ES2100376T3 - Procedimiento de produccion de citidina difosfato colina. - Google Patents

Procedimiento de produccion de citidina difosfato colina.

Info

Publication number
ES2100376T3
ES2100376T3 ES93101323T ES93101323T ES2100376T3 ES 2100376 T3 ES2100376 T3 ES 2100376T3 ES 93101323 T ES93101323 T ES 93101323T ES 93101323 T ES93101323 T ES 93101323T ES 2100376 T3 ES2100376 T3 ES 2100376T3
Authority
ES
Spain
Prior art keywords
choline
hill
production procedure
cytidine
cytidine diphosphate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93101323T
Other languages
English (en)
Inventor
Akihiko Maruyama
Tatsuro Fujio
Sadao Teshiba
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Application granted granted Critical
Publication of ES2100376T3 publication Critical patent/ES2100376T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE DESCRIBE UN PROCESO PARA LA PRODUCCION DE COLINA DIFOSFATO CITIDINA, EL PROCESO COMPRENDE LA REALIZACION DE UNA REACCION ENZIMATICA UTILIZANDO MICROORGANISMOS QUE DISPONEN DE ACTIVIDAD ENZIMATICA DE SINTESIS CITIDINA COLINEFOSFATO CITIDILTRANSFERASA (CCT) Y COLINA QUINASA (CKI) Y UN MICROORGANISMO CAPAZ DE PRODUCIR URIDINA A PARTIR DE ACIDO OROTICO COMO FUENTE DE ENZIMA, Y ACIDO OROTICO Y COLINA Y/O FOSFORIL COLINA COMO SUBSTRATOS, PERMITIENDO COLINA DIFOSFATO CITIDINA PARA SER ACUMULADA EN LA MEZCLA DE REACCION, Y RECUPERACION DE LA COLINA DIFOSFATO CITIDINA A PARTIR DE LA MEZCLA DE REACCION MENCIONADA.
ES93101323T 1992-01-30 1993-01-28 Procedimiento de produccion de citidina difosfato colina. Expired - Lifetime ES2100376T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1485892 1992-01-30

Publications (1)

Publication Number Publication Date
ES2100376T3 true ES2100376T3 (es) 1997-06-16

Family

ID=11872731

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93101323T Expired - Lifetime ES2100376T3 (es) 1992-01-30 1993-01-28 Procedimiento de produccion de citidina difosfato colina.

Country Status (8)

Country Link
US (2) US6387667B1 (es)
EP (1) EP0553821B1 (es)
KR (1) KR0177841B1 (es)
CN (1) CN1060215C (es)
AT (1) ATE150487T1 (es)
DE (1) DE69308908D1 (es)
ES (1) ES2100376T3 (es)
HK (1) HK1000604A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1110568C (zh) * 1995-10-20 2003-06-04 傅责中 富含ctp水溶液的生产工艺
KR100556051B1 (ko) * 1996-09-13 2006-05-23 교와 핫꼬 고교 가부시끼가이샤 당뉴클레오티드류및복합당질의제조방법
CN100436593C (zh) 1996-09-17 2008-11-26 协和发酵工业株式会社 复合糖类的制备方法
DE60328595D1 (de) * 2002-05-08 2009-09-10 Kyowa Hakko Bio Co Ltd Verfahren zur herstellung von cytidin 5'-diphosphatcholin
JP2006512066A (ja) * 2002-12-23 2006-04-13 サイノファーム・バイオテック・リミテッド リボヌクレオチドの酵素的還元によるデオキシリボヌクレオチド製造
TW200741005A (en) 2005-08-10 2007-11-01 Kyowa Hakko Kogyo Kk A purification method of cytidine diphosphate
EP2376619A4 (en) 2008-12-15 2012-07-04 Greenlight Biosciences Inc METHODS FOR CONTROLLING FLOWS IN METABOLIC PATHWAYS
WO2010074760A1 (en) * 2008-12-22 2010-07-01 Greenlight Biosciences Compositions and methods for the production of a compound
CN101792786B (zh) * 2010-03-04 2012-07-11 南京工业大学 一种定向催化合成胞苷磷酰化合物的方法
KR20130071433A (ko) 2010-05-07 2013-06-28 그린라이트 바이오사이언시스, 아이엔씨. 효소의 재배치를 통한 대사 경로에서의 흐름의 제어 방법
KR101988063B1 (ko) 2010-08-31 2019-09-30 그린라이트 바이오사이언시스, 아이엔씨. 프로테아제 조작을 통한 대사 경로에서 유동의 제어 방법
CN102212096A (zh) * 2011-04-08 2011-10-12 南通香地生物有限公司 利用复合溶剂及缚酸剂制作5′-胞苷酸的方法
WO2013036787A2 (en) 2011-09-09 2013-03-14 Greenlight Biosciences, Inc. Cell-free preparation of carbapenems
EP3460069B1 (en) 2013-08-05 2021-10-20 Greenlight Biosciences, Inc. Therapeutically active compounds and their methods of use
CN103483408B (zh) * 2013-09-18 2015-07-15 南京工业大学 一种用微通道反应装置连续生产5′-核苷酸的方法
KR20180002636A (ko) 2015-03-30 2018-01-08 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
CN104774799B (zh) * 2015-04-17 2018-01-30 南京工业大学 一株表达胆碱激酶及磷酰胆碱胞苷转移酶的基因工程菌及其构建方法与应用
CN105463042A (zh) * 2015-10-23 2016-04-06 苏州天马精细化学品股份有限公司 一种生物酶催化制备胞二磷胆碱的方法
KR20230079463A (ko) 2016-04-06 2023-06-07 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
CN110300800A (zh) 2017-01-06 2019-10-01 绿光生物科技股份有限公司 糖的无细胞生产
CN109207415B (zh) * 2017-07-07 2022-09-16 苏州华赛生物工程技术有限公司 一种生产胞二磷胆碱的重组微生物及生产胞二磷胆碱的方法
KR102571743B1 (ko) 2017-10-11 2023-08-29 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물
CN108424859B (zh) * 2017-11-23 2021-09-10 华东理工大学 生产胞磷胆碱的基因工程菌的构建与应用
CN115725536A (zh) * 2021-08-27 2023-03-03 北京天开易达生物科技有限公司 一种双功能酶、双功能酶突变体及其应用
CN116790466B (zh) * 2023-07-19 2023-11-03 山东理工大学 一种工程改造枯草芽孢杆菌发酵生产胞磷胆碱的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4840757B1 (es) 1969-02-21 1973-12-03
JPS4840758B1 (es) 1969-03-24 1973-12-03
JPS4841555B1 (es) * 1969-08-04 1973-12-07
USRE28886E (en) * 1969-08-04 1976-06-29 Kyowa Hakko Kogyo Co., Ltd. Method for preparing cytidine diphosphate choline
JPS5140159B1 (es) 1969-11-26 1976-11-01
JPS521175Y2 (es) 1971-06-02 1977-01-12
JPS53109996A (en) 1977-03-07 1978-09-26 Hikari Kimura Production of cytidine diphosphoric acid choline
JPS599158B2 (ja) 1977-07-01 1984-02-29 協和醗酵工業株式会社 シチジン二リン酸コリンの製造法
JPS601199A (ja) 1983-06-16 1985-01-07 Kohjin Co Ltd シチジン−５′−ジリン酸コリンの製造方法
US4774180A (en) * 1986-02-26 1988-09-27 Massachusetts Institute Of Technology Construction and application of polyproteins
IT1188178B (it) * 1985-07-05 1988-01-07 Bioresearch Spa Sali della citidin-difospocolina particolarmente idonei per uso orale
JP2724825B2 (ja) 1987-06-15 1998-03-09 旭化成工業株式会社 シチジンジリン酸コリンの製造方法
JPH0710235B2 (ja) 1987-10-19 1995-02-08 協和醗酵工業株式会社 発酵法によるオロット酸の製造法

Also Published As

Publication number Publication date
CN1074938A (zh) 1993-08-04
US6387667B1 (en) 2002-05-14
ATE150487T1 (de) 1997-04-15
KR0177841B1 (ko) 1999-04-01
CN1060215C (zh) 2001-01-03
EP0553821B1 (en) 1997-03-19
DE69308908D1 (de) 1997-04-24
US7226767B2 (en) 2007-06-05
KR930016543A (ko) 1993-08-26
HK1000604A1 (en) 1998-04-09
EP0553821A1 (en) 1993-08-04
US20020151036A1 (en) 2002-10-17

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