ATE150487T1 - Verfahren zur herstellung von cytidin-diphosphat- cholin - Google Patents
Verfahren zur herstellung von cytidin-diphosphat- cholinInfo
- Publication number
- ATE150487T1 ATE150487T1 AT93101323T AT93101323T ATE150487T1 AT E150487 T1 ATE150487 T1 AT E150487T1 AT 93101323 T AT93101323 T AT 93101323T AT 93101323 T AT93101323 T AT 93101323T AT E150487 T1 ATE150487 T1 AT E150487T1
- Authority
- AT
- Austria
- Prior art keywords
- choline
- cytidine
- producing
- diphosphat
- cytidine diphosphate
- Prior art date
Links
- 229960001231 choline Drugs 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title 1
- PXQPEWDEAKTCGB-UHFFFAOYSA-N orotic acid Chemical compound OC(=O)C1=CC(=O)NC(=O)N1 PXQPEWDEAKTCGB-UHFFFAOYSA-N 0.000 abstract 4
- RZZPDXZPRHQOCG-OJAKKHQRSA-M CDP-choline(1-) Chemical compound O[C@@H]1[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OCC[N+](C)(C)C)O[C@H]1N1C(=O)N=C(N)C=C1 RZZPDXZPRHQOCG-OJAKKHQRSA-M 0.000 abstract 3
- 229960001284 citicoline Drugs 0.000 abstract 3
- 102000002745 Choline Kinase Human genes 0.000 abstract 2
- 108010018888 Choline kinase Proteins 0.000 abstract 2
- 102000015083 Choline-Phosphate Cytidylyltransferase Human genes 0.000 abstract 2
- 108010078853 Choline-Phosphate Cytidylyltransferase Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 244000005700 microbiome Species 0.000 abstract 2
- 229960005010 orotic acid Drugs 0.000 abstract 2
- 239000011541 reaction mixture Substances 0.000 abstract 2
- PCDQPRRSZKQHHS-UHFFFAOYSA-N Cytidine 5'-triphosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 PCDQPRRSZKQHHS-UHFFFAOYSA-N 0.000 abstract 1
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- PGAVKCOVUIYSFO-XVFCMESISA-N UTP Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 PGAVKCOVUIYSFO-XVFCMESISA-N 0.000 abstract 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- YHHSONZFOIEMCP-UHFFFAOYSA-O phosphocholine Chemical compound C[N+](C)(C)CCOP(O)(O)=O YHHSONZFOIEMCP-UHFFFAOYSA-O 0.000 abstract 1
- 229950004354 phosphorylcholine Drugs 0.000 abstract 1
- 101150054232 pyrG gene Proteins 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- PGAVKCOVUIYSFO-UHFFFAOYSA-N uridine-triphosphate Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 PGAVKCOVUIYSFO-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1485892 | 1992-01-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE150487T1 true ATE150487T1 (de) | 1997-04-15 |
Family
ID=11872731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT93101323T ATE150487T1 (de) | 1992-01-30 | 1993-01-28 | Verfahren zur herstellung von cytidin-diphosphat- cholin |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6387667B1 (de) |
| EP (1) | EP0553821B1 (de) |
| KR (1) | KR0177841B1 (de) |
| CN (1) | CN1060215C (de) |
| AT (1) | ATE150487T1 (de) |
| DE (1) | DE69308908D1 (de) |
| ES (1) | ES2100376T3 (de) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1110568C (zh) * | 1995-10-20 | 2003-06-04 | 傅责中 | 富含ctp水溶液的生产工艺 |
| KR100556051B1 (ko) * | 1996-09-13 | 2006-05-23 | 교와 핫꼬 고교 가부시끼가이샤 | 당뉴클레오티드류및복합당질의제조방법 |
| CN100342025C (zh) | 1996-09-17 | 2007-10-10 | 协和发酵工业株式会社 | 糖核苷酸类及复合糖类的制备方法 |
| CN100513576C (zh) * | 2002-05-08 | 2009-07-15 | 协和发酵生化株式会社 | 胞苷5’-二磷酸胆碱的制备方法 |
| US20040214291A1 (en) * | 2002-12-23 | 2004-10-28 | Scinopharm Biotech, Ltd. | Deoxyribonucleotides manufacturing by enzymatic reduction of ribonucleotides |
| TW200741005A (en) | 2005-08-10 | 2007-11-01 | Kyowa Hakko Kogyo Kk | A purification method of cytidine diphosphate |
| WO2010077806A1 (en) | 2008-12-15 | 2010-07-08 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways |
| CN105132387A (zh) * | 2008-12-22 | 2015-12-09 | 绿光生物科学公司 | 用于制造化合物的组合物和方法 |
| CN101792786B (zh) * | 2010-03-04 | 2012-07-11 | 南京工业大学 | 一种定向催化合成胞苷磷酰化合物的方法 |
| US8956833B2 (en) | 2010-05-07 | 2015-02-17 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through enzyme relocation |
| EP2611922A1 (de) | 2010-08-31 | 2013-07-10 | Greenlight Biosciences, Inc. | Verfahren zur flusssteuerung bei stoffwechselwegen durch proteasemanipulation |
| CN102212096A (zh) * | 2011-04-08 | 2011-10-12 | 南通香地生物有限公司 | 利用复合溶剂及缚酸剂制作5′-胞苷酸的方法 |
| EP2753702B1 (de) | 2011-09-09 | 2021-12-15 | GreenLight Biosciences, Inc. | Zellfreie herstellung von carbapenemen |
| CA2919383A1 (en) | 2013-08-05 | 2015-02-12 | Greenlight Biosciences, Inc. | Engineered proteins with a protease cleavage site |
| CN103483408B (zh) * | 2013-09-18 | 2015-07-15 | 南京工业大学 | 一种用微通道反应装置连续生产5′-核苷酸的方法 |
| CR20170485A (es) | 2015-03-30 | 2018-02-05 | Greenlight Biosciences Inc | Producción de ácido ribonucleico libre de células |
| CN104774799B (zh) * | 2015-04-17 | 2018-01-30 | 南京工业大学 | 一株表达胆碱激酶及磷酰胆碱胞苷转移酶的基因工程菌及其构建方法与应用 |
| CN105463042A (zh) * | 2015-10-23 | 2016-04-06 | 苏州天马精细化学品股份有限公司 | 一种生物酶催化制备胞二磷胆碱的方法 |
| WO2017176963A1 (en) | 2016-04-06 | 2017-10-12 | Greenlight Biosciences, Inc. | Cell-free production of ribonucleic acid |
| AU2018205503A1 (en) | 2017-01-06 | 2019-07-25 | Greenlight Biosciences, Inc. | Cell-free production of sugars |
| CN109207415B (zh) * | 2017-07-07 | 2022-09-16 | 苏州华赛生物工程技术有限公司 | 一种生产胞二磷胆碱的重组微生物及生产胞二磷胆碱的方法 |
| SG11202003192UA (en) | 2017-10-11 | 2020-05-28 | Greenlight Biosciences Inc | Methods and compositions for nucleoside triphosphate and ribonucleic acid production |
| CN108424859B (zh) * | 2017-11-23 | 2021-09-10 | 华东理工大学 | 生产胞磷胆碱的基因工程菌的构建与应用 |
| US11713330B2 (en) * | 2019-04-16 | 2023-08-01 | Helixbind, Inc. | Methods and devices for ultrasensitive direct detection of microorganisms |
| CN115725536B (zh) * | 2021-08-27 | 2025-05-02 | 安徽古特生物科技有限公司 | 一种双功能酶、双功能酶突变体及其应用 |
| CN116790466B (zh) * | 2023-07-19 | 2023-11-03 | 山东理工大学 | 一种工程改造枯草芽孢杆菌发酵生产胞磷胆碱的方法 |
| CN120192906B (zh) * | 2025-05-27 | 2025-09-19 | 广东筑美生物医疗科技有限公司 | 一种生产胞磷胆碱的大肠杆菌重组工程菌及其构建方法与应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4840757B1 (de) | 1969-02-21 | 1973-12-03 | ||
| JPS4840758B1 (de) | 1969-03-24 | 1973-12-03 | ||
| USRE28886E (en) * | 1969-08-04 | 1976-06-29 | Kyowa Hakko Kogyo Co., Ltd. | Method for preparing cytidine diphosphate choline |
| JPS4841555B1 (de) * | 1969-08-04 | 1973-12-07 | ||
| JPS5140159B1 (de) | 1969-11-26 | 1976-11-01 | ||
| JPS521175Y2 (de) | 1971-06-02 | 1977-01-12 | ||
| JPS53109996A (en) | 1977-03-07 | 1978-09-26 | Hikari Kimura | Production of cytidine diphosphoric acid choline |
| JPS599158B2 (ja) | 1977-07-01 | 1984-02-29 | 協和醗酵工業株式会社 | シチジン二リン酸コリンの製造法 |
| JPS601199A (ja) | 1983-06-16 | 1985-01-07 | Kohjin Co Ltd | シチジン−５′−ジリン酸コリンの製造方法 |
| US4774180A (en) * | 1986-02-26 | 1988-09-27 | Massachusetts Institute Of Technology | Construction and application of polyproteins |
| IT1188178B (it) * | 1985-07-05 | 1988-01-07 | Bioresearch Spa | Sali della citidin-difospocolina particolarmente idonei per uso orale |
| JP2724825B2 (ja) | 1987-06-15 | 1998-03-09 | 旭化成工業株式会社 | シチジンジリン酸コリンの製造方法 |
| JPH0710235B2 (ja) | 1987-10-19 | 1995-02-08 | 協和醗酵工業株式会社 | 発酵法によるオロット酸の製造法 |
-
1993
- 1993-01-27 KR KR1019930000998A patent/KR0177841B1/ko not_active Expired - Lifetime
- 1993-01-28 US US08/014,012 patent/US6387667B1/en not_active Expired - Lifetime
- 1993-01-28 ES ES93101323T patent/ES2100376T3/es not_active Expired - Lifetime
- 1993-01-28 AT AT93101323T patent/ATE150487T1/de active
- 1993-01-28 DE DE69308908T patent/DE69308908D1/de not_active Expired - Lifetime
- 1993-01-28 EP EP93101323A patent/EP0553821B1/de not_active Expired - Lifetime
- 1993-01-29 CN CN93100917A patent/CN1060215C/zh not_active Expired - Lifetime
-
2002
- 2002-04-02 US US10/113,573 patent/US7226767B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0553821B1 (de) | 1997-03-19 |
| US7226767B2 (en) | 2007-06-05 |
| EP0553821A1 (de) | 1993-08-04 |
| HK1000604A1 (en) | 1998-04-09 |
| US6387667B1 (en) | 2002-05-14 |
| US20020151036A1 (en) | 2002-10-17 |
| KR0177841B1 (ko) | 1999-04-01 |
| DE69308908D1 (de) | 1997-04-24 |
| ES2100376T3 (es) | 1997-06-16 |
| KR930016543A (ko) | 1993-08-26 |
| CN1060215C (zh) | 2001-01-03 |
| CN1074938A (zh) | 1993-08-04 |
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