ES2059627T3 - Precursores de insulina, su preparacion, y secuencias de adn, vehiculos de expresion y cebadores, y un procedimiento para producir insulina humana y analogos. - Google Patents

Precursores de insulina, su preparacion, y secuencias de adn, vehiculos de expresion y cebadores, y un procedimiento para producir insulina humana y analogos.

Info

Publication number
ES2059627T3
ES2059627T3 ES89111221T ES89111221T ES2059627T3 ES 2059627 T3 ES2059627 T3 ES 2059627T3 ES 89111221 T ES89111221 T ES 89111221T ES 89111221 T ES89111221 T ES 89111221T ES 2059627 T3 ES2059627 T3 ES 2059627T3
Authority
ES
Spain
Prior art keywords
insulin
amino acid
precursors
human insulin
acid residues
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89111221T
Other languages
English (en)
Inventor
Ib Jonassen
Ib Groth Clausen
Ejner Bech Jensen
Allan Svendsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2059627T3 publication Critical patent/ES2059627T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

LA INVENCION SE REFIERE A PRECURSORES DE INSULINA QUE TIENEN LA SECUENCIA DE AMINOACIDOS B(1 - 29) - X1 - X2 - Y2 - Y1 - A(1 21), EN LA QUE B(1 - 29) SON LOS 29 RESIDUOS DE AMINOACIDOS DE LA CADENA B DE LA INSULINA HUMANA, A(1 - 21) SON LOS 21 RESIDUOS DE AMINOACIDOS DE LA CADENA A DE LA INSULINA HUMAN, X1 ES UN ENLACE PEPTIDICO O UNO O MAS RESIDUOS AMINOACIDOS, X2 ES GLU O ASP, E Y1 Y Y2 SON LYS O ARG, LAS POSICIONES A6 Y A11, A7 Y B7 Y A20 Y B19, RESPECTIVAMENTE ESTAN CONECTADAS POR PUENTES DE ASUFRE, Y OPCIONALMENTE UNO O MAS DE LOS RESIDUOS AMINOACIDOS DE LAS CADENAS B(1 - 29) Y A(1 - 21) ESTA SUSTITUIDO POR OTRO RESIDUO AMINOACIDO. LOS PRECURSORES DE INSULINA SE PREPARAN CULTIVANDO UNA CEPA DE LEVADURA TRANSFORMADA CON UN VEHICULO DE EXPRESION REPLICABLE QUE COMPRENDE UNA SECUENCIA DE DNA QUE CODIFICA AL PRECURSOR DE INSULINA, Y AISLANDOLO DEL MEDIO DE CULTIVO. LOS PREECURSORES DE INSULINA SE PUEDEN CONVERTIR EN INSULINA HUMAN O EN ANALOGOS DE INSULINA POR TRATAMIENTO ENZIMATICO.
ES89111221T 1988-06-20 1989-06-20 Precursores de insulina, su preparacion, y secuencias de adn, vehiculos de expresion y cebadores, y un procedimiento para producir insulina humana y analogos. Expired - Lifetime ES2059627T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK336188A DK336188D0 (da) 1988-06-20 1988-06-20 Propeptider

Publications (1)

Publication Number Publication Date
ES2059627T3 true ES2059627T3 (es) 1994-11-16

Family

ID=8122004

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89111221T Expired - Lifetime ES2059627T3 (es) 1988-06-20 1989-06-20 Precursores de insulina, su preparacion, y secuencias de adn, vehiculos de expresion y cebadores, y un procedimiento para producir insulina humana y analogos.

Country Status (17)

Country Link
US (2) US5202415A (es)
EP (1) EP0347845B1 (es)
JP (1) JP2766999B2 (es)
KR (1) KR0140238B1 (es)
AT (1) ATE93239T1 (es)
AU (1) AU618612B2 (es)
CA (1) CA1337976C (es)
DE (1) DE68908491T2 (es)
DK (1) DK336188D0 (es)
ES (1) ES2059627T3 (es)
FI (1) FI102294B (es)
IE (1) IE64303B1 (es)
NO (1) NO177499C (es)
NZ (1) NZ229615A (es)
PT (1) PT90897B (es)
WO (1) WO1989012647A1 (es)
ZA (1) ZA894639B (es)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
DK134189D0 (da) * 1989-03-20 1989-03-20 Nordisk Gentofte Insulinforbindelser
DK72793D0 (da) * 1993-06-21 1993-06-21 Novo Nordisk As Nyt produkt
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
KR0150565B1 (ko) * 1995-02-15 1998-08-17 김정재 유전자 조환에 의한 사람 인슐린 전구체의 제조 및 이를 이용한 인슐린의 제조방법
GB9513967D0 (en) * 1995-07-08 1995-09-06 Univ Leicester Insulin
KR100253916B1 (ko) * 1997-12-29 2000-05-01 김충환 사람 인슐린 전구체의 제조방법
US7169889B1 (en) 1999-06-19 2007-01-30 Biocon Limited Insulin prodrugs hydrolyzable in vivo to yield peglylated insulin
US6777207B2 (en) 1999-12-29 2004-08-17 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US7378390B2 (en) 1999-12-29 2008-05-27 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US6521738B2 (en) 1999-12-29 2003-02-18 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogs
WO2001049870A1 (en) 1999-12-29 2001-07-12 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
KR100449454B1 (ko) * 2000-10-02 2004-09-21 이현철 단일사슬 인슐린 유도체의 유전자를 포함하는 당뇨병치료용 벡터
KR20030005204A (ko) * 2000-12-25 2003-01-17 가부시키가이샤 시세이도 교감신경 활성화 향료 조성물
US7060675B2 (en) * 2001-02-15 2006-06-13 Nobex Corporation Methods of treating diabetes mellitus
US6867183B2 (en) * 2001-02-15 2005-03-15 Nobex Corporation Pharmaceutical compositions of insulin drug-oligomer conjugates and methods of treating diseases therewith
WO2002079251A2 (en) * 2001-04-02 2002-10-10 Novo Nordisk A/S Method for making human insulin precursors
US7105314B2 (en) 2001-04-02 2006-09-12 Novo Nordisk A/S Method for making human insulin precursors
ATE443082T1 (de) * 2001-04-02 2009-10-15 Novo Nordisk As Insulinvorstufen und verfahren zu deren herstellung
US6858580B2 (en) * 2001-06-04 2005-02-22 Nobex Corporation Mixtures of drug-oligomer conjugates comprising polyalkylene glycol, uses thereof, and methods of making same
US6713452B2 (en) * 2001-06-04 2004-03-30 Nobex Corporation Mixtures of calcitonin drug-oligomer conjugates comprising polyalkylene glycol, uses thereof, and methods of making same
US7713932B2 (en) 2001-06-04 2010-05-11 Biocon Limited Calcitonin drug-oligomer conjugates, and uses thereof
US6835802B2 (en) * 2001-06-04 2004-12-28 Nobex Corporation Methods of synthesizing substantially monodispersed mixtures of polymers having polyethylene glycol moieties
US6828297B2 (en) * 2001-06-04 2004-12-07 Nobex Corporation Mixtures of insulin drug-oligomer conjugates comprising polyalkylene glycol, uses thereof, and methods of making same
US6828305B2 (en) * 2001-06-04 2004-12-07 Nobex Corporation Mixtures of growth hormone drug-oligomer conjugates comprising polyalkylene glycol, uses thereof, and methods of making same
AU2002316807A1 (en) * 2001-06-08 2002-12-23 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogs
US20030040601A1 (en) * 2001-06-08 2003-02-27 Ivan Diers Method for making insulin precursors and insulin analog precursors
US7196059B2 (en) * 2001-09-07 2007-03-27 Biocon Limited Pharmaceutical compositions of insulin drug-oligomer conjugates and methods of treating diseases therewith
US7166571B2 (en) * 2001-09-07 2007-01-23 Biocon Limited Insulin polypeptide-oligomer conjugates, proinsulin polypeptide-oligomer conjugates and methods of synthesizing same
US7312192B2 (en) * 2001-09-07 2007-12-25 Biocon Limited Insulin polypeptide-oligomer conjugates, proinsulin polypeptide-oligomer conjugates and methods of synthesizing same
US6913903B2 (en) * 2001-09-07 2005-07-05 Nobex Corporation Methods of synthesizing insulin polypeptide-oligomer conjugates, and proinsulin polypeptide-oligomer conjugates and methods of synthesizing same
US7601688B2 (en) * 2002-06-13 2009-10-13 Biocon Limited Methods of reducing hypoglycemic episodes in the treatment of diabetes mellitus
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
CN1836047B (zh) 2003-06-17 2010-12-22 赛姆生物系统遗传公司 在植物中生产胰岛素的方法
CA2580313C (en) * 2004-07-19 2016-03-15 Biocon Limited Insulin-oligomer conjugates, formulations and uses thereof
KR101699370B1 (ko) 2006-09-22 2017-02-14 노보 노르디스크 에이/에스 프로테아제 내성 인슐린 유사체
CN101939023B (zh) * 2007-10-16 2016-08-03 百康有限公司 可经口给药的固体药物组合物及其方法
WO2010149772A1 (en) 2009-06-26 2010-12-29 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and an amino acid
CN103249427A (zh) 2010-12-14 2013-08-14 诺沃—诺迪斯克有限公司 速效胰岛素联合长效胰岛素
RU2013130374A (ru) 2010-12-14 2015-01-20 Ново Нордиск А/С Препарат, содержащий инсулин, никотинамид и аминокислоту
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
EP4086279A4 (en) 2019-12-30 2024-08-07 Gan & Lee Pharmaceuticals Co Ltd INSULIN DERIVATIVE
AU2020418207A1 (en) 2019-12-30 2022-08-25 Gan & Lee Pharmaceuticals Co., Ltd. Long-acting GLP-1 compound
CN118574842A (zh) 2022-01-28 2024-08-30 甘李药业股份有限公司 酰化胰岛素

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RO81951B (ro) * 1980-03-27 1984-02-28 Eli Lilly And Company Procedeu de preparare a unui precursor de insulina
US4430266A (en) * 1980-11-28 1984-02-07 Eli Lilly And Company Process for producing an insulin precursor
US5015575A (en) * 1983-04-07 1991-05-14 Chiron Corporation Hybrid DNA synthesis of insulin
DE3327928A1 (de) * 1983-08-03 1985-02-21 Hoechst Ag, 6230 Frankfurt Verfahren zur herstellung von insulin-derivaten
DK58285D0 (da) * 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
DK347086D0 (da) * 1986-07-21 1986-07-21 Novo Industri As Novel peptides
US4946828A (en) * 1985-03-12 1990-08-07 Novo Nordisk A/S Novel insulin peptides
DK129385A (da) * 1985-03-22 1986-09-23 Novo Industri As Peptider og fremstilling deraf
DK437786D0 (da) * 1986-09-12 1986-09-12 Nordisk Gentofte Insulinprecursorer
KR900701842A (ko) * 1988-07-20 1990-12-04 헨리 브뢰늄 인간 인슐린 동족체와 그를 포함하는 제제
KR910700262A (ko) * 1988-12-23 1991-03-14 안네 제케르 사람 인슐린 유사체

Also Published As

Publication number Publication date
AU3860089A (en) 1990-01-12
FI102294B1 (fi) 1998-11-13
EP0347845A2 (en) 1989-12-27
FI102294B (fi) 1998-11-13
KR900701841A (ko) 1990-12-04
US5202415A (en) 1993-04-13
PT90897A (pt) 1989-12-29
PT90897B (pt) 1995-03-31
ZA894639B (en) 1991-12-24
CA1337976C (en) 1996-01-23
EP0347845B1 (en) 1993-08-18
FI906285A0 (fi) 1990-12-19
DK336188D0 (da) 1988-06-20
KR0140238B1 (en) 1998-07-01
EP0347845A3 (en) 1991-07-03
DE68908491D1 (de) 1993-09-23
ATE93239T1 (de) 1993-09-15
NO905470L (no) 1991-02-18
NZ229615A (en) 1991-03-26
NO905470D0 (no) 1990-12-19
IE891976L (en) 1989-12-20
NO177499B (no) 1995-06-19
NO177499C (no) 1995-09-27
WO1989012647A1 (en) 1989-12-28
IE64303B1 (en) 1995-07-26
JP2766999B2 (ja) 1998-06-18
JPH03505206A (ja) 1991-11-14
AU618612B2 (en) 1992-01-02
US5324641A (en) 1994-06-28
DE68908491T2 (de) 1994-01-20

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