ES2155620T3 - Estabilizacion de plasmidos. - Google Patents
Estabilizacion de plasmidos.Info
- Publication number
- ES2155620T3 ES2155620T3 ES96929443T ES96929443T ES2155620T3 ES 2155620 T3 ES2155620 T3 ES 2155620T3 ES 96929443 T ES96929443 T ES 96929443T ES 96929443 T ES96929443 T ES 96929443T ES 2155620 T3 ES2155620 T3 ES 2155620T3
- Authority
- ES
- Spain
- Prior art keywords
- repressor
- plasmid
- gene
- expressed
- essential
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Abstract
SE DESCRIBE UN SISTEMA QUE UTILIZA UN METODO NOVEDOSO DE TITULACION DEL REPRESOR PARA EL MANTENIMIENTO DE UN PLASMIDO UTIL EN TERAPIA GENICA Y EN LA PRODUCCION DE UNA PROTEINA RECOMBINANTE. EL SISTEMA UTILIZA UNA CELULA HUESPED TRANSFORMADA QUE CONTIENE UN PLASMIDO QUE INCLUYE UN OPERADOR SUSCEPTIBLE DE UNION POR UN REPRESOR EXPRESADO EN TRANS, UN PRIMER GEN CROMOSOMICO QUE CODIFICA EL REPRESOR, Y UN SEGUNDO GEN CROMOSOMICO ASOCIADO FUNCIONALMENTE A UN OPERADOR Y ESENCIAL PARA EL CRECIMIENTO CELULAR, EN EL QUE EL PLASMIDO ESTA PRESENTE EN LA CELULA EN CANTIDAD SUFICIENTE PARA TITULAR EL REPRESOR DE FORMA QUE SE EXPRESE EL GEN ESENCIAL, PERMITIENDO ASI EL CRECIMIENTO DE LA CELULA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9518395.0A GB9518395D0 (en) | 1995-09-08 | 1995-09-08 | Plasmid stabilization |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2155620T3 true ES2155620T3 (es) | 2001-05-16 |
Family
ID=10780420
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96929443T Expired - Lifetime ES2155620T3 (es) | 1995-09-08 | 1996-09-06 | Estabilizacion de plasmidos. |
Country Status (14)
Country | Link |
---|---|
US (1) | US5972708A (es) |
EP (1) | EP0851932B1 (es) |
JP (1) | JP3416888B2 (es) |
KR (1) | KR100356581B1 (es) |
AT (1) | ATE199168T1 (es) |
AU (1) | AU710494B2 (es) |
CA (1) | CA2231784C (es) |
DE (1) | DE69611794T2 (es) |
DK (1) | DK0851932T3 (es) |
ES (1) | ES2155620T3 (es) |
GB (1) | GB9518395D0 (es) |
GR (1) | GR3035806T3 (es) |
PT (1) | PT851932E (es) |
WO (1) | WO1997009435A1 (es) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5763270A (en) * | 1995-06-07 | 1998-06-09 | Genemedicine, Inc. | Plasmid for delivery of nucleic acids to cells and methods of use |
US6720139B1 (en) | 1999-01-27 | 2004-04-13 | Elitra Pharmaceuticals, Inc. | Genes identified as required for proliferation in Escherichia coli |
WO2001034810A2 (en) | 1999-11-09 | 2001-05-17 | Elitra Pharmaceuticals, Inc. | Genes essential for microbial proliferation |
AU2277401A (en) * | 1999-12-23 | 2001-07-09 | Elitra Pharmaceuticals, Inc. | Genes identified as required for proliferation of e. coli |
US6783985B1 (en) | 2000-02-18 | 2004-08-31 | Elitra Pharmaceuticals Inc. | Gene disruption methodologies for drug target discovery |
US20030180953A1 (en) * | 2000-12-29 | 2003-09-25 | Elitra Pharmaceuticals, Inc. | Gene disruption methodologies for drug target discovery |
US20030119013A1 (en) * | 2001-04-23 | 2003-06-26 | Bo Jiang | Identification of essential genes of Aspergillus fumigatus and methods of use |
US20040029129A1 (en) * | 2001-10-25 | 2004-02-12 | Liangsu Wang | Identification of essential genes in microorganisms |
US20030170694A1 (en) * | 2001-12-21 | 2003-09-11 | Daniel Wall | Stabilized nucleic acids in gene and drug discovery and methods of use |
GB0211459D0 (en) * | 2002-05-18 | 2002-06-26 | Cobra Therapeutics Ltd | Plasmid stabilisation in vivo |
WO2005042750A1 (en) * | 2003-10-31 | 2005-05-12 | Novozymes A/S | Method for stable gene-amplification in a bacterial host cell |
GB0327056D0 (en) * | 2003-11-20 | 2003-12-24 | Cobra Biolog Ltd | Plasmid maintenance |
CA2619989C (en) * | 2005-08-23 | 2014-06-17 | National Research Council Of Canada | Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria |
WO2009073551A2 (en) * | 2007-11-30 | 2009-06-11 | Scarab Genomics Llc | Lac expression system |
EP2353607A1 (en) | 2010-02-04 | 2011-08-10 | BioAlliance Pharma | Use of disintegrin domain of an adamalysin for the treatment of psoriasis |
EP3205356A1 (en) | 2016-02-10 | 2017-08-16 | Onkoloski Institut Ljubljana | Plasmid expression vector encoding human interleukin 12 under transcriptional control of p21 promoter and without antibiotic resistance for cancer gene therapy and other uses thereof |
DK3534936T3 (da) * | 2016-11-01 | 2020-11-02 | Novo Nordisk As | Tolerogen dna-vaccine |
WO2019226650A1 (en) | 2018-05-23 | 2019-11-28 | Modernatx, Inc. | Delivery of dna |
AU2021285812A1 (en) | 2020-06-01 | 2023-01-05 | Modernatx, Inc. | Phenylalanine hydroxylase variants and uses thereof |
EP4337177A1 (en) | 2021-05-11 | 2024-03-20 | Modernatx, Inc. | Non-viral delivery of dna for prolonged polypeptide expression in vivo |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK410783D0 (da) * | 1982-09-16 | 1983-09-09 | Benzon A Salfred | Fremgangsmade til stabilisering af plasmider |
GB8308236D0 (en) * | 1983-03-25 | 1983-05-05 | Celltech Ltd | Dna vectors |
DK594084A (da) * | 1984-12-12 | 1986-06-13 | Novo Industri As | Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning |
GB9215550D0 (en) * | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
US5763270A (en) * | 1995-06-07 | 1998-06-09 | Genemedicine, Inc. | Plasmid for delivery of nucleic acids to cells and methods of use |
-
1995
- 1995-09-08 GB GBGB9518395.0A patent/GB9518395D0/en active Pending
-
1996
- 1996-09-06 CA CA2231784A patent/CA2231784C/en not_active Expired - Lifetime
- 1996-09-06 DE DE69611794T patent/DE69611794T2/de not_active Expired - Lifetime
- 1996-09-06 DK DK96929443T patent/DK0851932T3/da active
- 1996-09-06 EP EP96929443A patent/EP0851932B1/en not_active Expired - Lifetime
- 1996-09-06 ES ES96929443T patent/ES2155620T3/es not_active Expired - Lifetime
- 1996-09-06 JP JP51099497A patent/JP3416888B2/ja not_active Expired - Lifetime
- 1996-09-06 PT PT96929443T patent/PT851932E/pt unknown
- 1996-09-06 AU AU68855/96A patent/AU710494B2/en not_active Ceased
- 1996-09-06 AT AT96929443T patent/ATE199168T1/de active
- 1996-09-06 KR KR10-1998-0701773A patent/KR100356581B1/ko not_active IP Right Cessation
- 1996-09-06 WO PCT/GB1996/002208 patent/WO1997009435A1/en active IP Right Grant
-
1998
- 1998-10-21 US US09/176,607 patent/US5972708A/en not_active Expired - Lifetime
-
2001
- 2001-04-27 GR GR20010400654T patent/GR3035806T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
JPH11511985A (ja) | 1999-10-19 |
KR19990044525A (ko) | 1999-06-25 |
WO1997009435A1 (en) | 1997-03-13 |
DE69611794T2 (de) | 2001-08-02 |
EP0851932A1 (en) | 1998-07-08 |
GR3035806T3 (en) | 2001-07-31 |
EP0851932B1 (en) | 2001-02-14 |
US5972708A (en) | 1999-10-26 |
DE69611794D1 (de) | 2001-03-22 |
KR100356581B1 (ko) | 2003-04-10 |
CA2231784A1 (en) | 1997-03-13 |
CA2231784C (en) | 2010-06-22 |
ATE199168T1 (de) | 2001-02-15 |
DK0851932T3 (da) | 2001-06-18 |
AU710494B2 (en) | 1999-09-23 |
AU6885596A (en) | 1997-03-27 |
PT851932E (pt) | 2001-07-31 |
GB9518395D0 (en) | 1995-11-08 |
JP3416888B2 (ja) | 2003-06-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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