ATE197479T1 - Verfahren zur vervielfältigung von nukleinsäuresequenzen - Google Patents

Verfahren zur vervielfältigung von nukleinsäuresequenzen

Info

Publication number
ATE197479T1
ATE197479T1 AT93306169T AT93306169T ATE197479T1 AT E197479 T1 ATE197479 T1 AT E197479T1 AT 93306169 T AT93306169 T AT 93306169T AT 93306169 T AT93306169 T AT 93306169T AT E197479 T1 ATE197479 T1 AT E197479T1
Authority
AT
Austria
Prior art keywords
duplication
nucleic acid
acid sequences
oligonucleotides
kits
Prior art date
Application number
AT93306169T
Other languages
English (en)
Inventor
Sherrol Mcdonough
Daniel Kacian
Nanibhushan Dattagupta
Diane Mcallister
Philip Hammond
Thomas Ryder
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ATE197479T1 publication Critical patent/ATE197479T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT93306169T 1992-08-04 1993-08-04 Verfahren zur vervielfältigung von nukleinsäuresequenzen ATE197479T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US92540592A 1992-08-04 1992-08-04

Publications (1)

Publication Number Publication Date
ATE197479T1 true ATE197479T1 (de) 2000-11-11

Family

ID=25451694

Family Applications (2)

Application Number Title Priority Date Filing Date
AT99121906T ATE346954T1 (de) 1992-08-04 1993-08-04 Nachweis von mycobacterium tuberculosis durch vervielfältigung von nukleinsäuresequenzen
AT93306169T ATE197479T1 (de) 1992-08-04 1993-08-04 Verfahren zur vervielfältigung von nukleinsäuresequenzen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT99121906T ATE346954T1 (de) 1992-08-04 1993-08-04 Nachweis von mycobacterium tuberculosis durch vervielfältigung von nukleinsäuresequenzen

Country Status (11)

Country Link
EP (2) EP0978570B1 (de)
JP (3) JP4372837B2 (de)
KR (1) KR100316498B1 (de)
AT (2) ATE346954T1 (de)
AU (2) AU670116B2 (de)
CA (1) CA2141430C (de)
DE (2) DE69329636T2 (de)
ES (1) ES2277679T3 (de)
NO (1) NO950381L (de)
TW (1) TW278099B (de)
WO (1) WO1994003472A1 (de)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981179A (en) * 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
EP1251182A3 (de) 1993-12-01 2002-11-27 Toyo Boseki Kabushiki Kaisha Verfahren zur Amplifikation und Erkennung von Nukleinsäure-Zielsequenzen unter Verwendung von thermostabilen Enzymen
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
AU689375B2 (en) * 1995-01-19 1998-03-26 Gen-Probe Incorporated Nucleic acid amplification oligonucleotides and probes to lyme disease associated borrelia
US5622827A (en) * 1995-02-07 1997-04-22 Gen-Probe Incorporated Amplification primers and nucleic acid probes targeted to coccidioides immitis nucleic acid
WO1996034983A1 (en) 1995-05-05 1996-11-07 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
US5925518A (en) * 1995-05-19 1999-07-20 Akzo Nobel N.V. Nucleic acid primers for amplification of a mycobacteria RNA template
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
US5739311A (en) * 1995-06-07 1998-04-14 Gen-Probe Incorporated Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US5652126A (en) * 1995-06-07 1997-07-29 Gen-Probe Incorporated Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5747252A (en) * 1995-06-07 1998-05-05 Gen-Probe Incorporated Nucleic acid probes and amplification oligonucleotides for Neisseria species
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
WO1996040990A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
WO1996041017A2 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Nucleic acid probes and amplification oligonucleotides for neisseria species
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
CA2246238A1 (en) * 1996-03-01 1997-09-04 E.I. Du Pont De Nemours And Company A method for the amplification and detection of a nucleic acid fragment of interest
CA2255624C (en) 1996-05-22 2007-09-04 Gen-Probe Incorporated Compositions and methods for the detection of mycobacterium kansasii
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
WO1998022625A1 (en) 1996-11-20 1998-05-28 The Regents Of The University Of Michigan Microfabricated isothermal nucleic acid amplification devices and methods
GB9626074D0 (en) 1996-12-16 1997-02-05 Cytocell Ltd Nucleic acids amplification assay
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
AU738708B2 (en) 1997-05-02 2001-09-27 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
DE69833864T2 (de) * 1997-08-14 2006-12-21 Takara Bio Inc., Otsu Methoden zur dns vervielfältigung und material dafür
AU1450699A (en) * 1997-10-31 1999-05-24 Gen-Probe Incorporated Methods of nucleic acid detection
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法
AU4739600A (en) * 1999-05-17 2000-12-05 Mcgill University Method for subtracting cdnas by suppressing the synthesis of specifically targeted mrnas
AU781570B2 (en) 1999-07-23 2005-06-02 Gen-Probe Incorporated Polynucleotide amplification method
US6902891B2 (en) 1999-12-17 2005-06-07 Bio Merieux Process for labeling a nucleic acid
US6489114B2 (en) 1999-12-17 2002-12-03 Bio Merieux Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
JP2002051778A (ja) * 2000-05-31 2002-02-19 Tosoh Corp 小型球形ウイルス（ｓｒｓｖ）ｒｎａ検出のためのオリゴヌクレオチドおよび検出法
AUPR050700A0 (en) * 2000-10-03 2000-10-26 Id+Plus Ltd Detection method
AU2001291513B2 (en) * 2000-10-03 2007-06-07 Id+Plus Ltd Nucleotide detection method
US6870045B2 (en) 2000-10-23 2005-03-22 Gen-Probe Incorporated Kits for detecting HIV-2
JP2002125688A (ja) * 2000-10-30 2002-05-08 Tosoh Corp Ｃ型肝炎ウイルスの高感度検出のためのオリゴヌクレオチドおよび検出法
DE10119203B4 (de) * 2001-04-19 2004-09-16 Rolf Dr. Postina Verfahren zur Erzeugung von doppelsträngigen Nukleinsäuren mit einzelsträngigen Überhängen beliebiger Länge und Nukleotidzusammensetzung
EP1513958B1 (de) 2002-06-14 2011-08-10 Gen-Probe Incorporated Zusammensetzungen zum nachweis von hepatitis-b-virus
EP1583949B1 (de) 2002-10-16 2015-06-17 Gen-Probe Incorporated Zusammensetzungen und verfahren zumnachweis des west-nil-virus
ES2383947T3 (es) 2003-12-19 2012-06-27 Gen-Probe Incorporated Composiciones, métodos y equipos para la detección de ácidos nucleicos del vih-1 y vih-2
CA2847930C (en) 2004-09-30 2016-07-05 Gen-Probe Incorporated Assay for detecting and quantifying hiv-1
AU2005333163B2 (en) 2004-11-09 2011-06-16 Gen-Probe Incorporated Compositions and methods for detecting Group A streptococci
US7510837B2 (en) 2005-02-18 2009-03-31 Gen-Probe Incorporated Sample preparation method incorporating an alkaline shock
US8124335B2 (en) 2005-05-06 2012-02-28 Gen-Probe Incorporated Compositions and assays to detect influenza virus A and B nucleic acids
CA2627259C (en) 2005-11-14 2016-02-23 Gen-Probe Incorporated Parametric calibration method
EP2027293B1 (de) * 2006-05-24 2014-11-19 Gen-Probe Incorporated Verfahren zum lysieren von mycobacterium und amplifikation seiner nukleinsäuren
EP1921156A1 (de) * 2006-11-10 2008-05-14 bioMerieux B.V. Verbesserte multiplexe Nukleinsäureamplifikation mittels blockierter Primer
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
EP1978111B1 (de) * 2007-04-02 2013-03-27 Gen-Probe Incorporated Zusammensetzungen, Kits und zugehörige Verfahren zur Erkennung und/oder Überwachung von Pseudomonas aeruginosa
CA3128538C (en) 2008-04-21 2023-08-08 Gen-Probe Incorporated Method for detecting chikungunya virus
EP3257859B1 (de) 2009-02-26 2020-09-23 Gen-Probe Incorporated Assay zum nachweisen menschlicher parvoviren-nukleinsäure
EP2593569B1 (de) 2010-07-12 2018-01-03 Gen-Probe Incorporated Zusammensetzungen und tests für den nachweis von nukleinsäuren des saisonalen h1 influenza a virus
WO2012030856A2 (en) 2010-08-30 2012-03-08 Gen-Probe Incorporated Compositions, methods and reaction mixtures for the detection of xenotropic murine leukemia virus-related virus
JP6150795B2 (ja) 2011-07-15 2017-06-28 ジェン−プロウブ インコーポレイテッド シングルプレックス又はマルチプレックスアッセイにおいてヒトパルボウイルス核酸を検出及びａ型肝炎ウイルス核酸を検出するための組成物及び方法
US9932628B2 (en) 2012-07-27 2018-04-03 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
AU2014228343B2 (en) 2013-03-15 2019-11-07 Gen-Probe Incorporated Calibration method, apparatus and computer program product
WO2018175883A1 (en) 2017-03-24 2018-09-27 Gen-Probe Incorporated Compositions and methods for detecting or quantifying parainfluenza virus
CA3176517C (en) 2017-03-24 2024-03-05 Gen-Probe Incorporated Compositions and methods for detection of viral pathogens in samples
EP3665309B1 (de) 2017-08-11 2023-02-22 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von staphylococcus aureus
EP3724354A1 (de) 2017-12-15 2020-10-21 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von toxigenem clostridium difficile
GB2590210B (en) 2018-06-13 2023-01-25 Gen Probe Inc Compositions and methods for detecting group B Streptococcus nucleic acid
JP7469290B2 (ja) 2018-08-01 2024-04-16 ジェン－プローブ・インコーポレーテッド エプスタイン－バールウイルスの核酸を検出するための組成物および方法
EP3841223A1 (de) 2018-08-08 2021-06-30 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits zum nachweis von mycoplasma genitalium
WO2020041414A1 (en) 2018-08-21 2020-02-27 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus
WO2020069085A2 (en) 2018-09-27 2020-04-02 Gen-Probe Incorporated Compositions and methods for detecting bordetella pertussis and bordetella parapertussis nucleic acid
CA3116895A1 (en) 2018-10-22 2020-04-30 Gen-Probe Incorporated Compositions and methods for amplifying, detecting or quantifying human polyomavirus bk virus
CA3152309A1 (en) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
US20220333162A1 (en) 2019-09-05 2022-10-20 Gen-Probe Incorporated Detection of chlamydia trachomatis nucleic acid variants
EP4361284A3 (de) 2019-12-09 2024-05-29 Gen-Probe Incorporated Quantifizierung von polynukleotidanalyten aus getrockneten proben
EP4114985A1 (de) 2020-03-04 2023-01-11 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von sars-cov-2-nukleinsäure
WO2023010008A1 (en) 2021-07-27 2023-02-02 Gen-Probe Incorporated Compositions and methods for detecting gastrointestinal pathogens
KR102544380B1 (ko) 2022-01-29 2023-06-16 (주)동방지퍼 지퍼용 슬라이더와 손잡이의 결착방법 및 이로 인해 제조된 지퍼
EP4282980A1 (de) 2022-05-23 2023-11-29 Mobidiag Oy Methoden zum verstärken einer nukleinsäure
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988003957A1 (en) * 1986-11-24 1988-06-02 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
ATE141956T1 (de) * 1989-07-11 1996-09-15 Gen Probe Inc Verfahren zur vervielfältigung von nukleinsäuresequenzen
EP0439182B1 (de) * 1990-01-26 1996-04-24 Abbott Laboratories Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
WO1992007957A1 (en) * 1990-10-31 1992-05-14 Cimino George D Methods and compositions for minimizing inhibition of nucleic acid amplification
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5521300A (en) * 1991-08-13 1996-05-28 Norval B. Galloway Oligonucleotides complementary to mycobacterial nucleic acids
WO1993022461A1 (en) * 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit

Also Published As

Publication number Publication date
KR950702999A (ko) 1995-08-23
JP4372837B2 (ja) 2009-11-25
EP0978570A2 (de) 2000-02-09
TW278099B (de) 1996-06-11
ATE346954T1 (de) 2006-12-15
EP0587298B1 (de) 2000-11-08
DE69334092D1 (de) 2007-01-11
JP2006238892A (ja) 2006-09-14
AU4792093A (en) 1994-03-03
JP3860809B2 (ja) 2006-12-20
EP0587298A2 (de) 1994-03-16
ES2277679T3 (es) 2007-07-16
EP0978570B1 (de) 2006-11-29
AU670116B2 (en) 1996-07-04
DE69329636T2 (de) 2001-03-01
DE69334092T2 (de) 2007-07-05
DE69329636D1 (de) 2000-12-14
CA2141430C (en) 2009-05-12
AU700253B2 (en) 1998-12-24
NO950381D0 (no) 1995-02-02
CA2141430A1 (en) 1994-02-17
KR100316498B1 (ko) 2002-06-20
JPH07509368A (ja) 1995-10-19
NO950381L (no) 1995-04-03
JP2004194662A (ja) 2004-07-15
EP0587298A3 (en) 1995-08-30
EP0978570A3 (de) 2000-04-12
AU6802796A (en) 1996-12-19
WO1994003472A1 (en) 1994-02-17

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