ES2007110A6 - Un metodo para la preparacion de precursores de insulina. - Google Patents

Un metodo para la preparacion de precursores de insulina.

Info

Publication number
ES2007110A6
ES2007110A6 ES8702637A ES8702637A ES2007110A6 ES 2007110 A6 ES2007110 A6 ES 2007110A6 ES 8702637 A ES8702637 A ES 8702637A ES 8702637 A ES8702637 A ES 8702637A ES 2007110 A6 ES2007110 A6 ES 2007110A6
Authority
ES
Spain
Prior art keywords
precursors
amino acid
known manner
insulin
insulin precursors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8702637A
Other languages
English (en)
Inventor
Per Balschmidt
Finn Benned Hansen
Kim Ry Hejnaes
Ib Groth Clausen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nordisk Gentofte AS
Original Assignee
Nordisk Gentofte AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nordisk Gentofte AS filed Critical Nordisk Gentofte AS
Publication of ES2007110A6 publication Critical patent/ES2007110A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Obesity (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Emergency Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

UN METODO PARA LA PREPARACION DE PRECURSORES DE INSULINA, QUE COMPRENDE MODIFICAR LA HEBRA DE DNA CODIFICANTE DE LA PROINSULINA ENTERA, UNA FORMA MODIFICADA DE ESTA, O DE LA CADENA A Y B SEPARADAMENTE POR MUTAGENESIS IN VITRO PARA CODIFICAR UN PRECURSOR DE INSULINA QUE TIENE LA SECUENCIA DE AMINOACIDOS: PHE-VAL-ASN-GLN-HIS-LEU-CYS-GLY-SER-HIS-LEU-VAL-R-ALA-B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 B13 B14 LEU-TYR-LEU-VAL-CYS-GLY-R-ARG-GLY-PHE-TYR-THR-B15 B16 B17 B18 B19 B20 B21 B22 B23 B24 B25 B26 B27 PRO-LYS-XN-GLY-ILE-VAL-R-GLN-CYS-CYS-THR-SER-ILE-CYS-B28 B29 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 SER-LEU-TYR-GLN-LEU-R-ASN-TYR-CYS-ASN-A12 A13 A14 A15 A16 A17 A18 A19 A20 A21; INSERTANDO EL DNA MODIFICADO EN UN AGENTE DE EXPRESION REPLICABLE QUE CONTIENE UN PROMOTOR ADECUADO, TRANSFORMANDO ESTE SISTEMA EN UN ORGANISMO HUESPED, EXPRESANDO LA SECUENCIA DE DNA MODIFICADO Y AISLANDO EL PRECURSOR DE INSULINA DEL CULTIVO. LOS PRECURSORES SE PUEDEN CONVERTIR EN DERIVADOS DE INSULINA O SE PUEDEN UTILIZAREN PREPARADOS FARMACEUTICOS EN UNA MANERA CONOCIDA.
ES8702637A 1986-09-12 1987-09-11 Un metodo para la preparacion de precursores de insulina. Expired ES2007110A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK437786A DK437786D0 (da) 1986-09-12 1986-09-12 Insulinprecursorer

Publications (1)

Publication Number Publication Date
ES2007110A6 true ES2007110A6 (es) 1989-06-01

Family

ID=8132996

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8702637A Expired ES2007110A6 (es) 1986-09-12 1987-09-11 Un metodo para la preparacion de precursores de insulina.

Country Status (6)

Country Link
EP (1) EP0282550A1 (es)
JP (1) JPH01501150A (es)
AU (1) AU8028387A (es)
DK (1) DK437786D0 (es)
ES (1) ES2007110A6 (es)
WO (1) WO1988002005A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
DK336188D0 (da) * 1988-06-20 1988-06-20 Nordisk Gentofte Propeptider
DE58906966D1 (de) * 1988-06-23 1994-03-24 Hoechst Ag Mini-Proinsulin, seine Herstellung und Verwendung.
EP0425482B1 (en) * 1988-07-20 1993-08-18 Novo Nordisk A/S Human insulin analogs and preparations containing them
EP0367163B1 (de) * 1988-11-03 1996-01-03 Hoechst Aktiengesellschaft Verfahren zur Herstellung eines Insulin-Vorprodukts in Streptomyceten
DE3844211A1 (de) * 1988-12-29 1990-07-05 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
DK134189D0 (da) * 1989-03-20 1989-03-20 Nordisk Gentofte Insulinforbindelser

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
CA1204682A (en) * 1981-06-19 1986-05-20 Saran A. Narang Adaptors, and synthesis and cloning of proinsulin genes
DK58285D0 (da) * 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
DK113585D0 (da) * 1985-03-12 1985-03-12 Novo Industri As Nye peptider
DK119785D0 (da) * 1985-03-15 1985-03-15 Nordisk Gentofte Insulinpraeparat
DK129385A (da) * 1985-03-22 1986-09-23 Novo Industri As Peptider og fremstilling deraf
PH25772A (en) * 1985-08-30 1991-10-18 Novo Industri As Insulin analogues, process for their preparation

Also Published As

Publication number Publication date
EP0282550A1 (en) 1988-09-21
JPH01501150A (ja) 1989-04-20
WO1988002005A1 (en) 1988-03-24
DK437786D0 (da) 1986-09-12
AU8028387A (en) 1988-04-07

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19900912