expression |
| CA3060721C (en) | 2009-04-20 | 2022-05-17 | Erasmus University Medical Center Rotterdam | Method of diagnosing bladder cancer. |
| EA201101651A1 (ru) | 2009-05-25 | 2012-08-30 | Мерк Патент Гмбх | Непрерывное введение интегриновых лигандов для лечения рака |
| EP2446055A1 (en) | 2009-06-26 | 2012-05-02 | Epigenomics AG | Methods and nucleic acids for analysis of bladder cell proliferative disorders |
| US9624530B2 (en) * | 2009-08-03 | 2017-04-18 | Epigenomics Ag | Methods for preservation of genomic DNA sequence complexity |
| US8642271B2 (en) * | 2009-08-27 | 2014-02-04 | Case Western Reserve University | Aberrant methylation of C6Orf150 DNA sequences in human colorectal cancer |
| WO2011036173A1 (en) | 2009-09-24 | 2011-03-31 | Oncomethylome Sciences S.A. | Detection and prognosis of cervical cancer |
| US8399138B2 (en) * | 2009-10-14 | 2013-03-19 | GM Global Technology Operations LLC | Liquid rechargeable lithium ion battery |
| WO2011051414A1 (en) | 2009-10-28 | 2011-05-05 | Signature Diagnostics Ag | Method for the prognosis of ovarian carcinoma |
| US20110104695A1 (en) | 2009-11-05 | 2011-05-05 | Epigenomics Ag | Methods of predicting therapeutic efficacy of cancer therapy |
| US20110223180A1 (en) * | 2010-03-11 | 2011-09-15 | Aldape Kenneth D | Methods and compositions related to a multi-methylation assay to predict patient outcome |
| WO2011135058A2 (en) | 2010-04-30 | 2011-11-03 | Mdxhealth Sa | Methods for detecting epigenetic modifications |
| AU2011245372A1 (en) | 2010-04-30 | 2012-11-29 | Novartis Ag | Predictive markers useful in the treatment of Fragile X Syndrome (FXS) |
| WO2011150075A2 (en) | 2010-05-25 | 2011-12-01 | The Johns Hopkins University | Compositions and methods for detecting a neoplasia |
| WO2012007137A1 (en) | 2010-07-16 | 2012-01-19 | Merck Patent Gmbh | Peptide for use in the treatment of breast cancer and/or bone metastases |
| US8361720B2 (en) | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| US8916344B2 (en) | 2010-11-15 | 2014-12-23 | Exact Sciences Corporation | Methylation assay |
| ES2829198T3 (es) | 2011-02-02 | 2021-05-31 | Exact Sciences Dev Co Llc | Análisis digital de secuencia de la metilación de ADN |
| WO2012109500A2 (en) | 2011-02-09 | 2012-08-16 | Bio-Rad Laboratories, Inc. | Analysis of nucleic acids |
| WO2012162139A1 (en) | 2011-05-20 | 2012-11-29 | The Regents Of The University Of California | Method to estimate age of individual based on epigenetic markers in biological sample |
| ES2654561T3 (es) | 2011-07-08 | 2018-02-14 | Epigenomics Ag | Procedimientos y ácidos nucleicos para determinar el pronóstico de un sujeto con cáncer |
| BR112014005253B1 (pt) | 2011-09-07 | 2019-11-05 | Human Genetic Signatures Pty Ltd | método de detecção molecular |
| EP2788505B1 (en) | 2011-12-06 | 2017-08-23 | MDxHealth SA | Methods of detecting mutations and epigenetic changes |
| WO2013096661A1 (en) | 2011-12-22 | 2013-06-27 | Illumina, Inc. | Methylation biomarkers for ovarian cancer |
| WO2013131981A1 (en) | 2012-03-08 | 2013-09-12 | Novartis Ag | Predictive markers useful in the diagnosis and treatment of fragile x syndrome (fxs) |
| US9428813B2 (en) | 2012-03-26 | 2016-08-30 | The United States Of America, As Represented By The Secretary, Dept. Of Health & Human Services | DNA methylation analysis for the diagnosis, prognosis and treatment of adrenal neoplasms |
| EP2644705A1 (en) | 2012-03-30 | 2013-10-02 | RWTH Aachen | Biomarker for bladder cancer |
| EP3524693A1 (en) | 2012-04-30 | 2019-08-14 | Raindance Technologies, Inc. | Digital analyte analysis |
| US10233503B2 (en) | 2012-05-24 | 2019-03-19 | Fundació Institut D'investigació Biomèdica De Bellvitge (Idibell) | Method for the identification of the origin of a cancer of unknown primary origin by methylation analysis |
| EP2859353B1 (en) | 2012-06-11 | 2018-09-12 | Medizinische Hochschule Hannover | Susceptibility to and stratification for monoaminergic antidepressants |
| CN105378108A (zh) | 2013-03-13 | 2016-03-02 | 雅培分子公司 | 用于分离核酸的系统和方法 |
| CA2902916C (en) | 2013-03-14 | 2018-08-28 | Mayo Foundation For Medical Education And Research | Detecting neoplasm |
| CN105378107A (zh) | 2013-03-14 | 2016-03-02 | 雅培分子公司 | 多重甲基化-特异性扩增系统和方法 |
| EP2986762B1 (en) | 2013-04-19 | 2019-11-06 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| CN104250663B (zh) * | 2013-06-27 | 2017-09-15 | 北京大学 | 甲基化CpG岛的高通量测序检测方法 |
| GB201322034D0 (en) | 2013-12-12 | 2014-01-29 | Almac Diagnostics Ltd | Prostate cancer classification |
| WO2015124921A1 (en) | 2014-02-19 | 2015-08-27 | The University Court Of The University Of Edinburgh | Methods and uses for determining the presence of inflammatory bowel disease |
| ES2812753T3 (es) | 2014-03-31 | 2021-03-18 | Mayo Found Medical Education & Res | Detección de neoplasma colorectal |
| WO2015169857A1 (en) | 2014-05-07 | 2015-11-12 | Université Libre de Bruxelles | Breast cancer epigenetic markers useful in anthracycline treatment prognosis |
| MA39951A (fr) | 2014-05-09 | 2017-03-15 | Lifecodexx Ag | Détection de l'adn provenant d'un type spécifique de cellule et méthodes associées |
| EP2942400A1 (en) | 2014-05-09 | 2015-11-11 | Lifecodexx AG | Multiplex detection of DNA that originates from a specific cell-type |
| EP2942401A1 (en) | 2014-05-09 | 2015-11-11 | Lifecodexx AG | Detection of DNA that originates from a specific cell-type |
| US10184154B2 (en) | 2014-09-26 | 2019-01-22 | Mayo Foundation For Medical Education And Research | Detecting cholangiocarcinoma |
| CN113981057A (zh) | 2014-12-12 | 2022-01-28 | 精密科学公司 | 用于进行甲基化检测测定的组合物和方法 |
| US9984201B2 (en) | 2015-01-18 | 2018-05-29 | Youhealth Biotech, Limited | Method and system for determining cancer status |
| CN107532124B (zh) | 2015-03-27 | 2022-08-09 | 精密科学公司 | 检测食管疾病 |
| EP3303626B1 (en) | 2015-05-29 | 2020-04-01 | Vito NV | Epigenetic markers for respiratory allergy |
| GB201510684D0 (en) | 2015-06-17 | 2015-08-05 | Almac Diagnostics Ltd | Gene signatures predictive of metastatic disease |
| EP3162899A1 (en) | 2015-10-29 | 2017-05-03 | Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen | Biomarker for breast cancer |
| EP3998353A1 (en) | 2015-10-30 | 2022-05-18 | Exact Sciences Corporation | Multiplex amplification detection assay and isolation and detection of dna from plasma |
| EP3168309B8 (en) | 2015-11-10 | 2020-06-03 | Eurofins LifeCodexx GmbH | Detection of foetal chromosomal aneuploidies using dna regions that are differentially methylated between the foetus and the pregnant female |
| WO2017106481A1 (en) | 2015-12-17 | 2017-06-22 | Illumina, Inc. | Distinguishing methylation levels in complex biological samples |
| WO2017136482A1 (en) | 2016-02-01 | 2017-08-10 | The Board Of Regents Of The University Of Nebraska | Method of identifying important methylome features and use thereof |
| KR102436270B1 (ko) | 2016-05-05 | 2022-08-25 | 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 | 메틸화된 dna 분석에 의한 폐 종양의 검출 |
| DE102016005947B3 (de) | 2016-05-16 | 2017-06-08 | Dimo Dietrich | Verfahren zur Abschätzung der Prognose und zur Prädiktion des Ansprechens auf eine Immuntherapie von Patienten mit malignen Erkrankungen |
| US11685955B2 (en) | 2016-05-16 | 2023-06-27 | Dimo Dietrich | Method for predicting response of patients with malignant diseases to immunotherapy |
| EP3464642A4 (en) | 2016-05-31 | 2020-02-19 | The Regents of the University of California | METHODS FOR EVALUATING, MONITORING AND MODULATING THE AGING PROCESS |
| EP3481951A4 (en) | 2016-07-06 | 2020-08-05 | Youhealth Biotech, Limited | COLON CANCER SPECIFIC METHYLATION MARKERS AND USES OF THESE MARKERS |
| WO2018009707A1 (en) | 2016-07-06 | 2018-01-11 | Youhealth Biotech, Limited | Solid tumor methylation markers and uses thereof |
| WO2018009705A1 (en) | 2016-07-06 | 2018-01-11 | Youhealth Biotech, Limited | Liver cancer methylation markers and uses thereof |
| US10093986B2 (en) | 2016-07-06 | 2018-10-09 | Youhealth Biotech, Limited | Leukemia methylation markers and uses thereof |
| US11396678B2 (en) | 2016-07-06 | 2022-07-26 | The Regent Of The University Of California | Breast and ovarian cancer methylation markers and uses thereof |
| CN106011278B (zh) * | 2016-07-18 | 2020-01-21 | 宁波大学医学院附属医院 | 人胃肠肿瘤中piezo2基因的检测试剂 |
| AU2017299597B2 (en) | 2016-07-19 | 2023-08-24 | Exact Sciences Corporation | Methylated control DNA |
| CN116064795A (zh) | 2016-09-02 | 2023-05-05 | 梅约医学教育与研究基金会 | 确定差异甲基化区域的甲基化状态的方法和试剂盒 |
| EP3336197B1 (en) | 2016-12-16 | 2022-04-06 | Eurofins Genomics Europe Sequencing GmbH | Epigenetic markers and related methods and means for the detection and management of ovarian cancer |
| JP7289264B2 (ja) | 2017-01-27 | 2023-06-09 | エグザクト サイエンシーズ コーポレーション | メチル化ｄｎａの分析による結腸腫瘍の検出 |
| JP7356349B2 (ja) | 2017-02-28 | 2023-10-04 | マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ | 前立腺癌検出 |
| US10513739B2 (en) | 2017-03-02 | 2019-12-24 | Youhealth Oncotech, Limited | Methylation markers for diagnosing hepatocellular carcinoma and lung cancer |
| EP3655418A4 (en) | 2017-06-22 | 2021-05-19 | Triact Therapeutics, Inc. | GLIOBLASTOMA TREATMENT METHODS |
| WO2019012105A1 (en) | 2017-07-14 | 2019-01-17 | Université Libre de Bruxelles | METHOD OF PREDICTING SENSITIVITY TO IMMUNOTHERAPY |
| EP3687501A4 (en) | 2017-09-29 | 2021-06-23 | Triact Therapeutics, Inc. | INIPARIB FORMULATIONS AND THEIR USES |
| US20200277677A1 (en) | 2017-10-06 | 2020-09-03 | Youhealth Oncotech, Limited | Methylation markers for diagnosing cancer |
| DE102017125780B3 (de) | 2017-11-05 | 2018-12-13 | Dimo Dietrich | Verfahren zur Bestimmung des Ansprechens einer malignen Erkrankung auf eine Immuntherapie |
| CN118773309A (zh) | 2017-11-30 | 2024-10-15 | 梅约医学教育与研究基金会 | 用于表征生物样品的方法 |
| WO2019118652A1 (en) | 2017-12-12 | 2019-06-20 | Essenlix Corporation | Sample manipulation and assay with rapid temperature change |
| US10648025B2 (en) | 2017-12-13 | 2020-05-12 | Exact Sciences Development Company, Llc | Multiplex amplification detection assay II |
| WO2019143845A1 (en) | 2018-01-17 | 2019-07-25 | The Regents Of The University Of California | Phenotypic age and dna methylation based biomarkers for life expectancy and morbidity |
| US11976332B2 (en) | 2018-02-14 | 2024-05-07 | Dermtech, Inc. | Gene classifiers and uses thereof in non-melanoma skin cancers |
| WO2020076983A1 (en) | 2018-10-10 | 2020-04-16 | The Regents Of The University Of California | Dna methylation based biomarkers for life expectancy and morbidity |
| EP3867405A4 (en) | 2018-10-17 | 2022-07-20 | The University of Queensland | Epigenetic biomarker and uses therefor |
| ES3041767T3 (en) | 2018-11-16 | 2025-11-14 | Univ Oslo Hf | Methods and compositions for characterizing bladder cancer |
| US20220090200A1 (en) | 2019-01-18 | 2022-03-24 | The Regents Of The University Of California | Dna methylation measurement for mammals based on conserved loci |
| AU2020247911A1 (en) | 2019-03-26 | 2021-11-11 | Dermtech, Llc | Novel gene classifiers and uses thereof in skin cancers |
| AU2020263307B2 (en) * | 2019-04-22 | 2024-02-29 | Behavioral Diagnostics, Llc | Compositions and methods for correcting for cellular admixture in epigenetic analyses |
| US11396679B2 (en) | 2019-05-31 | 2022-07-26 | Universal Diagnostics, S.L. | Detection of colorectal cancer |
| US11001898B2 (en) | 2019-05-31 | 2021-05-11 | Universal Diagnostics, S.L. | Detection of colorectal cancer |
| WO2021050962A1 (en) | 2019-09-11 | 2021-03-18 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Cancer detection and classification |
| JP2022553575A (ja) | 2019-10-31 | 2022-12-23 | マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ | 卵巣癌の検出 |
| US11898199B2 (en) | 2019-11-11 | 2024-02-13 | Universal Diagnostics, S.A. | Detection of colorectal cancer and/or advanced adenomas |
| CN114207153B (zh) | 2020-03-20 | 2024-09-13 | 上海鹍远健康科技有限公司 | 筛查结直肠瘤的方法和试剂盒 |
| US12467096B2 (en) | 2020-05-15 | 2025-11-11 | Universal Diagnostics, S.A. | Methods and systems for identifying methylation biomarkers |
| US12404538B2 (en) | 2020-05-27 | 2025-09-02 | Case Western Reserve University | Compositions and methods for preserving DNA methylation |
| JP2023540161A (ja) * | 2020-06-29 | 2023-09-22 | ペンタベース エーピーエス | メチル化状態の検出 |
| WO2022002424A1 (en) | 2020-06-30 | 2022-01-06 | Universal Diagnostics, S.L. | Systems and methods for detection of multiple cancer types |
| EP4200445A4 (en) | 2020-08-19 | 2025-08-27 | Mayo Found Medical Education & Res | DETECTION OF NON-HODGKIN'S LYMPHOMA |
| AU2022213409A1 (en) | 2021-01-29 | 2023-08-17 | Exact Sciences Corporation | Detecting the presence or absence of multiple types of cancer |
| JP2024537106A (ja) | 2021-09-30 | 2024-10-10 | ティベニクス ソシエテ アノニム | 神経変性疾患または障害の診断方法および進行予測方法 |
| CA3235235A1 (en) | 2021-10-19 | 2023-04-27 | Inserm (Institut National De La Sante Et De La Recherche Medicale) | Dna methylation signature for diagnosing hepatocellular carcinoma |
| EP4170661A1 (en) | 2021-10-19 | 2023-04-26 | Institut National de la Santé et de la Recherche Médicale (INSERM) | Methylation profile analysis using smoothing method |
| WO2023175019A1 (en) | 2022-03-15 | 2023-09-21 | Genknowme S.A. | Method determining the difference between the biological age and the chronological age of a subject |
| EP4299764A1 (en) | 2022-06-28 | 2024-01-03 | Universal Diagnostics, S.A. | Methods for detecting pancreatic cancer using dna methylation markers |
| WO2024105132A1 (en) | 2022-11-16 | 2024-05-23 | Universal Diagnostics, S.A. | Methods for stratification and early detection of advanced adenoma and/or colorectal cancer using dna methylation markers |
| WO2024256726A1 (en) | 2023-06-15 | 2024-12-19 | Genknowme S.A. | Computer implemented method determining a value of allostatic load of a human being |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| AU634533B2 (en) * | 1989-03-21 | 1993-02-25 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | Matrix metalloproteinase inhibitor peptides |
| US5324634A (en) * | 1992-03-31 | 1994-06-28 | The Research Foundation Of State University Of New York | Diagnostic tests measuring gelatinase/inhibitor complexes for detection of aggressive and metastatic cancer |
| IL108978A (en) * | 1994-03-15 | 1998-02-22 | Yissum Res Dev Co | Assay for monitoring the progress of cml |
| US5856094A (en) * | 1995-05-12 | 1999-01-05 | The Johns Hopkins University School Of Medicine | Method of detection of neoplastic cells |
| US5871917A (en) * | 1996-05-31 | 1999-02-16 | North Shore University Hospital Research Corp. | Identification of differentially methylated and mutated nucleic acids |
-
1997
- 1997-04-11 US US08/835,728 patent/US6017704A/en not_active Expired - Lifetime
- 1997-06-03 EP EP06009975A patent/EP1690948A3/en not_active Withdrawn
- 1997-06-03 PT PT97927933T patent/PT954608E/pt unknown
- 1997-06-03 DE DE69735894T patent/DE69735894T2/de not_active Expired - Lifetime
- 1997-06-03 CA CA002257104A patent/CA2257104C/en not_active Expired - Lifetime
- 1997-06-03 JP JP50077998A patent/JP3612080B2/ja not_active Expired - Lifetime
- 1997-06-03 AT AT97927933T patent/ATE326549T1/de active
- 1997-06-03 EP EP97927933A patent/EP0954608B1/en not_active Expired - Lifetime
- 1997-06-03 ES ES97927933T patent/ES2264165T3/es not_active Expired - Lifetime
- 1997-06-03 WO PCT/US1997/009533 patent/WO1997046705A1/en not_active Ceased
- 1997-06-03 DK DK97927933T patent/DK0954608T3/da active
- 1997-06-03 IL IL12734297A patent/IL127342A/en not_active IP Right Cessation
-
2000
- 2000-01-25 US US09/490,558 patent/US6265171B1/en not_active Expired - Lifetime
-
2004
- 2004-05-24 JP JP2004153956A patent/JP3725535B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1690948A2 (en) | 2006-08-16 |
| IL127342A (en) | 2002-07-25 |
| US6017704A (en) | 2000-01-25 |
| EP0954608A4 (en) | 2003-09-10 |
| EP0954608B1 (en) | 2006-05-17 |
| ATE326549T1 (de) | 2006-06-15 |
| JP2000511776A (ja) | 2000-09-12 |
| DK0954608T3 (da) | 2006-08-21 |
| WO1997046705A1 (en) | 1997-12-11 |
| IL127342A0 (en) | 1999-10-28 |
| DE69735894D1 (de) | 2006-06-22 |
| DE69735894T2 (de) | 2007-04-19 |
| PT954608E (pt) | 2006-08-31 |
| CA2257104C (en) | 2008-01-29 |
| JP2004290200A (ja) | 2004-10-21 |
| EP0954608A1 (en) | 1999-11-10 |
| JP3725535B2 (ja) | 2005-12-14 |
| JP3612080B2 (ja) | 2005-01-19 |
| US6265171B1 (en) | 2001-07-24 |
| CA2257104A1 (en) | 1997-12-11 |
| EP1690948A3 (en) | 2006-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2264165T3 (es) | Deteccion especifica de metilacion. | |
| US8785614B2 (en) | Aberrantly methylated genes in pancreatic cancer | |
| US6200756B1 (en) | Methods for identifying methylation patterns in a CpG-containing nucleic acid | |
| US10590465B2 (en) | Enrichment of nucleic acids by complementary capture | |
| CA2132874C (en) | Methods of detecting mammalian nucleic acids isolated from stool specimen and reagents therefor | |
| ES2760919T3 (es) | Diagnóstico de cáncer mediante marcador de metilación | |
| ES2936408T3 (es) | Método de detección múltiple de ADN metilado | |
| BR112014028122B1 (pt) | Método para triar o princípio ou a predisposição ao princípio de neoplasma no intestino grosso ou para monitorar neoplasma no intestino grosso em indivíduo | |
| JP2005204652A (ja) | メチル化特異的プライマー伸長（ｍｓｐｅ）によるメチル化状況を検出するアッセイ | |
| US20030190616A1 (en) | Differentially methylated sequences in pancreatic cancer | |
| US8609343B2 (en) | Detection of bladder cancer | |
| CA2612690C (en) | Methylation specific detection | |
| US9976184B2 (en) | Mutations in pancreatic neoplasms | |
| AU2012278784B2 (en) | Semi-digital ligation assay |