DK0851932T3 - Plasmidstabilisering - Google Patents

Plasmidstabilisering

Info

Publication number
DK0851932T3
DK0851932T3 DK96929443T DK96929443T DK0851932T3 DK 0851932 T3 DK0851932 T3 DK 0851932T3 DK 96929443 T DK96929443 T DK 96929443T DK 96929443 T DK96929443 T DK 96929443T DK 0851932 T3 DK0851932 T3 DK 0851932T3
Authority
DK
Denmark
Prior art keywords
repressor
plasmid
gene
expressed
essential
Prior art date
Application number
DK96929443T
Other languages
English (en)
Inventor
David John Sherratt
Steven Geraint Williams
Julian Alexis John Hanak
Original Assignee
Cobra Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cobra Therapeutics Ltd filed Critical Cobra Therapeutics Ltd
Application granted granted Critical
Publication of DK0851932T3 publication Critical patent/DK0851932T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK96929443T 1995-09-08 1996-09-06 Plasmidstabilisering DK0851932T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9518395.0A GB9518395D0 (en) 1995-09-08 1995-09-08 Plasmid stabilization
PCT/GB1996/002208 WO1997009435A1 (en) 1995-09-08 1996-09-06 Plasmid stabilization

Publications (1)

Publication Number Publication Date
DK0851932T3 true DK0851932T3 (da) 2001-06-18

Family

ID=10780420

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96929443T DK0851932T3 (da) 1995-09-08 1996-09-06 Plasmidstabilisering

Country Status (14)

Country Link
US (1) US5972708A (da)
EP (1) EP0851932B1 (da)
JP (1) JP3416888B2 (da)
KR (1) KR100356581B1 (da)
AT (1) ATE199168T1 (da)
AU (1) AU710494B2 (da)
CA (1) CA2231784C (da)
DE (1) DE69611794T2 (da)
DK (1) DK0851932T3 (da)
ES (1) ES2155620T3 (da)
GB (1) GB9518395D0 (da)
GR (1) GR3035806T3 (da)
PT (1) PT851932E (da)
WO (1) WO1997009435A1 (da)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
AU2041901A (en) * 1999-11-09 2001-06-06 Elitra Pharmaceuticals, Inc. Genes essential for microbial proliferation and antisense thereto
KR20030003221A (ko) * 1999-12-23 2003-01-09 엘리트라 파마슈티컬즈, 인코포레이티드 대장균 증식에 필요한 유전자
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
US20030180953A1 (en) * 2000-12-29 2003-09-25 Elitra Pharmaceuticals, Inc. Gene disruption methodologies for drug target discovery
WO2002086090A2 (en) * 2001-04-23 2002-10-31 Elitra Pharmaceuticals, Inc. Identification of essential genes of aspegillus fumigatus and methods of use
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms
US20030170694A1 (en) * 2001-12-21 2003-09-11 Daniel Wall Stabilized nucleic acids in gene and drug discovery and methods of use
GB0211459D0 (en) * 2002-05-18 2002-06-26 Cobra Therapeutics Ltd Plasmid stabilisation in vivo
US7700322B2 (en) 2003-10-31 2010-04-20 Novozymes A/S Method for stable gene-amplification in a bacterial host cell
GB0327056D0 (en) * 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance
CA2619989C (en) * 2005-08-23 2014-06-17 National Research Council Of Canada Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria
WO2009073551A2 (en) * 2007-11-30 2009-06-11 Scarab Genomics Llc Lac expression system
EP2353607A1 (en) 2010-02-04 2011-08-10 BioAlliance Pharma Use of disintegrin domain of an adamalysin for the treatment of psoriasis
EP3205356A1 (en) 2016-02-10 2017-08-16 Onkoloski Institut Ljubljana Plasmid expression vector encoding human interleukin 12 under transcriptional control of p21 promoter and without antibiotic resistance for cancer gene therapy and other uses thereof
PL3534936T3 (pl) * 2016-11-01 2021-01-25 Novo Nordisk A/S Szczepionka tolerogennego dna
US20210346306A1 (en) 2018-05-23 2021-11-11 Modernatx, Inc. Delivery of dna
CA3184474A1 (en) 2020-06-01 2021-12-09 Modernatx, Inc. Phenylalanine hydroxylase variants and uses thereof
EP4337177A1 (en) 2021-05-11 2024-03-20 Modernatx, Inc. Non-viral delivery of dna for prolonged polypeptide expression in vivo

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK410783D0 (da) * 1982-09-16 1983-09-09 Benzon A Salfred Fremgangsmade til stabilisering af plasmider
GB8308236D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Dna vectors
DK594084A (da) * 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
GB9215550D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use

Also Published As

Publication number Publication date
US5972708A (en) 1999-10-26
PT851932E (pt) 2001-07-31
DE69611794T2 (de) 2001-08-02
ES2155620T3 (es) 2001-05-16
WO1997009435A1 (en) 1997-03-13
KR100356581B1 (ko) 2003-04-10
ATE199168T1 (de) 2001-02-15
KR19990044525A (ko) 1999-06-25
GB9518395D0 (en) 1995-11-08
AU710494B2 (en) 1999-09-23
CA2231784A1 (en) 1997-03-13
AU6885596A (en) 1997-03-27
EP0851932B1 (en) 2001-02-14
JP3416888B2 (ja) 2003-06-16
GR3035806T3 (en) 2001-07-31
JPH11511985A (ja) 1999-10-19
DE69611794D1 (de) 2001-03-22
CA2231784C (en) 2010-06-22
EP0851932A1 (en) 1998-07-08

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