ES8800350A1 - Procedimiento para preparar una cepa microbiana transformada mediante un plasmido recombinante. - Google Patents

Procedimiento para preparar una cepa microbiana transformada mediante un plasmido recombinante.

Info

Publication number
ES8800350A1
ES8800350A1 ES549114A ES549114A ES8800350A1 ES 8800350 A1 ES8800350 A1 ES 8800350A1 ES 549114 A ES549114 A ES 549114A ES 549114 A ES549114 A ES 549114A ES 8800350 A1 ES8800350 A1 ES 8800350A1
Authority
ES
Spain
Prior art keywords
plasmid
strain transformed
microbial strain
recombinant plasmid
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES549114A
Other languages
English (en)
Other versions
ES549114A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Publication of ES549114A0 publication Critical patent/ES549114A0/es
Publication of ES8800350A1 publication Critical patent/ES8800350A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

METODO DE PREPARACION DE CEPAS MICROBIANAS MEDIANTE PLASMIDIOS RECOMBINANTES. CONSISTE EN LA TRANSFORMACION DE UNA CEPA MICROBIANA CON UN PLASMIDO RECOMBINANTE POR TRATAMIENTO DE LA MISMA CON UN ENZIMA DIGESTORA DE LA PARED CELULAR DE LA LEVADURA A UNA TEMPERATURA DE 30GC, LAVADO DE LA CEPA TRATADA CON UNA DISOLUCION ISOTONICA DE SORBITOL; SUSPENSION DE LA CEPA EN UNA DISOLUCION ISOTONICA DE CACL2 QUE CONTIENE SORBITOL; ADICION DE 10-20 MU/ML DE ADN RECOMBINANTE; INCUBACION DE LA MEZCLA 5-15 MINUTOS A TEMPERATURA AMBIENTE; AÑADIR DISOLUCION DE CACL2 QUE CONTIENE 30-40 POR CIEN DE POLIETILENGLICOL A PH 7-8 Y TRATAMIENTO A TEMPERATURA AMBIENTE; CENTRIFUGACION PARA SEPARAR LA CEPA TRANSFORMADA Y CULTIVARLA EN UN MEDIO SIN AMINOACIDO PARA CLONARLA.
ES549114A 1984-11-05 1985-11-05 Procedimiento para preparar una cepa microbiana transformada mediante un plasmido recombinante. Expired ES8800350A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP23268384 1984-11-05

Publications (2)

Publication Number Publication Date
ES549114A0 ES549114A0 (es) 1987-11-16
ES8800350A1 true ES8800350A1 (es) 1987-11-16

Family

ID=16943153

Family Applications (1)

Application Number Title Priority Date Filing Date
ES549114A Expired ES8800350A1 (es) 1984-11-05 1985-11-05 Procedimiento para preparar una cepa microbiana transformada mediante un plasmido recombinante.

Country Status (7)

Country Link
US (1) US4757021A (es)
EP (1) EP0180958B1 (es)
JP (1) JPS61268184A (es)
KR (1) KR860004148A (es)
CA (1) CA1264687A (es)
DE (1) DE3577215D1 (es)
ES (1) ES8800350A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0254249B1 (en) * 1986-07-22 1992-08-19 Ciba-Geigy Ag Preparation of binding factor related polypeptides

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU560472B2 (en) * 1981-08-25 1987-04-09 Celltech Limited Yeast expression vectors
EP0105149B1 (en) * 1982-08-16 1990-05-16 Science and Technology Agency, Minister's Secretariat, Director of Finance Division Recombinant plasmid containing hepatitis b virus gene, yeast transformed with said recombinant plasmid, and production of hepatitis b virus surface antigen

Also Published As

Publication number Publication date
KR860004148A (ko) 1986-06-18
DE3577215D1 (de) 1990-05-23
JPS61268184A (ja) 1986-11-27
ES549114A0 (es) 1987-11-16
EP0180958B1 (en) 1990-04-18
US4757021A (en) 1988-07-12
CA1264687A (en) 1990-01-23
EP0180958A1 (en) 1986-05-14

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970303