DE69329636D1 - Verfahren zur Vervielfältigung von Nukleinsäuresequenzen - Google Patents

Verfahren zur Vervielfältigung von Nukleinsäuresequenzen

Info

Publication number
DE69329636D1
DE69329636D1 DE69329636T DE69329636T DE69329636D1 DE 69329636 D1 DE69329636 D1 DE 69329636D1 DE 69329636 T DE69329636 T DE 69329636T DE 69329636 T DE69329636 T DE 69329636T DE 69329636 D1 DE69329636 D1 DE 69329636D1
Authority
DE
Germany
Prior art keywords
amplification
nucleic acid
acid sequences
oligonucleotides
kits
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69329636T
Other languages
English (en)
Other versions
DE69329636T2 (de
Inventor
Sherrol Mcdonough
Daniel Kacian
Nanibhushan Dattagupta
Diane Mcallister
Philip Hammond
Thomas Ryder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of DE69329636D1 publication Critical patent/DE69329636D1/de
Publication of DE69329636T2 publication Critical patent/DE69329636T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
DE69329636T 1992-08-04 1993-08-04 Verfahren zur Vervielfältigung von Nukleinsäuresequenzen Expired - Lifetime DE69329636T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US92540592A 1992-08-04 1992-08-04

Publications (2)

Publication Number Publication Date
DE69329636D1 true DE69329636D1 (de) 2000-12-14
DE69329636T2 DE69329636T2 (de) 2001-03-01

Family

ID=25451694

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69334092T Expired - Lifetime DE69334092T2 (de) 1992-08-04 1993-08-04 Nachweis von Mycobacterium tuberculosis durch Vervielfältigung von Nukleinsäuresequenzen
DE69329636T Expired - Lifetime DE69329636T2 (de) 1992-08-04 1993-08-04 Verfahren zur Vervielfältigung von Nukleinsäuresequenzen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69334092T Expired - Lifetime DE69334092T2 (de) 1992-08-04 1993-08-04 Nachweis von Mycobacterium tuberculosis durch Vervielfältigung von Nukleinsäuresequenzen

Country Status (11)

Country Link
EP (2) EP0978570B1 (de)
JP (3) JP4372837B2 (de)
KR (1) KR100316498B1 (de)
AT (2) ATE197479T1 (de)
AU (2) AU670116B2 (de)
CA (1) CA2141430C (de)
DE (2) DE69334092T2 (de)
ES (1) ES2277679T3 (de)
NO (1) NO950381L (de)
TW (1) TW278099B (de)
WO (1) WO1994003472A1 (de)

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WO1995015399A1 (fr) * 1993-12-01 1995-06-08 Toyo Boseki Kabushiki Kaisha Procede d'amplification et de detection d'une sequence nucleotidique au moyen d'enzymes thermostables
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
ES2150103T3 (es) * 1995-01-19 2000-11-16 Gen Probe Inc Oligonucleotidos de amplificacion de acido nucleico y sondas para borrelia asociados con la enfermedad de lyme.
US5622827A (en) * 1995-02-07 1997-04-22 Gen-Probe Incorporated Amplification primers and nucleic acid probes targeted to coccidioides immitis nucleic acid
WO1996034983A1 (en) 1995-05-05 1996-11-07 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
US5925518A (en) * 1995-05-19 1999-07-20 Akzo Nobel N.V. Nucleic acid primers for amplification of a mycobacteria RNA template
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US5739311A (en) * 1995-06-07 1998-04-14 Gen-Probe Incorporated Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
US5747252A (en) 1995-06-07 1998-05-05 Gen-Probe Incorporated Nucleic acid probes and amplification oligonucleotides for Neisseria species
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
JP4293635B2 (ja) * 1995-06-07 2009-07-08 ジェン−プローブ・インコーポレーテッド Ｎｅｉｓｓｅｒｉａ種用の核酸プローブおよび増幅オリゴヌクレオチド
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
AU709292B2 (en) * 1995-06-07 1999-08-26 Gen-Probe Incorporated Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5652126A (en) * 1995-06-07 1997-07-29 Gen-Probe Incorporated Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
ATE309387T1 (de) * 1996-03-01 2005-11-15 Du Pont Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments
AU710996B2 (en) 1996-05-22 1999-10-07 Gen-Probe Incorporated Compositions and methods for the detection of mycobacterium kansasii
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
US6379929B1 (en) 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
GB9626074D0 (en) 1996-12-16 1997-02-05 Cytocell Ltd Nucleic acids amplification assay
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
WO1998050583A1 (en) 1997-05-02 1998-11-12 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
ATE320507T1 (de) * 1997-08-14 2006-04-15 Takara Bio Inc Methoden zur dns vervielfältigung und material dafür
JP2002509443A (ja) * 1997-10-31 2002-03-26 ジェン−プローブ・インコーポレーテッド 核酸検出法
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法
WO2000070039A1 (en) * 1999-05-17 2000-11-23 Mcgill University METHOD FOR SUBTRACTING cDNAs BY SUPPRESSING THE SYNTHESIS OF SPECIFICALLY TARGETED mRNAs
AU781570B2 (en) 1999-07-23 2005-06-02 Gen-Probe Incorporated Polynucleotide amplification method
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
JP2002051778A (ja) * 2000-05-31 2002-02-19 Tosoh Corp 小型球形ウイルス（ｓｒｓｖ）ｒｎａ検出のためのオリゴヌクレオチドおよび検出法
AU2001291513B2 (en) * 2000-10-03 2007-06-07 Id+Plus Ltd Nucleotide detection method
AUPR050700A0 (en) * 2000-10-03 2000-10-26 Id+Plus Ltd Detection method
AU3054502A (en) 2000-10-23 2002-05-06 Gen Probe Inc Compositions and methods for detecting human immunodeficiency virus 2 (hiv-2)
JP2002125688A (ja) * 2000-10-30 2002-05-08 Tosoh Corp Ｃ型肝炎ウイルスの高感度検出のためのオリゴヌクレオチドおよび検出法
DE10119203B4 (de) * 2001-04-19 2004-09-16 Rolf Dr. Postina Verfahren zur Erzeugung von doppelsträngigen Nukleinsäuren mit einzelsträngigen Überhängen beliebiger Länge und Nukleotidzusammensetzung
AU2003253651C1 (en) 2002-06-14 2010-06-03 Gen-Probe Incorporated Compositions and methods for detecting hepatitis B virus
EP3241915B1 (de) 2002-10-16 2021-06-23 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis des west-nil-viruses
JP4699384B2 (ja) 2003-12-19 2011-06-08 ジェン−プロウブ インコーポレイテッド Ｈｉｖ−１およびｈｉｖ−２の核酸を検出するための組成物、方法およびキット
US20060068380A1 (en) 2004-09-30 2006-03-30 Gen-Probe Incorporated Assay for detecting and quantifying HIV-1
CA2582661C (en) 2004-11-09 2015-08-11 Gen-Probe Incorporated Compositions and methods for detecting group a streptococci
AU2006214141B2 (en) 2005-02-18 2010-07-15 Gen-Probe Incorporated Sample preparation method incorporating an alkaline shock
CA2605015C (en) 2005-05-06 2013-09-10 Gen-Probe Incorporated Compositions and assays to detect influenza virus a and b nucleic acids
AU2006315489B2 (en) 2005-11-14 2012-09-06 Gen-Probe Incorporated Parametric calibration method
AU2007267517B2 (en) * 2006-05-24 2012-12-20 Gen-Probe Incorporated Probes and kits for determining the presence of mycobacterium tuberculosis complex organisms in a test sample and method of amplifying gram-positive bacteria and fungi employing capture probe
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GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
EP1978111B1 (de) * 2007-04-02 2013-03-27 Gen-Probe Incorporated Zusammensetzungen, Kits und zugehörige Verfahren zur Erkennung und/oder Überwachung von Pseudomonas aeruginosa
EP3957754A1 (de) 2008-04-21 2022-02-23 Gen-Probe Incorporated Verfahren zur erkennung des chikungunya-virus
EP3118208A1 (de) 2009-02-26 2017-01-18 Gen-Probe Incorporated Assay zum nachweisen menschlicher parvovirennukleinsäure
US9234249B2 (en) 2010-07-12 2016-01-12 Gen-Probe Incorporated Compositions and assays to detect swine H1N1 influenza A virus, seasonal H1 influenza A virus and seasonal H3 influenza A virus nucleic acids
EP2611932A2 (de) 2010-08-30 2013-07-10 Gen-Probe Incorporated Zusammensetzungen, verfahren und reaktionsmischungen für den nachweis von xenotropic murine leukemia virus-related virus (xmrv)
AU2012284307A1 (en) 2011-07-15 2013-05-09 Gen-Probe Incorporated Compositions and method for detecting human parvovirus nucleic acid and for detecting hepatitis A virus nucleic acids in single-plex or multiplex assays
US9932628B2 (en) 2012-07-27 2018-04-03 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
CA2899645C (en) 2013-03-15 2022-08-30 Gen-Probe Incorporated Calibration method, apparatus and computer program product
WO2018175883A1 (en) 2017-03-24 2018-09-27 Gen-Probe Incorporated Compositions and methods for detecting or quantifying parainfluenza virus
EP4219771A3 (de) 2017-03-24 2023-08-16 Gen-Probe Incorporated Zusammensetzungen und verfahren für den multiplexnachweis viraler krankheitserreger in proben
WO2019032809A1 (en) 2017-08-11 2019-02-14 Gen-Probe Incorporated COMPOSITIONS AND METHODS FOR DETECTING STAPHYLOCOCCUS AUREUS
US20200318171A1 (en) 2017-12-15 2020-10-08 Gen-Probe Incorporated Compositions and Methods for Detecting Toxigenic Clostridium Difficile
GB2597567B (en) 2018-06-13 2022-11-23 Gen Probe Inc Compositions and methods for detecting group B streptococcus nucleic acid
EP3830302B1 (de) 2018-08-01 2022-10-05 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von nukleinsäuren des epstein-barr-virus
EP3841223A1 (de) 2018-08-08 2021-06-30 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits zum nachweis von mycoplasma genitalium
AU2019326462A1 (en) 2018-08-21 2021-03-25 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus
WO2020069085A2 (en) 2018-09-27 2020-04-02 Gen-Probe Incorporated Compositions and methods for detecting bordetella pertussis and bordetella parapertussis nucleic acid
JP2022508954A (ja) 2018-10-22 2022-01-19 ジェン－プローブ・インコーポレーテッド ヒトポリオーマウイルスｂｋウイルスを増幅、検出または定量化するための組成物および方法
AU2020335996A1 (en) 2019-08-23 2022-03-31 Gen-Probe Incorporated Compositions, methods and kits for detecting Treponema pallidum
CA3153514A1 (en) 2019-09-05 2021-03-11 Gen-Probe Incorporated Detection of chlamydia trachomatis nucleic acid variants
JP2023503946A (ja) 2019-12-09 2023-02-01 ジェン－プローブ・インコーポレーテッド 乾燥試料からのポリヌクレオチド分析物の定量化
WO2021178644A1 (en) 2020-03-04 2021-09-10 Gen-Probe Incorporated Compositions and methods for detecting sars-cov-2 nucleic acid
AU2022319876A1 (en) 2021-07-27 2024-01-18 Gen-Probe Incorporated Compositions and methods for detecting gastrointestinal pathogens
KR102544380B1 (ko) 2022-01-29 2023-06-16 (주)동방지퍼 지퍼용 슬라이더와 손잡이의 결착방법 및 이로 인해 제조된 지퍼
EP4282980A1 (de) 2022-05-23 2023-11-29 Mobidiag Oy Methoden zum verstärken einer nukleinsäure
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers

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Also Published As

Publication number Publication date
AU4792093A (en) 1994-03-03
NO950381D0 (no) 1995-02-02
JP3860809B2 (ja) 2006-12-20
JPH07509368A (ja) 1995-10-19
DE69329636T2 (de) 2001-03-01
JP2004194662A (ja) 2004-07-15
ATE346954T1 (de) 2006-12-15
ES2277679T3 (es) 2007-07-16
EP0587298A3 (en) 1995-08-30
NO950381L (no) 1995-04-03
CA2141430A1 (en) 1994-02-17
KR950702999A (ko) 1995-08-23
AU6802796A (en) 1996-12-19
TW278099B (de) 1996-06-11
AU670116B2 (en) 1996-07-04
CA2141430C (en) 2009-05-12
EP0978570B1 (de) 2006-11-29
KR100316498B1 (ko) 2002-06-20
EP0587298B1 (de) 2000-11-08
EP0587298A2 (de) 1994-03-16
DE69334092D1 (de) 2007-01-11
EP0978570A3 (de) 2000-04-12
DE69334092T2 (de) 2007-07-05
ATE197479T1 (de) 2000-11-11
EP0978570A2 (de) 2000-02-09
WO1994003472A1 (en) 1994-02-17
AU700253B2 (en) 1998-12-24
JP2006238892A (ja) 2006-09-14
JP4372837B2 (ja) 2009-11-25

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