AU4739600A - Method for subtracting cdnas by suppressing the synthesis of specifically targeted mrnas - Google Patents

Method for subtracting cdnas by suppressing the synthesis of specifically targeted mrnas

Info

Publication number
AU4739600A
AU4739600A AU47396/00A AU4739600A AU4739600A AU 4739600 A AU4739600 A AU 4739600A AU 47396/00 A AU47396/00 A AU 47396/00A AU 4739600 A AU4739600 A AU 4739600A AU 4739600 A AU4739600 A AU 4739600A
Authority
AU
Australia
Prior art keywords
suppressing
synthesis
specifically targeted
targeted mrnas
cdnas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU47396/00A
Inventor
Jerry Pelletier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
McGill University
Original Assignee
McGill University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by McGill University filed Critical McGill University
Publication of AU4739600A publication Critical patent/AU4739600A/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU47396/00A 1999-05-17 2000-05-17 Method for subtracting cdnas by suppressing the synthesis of specifically targeted mrnas Abandoned AU4739600A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13454899P 1999-05-17 1999-05-17
US60134548 1999-05-17
PCT/CA2000/000586 WO2000070039A1 (en) 1999-05-17 2000-05-17 METHOD FOR SUBTRACTING cDNAs BY SUPPRESSING THE SYNTHESIS OF SPECIFICALLY TARGETED mRNAs

Publications (1)

Publication Number Publication Date
AU4739600A true AU4739600A (en) 2000-12-05

Family

ID=22463856

Family Applications (1)

Application Number Title Priority Date Filing Date
AU47396/00A Abandoned AU4739600A (en) 1999-05-17 2000-05-17 Method for subtracting cdnas by suppressing the synthesis of specifically targeted mrnas

Country Status (2)

Country Link
AU (1) AU4739600A (en)
WO (1) WO2000070039A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105861487B (en) 2012-01-26 2020-05-05 纽亘技术公司 Compositions and methods for targeted nucleic acid sequence enrichment and efficient library generation
CN104619894B (en) 2012-06-18 2017-06-06 纽亘技术公司 For the composition and method of the Solid phase of unexpected nucleotide sequence
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
CN105849264B (en) 2013-11-13 2019-09-27 纽亘技术公司 For identifying the composition and method that repeat sequencing reading
SG11201604198YA (en) 2013-12-30 2016-07-28 Curevac Ag Methods for rna analysis
EP3090060B1 (en) * 2013-12-30 2019-02-20 CureVac AG Methods for rna analysis
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
ES2890677T3 (en) * 2014-03-19 2022-01-21 Ionis Pharmaceuticals Inc Compositions for Modulating Ataxin 2 Expression
CN107075581B (en) 2014-08-06 2022-03-18 纽亘技术公司 Digital measurement by targeted sequencing
WO2017001058A1 (en) * 2015-07-01 2017-01-05 Curevac Ag Method for analysis of an rna molecule
CN109415727A (en) * 2016-06-13 2019-03-01 格里尔公司 The cell-free nucleic acid of mutation is enriched with for the method for cancer detection
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
AU2019310097A1 (en) 2018-07-25 2021-02-04 Ionis Pharmaceuticals, Inc. Compounds and methods for reducing ATXN2 expression
US11680261B2 (en) 2018-11-15 2023-06-20 Grail, Inc. Needle-based devices and methods for in vivo diagnostics of disease conditions

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989005358A1 (en) * 1987-11-30 1989-06-15 University Of Iowa Research Foundation Dna and rna molecules stabilized by modifications of the 3'-terminal phosphodiester linkage and their use as nucleic acid probes and as therapeutic agents to block the expression of specifically targeted genes
CA2005252C (en) * 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
KR100316498B1 (en) * 1992-08-04 2002-06-20 다니엘 엘. 캐시앙, 헨리 엘. 노르호프, 피터 알. 쉬어리 Nucleic Acid Sequence Amplification Method
US5891637A (en) * 1996-10-15 1999-04-06 Genentech, Inc. Construction of full length cDNA libraries

Also Published As

Publication number Publication date
WO2000070039A1 (en) 2000-11-23

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase