DE69114844D1 - Enzymatisches amplifizierungsverfahren in vitro. - Google Patents

Enzymatisches amplifizierungsverfahren in vitro.

Info

Publication number
DE69114844D1
DE69114844D1 DE69114844T DE69114844T DE69114844D1 DE 69114844 D1 DE69114844 D1 DE 69114844D1 DE 69114844 T DE69114844 T DE 69114844T DE 69114844 T DE69114844 T DE 69114844T DE 69114844 D1 DE69114844 D1 DE 69114844D1
Authority
DE
Germany
Prior art keywords
amplification
vitro
reamplification
pcr reaction
resulting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69114844T
Other languages
English (en)
Other versions
DE69114844T2 (de
Inventor
Pascal Brandys
Auriol Luc D
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Biodevelopment SAS
Original Assignee
Serono Genetics Institute SA
Genset SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serono Genetics Institute SA, Genset SA filed Critical Serono Genetics Institute SA
Publication of DE69114844D1 publication Critical patent/DE69114844D1/de
Application granted granted Critical
Publication of DE69114844T2 publication Critical patent/DE69114844T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69114844T 1990-06-29 1991-06-27 Enzymatisches amplifizierungsverfahren in vitro. Expired - Fee Related DE69114844T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9008247A FR2663949A1 (fr) 1990-06-29 1990-06-29 Perfectionnements a un procede d'amplification enzymatique in vitro.
PCT/FR1991/000513 WO1992000384A1 (fr) 1990-06-29 1991-06-27 Perfectionnements a un procede d'amplification enzymatique in vitro

Publications (2)

Publication Number Publication Date
DE69114844D1 true DE69114844D1 (de) 1996-01-04
DE69114844T2 DE69114844T2 (de) 1996-08-01

Family

ID=9398171

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69114844T Expired - Fee Related DE69114844T2 (de) 1990-06-29 1991-06-27 Enzymatisches amplifizierungsverfahren in vitro.

Country Status (6)

Country Link
EP (1) EP0536214B1 (de)
AT (1) ATE130634T1 (de)
AU (1) AU8092791A (de)
DE (1) DE69114844T2 (de)
FR (1) FR2663949A1 (de)
WO (1) WO1992000384A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856088A (en) * 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
US5650302A (en) * 1990-05-01 1997-07-22 Amgen Inc. Method for reducing carryover contamination in an amplification procedure
CA2058232A1 (en) * 1991-01-22 1992-07-23 Joseph A. Walder Process to prevent contamination by amplified nucleic acid sequence
CA2101119A1 (en) * 1992-08-17 1994-02-18 George T. Walker Exonuclease decontamination method
CA2159103C (en) * 1993-03-26 2002-03-12 Sherrol H. Mcdonough Detection of human immunodeficiency virus type 1
US5605796A (en) * 1994-07-19 1997-02-25 Behringwerke Ag Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates
ZA956776B (en) 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
US6495320B1 (en) * 1999-07-21 2002-12-17 Affymetrix, Inc. Even length proportional amplification of nucleic acids

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE68911648T2 (de) * 1988-03-24 1994-06-23 Univ Iowa Res Found Katalytische hybridisierungs-systeme zum nachweis von nukleinsäuresequenzen, die auf deren aktivität als kofaktoren in katalytischen reaktionen basieren in denen eine komplementäre, markierte nukleinsäureprobe gespalten wird.
EP0451141B1 (de) * 1988-05-20 1998-11-18 F. Hoffmann-La Roche Ag Befestigung von sequenzspezifischen proben
AU4181089A (en) * 1988-08-01 1990-03-05 George D. Cimino Identification of allele specific nucleic acid sequences by hybridization with crosslinkable oligonucleotide probes
DE68926460T2 (de) * 1988-12-05 1996-09-12 Salk Inst For Biological Studi Systeme für amplifikation/nachweis von nukleinsäuren
AU5640090A (en) * 1989-03-21 1990-11-05 Collaborative Research Inc. A dna diagnostic test using an exonuclease activity
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
FR2649122B1 (fr) * 1989-07-03 1991-10-18 Pasteur Institut Perfectionnements apportes aux techniques d'amplification par reaction de polymerisation en chaine (pcr)
DE69024286T2 (de) * 1989-09-01 1996-05-30 Life Technologies Inc Verfahren zur Überprüfung der Kontamination von oligonukleotidabhängigen Nukleinsäure-Amplifikationsreaktionen

Also Published As

Publication number Publication date
EP0536214B1 (de) 1995-11-22
AU8092791A (en) 1992-01-23
WO1992000384A1 (fr) 1992-01-09
FR2663949B1 (de) 1995-01-20
EP0536214A1 (de) 1993-04-14
FR2663949A1 (fr) 1992-01-03
DE69114844T2 (de) 1996-08-01
ATE130634T1 (de) 1995-12-15

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee